المؤلفون: WU, L.-L., JIA, Y.-M., SUI, Y.-X., AO, S., WANG, Y., LIU, Y., XU, W.-D., ZHANG, H.-B., ZHANG, H., MAO, J.-H., YANG, X.-T., LENG, H.

المصدر: European Review for Medical & Pharmacological Sciences; 2022, Vol. 26 Issue 15, p5413-5421, 9p

مستخلص: OBJECTIVE: We explored the influences on platelet-rich fibrin (PRF) to rat Bone Mesenchymal Stem Cells (BMSCs), as well as the role of bone morphogenetic protein 2 (BMP2)/maternal signal protein homolog (Smads) pathway. MATERIALS AND METHODS: The proposed research is approved by the ethics board of the Second Affiliated Hospital of Harbin Medical University. The BMSCs were isolated and purified. The BMSCs were assigned to a control group arbitrarily, PRF group, BMP activator group and BMP inhibitor group (hereinafter referred to as activator group and inhibitor group). Each group of BMSCs in the logarithmic growth phase was detected for the alkaline phosphatase (ALP) activity since 3 days and 14 days of culture; CCK-8 assay was conducted for detection of the proliferation of BMSCs; Real time PCR was conducted for detection of the osteogenic differentiation marker collagen I (COL-I), BMP2, Runt-related transcription factor 2(RUNX2), osteocalcin (OCN) mRNA relative expression levels; Western-Blot detection of BMP2, OCN, P-SMAD1/5/8, relative expression level of RUNX2 protein. RESULTS: In contrast to the control group, BMSCs' the ALP activity of the PRF group, activator group, as well as inhibitor group increased for 3 days and 14 days, and the activator group>PRF group>inhibitor group (p=0.05). ALP activity in each group was elevated with the increase in culture time, the ALP activity of the control group, PRF group, activator group and inhibitor group increased (p=0.05). In comparison to the control group, the relevant expression levels of COL-I, BMP-2, RUNX2 and OCN in the PRF group, activator group, and inhibitor group increased, and the activator group>PRF group>inhibitor group (p=0.05). The relative expression levels of BMP2, OCN, p-SMAD1/5/8 and RUNX2 protein in each group were statistically different, the activator group>PRF group>control group>inhibitor group (p=0.05). CONCLUSIONS: PRF can promote the proliferation and osteogenic differentiation of BMSCs by activating the BMP2/Smads signaling pathway. [ABSTRACT FROM AUTHOR]

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المؤلفون: WEI, S.-J., LI, J., WANG, J.-Y., ZHANG, L.-X., ZHANG, H.-B., DU, D.-J., LI, Q.-X.

المصدر: European Review for Medical & Pharmacological Sciences; May2022, Vol. 26 Issue 9, p3074-3082, 9p

مستخلص: OBJECTIVE: To elucidate the biological function of BAP18 (BPTF-associated protein of 18 kDa) in non-small-cell lung carcinoma (NSCLC) and the molecular mechanism. PATIENTS AND METHODS: Relative levels of BAP18 in NSCLC tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR), and its influence on pathological characteristics of NSCLC patients was analyzed. Correlation between BAP18 and Ki67 levels in NSCLC was assessed by Pearson correlation test. Furthermore, Kaplan-Meier curves were depicted for revealing survival difference in NSCLC patients expressing high or low level of BAP18. Relative levels of BAP18, CCND1, CCND2 and CCND3 in A549 and H1299 cells transfected with siBAP18 were determined, as well as colony number. In addition, after knockdown of protein level of BAP18 in A549 and H1299 cells by lentivirus transfection, cell cycle progression was examined. Co-regulation of BAP18 and CCND1/2 on cell growth of NSCLC was finally detected. RESULTS: BAP18 was upregulated in NSCLC tissues, especially cases with advanced stage (IIIIV) or large tumor size (>5 cm). BAP18 was closely linked to tumor size, TNM staging and lymphatic metastasis in NSCLC. Knockdown of BAP18 reduced transcriptional levels of CCND1 and CCND2 in A549 and H1299 cells. Furthermore, knockdown of BAP18 delayed transition from G1 to S phase, and weakened growth of NSCLC cells. CONCLUSIONS: BAP18 triggers the progression of NSCLC by regulating transcriptional activities of CCND1/2, which may be a potential target for the treatment and diagnosis of NSCLC. [ABSTRACT FROM AUTHOR]

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المؤلفون: XU, N., CHEN, Y.-S., ZHU, H., LI, W.-S., SUN, Z.-W., ZHANG, H.-B., LI, X.-Y., ZHANG, B., ZHANG, C., WEN, Z.-Q., BAI, Y.-T.

