المؤلفون: Tanemura, Nanae^{Aff1, IDs4200002400537y_cor1}, Kubota, Tetsuya^{Aff1, Aff2, Aff3, Aff4}, Kubota, Naoto^{Aff4, Aff5}, Chiba, Tsuyoshi

المصدر: Hormones: International Journal of Endocrinology and Metabolism. :1-10

المؤلفون: Venice Chávez Valencia, Victoriano Pérez-Vázquez, Anel Gómez García, Katya Vargas-Ortiz, Martha Arisbeth Villanueva Pérez, Marisol Godínez Rubí, Leonardo Pazarín Villaseñor, Sergio Gutiérrez Castellanos, Citlalli Orizaga de la Cruz

المصدر: Nefrología (English Edition), Vol 44, Iss 3, Pp 402-407 (2024)

مصطلحات موضوعية: Complement, Diagnosis, Focal segmental glomerulosclerosis, Surrogate marker, C4d staining, Diseases of the genitourinary system. Urology, RC870-923

الوصف: Background: There is a little information about of expression of C4d (complement fragment) in Focal segmental glomerulosclerosis (FSGS) subtypes. Our aim was to determine the expression of C4d in FSGS subtypes in percutaneous native renal biopsies in a second-level hospital and its correlation with clinical, biochemical and histological variables. Material and methods: A retrospective study in paraffin blocks of patients with biopsy with FSGS aged 16–65 years, indistinct sex, not diabetic or obese. Immunohistochemistry was performed for C4d and their expression was analyzing in non-sclerosed glomerular capillaries (GC) and sclerosis areas (SA). Clinical and biochemical variables were recorded. The cases were divided into C4d positive and C4d negative groups and compared. The correlation between C4d staining scores in CG and SA with clinical and biochemical variables were analyzed. Results: Twenty samples were analyzed, 4 for each subtype. At the time of biopsy average age 38.8 ± 18.6 years, 65% male, 8.7% were hypertension. The percentage of positivity for C4d was 40% in GC, 30% SA and 35% in mesangium. The highest expression was for cellular and collapsing subtypes. C4d positivity cases had increased proteinuria (p = 0.035). A significant correlation was found between percentage of C4d expression in CG with SA (p = 0.012) and SA with tubular atrophy and interstitial fibrosis (p

وصف الملف: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2013251424001196; https://doaj.org/toc/2013-2514

URL الوصول: https://doaj.org/article/74b3a0f6cbff415895ba40401937224d

المؤلفون: Venice Chávez Valencia, Victoriano Pérez-Vázquez, Anel Gómez García, Katya Vargas-Ortiz, Martha Arisbeth Villanueva Pérez, Marisol Godínez Rubí, Leonardo Pazarín Villaseñor, Sergio Gutiérrez Castellanos, Citlalli Orizaga de la Cruz

المصدر: Nefrología, Vol 44, Iss 3, Pp 402-407 (2024)

مصطلحات موضوعية: Complement, Diagnosis, Focal segmental glomerulosclerosis, Surrogate marker, C4d staining, Diseases of the genitourinary system. Urology, RC870-923

الوصف: Resumen: Antecedentes: Existe poca información acerca de la expresión del C4d (fragmento del complemento) en la glomeruloesclerosis focal y segmentaria (GEFS) y sus variantes. El objetivo del estudio fue determinar la expresión de C4d en las variantes de GEFS en biopsia renal percutánea de riñones nativos en un hospital de segundo nivel y su correlación con variables clínicas, bioquímicas e histológicas. Material y métodos: Estudio retrospectivo en bloques de parafina de pacientes con biopsia renal percutánea con GEFS de 16-65 años, sexo indistinto, no diabéticos ni obesos. Se realizó inmunohistoquímica para C4d, analizando su expresión en capilares glomerulares (CG) no esclerosados y áreas de esclerosis (EC). Se registraron variables clínicas y bioquímicas. Los casos fueron clasificados en C4d positivos o negativos y se compararon entre ellos. Se analizó la correlación entre las puntuaciones de C4d en CG y EC con variables clínicas y bioquímicas. Resultados: Se analizaron 20 muestras, 4 para cada variante. En el momento de la biopsia renal percutánea la edad fue de 38,8 ± 18,6 años, 65% sexo masculino, 8,7% hipertensos. El porcentaje de positividad para C4d fue del 40% en CG, del 30% en EC y del 35% en mesangio. La mayor expresión de C4d fue para las variantes celular y colapsante. Los casos C4d positivos tenían mayor proteinuria (p = 0,035). Se encontró correlación entre el porcentaje de expresión de C4d en CG y EC (p = 0,012) y de EC con atrofia tubular y fibrosis intersticial (p

