المؤلفون: Hsiao-Jung Kao, Ting-Yi Lin, Feng-Jen Hsieh, Jia-Ying Chien, Erh-Chan Yeh, Wan-Jia Lin, Yi-Hua Chen, Kai-Hsuan Ding, Yu Yang, Sheng-Chu Chi, Ping-Hsing Tsai, Chih-Chien Hsu, De-Kuang Hwang, Hsien-Yang Tsai, Mei-Ling Peng, Shi-Huang Lee, Siu-Fung Chau, Chen Yu Chen, Wai-Man Cheang, Shih-Jen Chen, Pui-Yan Kwok, Shih-Hwa Chiou, Mei-Yeh Jade Lu, Shun-Ping Huang

المصدر: npj Genomic Medicine, Vol 9, Iss 1, Pp 1-7 (2024)

مصطلحات موضوعية: Medicine, Genetics, QH426-470

الوصف: Abstract Our study presents a 319-gene panel targeting inherited retinal dystrophy (IRD) genes. Through a multi-center retrospective cohort study, we validated the assay’s effectiveness and clinical utility and characterized the mutation spectrum of Taiwanese IRD patients. Between January 2018 and May 2022, 493 patients in 425 unrelated families, all initially suspected of having IRD without prior genetic diagnoses, underwent detailed ophthalmic and physical examinations (with extra-ocular features recorded) and genetic testing with our customized panel. Disease-causing variants were identified by segregation analysis and clinical interpretation, with validation via Sanger sequencing. We achieved a read depth of >200× for 94.2% of the targeted 1.2 Mb region. 68.5% (291/425) of the probands received molecular diagnoses, with 53.9% (229/425) resolved cases. Retinitis pigmentosa (RP) is the most prevalent initial clinical impression (64.2%), and 90.8% of the cohort have the five most prevalent phenotypes (RP, cone-rod syndrome, Usher’s syndrome, Leber’s congenital amaurosis, Bietti crystalline dystrophy). The most commonly mutated genes of probands that received molecular diagnosis are USH2A (13.7% of the cohort), EYS (11.3%), CYP4V2 (4.8%), ABCA4 (4.5%), RPGR (3.4%), and RP1 (3.1%), collectively accounted for 40.8% of diagnoses. We identify 87 unique unreported variants previously not associated with IRD and refine clinical diagnoses for 21 patients (7.22% of positive cases). We developed a customized gene panel and tested it on the largest Taiwanese cohort, showing that it provides excellent coverage for diverse IRD phenotypes.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2056-7944

URL الوصول: https://doaj.org/article/483be3c5b3ae4fb894f541f92bf270b0

المؤلفون: Siu-Fung Chau, Chia-Yi Lee, Jing-Yang Huang, Ming-Chih Chou, Hung-Chi Chen, Shun-Fa Yang

المصدر: Medicina, Vol 56, Iss 11, p 621 (2020)

مصطلحات موضوعية: periodontal disease, eye, keratopathy, inflammation, epidemiology, Medicine (General), R5-920

الوصف: Background and objectives: We aimed to evaluate the correlation between periodontal disease (PD) and following ocular diseases via the National Health Insurance Research Database in Taiwan. Materials and Methods: A retrospective cohort study was conducted. Subjects were regarded as having PD according to the diagnostic codes. For comparison, each subject with PD was matched to one non-PD individual from the database after exclusion. The main outcome was defined as the development of infectious keratitis, endophthalmitis, orbital cellulitis, lacrimal duct infection, uveitis and infectious scleritis. Cox proportional hazard regression was used to yield the adjusted hazard ratios (aHR) of ocular diseases between the study and control groups. Results: A total of 426,594 subjects were enrolled in both the study and control groups. In the multivariable analysis, significantly higher rates of infectious keratitis (aHR: 1.094, 95% CI: 1.030–1.161), uveitis (aHR: 1.144, 95% CI: 1.074–1.218) and infectious scleritis (aHR: 1.270, 95% CI: 1.114–1.449) were found in the study group. Concerning the PD interval, infectious keratitis (aHR: 1.159, 95% CI: 1.041–1.291) and infectious scleritis (aHR: 1.345, 95% CI: 1.055–1.714) would significantly occur in PD patients with an interval shorter than two years, individuals with a PD interval that ranged from two to five years were under a higher risk of developing uveitis (aHR: 1.184, 95% CI: 1.065–1.315) and infectious scleritis (aHR: 1.386, 95% CI: 1.125–1.708), and the rate of uveitis (aHR: 1.149, 95% CI: 1.038–1.272) was significantly higher if PD persisted more than five years. Conclusions: The presence of PD was moderately associated with the risk of developing infectious keratitis, uveitis and infectious scleritis.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/1648-9144/56/11/621; https://doaj.org/toc/1010-660X; https://doaj.org/toc/1648-9144

