المؤلفون: Cristina Terenzi, Gabriela Bermudez, Francesca Medri, Lara Davani, Vincenzo Tumiatti, Vincenza Andrisano, Serena Montanari, Angela De Simone

المصدر: Antioxidants, Vol 13, Iss 5, p 604 (2024)

مصطلحات موضوعية: agri-food by-products, polyphenols, antioxidant activity, circular economy, waste valorization, sustainable ingredients, Therapeutics. Pharmacology, RM1-950

الوصف: Agri-food by-products, obtained as waste from the food industry, negatively impact the global economy and the environment. In order to valorize waste materials from fruit juices and tomato sauces as upcycled materials rich in health-promoting compounds, they were characterized in terms of polyphenolic and protein content. The results obtained were compared with those collected for their final products. The recovery of polyphenols was performed via ultrasound-assisted extraction (UAE). A high-performance liquid chromatography–diode array detector (HPLC-DAD) method was developed and validated to depict the quali-quantitative polyphenolic profile of both the by-products and the final products. The antioxidant capacity of the resulting extracts was characterized by UV-Vis spectrophotometric assays in terms of total phenolic content (TPC) and total antioxidant status (TAS). Moreover, the protein content was assessed with the Kjeldahl method too. The results highlighted a significant quantity of polyphenols remaining in peach, apricot, and apple by-products, which were able to exert an antioxidant activity (in the range of 4.95 ± 5.69 × 10−1 to 7.06 ± 7.96 × 10−1 mmol Trolox 100 g−1 of dry weight (DW) sample). Conversely, the tomato by-products were highly rich in proteins (11.0 ± 2.00 to 14.4 ± 2.60 g of proteins 100 g−1 DW). The results proved that all by-products may potentially be sustainable ingredients with nutritional and functional value in a circular bio-economy prospect.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2076-3921/13/5/604; https://doaj.org/toc/2076-3921

URL الوصول: https://doaj.org/article/21f1f8323fd4441ba67f64b69e71b806

المؤلفون: Atalanti Christou, Nikolaos A. Parisis, Themistoklis Venianakis, Alexandra Barbouti, Andreas G. Tzakos, Ioannis P. Gerothanassis, Vlasios Goulas

المصدر: Antioxidants, Vol 12, Iss 10, p 1801 (2023)

مصطلحات موضوعية: Colocasia esculenta L., taro leaves, natural deep eutectic solvents (NaDESs), response surface methodology (RSM), ultrasound-assisted extraction (UAE), waste valorization, Therapeutics. Pharmacology, RM1-950

الوصف: Colocasia esculenta L. leaves are considered a by-product of taro cultivation and are discarded as environmental waste, despite their valuable phenolic composition. Their valorization to obtain value-added substances for medicinal, food, and cosmetic applications is the aim of the current work. An ultrasound-assisted extraction was developed for the environmentally friendly and sustainable isolation of taro leaf antioxidants using natural deep eutectic solvents (NaDESs). Among the utilized solvents, the NaDES based on betaine and ethylene glycol provided the best extraction efficiencies in terms of polyphenolic content and antioxidant activity. Multi-response optimization suggested a solvent-to-solid ratio of 10 mL g−1, a processing time of 60 min, an extraction temperature of 60 °C, and a water content of 33.8% (w/w) as optimal extraction parameters. Leaf extract obtained under these optimum operational parameters demonstrated a strong radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (65.80 ± 0.87%), a high ferric reducing antioxidant power (126.62 ± 1.92 μmol TE g−1 sample), and significant protection against oxidative stress-induced DNA damage. The chromatographic characterization of the optimum extract revealed its richness in flavonoids (flavones and flavonols). The outcomes of the present study suggest that the proposed method could serve as a highly efficient and green alternative for the recovery of polyphenols from agricultural wastes.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2076-3921/12/10/1801; https://doaj.org/toc/2076-3921