وصف الملف: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S0211699523000668; https://doaj.org/toc/0211-6995

URL الوصول: https://doaj.org/article/89c9ea06bb8647b1b9e9d98529fcc5a8

المؤلفون: van Ewijk, Roelof^{Aff1, IDs0024702305745z_cor1}, Chatziantoniou, Cyrano^{Aff1, Aff2}, Adams, Madeleine, Bertolini, Patrizia, Bisogno, Gianni^{Aff5, Aff6}, Bouhamama, Amine, Caro-Dominguez, Pablo, Charon, Valerie, Coma, Ana, Dandis, Rana, Devalck, Christine, De Donno, Giulia, Ferrari, Andrea, Fiocco, Marta^{Aff1, Aff13}, Gallego, Soledad, Giraudo, Chiara, Glosli, Heidi, ter Horst, Simone A. J., Jenney, Meriel, Klein, Willemijn M., Leemans, Alexander, Leseur, Julie, Mandeville, Henry C., McHugh, Kieran, Merks, Johannes H. M.^{Aff1, Aff23}, Minard-Colin, Veronique, Moalla, Salma, Morosi, Carlo, Orbach, Daniel, Ording Muller, Lil-Sofie, Pace, Erika, Di Paolo, Pier Luigi, Perruccio, Katia, Quaglietta, Lucia, Renard, Marleen, van Rijn, Rick R., Ruggiero, Antonio, Sirvent, Sara I., De Luca, Alberto^{Aff2, Aff37}, Schoot, Reineke A.

المصدر: Pediatric Radiology. 53(12):2539-2551

المؤلفون: Cummings, Steven R, Kritchevsky, Stephen B

المصدر: GeroScience. 44(6)

مصطلحات موضوعية: Biomedical and Clinical Sciences, Clinical Sciences, Clinical Research, Aging, Clinical Trials and Supportive Activities, 8.4 Research design and methodologies (health services), Health and social care services research, Geroscience, Biomarkers, Outcome Assessment, Health Care, Biological Products, Biological age, Epigenetic age, Surrogate marker, Clinical trials, Genetics, Clinical sciences

الوصف: Treatments that target fundamental processes of aging are expected to delay several aging-related conditions simultaneously. Testing the efficacy of these treatments for potential anti-aging benefits will require clinical trials with endpoints that reflect the potential benefits of slowing processes of aging. There are several potential types of endpoints to capture the benefits of slowing a process of aging, and a consensus is needed to standardize and compare the results of these trials and to guide the analysis of observational data to support trial planning. Using biomarkers instead of clinical outcomes would substantially reduce the size and the duration of clinical trials. This requires validation of surrogate markers showing that treatment induced change in the marker reliably predicts the magnitude of change in the clinical outcome. The surrogate marker must also reflect the biological mechanism for the effect of treatment on the clinical outcome. "Biological age" is a superficially attractive marker for such trials. However, it is essential to establish that treatment induced change in biological age reliably predict the magnitude of benefits in the clinical outcome. Reaching consensus on clinical outcomes for geroscience trials and then validating potential surrogate biomarkers requires time, effort, and coordination that will be worthwhile to develop surrogate outcomes that can be trusted to efficiently test the value of many anti-aging treatments under development.