URL الوصول: https://doaj.org/article/a7363dbc83d84733b0bc3ea12bf9db6d

المؤلفون: Sung-Ying Huang, Shu-Fang Chang, Siu-Fung Chau, Sheng-Chun Chiu

المصدر: Biomolecules, Vol 9, Iss 8, p 380 (2019)

مصطلحات موضوعية: ARPE-19, hispidin, hydrogen peroxide, Nrf2, oxidative stress, age-related macular degeneration, Microbiology, QR1-502

الوصف: Hispidin, a polyphenol compound isolated from Phellinus linteus, has been reported to possess antioxidant activities. In this study, we aimed to investigate the mechanisms underlying the protective effect of hispidin against hydrogen peroxide (H2O2)-induced oxidative stress on Adult Retinal Pigment Epithelial cell line-19 (ARPE-19) cells. Hispidin was not cytotoxic to ARPE-19 cells at concentrations of less than 50 μM. The levels of intracellular reactive oxygen species (ROS) were analyzed by dichlorofluorescin diacetate (DCFDA) staining. Hispidin significantly restored H2O2-induced cell death and reduced the levels of intracellular ROS. The expression levels of antioxidant enzymes, such as NAD(P)H:Quinine oxidoreductase-1 (NQO-1), heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), and glutamate-cysteine ligase modifier subunit (GCLM) were examined using real-time PCR and Western blotting. Our results showed that hispidin markedly enhanced the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), HO-1, NQO-1, GCLM, and GCLC in a dose-dependent manner. Furthermore, knockdown experiments revealed that transfection with Nrf2 siRNA successfully suppresses the hispidin activated Nrf2 signaling in ARPE-19 cells. Moreover, activation of the c-Jun N-terminal kinase (JNK) pathway is involved in mediating the protective effects of hispidin on the ARPE-19 cells. Thus, the present study demonstrated that hispidin provides protection against H2O2-induced damage in ARPE-19 cells via activation of Nrf2 signaling and up-regulation of its downstream targets, including Phase II enzymes, which might be associated with the activation of the JNK pathway.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2218-273X/9/8/380; https://doaj.org/toc/2218-273X

URL الوصول: https://doaj.org/article/ac6fb111d39147fbbca31ffa9aa020db

المؤلفون: Hung-Chi Chen, Ming-Chih Chou, Jing-Yang Huang, Chia-Yi Lee, Siu-Fung Chau, Shun-Fa Yang

المصدر: Medicina
Medicina, Vol 56, Iss 621, p 621 (2020)
Medicina; Volume 56; Issue 11; Pages: 621
Volume 56
Issue 11

مصطلحات موضوعية: medicine.medical_specialty, periodontal disease, eye, keratopathy, inflammation, epidemiology, Medicine (General), Taiwan, Infectious Keratitis, Article, Cohort Studies, Endophthalmitis, R5-920, Risk Factors, Internal medicine, Epidemiology, medicine, Humans, Periodontal Diseases, Retrospective Studies, business.industry, Incidence, Hazard ratio, Retrospective cohort study, General Medicine, medicine.disease, Orbital cellulitis, business, Uveitis, Scleritis