URL الوصول: https://doaj.org/article/c427f437454847738879354d8ed03aaa

المؤلفون: Esther Gómez-Mejía, Iván Sacristán, Noelia Rosales-Conrado, María Eugenia León-González, Yolanda Madrid

المصدر: Antioxidants, Vol 12, Iss 9, p 1742 (2023)

مصطلحات موضوعية: mandarin peels, polyphenols, enriched wheat bread, waste valorization, in vitro bioaccessibility, antioxidant activity, Therapeutics. Pharmacology, RM1-950

الوصف: The fortification of foods with bioactive polyphenols aims to improve their functional properties and to provide health benefits. Yet, to exert their benefits, phenolic compounds must be released from the food matrix and absorbed by the small intestine after digestion, so assessing their bioaccessibility is crucial to determine their potential role. This work aims to incorporate Citrus reticulata Blanco peel extracts into wheat bread as a promising opportunity to increase their bioactive potential, along with supporting the sustainable management of citrus-industry waste. A control and a wheat bread enriched at 2% and 4% (w/v) with a phenolic extract from mandarin peels were prepared and analyzed for antioxidant activity and phenolic composition using LC-MS and UV-Vis spectrophotometry. In addition, in vitro digestion was performed, and the digested extracts were analyzed with HPLC-MS/MS. The results showed a significant increase in total flavonoid content (TFC, 2.2 ± 0.1 mg·g−1), antioxidant activity (IC50 = 37 ± 4 mg·g−1), and contents of quercetin, caffeic acid, and hesperidin in the 4% (w/v) enriched bread. Yet, most polyphenols were completely degraded after the in vitro digestion process, barring hesperidin (159 ± 36 μg·g−1), highlighting the contribution of citrus enrichment in the development of an enriched bread with antioxidant potential.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2076-3921/12/9/1742; https://doaj.org/toc/2076-3921

URL الوصول: https://doaj.org/article/fd9d0f0f27274f2fb854c67f6f8bab54

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المؤلفون: Ana Marđokić, Angela Estefanía Maldonado, Katalin Klosz, Máté András Molnár, Gyula Vatai, Szilvia Bánvölgyi

المصدر: Antioxidants, Vol 12, Iss 6, p 1175 (2023)

مصطلحات موضوعية: microwave-assisted extraction, olive pomace, total polyphenols, antioxidant activity, waste valorization, Therapeutics. Pharmacology, RM1-950

الوصف: Olive pomace is a by-product of olive oil production that is toxic to the environment. The purpose of this study was to evaluate the methods of olive pomace valorization through the implementation of novel technology, the so-called microwave-assisted extraction process. To determine the total polyphenol content (TPC) and antioxidant activity (AA), polyphenol extraction using MAE was performed. Response surface methodology was used to determine the best extraction conditions, whereby the effects of three factors, solid ratio (g/50 mL), time (s), and power (W), were measured. The ferric reducing antioxidant power (FRAP) method was used to assess AA, whereas the spectrophotometric Folin–Ciocalteu (FC) method was used to determine TPC. The highest TPC of 15.30 mg of gallic acid equivalents per gram of dried weight (mg GAE/gdw) was generated after 105 s at 450 W, with a solid concentration of 1 g/50 mL, while the maximum AA was 10 mg of ascorbic acid equivalents per gram of dried weight (mg AAE/gdw). Numerical optimization revealed that 800 W, 180 s, and 1 g/50 mL were the best conditions for obtaining maximum TPC and AA.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2076-3921/12/6/1175; https://doaj.org/toc/2076-3921

URL الوصول: https://doaj.org/article/f4ec3f12c5b64ecea1e9a923481731fe

المؤلفون: Carmela Spagnuolo, Federica Moccia, Idolo Tedesco, Eva Adabbo, Lucia Panzella, Gian Luigi Russo, Alessandra Napolitano

المصدر: Antioxidants, Vol 12, Iss 2, p 421 (2023)

