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المؤلفون: Wenyi Guo, Chen-yang Wang, Weixing Wang, Liang Zhao, Wenhong Deng, Chen Chen, Fangchao Mei, Yupu Hong, Jia Yu, Qiao Shi, Yu Zhou
المصدر: Biochemical and biophysical research communications. 505(3)
مصطلحات موضوعية: Taurocholic Acid, medicine.medical_specialty, Thyroid Hormones, Biophysics, Thyroid Gland, Inflammation, Biochemistry, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Microscopy, Electron, Transmission, Pregnancy, Internal medicine, medicine, Animals, Humans, Molecular Biology, Pancreas, business.industry, CD68, Thyroid, Cell Biology, medicine.disease, Pregnancy Complications, Disease Models, Animal, medicine.anatomical_structure, Endocrinology, Pancreatitis, 030220 oncology & carcinogenesis, Acute Disease, Amylases, Acute pancreatitis, Cytokines, 030211 gastroenterology & hepatology, Female, medicine.symptom, Pancreatic injury, business
الوصف: Acute pancreatitis in pregnancy (APIP), which was thought to be a rare but severe disease, with a high perinatal mortality among maternal-fetuses. Our research aimed to study and assess thyroid injury in a rat model of APIP and its possible mechanisms. The APIP model was established by retrograde injection with sodium taurocholate. Sham-operated (SO) and APIP groups were performed at 3 time-points. Histological changes in the maternal thyroid and pancreas were assessed. The activities of serum amylase, lipase and levels of FT3, FT4, MDA, TNF-α and IL-1β were detected in maternal rats, and the expression of MIF, ICAM-1 and CD68 in the maternal thyroids were determined. In this study, maternal thyroid injury as well as pancreas injury occurred in a time-dependent manner. The activities of serum amylase, lipase and levels of MDA, TNF-α and IL-1β were markedly increased in acute pancreatitis rats, the levels of serum FT3 and FT4 were obviously decreased in APIP groups, and the expressions of MIF, ICAM-1 and CD68 were significantly increased in the thyroid of the APIP group. Ultrastructural thyroid injuries were observed in the APIP group. Our research suggests that thyroid injury is involved in the rat experimental model of APIP. The degree of thyroid dysfunction is associated with APIP, which may affect the prognosis of acute pancreatitis.
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المؤلفون: Kailiang Zhao, Yupu Hong, Wenyi Guo, Jia Yu, Ying Chen, Chen-yang Wang, Qiao Shi, Weixing Wang, Xiao-yi Zhang, Liang Zhao, Chen Chen, Fangchao Mei, Yu Zhou
المصدر: International Immunopharmacology. 87:106771
مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, medicine.medical_treatment, Immunology, Thyroid Gland, Protective Agents, medicine.disease_cause, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Pregnancy, Internal medicine, medicine, Animals, Immunology and Allergy, Macrophage Migration-Inhibitory Factors, Pancreas, Pharmacology, business.industry, Thyroid, Interleukin, Isoxazoles, medicine.disease, Intramolecular Oxidoreductases, 030104 developmental biology, medicine.anatomical_structure, Cytokine, Endocrinology, Pancreatitis, 030220 oncology & carcinogenesis, Cytokines, Acute pancreatitis, Female, Macrophage migration inhibitory factor, Tumor necrosis factor alpha, business, Oxidative stress
الوصف: Acute pancreatitis during pregnancy (APIP) rarely occurs but may lead to preterm delivery and be associated with high fetal mortality. Macrophage migration inhibitory factor (MIF) participates in various inflammatory diseases as a pro-inflammatory cytokine. In this study, we aimed to explore the effects of (S, R)-3-(4-hydroxyphenyl)-4, 5dihydro-5-isoxazole acetic methyl ester (ISO-1), an inhibitor of MIF, on maternal thyroid injury associated with APIP and its potential mechanisms in a pregnant rat model. APIP model was induced by retrograde injection of sodium taurocholate. ISO-1 was injected intraperitoneally 30 min before model establishment. The severity of pancreatitis was assessed by levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, IL-6 of maternal serum as well as histopathological score. Thyroid injury was determined by free triiodothyronine (FT3), free tetraiodothyronine (FT4) and thyroid histopathological score. Levels of MIF in maternal serum and the expression of MIF, CD68, CD3 and intercellular cell adhesion molecule-1 (ICAM-1) as well as oxidative stress status in maternal thyroid tissues were detected. Ultrastructure of maternal thyroid tissues was observed by transmission electron microscope. Thyroid injuries occurred in APIP and the lesions were attenuated with the pretreatment of ISO-1. Moreover, ISO-1 reduced the expression of MIF, attenuated the activations of CD68, CD3, ICAM-1 while improved oxidative stress status in maternal thyroid. Our research suggested a protective role of ISO-1 on thyroid injury and endocrine disorder during APIP, which may be associated with the inhibition of biological functions of MIF.
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