الوصف: Background and objectives: We aimed to evaluate the correlation between periodontal disease (PD) and following ocular diseases via the National Health Insurance Research Database in Taiwan. Materials and Methods: A retrospective cohort study was conducted. Subjects were regarded as having PD according to the diagnostic codes. For comparison, each subject with PD was matched to one non-PD individual from the database after exclusion. The main outcome was defined as the development of infectious keratitis, endophthalmitis, orbital cellulitis, lacrimal duct infection, uveitis and infectious scleritis. Cox proportional hazard regression was used to yield the adjusted hazard ratios (aHR) of ocular diseases between the study and control groups. Results: A total of 426,594 subjects were enrolled in both the study and control groups. In the multivariable analysis, significantly higher rates of infectious keratitis (aHR: 1.094, 95% CI: 1.030&ndash
1.161), uveitis (aHR: 1.144, 95% CI: 1.074&ndash
1.218) and infectious scleritis (aHR: 1.270, 95% CI: 1.114&ndash
1.449) were found in the study group. Concerning the PD interval, infectious keratitis (aHR: 1.159, 95% CI: 1.041&ndash
1.291) and infectious scleritis (aHR: 1.345, 95% CI: 1.055&ndash
1.714) would significantly occur in PD patients with an interval shorter than two years, individuals with a PD interval that ranged from two to five years were under a higher risk of developing uveitis (aHR: 1.184, 95% CI: 1.065&ndash
1.315) and infectious scleritis (aHR: 1.386, 95% CI: 1.125&ndash
1.708), and the rate of uveitis (aHR: 1.149, 95% CI: 1.038&ndash
1.272) was significantly higher if PD persisted more than five years. Conclusions: The presence of PD will significantly elevate the risk of developing infectious keratitis, uveitis and infectious scleritis.

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ca7e3fa156c5fc53410abfb058b59d1
https://pubmed.ncbi.nlm.nih.gov/33218003

المؤلفون: Siu-Fung Chau, Pei-Hsuan Wu, Chi-Chin Sun, Jing-Yang Huang, Chan-Wei Nien, Shun-Fa Yang, Ming-Chih Chou, Pei-Ting Lu, Hung-Chi Chen, Chia-Yi Lee

المصدر: International Journal of Environmental Research and Public Health
Volume 16
Issue 22

مصطلحات موضوعية: Adult, Male, genetic structures, Health, Toxicology and Mutagenesis, Taiwan, Article, functional endoscopic sinus surgery, 03 medical and health sciences, 0302 clinical medicine, Risk Factors, otorhinolaryngologic diseases, Humans, Sinusitis, 030223 otorhinolaryngology, Aged, Retrospective Studies, Rhinitis, Aged, 80 and over, chronic rhinosinusitis, Public Health, Environmental and Occupational Health, Middle Aged, eye diseases, population-based, glaucoma, Chronic Disease, 030221 ophthalmology & optometry, Female

الوصف: This study investigates the development of glaucoma in subjects with surgery-indicated chronic rhinosinusitis (CRS) by the use of the National Health Insurance Research Database in Taiwan. Individuals that received the functional endoscopic sinus surgery (FESS) with a diagnostic code of CRS were regarded as surgery-indicated CRS and enrolled in the study group. Four non-CRS patients were age- and gender-matched to each patient in the study group. The exclusion criteria included legal blindness, ocular tumor, history of eyeball removal, and previous glaucoma. The outcome was regarded as the development of glaucoma, and conditional logistic regression was used for the statistical analysis, which involved multiple potential risk factors in the multivariate model. A total of 6506 patients with surgery-indicated CRS that received FESS and another 26,024 non-CRS individuals were enrolled after exclusion. The age and gender distributions were identical between the two groups due to matching. There were 108 and 294 glaucoma events in the study group and control group, respectively, during the follow-up period, and the study group had a significantly higher adjusted hazard ratio (1.291, 95% confidential interval: 1.031&ndash
1.615). The cumulative probability analysis also revealed a correlation between the occurrence of glaucoma and the CRS disease interval. In the subgroup analysis, the chance of developing open-angle glaucoma and normal-tension glaucoma was significantly higher in the study group than in the control group. In conclusion, the existence of surgery-indicated CRS is a significant risk factor for the development of glaucoma, which correlated with the disease interval.

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cb44eeede83433ad878eb500cecb62a

المؤلفون: Siu-Fung Chau, Sung-Ying Huang, Shu-Fang Chang, Sheng-Chun Chiu

المصدر: Biomolecules
Volume 9
Issue 8
Biomolecules, Vol 9, Iss 8, p 380 (2019)

مصطلحات موضوعية: 0301 basic medicine, Adult, Cell Survival, NF-E2-Related Factor 2, lcsh:QR1-502, hydrogen peroxide, Retinal Pigment Epithelium, medicine.disease_cause, Protective Agents, Biochemistry, lcsh:Microbiology, Article, Nrf2, Cell Line, 03 medical and health sciences, chemistry.chemical_compound, Structure-Activity Relationship, 0302 clinical medicine, hispidin, medicine, Humans, oxidative stress, Molecular Biology, age-related macular degeneration, biology, Dose-Response Relationship, Drug, Chemistry, GCLM, Transfection, biology.organism_classification, Cell biology, 030104 developmental biology, GCLC, Phellinus linteus, Pyrones, 030220 oncology & carcinogenesis, Hispidin, NAD+ kinase, ARPE-19, Oxidative stress, Intracellular, Signal Transduction