مصطلحات موضوعية: strawberry (Fragaria spp.), agri-food by-products, antioxidant, antiproliferative effect, fermentation, waste valorization, Therapeutics. Pharmacology, RM1-950

الوصف: The production of fruit distillates generates solid residues which are potentially rich in bioactive compounds worthy of valorization and exploitation. We report herein the in vitro antioxidant and antiproliferative properties of an extract obtained from the waste of fermented strawberry distillate production. The main low molecular weight phenolic components of the extract were identified as ellagic acid and p-coumaric acid using spectroscopic and chromatographic analysis. The extract exhibited high antioxidant properties, particularly in the ferric reducing/antioxidant power (FRAP) assay, and a high total phenolic content (TPC). It was also able to induce an antiproliferative effect on different human cancer cell lines. A strong decrease in viability in human promyelocytic leukemia (HL-60) cells through a rapid and massive apoptosis were observed. Moreover, at an early time (

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2076-3921/12/2/421; https://doaj.org/toc/2076-3921

URL الوصول: https://doaj.org/article/0c5b3585b2494b81830cd0b4ba558f2d

المؤلفون: Maria Luz Maia, Clara Grosso, M. Fátima Barroso, Aurora Silva, Cristina Delerue-Matos, Valentina Fernandes Domingues

المصدر: Antioxidants, Vol 12, Iss 2, p 435 (2023)

مصطلحات موضوعية: shrimp characterization, waste valorization, antioxidant activity, neuroprotective activity, antimicrobial activity, Therapeutics. Pharmacology, RM1-950

الوصف: The production and consumption of shrimp species create massive amounts of shrimp bio-waste. In this study, shrimp shell waste from Palaemon serratus and Palaemon varians from the Portuguese coast was characterized. Regarding the antioxidant capacity, the obtained values were between 4.7 and 10.4 mg gallic acid equivalents (GAE)/g dry weight (dw) for Total phenolic content (TPC); 3 and 7 mg ascorbic acid equivalents (AAE)/g dw for Ferric reducing antioxidant power assay (FRAP); 0.4 and 1.2 mg Trolox equivalent (TE)/g dw for 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical scavenging (DPPH•); 4 and 11 mg TE/g dw for 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity (ABTS•+); and 72 and 130 mg TE/g dw for Oxygen radical absorbance capacity (ORAC). For the antimicrobial activity, shrimp shell waste from P. varians formed inhibition zones between 14 and 23 mm. Total carotenoid content values were in the range of 28 and 134 μg/g dw, and according to their HPLC-PAD profile, β-carotene and astaxanthin contents were between 0.3 and 7.6 μg/g dw and 1.1 and 26.1 μg/g dw, respectively. These studies are critical to recognizing the potential added value of shrimp shell waste as possible colorants and preservatives with antioxidant protection capacity to be used in the food industry.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2076-3921/12/2/435; https://doaj.org/toc/2076-3921

URL الوصول: https://doaj.org/article/02d7abb661d2402f89396931c86e3cf3

المؤلفون: Valeria Pappalardo, Nicoletta Ravasio, Ermelinda Falletta, Maria Cristina De Rosa, Federica Zaccheria

المصدر: Antioxidants, Vol 12, Iss 2, p 218 (2023)

مصطلحات موضوعية: natural antioxidants, lipophilization, food waste valorization, sustainable chemistry, Therapeutics. Pharmacology, RM1-950

الوصف: A natural antioxidant, widely spread in plants, chlorogenic acid (CGA), can be lipophilized through a heterogeneous, non-enzymatic, catalytic process. Thus, sulfonic resins under no solvent conditions allow to obtain a series of esters in up to 93% yield through reaction of CGA with fatty alcohols of different chain length. The reaction takes place in one single step under mild conditions with conversions up to 96% and selectivity up to 99%. Product recovery in high purity was very easy and the esters obtained were fully characterized with spectroscopic techniques and through the DPPH test to verify the preservation of antioxidant activity. According to this test, all of them showed increased activity with respect to the parent acid and anyway higher than butylated hydroxyanisole. An in-silico method also suggested their very low toxicity. The increased lipophilicity of the esters allows their formulation in cosmetic and nutraceutic lipid-based products.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2076-3921/12/2/218; https://doaj.org/toc/2076-3921

