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1
المصدر: FEMS Microbiology Ecology. 77:165-175
مصطلحات موضوعية: Genetics, Ecology, biology, Phylogenetic tree, macromolecular substances, Desulfurococcales, biology.organism_classification, 16S ribosomal RNA, Applied Microbiology and Biotechnology, Microbiology, Korarchaeota, Chaperonin, Thermoproteales, enzymes and coenzymes (carbohydrates), Crenarchaeota, biological sciences, bacteria, Archaea
الوصف: The diversity of archaeal communities growing in four hot springs (65–90 °C, pH 6.5) was assessed with 16S rRNA gene primers specific for the domain Archaea. Overall, mainly uncultured members of the Desulfurococcales, the Thermoproteales and the Korarchaeota, were identified. Based on this diversity, a set of chaperonin heat-shock protein (Hsp60) gene sequences from different archaeal species were aligned to design two degenerate primer sets for the amplification of the chaperonin gene: Ths and Kor (which can also detect the korarchaeotal chaperonin gene from one of the samples). A phylogenetic tree was constructed using the chaperonin sequences retrieved and other sequences from cultured representatives. The Alpha and Beta paralogs of the chaperonin gene were observed within the main clades and orthologs among them. Cultivated representatives from these clades were assigned to either paralog in the chaperonin tree. Uncultured representatives observed in the 16S rRNA gene analysis were found to be related to the Desulfurococcales. The topologies of the 16S rRNA gene and chaperonin phylogenetic trees were compared, and similar phylogenetic relationships were observed. Our results suggest that the chaperonin Hsp60 gene may be used as a phylogenetic marker for the clades found in this extreme environment.
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2
المؤلفون: Xavier P. Cristina, L. J. Garcia-Gil, C. A. Abella, Carles M. Borrego, Xavier Vila, Jordi B. Figueras
المصدر: FEMS Microbiology Ecology. 24:301-309
مصطلحات موضوعية: education.field_of_study, Ecology, Phototroph, biology, Population, Photosynthetic pigment, biology.organism_classification, Photosynthesis, Applied Microbiology and Biotechnology, Microbiology, chemistry.chemical_compound, Pigment, chemistry, visual_art, Green sulfur bacteria, Botany, visual_art.visual_art_medium, Bacteriochlorophyll, education, Bacteria
الوصف: The relative content of the four main bacteriochlorophyll (BChl) e homologs of several populations of brown-colored photosynthetic sulfur bacteria grown in different waterbodies have been measured by high performance liquid chromatography and statistically compared by principal component analysis. All the studied populations possessed representative pigment patterns enriched in highly alkylated bacteriochlorophyll e homologs, with average contents of 0.02±0.01%, 24.92±1.01%, 35.2±0.70%, and 39.9±0.71% for bacteriochlorophyll e1, e2, e3, and e4, respectively. These values clearly differ from those obtained for the same species growing under optimal conditions in laboratory batch cultures (4.99±1.11%, 50.34±1.73%, 28.99±0.63%, and 15.6±1.10% for bacteriochlorophyll e1, e2, e3, and e4, respectively). Multivariate statistical analyses grouped samples into two main clusters, both related to the developmental state of the population. Within these clusters, samples were arranged in several groups according to the physiological pigment response of bacterial populations to light limitation. Although bacteriochlorophyll homolog distribution cannot be considered a real taxonomic character, the data presented demonstrate that it can be useful in field studies since it reflects both the physiological status of the cells and the light regime under which the population has been growing.
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3
المؤلفون: Maria José Figueras, Krzysztof Ulfig, P. Vidal, Joan Cano, Josepa Gené, Josep Guarro, W. Łukasik
المصدر: FEMS Microbiology Ecology. 22:111-117
مصطلحات موضوعية: Ecology, Fungus, Contamination, Biology, biology.organism_classification, Applied Microbiology and Biotechnology, Microbiology, chemistry.chemical_compound, Mediterranean sea, chemistry, Nitrate, Botany, Ammonium, Trichophyton, Water pollution, Chrysosporium
الوصف: Sediments from the inflow of the river Tordera to the Mediterranean Sea (Catalonia, Spain) were examined for keratinolytic fungi. The horse hair baiting method was used. Out of 100 Petri dishes examined, 84 were found to be positive for keratinolytic fungi. Altogether, 169 of fungal strains belonging to 13 species were observed. Aphanoascusfulvescens (teleomorph+anamorph), Chrysosporium an. Arthroderma curreyi, Ch. keratinophilum, Ch. pannicola, Trichophyton ajelloi, and Ch. tropicum prevailed in the sediments. The qualitative and quantitative composition of keratinolytic fungi depended on both the natural and water pollution factors. The main factors influencing the keratinolytic mycoflora in sediments were: water temperature, pH, dissolved oxygen concentration, dissolved substances, phosphates, ammonium, nitrates, fecal bacterial contamination, BOD5, and strong summer insolation associated with low water levels.
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4دورية أكاديمية
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5دورية أكاديمية
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6دورية أكاديمية
المؤلفون: Vila, X, Abella, C.A, Figueras, J.B, Hurley, J.P
المصدر: FEMS Microbiology Ecology; Mar1998, Vol. 25 Issue 3, p287-299, 13p
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7دورية أكاديمية
المؤلفون: Borrego, C.M, Garcia-Gil, L.J, Vila, X, Cristina, X.P, Figueras, J.B, Abella, C.A
المصدر: FEMS Microbiology Ecology; Dec1997, Vol. 24 Issue 4, p301-309, 9p
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8دورية أكاديمية
المؤلفون: Ulfig, K, Guarro, J, Cano, J, Gené, J, Vidal, P, Figueras, M.J, Łukasik, W
المصدر: FEMS Microbiology Ecology; Feb1997, Vol. 22 Issue 2, p111-117, 7p
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9دورية أكاديمية
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