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11
المؤلفون: Yong-hao Yu, Qiang Fu, Jun Li, Nai-qiang Cui
المصدر: Chinese Journal of Integrative Medicine. 11:136-141
مصطلحات موضوعية: Male, medicine.medical_specialty, medicine.medical_treatment, Intraperitoneal injection, Peritonitis, Inflammation, Severity of Illness Index, Proinflammatory cytokine, Rats, Sprague-Dawley, Interferon-gamma, Th2 Cells, Sepsis, Internal medicine, medicine, Animals, Pharmacology (medical), Interferon gamma, Survival rate, Plant Extracts, Tumor Necrosis Factor-alpha, business.industry, Interleukin, General Medicine, Th1 Cells, medicine.disease, Interleukin-12, Recombinant Proteins, Interleukin-10, Rats, Survival Rate, Disease Models, Animal, Drug Combinations, Endocrinology, Complementary and alternative medicine, Immunology, Cytokines, Drug Therapy, Combination, Tumor necrosis factor alpha, medicine.symptom, business, Drugs, Chinese Herbal, medicine.drug
الوصف: Objective: To evaluate the dynamic change of Thl/Th2 cytokines in serum, peritoneal lavage fluid (PLF) and splenic macrophages (SM) in rats with severe peritonitis, and to observe the therapeutic effects of recombinant interleukin-12 (rlL-2) and Shenmai injection (SMI), a Chinese medicinal preparation.Methods: Severe peritonitis (SP) model was induced by intraperitoneal injection of E. coli and B. frag, and mild peritonitis (MP) model was induced by cecal ligation and punching. Then the following experiments were done: (1) Survival rates of animals after every 6 hrs in the 72 hrs after modeling were recorded, serum and PLF levels of cytokines, including tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and interleukin-10 (IL-10), 6 hrs, 12 hrs, 24 hrs and 48 hrs after modeling were measured. (2) Model rats were treated with rIL-12 or SMI, the survival rate was recorded and serum levels of TNF-α, INF-γ, and IL-10 before and after treatment were measured, and (3) amount of these cytokines produced by SM were determined 6 hrs, 12 hrs and 24 hrs after treatment. The survival rates and levels of cytokines were then compared between the groups (model group treated with rIL-12 or SMI, untreated model group, and blank group).Results: Serum and PLF levels of IFN-γ, TNF-α at all the time points in SP rats were significantly lower than those in MP rats while those of IL-10 6 hrs and 12 hrs after modeling were significantly higher in the former than that in the latter (P
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8100a44753818e23ea5a2aa317fcab3
https://doi.org/10.1007/bf02836471 -
12
المؤلفون: Dilpesh Jain, Rahul Somani, Rahul Dalvi, Aman Upganlawar, Manish Kumar Bansal
المصدر: Journal of integrative medicine. 12(1)
مصطلحات موضوعية: Blood Glucose, Male, medicine.medical_specialty, Citrus, Diabetic neuropathy, medicine.medical_treatment, Intraperitoneal injection, Diosmin, Type 2 diabetes, Rats, Sprague-Dawley, chemistry.chemical_compound, Insulin resistance, Diabetic Neuropathies, Internal medicine, Diabetes mellitus, Malondialdehyde, Medicine, Animals, Humans, business.industry, Plant Extracts, General Medicine, medicine.disease, Streptozotocin, Glutathione, Rats, Oxidative Stress, Endocrinology, Cholesterol, chemistry, business, medicine.drug
الوصف: Objective The present study was undertaken to evaluate the effect of diosmin in diabetic neuropathy in type 2 diabetic rats. Methods Type 2 diabetes was induced in male Sprague-Dawley rats by single intraperitoneal injection of streptozotocin (35 mg/kg) and high-fat diet. Four weeks after the confirmation of diabetes, diabetic rats were treated with diosmin (50 and 100 mg/kg, p.o.) for next 4 weeks. Rats were evaluated for biochemical, behavioral and oxidative stress parameters. Eddy's hot plate and tail immersion test were performed on 6th, 7th, 8th, 9th and 10th weeks of experiment to assess thermal hyperalgesia and cold allodynia respectively. Further, the walking function test was performed for assessing the motor responses at the end of the treatment schedule. Results Rats were fed with high-fat diet throughout the experiment schedule and administration of low-dose streptozotocin induced significant elevation in blood glucose level and insulin resistance which was confirmed by oral glucose tolerance test. Treatment with diosmin at doses of 50 and 100 mg/kg significantly restored the reduced body weight, elevated blood sugar and lipid profiles. Further the dose-dependent improvement was observed in thermal hyperalgesia, cold allodynia and walking function in diabetic rats treated with diosmin. Elevated levels of malondialdehyde, and nitric oxide and decreased glutathione levels and superoxide dismutase activity in diabetic rats were restored significantly after the 4 weeks of diosmin treatment. Conclusion Diosmin has shown beneficial effect in preventing the progression of early diabetic neuropathy in rats.
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