المصدر: European Review for Medical & Pharmacological Sciences; 2021, Vol. 25 Issue 16, p5293-5303, 11p

مستخلص: OBJECTIVE: This study was probed to uncover the mechanism of miR-142-5p in septic liver injury. MATERIALS AND METHODS: In this study, in-vitro and in-vivo models of sepsis were used. For in-vitro sepsis model, hepatocyte cell line (L02 cells) was treated with LPS (lipopolysaccharide). Whereas for in-vivo sepsis model, cecal ligation and puncture were performed in mice. Mice were assigned into three groups: control, CLP (Cecal Ligation Puncture), CLP + miR-142- 5p inhibitor group. Liver injury was assessed via H&E staining. IL-6, TNF-a, and IL-1ß expressions were assayed through ELISA kits. C-caspase-9, C-caspase-3, ERK, p65, and IκBa expressions were determined via western blot and RT-qPCR. Apoptosis in LPS-induced L02 cells was detected by TUNEL staining. RESULTS: Our results show that miR-142-5p exhibited perspicuous upregulation in CLP mice tissues and LPS-induced L02 cells. On the other hand, inhibition of miR-142-5p could promote LPS-induced L02 cell activity and reduce apoptosis and inflammation. In terms of molecular mechanism, downregulation of miR-142-5p could abate sepsis-mediated acute hepatic injury by targeting SOCS1, through ERK and NF-κB pathway. CONCLUSIONS: Overall our results demonstrate that miR-142-5p inhibitors can mitigate septic liver injury by downregulating the inflammation and apoptosis via targeting SOCS1. Thus, miR-142-5p can serve a potential therapeutic target for sepsis mediated acute hepatic injury. [ABSTRACT FROM AUTHOR]

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المؤلفون: ZHANG, H.-B., SHEN, B., MA, Z.-C., XU, Y.-Y., LOU, Y.-L., CHEN, M.

المصدر: European Review for Medical & Pharmacological Sciences; 2020, Vol. 24 Issue 8, p4298-4305, 8p

مستخلص: OBJECTIVE: The aim of this study was to investigate the role of microRNA- 593-5p (miR-593-5p) in the development of lung adenocarcinoma (LA). PATIENTS AND METHODS: The expression level of miR-593-5p in LA tissues and cell lines was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Luciferase reporter gene assay and Western blot were performed to evaluate the interaction between miR- 593-5p and intercellular cell adhesion molecule- 1 (ICAM-1). Furthermore, the effects of the miR-593-5p/ICAM-1 axis on A549 cells were determined by MTS, colony formation assay, and transwell assay, respectively. RESULTS: MiR-593-5p was significantly downregulated in both clinical samples and cell lines. The bioinformatics analysis predicted that miR- 593-5p could complementarily bind to the 3'-UTR of ICAM-1. Luciferase reporter gene assay confirmed that ICAM-1 was the direct target of miR- 593-5p. Western blot results demonstrated that miR-593-5p could effectively reduce the protein expression of ICAM-1 in cells. In vitro experiments indicated that the proliferation and migration of A549 cells were significantly inhibited by miR-593- 5p transfection. However, the overexpression of ICAM-1 could effectively reverse the inhibitory effects of miR-593-5p in vitro. These results indicated that the inhibitory effects of miR-593-5p on LA were achieved by regulating ICAM-1 expression. CONCLUSIONS: MiR-593-5p/ICAM-1 axis might be a potential therapeutic target for the diagnosis and treatment of LA. [ABSTRACT FROM AUTHOR]

: Copyright of European Review for Medical & Pharmacological Sciences is the property of Verduci Editore and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: WU, T. -Y., ZHANG, H. -B.

المصدر: European Review for Medical & Pharmacological Sciences; 2019 Supplement3, Vol. 23, p184-191, 8p