الوصف: Hispidin, a polyphenol compound isolated from Phellinus linteus, has been reported to possess antioxidant activities. In this study, we aimed to investigate the mechanisms underlying the protective effect of hispidin against hydrogen peroxide (H2O2)-induced oxidative stress on Adult Retinal Pigment Epithelial cell line-19 (ARPE-19) cells. Hispidin was not cytotoxic to ARPE-19 cells at concentrations of less than 50 &mu
M. The levels of intracellular reactive oxygen species (ROS) were analyzed by dichlorofluorescin diacetate (DCFDA) staining. Hispidin significantly restored H2O2-induced cell death and reduced the levels of intracellular ROS. The expression levels of antioxidant enzymes, such as NAD(P)H:Quinine oxidoreductase-1 (NQO-1), heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), and glutamate-cysteine ligase modifier subunit (GCLM) were examined using real-time PCR and Western blotting. Our results showed that hispidin markedly enhanced the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), HO-1, NQO-1, GCLM, and GCLC in a dose-dependent manner. Furthermore, knockdown experiments revealed that transfection with Nrf2 siRNA successfully suppresses the hispidin activated Nrf2 signaling in ARPE-19 cells. Moreover, activation of the c-Jun N-terminal kinase (JNK) pathway is involved in mediating the protective effects of hispidin on the ARPE-19 cells. Thus, the present study demonstrated that hispidin provides protection against H2O2-induced damage in ARPE-19 cells via activation of Nrf2 signaling and up-regulation of its downstream targets, including Phase II enzymes, which might be associated with the activation of the JNK pathway.

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1807fbcc21039aa64a72de16a9668fb8

المؤلفون: Chung-Yao Yang, Hin-Yeung Tsai, Keng-Hung Lin, Siu-Fung Chau, Chao-Min Cheng, Chun-Yuan Wang, Ying-Cheng Shen, Yu-Chen Chen, Wen-Hsin Hsu, Min-Yen Hsu

المصدر: Biomaterials. 35(12)

مصطلحات موضوعية: Paper, Vascular Endothelial Growth Factor A, medicine.medical_specialty, Pathology, Retinal Vein, Bevacizumab, Eye Diseases, VEGF receptors, Biophysics, Bioengineering, Aqueous humor, Enzyme-Linked Immunosorbent Assay, Biomaterials, Aqueous Humor, chemistry.chemical_compound, Limit of Detection, Ophthalmology, medicine, Humans, biology, business.industry, Retinal, Diabetic retinopathy, Macular degeneration, medicine.disease, Vascular endothelial growth factor, chemistry, Mechanics of Materials, Ceramics and Composites, biology.protein, business, medicine.drug

الوصف: The vascular endothelial growth factor (VEGF) level in aqueous humor has been used as an indicator to monitor specific diseases in the retinal ischemic condition. For clinical diagnosis, only about 200 μL of aqueous humor can be collected from the anterior chamber before the threat of anterior chamber collapse. It is necessary to develop an inexpensive diagnostic approach with the characteristics of highly sensitive, short operation duration, and requires small clinical sample quantities. To achieve the main objective of this study, we first prepared bevacizumab to be conjugated with HRP. We then deposited 2 μL aqueous humor from patients with different diseases onto each test zone of paper-based 96-well plates. After the colorimetric results were performed via ELISA protocol, the output signals were recorded using a commercial desktop scanner for analysis. In this study, only 2 μL from the aqueous humor of each patient was required for paper-based ELISA. The mean aqueous VEGF level was 14.4 pg/mL from thirteen patients (N = 13) with senile cataract as the control. However, the mean aqueous VEGF level from other patients with proliferative diabetic retinopathy (N = 14), age-related macular degeneration (N = 17), and retinal vein occlusion (N = 10) showed VEGF increases to 740.1 pg/mL, 383 pg/mL, and 219.4 pg/mL, respectively.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd154e5a6564e3227803c1cd29c919c7
https://pubmed.ncbi.nlm.nih.gov/24484673