المؤلفون: Afridi, Mahideen, Ahmad, Khurshid, Malik, Shahana Seher, Rehman, Nazia, Yasin, Muhammad, Khan, Shujaul Mulk, Hussain, Adil, Khan, Muhammad Ramzan

المصدر: Journal of Genetic Engineering & Biotechnology; 9/26/2022, Vol. 20 Issue 1, p1-12, 12p

مصطلحات موضوعية: MUSTARD, BRASSICACEAE, RAPESEED, PHYLOGENY, GENES, CULTIVARS

مستخلص: Background: Non-synchronized pods shattering in the Brassicaceae family bring upon huge yield losses around the world. The shattering process was validated to be controlled by eight genes in Arabidopsis, including SHP1, SHP2, FUL, IND, ALC, NAC, RPL, and PG. We performed genome-wide identification, characterization, and expression analysis of shattering genes in B.napus and B. juncea to gain understanding into this gene family and to explain their expression patterns in fresh and mature siliques. Results: A comprehensive genome investigation of B.napus and B.juncea revealed 32 shattering genes, which were identified and categorized using protein motif structure, exon-intron organization, and phylogeny. The phylogenetic study revealed that these shattering genes contain little duplications, determined with a distinct chromosome number. Motifs of 32 shattering proteins were observed where motifs1 and 2 were found to be more conserved. A single motif was observed for other genes like Br-nS7, Br-nS9, Br-nS10, Br-jS21, Br-jS23, Br-jS24, Br-jS25, and Br-jS26. Synteny analysis was performed that validated a conserved pattern of blocks among these cultivars. RT-PCR based expressions profiles showed higher expression of shattering genes in B. juncea as compared to B.napus. SHP1, SHP2, and FUL gene were expressed more in mature silique. ALC gene was upregulated in fresh silique of B. napus but downregulation of ALC were observed in fresh silique of B. juncea. Conclusion: This study authenticates the presence of shattering genes in the local cultivars of Brassica. It has been validated that the expression of shattering genes were more in B. juncea as compared to B.napus. The outcomes of this study contribute to the screening of more candidate genes for further investigation. [ABSTRACT FROM AUTHOR]

: Copyright of Journal of Genetic Engineering & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Afridi, Mahideen, Ahmad, Khurshid, Malik, Shahana Seher, Rehman, Nazia, Yasin, Muhammad, Khan, Shujaul Mulk, Hussain, Adil, Khan, Muhammad Ramzan

المصدر: Journal of Genetic Engineering & Biotechnology; 8/18/2022, Vol. 20 Issue 1, p1-12, 12p

مصطلحات موضوعية: MUSTARD, BRASSICACEAE, RAPESEED, PHYLOGENY, GENES, CULTIVARS

مستخلص: Background: Non-synchronized pods shattering in the Brassicaceae family bring upon huge yield losses around the world. The shattering process was validated to be controlled by eight genes in Arabidopsis, including SHP1, SHP2, FUL, IND, ALC, NAC, RPL, and PG. We performed genome-wide identification, characterization, and expression analysis of shattering genes in B.napus and B. juncea to gain understanding into this gene family and to explain their expression patterns in fresh and mature siliques. Results: A comprehensive genome investigation of B.napus and B.juncea revealed 32 shattering genes, which were identified and categorized using protein motif structure, exon-intron organization, and phylogeny. The phylogenetic study revealed that these shattering genes contain little duplications, determined with a distinct chromosome number. Motifs of 32 shattering proteins were observed where motifs1 and 2 were found to be more conserved. A single motif was observed for other genes like Br-nS7, Br-nS9, Br-nS10, Br-jS21, Br-jS23, Br-jS24, Br-jS25, and Br-jS26. Synteny analysis was performed that validated a conserved pattern of blocks among these cultivars. RT-PCR based expressions profiles showed higher expression of shattering genes in B. juncea as compared to B.napus. SHP1, SHP2, and FUL gene were expressed more in mature silique. ALC gene was upregulated in fresh silique of B. napus but downregulation of ALC were observed in fresh silique of B. juncea. Conclusion: This study authenticates the presence of shattering genes in the local cultivars of Brassica. It has been validated that the expression of shattering genes were more in B. juncea as compared to B.napus. The outcomes of this study contribute to the screening of more candidate genes for further investigation. [ABSTRACT FROM AUTHOR]

: Copyright of Journal of Genetic Engineering & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Afridi, Mahideen, Ahmad, Khurshid, Malik, Shahana Seher, Rehman, Nazia, Yasin, Muhammad, Khan, Shujaul Mulk, Hussain, Adil, Khan, Muhammad Ramzan

المصدر: Journal of Genetic Engineering & Biotechnology; 9/26/2022, Vol. 20 Issue 1, p1-1, 1p

مصطلحات موضوعية: MUSTARD, BRASSICACEAE, RAPESEED, PHYLOGENY, GENES