مستخلص: OBJECTIVE: To study the protective effect of glutamine on the intestinal tissues of septic rats by regulating the nuclear factor-κB (NF-κB) pathway. MATERIALS AND METHODS: A total of 30 rats were divided into the Sham group, Model group, and Glutamine group using a random number table. The changes in the intestinal tissues in rats were observed via hematoxylin-eosin (HE) staining, and the difference in the content of serum inflammatory factor tumor necrosis factor-α (TNF-α) was detected via enzyme-linked immunosorbent assay (ELISA). Moreover, the apoptosis of the intestinal tissues was detected via terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, and the protein expression of NF-κB in intestinal tissues was detected via Western blotting. RESULTS: In the Sham group, the rats had normal activity and good mental state, and there were no evident lesions in the abdominal cavity. Compared with the rats in the Sham group, the rats in the Model group had very poor mental state and erected hair, and they trembled and barely moved. After the abdomen was opened, there were bad smell and evident bleeding in the abdominal cavity, and the cecum became black with adhesion and swelling. In the Glutamine group, the symptoms were significantly alleviated compared with the Model group. The morphological observation of the intestinal tissues revealed that in the Sham group, the intestinal villi were regularly and clearly arranged, and there was no congestion in the capillaries. Compared with the Sham group, the intestinal villi were disorderly arranged with rupture in the Model group, and the severe capillary congestion was clearly visible and accompanied by ulcer. In the Glutamine group, the intestinal villi had normal morphology and regular arrangement after treatment, the subepithelial space was significantly dilated, the capillary dilation and the congestion could be seen and the lamina propria was intact. In the Sham group, the pathological score was 0 point, and the intestinal mucosa and villi had normal structure. Compared with that in the Sham group, the pathological score of the intestinal tissues were significantly increased in the Model group (p<0.05). In the Glutamine group, the pathological score significantly declined after treatment compared with that in the Model group (p<0.05). Besides, the content of the inflammatory factor TNF-α in the intestinal tissues was the highest in the Model group (p<0.05), and it was lower in the Glutamine group than that in the Sham group (p<0.05), indicating that glutamine can effectively reduce the content of the inflammatory factor TNF-α, exerting a certain protective effect on the intestinal tissues. The number of apoptotic intestinal epithelial cells was remarkably increased in the Model group compared with that in the Sham group (p<0.05), and it was remarkably decreased in the Glutamine group compared with that in the Model group (p<0.05). The Model group had a significantly higher protein expression of NF-κB in intestinal tissues than in the Sham group and Glutamine group (p<0.05), Sham group had the lowest protein expression of NF-κB in intestinal tissues (p<0.05), and the Glutamine group had a significantly lower protein expression of NF-κB in intestinal tissues than the Model group (p<0.05). CONCLUSIONS: Glutamine inhibits the protein expression of NF-κB, thereby exerting a protective effect on intestinal tissues of sepsis rats. [ABSTRACT FROM AUTHOR]

: Copyright of European Review for Medical & Pharmacological Sciences is the property of Verduci Editore and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: HU, Y.-L., WANG, H., HUANG, Q., WANG, G., ZHANG, H.-B.

المصدر: European Review for Medical & Pharmacological Sciences; 2018, Vol. 22 Issue 9, p2809-2816, 8p

مستخلص: OBJECTIVE: To explore the relationship between microRNA-23a-3p expression and perihematomal edema (PHE) in patients with acute intracerebral hemorrhage (ICH) and its underlying mechanism. PATIENTS AND METHODS: Clinical data and blood samples on the 3rd day after ICH onset were collected. Head CT was performed in each subject when admitted. Serum expressions of microRNAs were detected by quantitative real-time PCR (qRT-PCR). The relationship between hematoma volume and perihematomal edema was analyzed by correlation analysis. The direct binding of microRNA-23a-3p and zonula occludens-1 (ZO-1) was verified by luciferase activity assay and Western blot, respectively. Moreover, in vitro experiments were carried out by flow cytometry and CCK-8 assay, respectively. RESULTS: Serum levels of microRNA-23a-3p and microRNA-130a in ICH patients were remarkably higher than those in the control group, microRNA-26a and microRNA-146a, however, were significantly decreased. A positive correlation was observed between the microRNA-23a-3p expression and the volume of relative perihematomal edema (rPHE) on the 3rd day after ICH (r2=0.3985; p=0.0002). Up-regulation of microRNA-23a-3p significantly decreased ZO1 expression in hCMEC/D3 cells. Results of luciferase activity assay further indicated that microRNA-23a-3p directly targets the wild-type of ZO-1. In vitro results suggested that microRNA-23a-3p expression markedly affects the proliferation and apoptosis of hCMEC/D3 cells. Similar results were obtained after overexpression or knockdown of ZO-1. CONCLUSIONS: Up-regulated microRNA-23a-3p in ICH patients promotes the apoptosis of cerebral vascular endothelial cells by down-regulatingZO-1, thus participating in the perihematomal edema formation after intracerebral hematoma. [ABSTRACT FROM AUTHOR]

: Copyright of European Review for Medical & Pharmacological Sciences is the property of Verduci Editore and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Nie, X. J., Zhang, H. B., Li, S. Y.