وصف الملف: application/pdf

URL الوصول: https://escholarship.org/uc/item/8wv9g0tr

المؤلفون: Li, Xinyi, Luo, Danju, Zhang, Liling, Li, Qiuhui, Fan, Jun, Zhang, Jiwei, Huang, Bo, Yang, Ming, Nie, Xiu, Chang, Xiaona^{Aff1, IDs1288502311513x_cor10}, Pan, Huaxiong^{Aff1, IDs1288502311513x_cor11}

المصدر: BMC Cancer. 23(1)

المؤلفون: Arushi Agrawal, Pooja Agrawal, Nupur Kaushik, Yatendra Mohan, Shikha Prakash, Akhil Pratap Singh, Ankur Agrawal

المصدر: National Journal of Laboratory Medicine, Vol 13, Iss 2, Pp PO27-PO30 (2024)

مصطلحات موضوعية: elisa, oral cancer, surrogate marker for oral cancer, Microbiology, QR1-502, Chemistry, QD1-999

الوصف: Introduction: Oral cancer presents challenging and unresolved problems for the human population, accounting for as much as 30-40% of all carcinomas in India. The current research focuses on the use of the tumour marker β2-microglobulin as a surrogate marker in patients with Oral Squamous Cell Carcinoma (OSCC) for early detection of cancer. Aim: To evaluate the level of serum β2-microglobulin in histologically diagnosed OSCC patients and compare it with age- and sex-matched healthy controls. Materials and Methods: This was a cross-sectional study conducted in the Department of Pathology at SN Medical College, Agra, over a period of one year and six months. The study included 50 histologically diagnosed OSCC cases and 40 age- and sex-matched healthy controls. Blood samples were taken from the healthy controls and OSCC patients, and the level of serum β2-microglobulin was measured using Enzyme Linked Immunosorbent Assay (ELISA). Statistical analysis was conducted using the Statistical Package for Social Sciences (SPSS) version 11. Z test and ANOVA test were used to compare various parameters. A p-value of

وصف الملف: electronic resource

Relation: https://www.njlm.net/articles/PDF/2837/66217_CE[Ra1]_F(KR)_QC(KK_RDW_SHU)_PF1(AP_SS)__PFA(SHU)_PB(AP_OM)_PN(SHU).pdf; https://doaj.org/toc/2277-8551; https://doaj.org/toc/2455-6882

URL الوصول: https://doaj.org/article/55cb777d5b4841f58475b000c171eacb

المؤلفون: Xinyi Li, Danju Luo, Liling Zhang, Qiuhui Li, Jun Fan, Jiwei Zhang, Bo Huang, Ming Yang, Xiu Nie, Xiaona Chang, Huaxiong Pan

المصدر: BMC Cancer, Vol 23, Iss 1, Pp 1-11 (2023)

مصطلحات موضوعية: Lymphoma, Large B-cell lymphoma, p53 immunohistochemistry, Surrogate marker, TP53 alterations, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background To clarify the relationship between p53 immunohistochemistry (IHC) staining and TP53 alterations (including mutations and deletions) in large B-cell lymphomas (LBCLs) and to explore the possibility of p53 IHC expression patterns as surrogate markers for TP53 alterations. Methods A total of 95 patients diagnosed with LBCLs were selected, and paraffin samples were taken for TP53 gene sequencing, fluorescence in situ hybridization and p53 IHC staining. The results were interpreted by experienced pathologists and molecular pathologists. Results Forty-three nonsynonymous TP53 mutations and p53 deletions were detected in 40 cases, whereas the remaining 55 cases had wild-type TP53 genes. The majority of TP53 mutations (34/43, 79.1%) occurred in exons 4-8, and R248Q was the most common mutation codon (4/43, 9.3%). The highest frequency single nucleotide variant was C > T (43.6%). p53 expression was interpreted as follows: Pattern A: p53 staining was positive in 0%-3% of tumor cells, Pattern B: p53 staining was positive in 4-65% of tumor cells, Pattern C: more than 65% of tumor cells were stained positive for p53. The p53 IHC expression patterns were associated with TP53 alterations. Gain of function variants and wild-type TP53 tended to exhibit type C and B p53 expression patterns, but loss of function variants were exclusively seen in type A cases. Additionally, interpretation of the staining by various observers produced significant reproducibility. Conclusions The p53 IHC expression patterns can be used to predict TP53 alterations and are reliable for diverse alteration types, making them possible surrogate biomarkers for TP53 alterations in LBCLs.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/1471-2407