URL الوصول: https://doaj.org/article/bcd3c8eded244150bca60f777f0d7153

المؤلفون: Luigi Castaldo, Luana Izzo, Sonia Lombardi, Anna Gaspari, Stefania De Pascale, Michela Grosso, Alberto Ritieni

المصدر: Antioxidants, Vol 11, Iss 12, p 2453 (2022)

مصطلحات موضوعية: bioactive compounds, orbitrap, polyphenols, agro-waste valorization, Therapeutics. Pharmacology, RM1-950

الوصف: The water-based extract of broad bean hulls contains several bioactive molecules, including polyphenols well-known to exert antioxidant activity, which could justify its use in nutraceutical formulations. Hence, the current investigation aimed to establish the polyphenolic profile of water-based extracts from broad bean hulls through UHPLC–Q-Orbitrap HRMS analysis. The findings highlighted that p-coumaric acid, chlorogenic acid, and epicatechin were the most common compounds found in the tested extracts, being quantified at a mean concentration of 42.1, 32.6, and 31.2 mg/100 g, respectively. Moreover, broad bean hull extracts were encapsulated into a nutraceutical formulation, after which the antioxidant properties and the bioaccessibility of phenolic compounds during the simulated gastrointestinal (GI) process were investigated and compared with the digested non-encapsulated extract. The data highlighted that following the GI process, the capsules were able to preserve active compounds from the adverse effects of digestion, resulting in a greater antioxidant capacity and polyphenol bioaccessibility in the duodenal and colonic phases, compared with the non-encapsulated extract. Our results showed that the water extract from broad bean hulls may be considered a valuable source of natural polyphenolic compounds; in addition, the use of a gastric-resistant capsule could be a suitable alternative to transport these bioactive compounds to the target tissues.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2076-3921/11/12/2453; https://doaj.org/toc/2076-3921

URL الوصول: https://doaj.org/article/5c214339f71548ee811e748b9f8214e8

المؤلفون: Guillermo Pascual, Diego Silva, Marisol Vargas, Mario Aranda, Juan Antonio Cañumir, María Dolores López

المصدر: Antioxidants, Vol 12, Iss 1, p 54 (2022)

مصطلحات موضوعية: winemaking industry, anthocyanins, Apis mellifera, antiviral activity, agro-sustainability, food waste valorization, Therapeutics. Pharmacology, RM1-950

الوصف: Ingredients rich in phenolic compounds and antioxidants of winemaking wastes, which play an important role in the prevention of various diseases and the control of viruses, are being explored. Currently, there is a concern about honeybee population loss, with deformed wing virus (DWV) being the most common virus infecting apiaries and one of the main causes of honeybee decline. Hence, the effect of grape pomace powder (GPP) as a dietary supplement to enhance the immune system of honeybees affected by DWV was evaluated. The characteristics of the ingredient GPP, obtained by spray-drying, revealed a high anthocyanin content (1102.45 mg 100 g−1), and it was applied at doses of 0.5, 1, 2.5 and 5% as a dietary supplement for bees infected by DWV. The results showed that the GPP treatments strengthened the immune response of honeybees against DWV. Moreover, the expression of the Relish gene was significantly higher in bees fed with GPP compared to the infected control. This study, which is framed in the search of food waste valorization for environmental sustainability, proves the feasibility of using grape wastes as dietary supplements for pollinators, and provides knowledge of the influence of polyphenols on the expression profiles of immune-related genes in honeybees.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2076-3921/12/1/54; https://doaj.org/toc/2076-3921

URL الوصول: https://doaj.org/article/24b5e54b7caf48099741aa0f1507d6dc