المصدر: Soil Research; 2019, Vol. 57 Issue: 5 p513-519, 7p

مستخلص: Little is known about the effect of field management on soil quality in mining subsidence landscapes. In this study, we selected subsided cropland from the Jiaozuo coal mining district, China, to determine the effects of irrigation and tillage on soil organic carbon (SOC) and nutrients. Irrigation and tillage differentially affected the dynamics of SOC, total nitrogen (N) and total phosphorus (P) in subsided cropland at 15–18 years after surface subsidence. Tillage along a longitudinal slope-direction induced greater depletions of SOC, N and P stocks in subsided cropland compared with tillage along a transverse slope-direction (TT). These parameters were also more depleted under rainfed than under irrigated conditions. The distribution of SOC, total N and total P was related to soil redistribution by soil erosion in subsided cropland. Integrated irrigation-TT management reduced the within-field variations in SOC, total N and total P, and irrigation reduced within-field variation in SOC. These results indicate the effects of tillage and irrigation on soil erosion and subsequently on SOC and nutrients in subsided cropland. Practicing tillage over great subsidence gradients is detrimental to soil conservation. Combining small subsidence gradients with irrigation may be an alternative to traditional engineering reclamation of subsided cropland.

المؤلفون: Zhao, Y., Liu, J. C., Xiong, S. L., Xue, W. C., Yi, Q. B., Lu, G. P., Xu, W., Lyu, F. C., Sun, J. C., Peng, W. X., Zheng, C., Zhang, Y. Q., Cai, C., Xiao, S., Xie, S. L., Wang, C. W., Tan, W. J., An, Z. H., Chen, G., Du, Y. Q., Huang, Y., Gao, M., Gong, K., Guo, D. Y., He, J. J., Li, B., Li, G., Li, X. Q., Li, X. B., Liao, J. Y., Liang, J., Liang, X. H., Liu, Y. Q., Ma, X., Qiao, R., Song, L. M., Song, X. Y., Sun, X. L., Wang, J., Wang, J. Z., Wang, P., Wen, X. Y., Wu, H., Xu, Y. B., Yang, S., Zhang, B. X., Zhang, D. L., Zhang, F., Zhang, P., Zhang, H. M., Zhang, Z., Zhao, X. Y., Zheng, S. J., Zhang, K. K., Han, X. B., Wu, H. Y., Hu, T., Geng, H., Zhang, H. B., Lu, F. J., Zhang, S. N., Yu, H.

المصدر: Geophysical Research Letters; July 2023, Vol. 50 Issue: 14

مستخلص: Gravitational‐wave high‐energy Electromagnetic Counterpart All‐sky Monitor (GECAM) is a space‐borne instrument dedicated to monitoring high‐energy transients, including Terrestrial Gamma‐ray Flashes (TGFs) and Terrestrial Electron Beams (TEBs). We implemented a TGF/TEB search algorithm for GECAM, with which 147 bright TGFs, 2 typical TEBs and 2 special TEB‐like events are identified during an effective observation time of ∼9 months. We show that, with gamma‐ray and charged particle detectors, GECAM can effectively identify and distinguish TGFs and TEBs, and measure their temporal and spectral properties in detail. A very high TGF‐lightning association rate of ∼80% is obtained between GECAM and GLD360 in east Asia region. Terrestrial gamma‐ray flashes (TGFs) and Terrestrial Electron Beams (TEBs) represent the most energetic radioactive phenomena in the atmosphere of the Earth. They reflect a natural particle accelerator that can boost electrons up to at least several tens of mega electron volts and produce gamma‐ray radiation. With novel detection technologies, Gravitational‐wave high‐energy Electromagnetic Counterpart All‐sky Monitor (GECAM) is a new powerful instrument to observe TGFs and TEBs, as well as study their properties. For example, it is difficult for most space‐borne high‐energy instruments to distinguish between TGFs and TEBs. However, we show here that, with the joint observation of gamma‐ray and charged particle detectors, GECAM can effectively identify TGFs and TEBs. GECAM can also reveal their fine features in the light curves and spectra. During 9‐month observation, Gravitational‐wave high‐energy Electromagnetic Counterpart All‐sky Monitor (GECAM) has detected 147 bright Terrestrial Gamma‐ray Flashes (TGFs), 2 typical Terrestrial Electron Beams (TEBs), and 2 special TEB‐like eventsWith novel detector design, GECAM can effectively classify TGFs and TEBs, and reveal their fine temporal featuresWe obtained a very high TGF‐lightning association rate (∼80%) between GECAM and GLD360 in east Asia region During 9‐month observation, Gravitational‐wave high‐energy Electromagnetic Counterpart All‐sky Monitor (GECAM) has detected 147 bright Terrestrial Gamma‐ray Flashes (TGFs), 2 typical Terrestrial Electron Beams (TEBs), and 2 special TEB‐like events With novel detector design, GECAM can effectively classify TGFs and TEBs, and reveal their fine temporal features We obtained a very high TGF‐lightning association rate (∼80%) between GECAM and GLD360 in east Asia region

المؤلفون: HUANG, Q., WANG, G., HU, Y.-L., LIU, J.-X., YANG, J., WANG, S., ZHANG, H.-B.