URL الوصول: https://doaj.org/article/d20a12a6afaf4e46ad22bcd2530e214a

المؤلفون: Yuya Saito

المصدر: Exploration of Neuroprotective Therapy, Vol 3, Iss 4, Pp 186-206 (2023)

مصطلحات موضوعية: amyotrophic lateral sclerosis, surrogate marker, diffusion kurtosis imaging, diffusion tensor imaging, free water elimination diffusion tensor imaging, neurite orientation dispersion and density imaging, Neurology. Diseases of the nervous system, RC346-429

الوصف: Amyotrophic lateral sclerosis (ALS) is the most prevalent type of motor neuron disease (MND) and is diagnosed with a delay from the first appearance of symptoms. Surrogate markers that may be used to detect pathological changes before a significant neuronal loss occurs and allow for early intervention with disease-modifying therapy techniques are desperately needed. Using water molecules that diffuse within the tissue and experience displacement on the micron scale, diffusion magnetic resonance imaging (MRI) is a promising technique that can be used to infer microstructural characteristics of the brain, such as microstructural integrity and complexity, axonal density, order, and myelination. Diffusion tensor imaging (DTI) is the primary diffusion MRI technique used to evaluate the pathogenesis of ALS. Neurite orientation dispersion and density imaging (NODDI), diffusion kurtosis imaging (DKI), and free water elimination DTI (FWE-DTI) are only a few of the approaches that have been developed to overcome the shortcomings of the diffusion tensor technique. This article provides a summary of these methods and their potential as surrogate markers for detecting the onset of ALS at an early stage.

وصف الملف: electronic resource

Relation: https://www.explorationpub.com/Journals/ent/Article/100447; https://doaj.org/toc/2769-6510

URL الوصول: https://doaj.org/article/2c2bab68138e4817a9d4d02577fd1b5f

المؤلفون: Ichiro Yoshii, Naoya Sawada, Tatsumi Chijiwa

المصدر: Journal of General and Family Medicine, Vol 24, Iss 3, Pp 178-184 (2023)

مصطلحات موضوعية: creatinine, cystatin C, fracture, osteoporosis, risk ratio, surrogate marker, Medicine (General), R5-920

الوصف: Abstract Background Detection of appropriate indicators is valuable for preventing incidental osteoporotic fractures. We statistically evaluated the significance of serum cystatin C‐to‐creatinine ratio (CysC/Cr) as a surrogate marker for incident major osteoporotic fractures (MOF) prediction. Methods Eligible patients with simultaneous measurement of CysC/Cr and bone mineral density in the lumbar spine and proximal femur were selected, and their fracture histories until 5 years after baseline were observed in the retrospective area cohort data. Patients who were followed up until termination or the first osteoporotic fracture were included, and loss of follow‐up or death was excluded. Candidate risk factors for osteoporotic fractures were tested for risk ratios using a cox regression analysis. Receiver operating characteristic tests were performed on factors with significantly higher risk ratios and evaluated with Kaplan‐Meier survival analysis to determine the hazard ratios of the factors. Results A total of 175 patients of whom 28 had incident MOF, 38 men, and 137 women, were enrolled. The mean age was 70.2 years. A significantly higher risk ratio was shown in the presence of prevalent MOF, hyper fall‐ability, lifestyle‐related diseases, chronic kidney diseases ≥ Grade3a, and higher CysC/Cr. All parameters had cutoff indices and showed significantly higher hazard ratios. Conclusions These results suggested that CysC/Cr may be a predictive marker of incident osteoporotic fractures. It might work as a screening tool for MOF risk.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2189-7948

URL الوصول: https://doaj.org/article/a4dfc84cdf714c55828fbaf7276c9d35