المصدر: European Review for Medical & Pharmacological Sciences; 2017, Vol. 21 Issue 12, p2887-2894, 8p

مستخلص: OBJECTIVE: We investigated the significance of IL-1 and ICAM-1 in rat's subarachnoid hemorrhage (SAH) cerebral vasospasm (CVS) model. MATERIALS AND METHODS: A total of 30 Sprague-Dawley (SD) rats were randomly divided into the SAH group and the Sham group. Cisterna magna auto blood injection was used to prepare the CVS models. We studied and compared changes in the basilar arteries diameters before and after SAH. We measured the cerebrovascular inner diameter before and after SAH modeling using the ultrasound. ELISA method was used to measure the expression of IL-1 and ICAM-1 in peripheral blood. The expression of MAPK and P38 in the brain was tested using Western blot. Brain cells apoptosis was studied using TUNEL method. RESULTS: Cerebrovascular inner diameter reduced significantly in the SAH group as compared to the control group. The expression of IL-1 and ICAM-1 increased significantly after 48 hours. Compared to the Sham group, p-38 and p-MAPK expression levels in the SAH group increased significantly after 48 hours. Results showed that 48 hours after the operation, the level of apoptosis was significantly higher in the SAH group. IL-1 and ICAM-1 expression levels were associated with a P38-MAPK signal pathway in the brain. p38 and MAPK activation were closely related to apoptosis in the cortex. CONCLUSIONS: We suggest that the cerebral basilar vasospasm was occurred in rats 48 hours after ASH onset, with an increase in IL-1 and ICAM-1 expression and brain cells apoptosis. [ABSTRACT FROM AUTHOR]

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المؤلفون: JIN, H., ZHANG, H.-N., HOU, X.-L., ZHANG, B., WU, J., ZHANG, H.-B.

المصدر: European Review for Medical & Pharmacological Sciences; Jan2016, Vol. 20 Issue 1, p174-179, 6p

مستخلص: OBJECTIVE: To investigate the clinical effect of double dose of valsartan combined with tacrolimus in the treatment of diabetic nephropathy (DN). PATIENTS AND METHODS:HA total of 86 cases diagnosed with DN were selected from October 2013 to October 2014 in Zaozhuang Municipal Hospital, China. The study was approved by our hospital Ethics Committee and written consent was obtained from patients and their family members. Patients were randomly divided into three groups according to the sequence of admission, group A (conventional dose of valsartan group, n = 28 cases), group B (double dose of valsartan group, n = 29 cases) and group C (double dose of valsartan combined with tacrolimus group, n = 29). Clinical effects were compared by analyzing the renal function tests after 8 weeks. RESULTS: 24h urine protein, serum creatinine level of patients in group B and group C were significantly lower than that of group A. Those in group C was much lower. The glomerular filtration rates were significantly higher for group B and C than that of group A, and those in group C were much higher. The difference is statistically significant (p < 0.05). High-sensitivity C-reactive protein (hs CRP) and adiponectin levels of patients in group B and C of were significantly lower than that of group A and those in group C were much lower. The difference is statistically significant (p < 0.05). The high mobility group protein 1 (HMGB1) and renal tubular and interstitial damage index (TDI) of patients in B and C groups were significantly lower than those in the A group, and those in C group were significantly lower. The difference was statistically significant p < 0.05). The clinical effective rates of patients in group B and C were significantly higher than that in group A, and those of group C were much higher. The difference is statistically significant (p < 0.05). The recurrence rates of patients in group B and group C were significantly lower than those of group A and those in group C were much lower. The difference is statistically significant (p < 0.05). Patients in three groups showed no obvious drug complications. CONCLUSIONS: Double dose of valsartan combined with tacrolimus treatment of DN patients can improve clinical symptoms, reducing inflammation, inhibiting or even reversing the interstitial fibrosis, which will improve the curative effect and reduce the recurrence, as to provide a new theoretical basis for the clinical treatment of the disease. [ABSTRACT FROM AUTHOR]

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