المؤلفون: Rosengren, L, Vasilcanu, D, Vasilcanu, R, Fickenscher, S, Sehat, B, Natalishvili, N, Naughton, S, Yin, SC, Girnita, A, Girnita, L, Axelson, M, Larsson, O

المصدر: Biochemical and biophysical research communications. 347(4):1059-1066

مصطلحات موضوعية: Medicin och hälsovetenskap

URL الوصول: http://kipublications.ki.se/Default.aspx?queryparsed=id:1932748

المؤلفون: Yin SC; Department of Otolaryngology - Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Department of Otolaryngology - Head and Neck Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China., Guo W, Tao ZZ

المصدر: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2013 Sep 13; Vol. 439 (1), pp. 1-5. Date of Electronic Publication: 2013 Aug 22.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE

مواضيع طبية MeSH: Antineoplastic Agents/*pharmacology , Nasopharyngeal Neoplasms/*drug therapy , Podophyllotoxin/*analogs & derivatives , Receptor, IGF Type 1/*antagonists & inhibitors, Animals ; Carcinoma ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival ; Dose-Response Relationship, Drug ; Drug Screening Assays, Antitumor ; Humans ; Inhibitory Concentration 50 ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Nasopharyngeal Carcinoma ; Neoplasm Metastasis ; Phosphorylation ; Podophyllotoxin/pharmacology ; Proto-Oncogene Proteins c-akt/metabolism ; Receptor, IGF Type 1/metabolism ; Somatomedins/metabolism ; Xenograft Model Antitumor Assays

مستخلص: Insulin-like growth factor-1 receptor (IGF-1R) is a cell membrane receptor with tyrosine kinase activity and plays important roles in cell transformation, tumor growth, tumor invasion, and metastasis. Picropodophyllin (PPP) is a selective IGF-1R inhibitor and shows promising antitumor effects for several human cancers. However, its antitumor effects in nasopharyngeal carcinoma (NPC) remain unclear. The purpose of this study is to investigate the antitumor activity of PPP in NPC using in vitro cell culture and in vivo animal model. We found that PPP dose-dependently decreased the IGF-induced phosphorylation and activity of IGF-1R and consequently reduced the phosphorylation of Akt, one downstream target of IGF-1R. In addition, PPP inhibited NPC cell proliferation in vitro. The half maximal inhibitory concentration (IC50) of PPP for NPC cell line CNE-2 was ≤1 μM at 24h after treatment and ≤0.5 μM at 48 h after treatment, respectively. Moreover, administration of PPP by intraperitoneal injection significantly suppressed the tumor growth of xenografted NPC in nude mice. Taken together, these results suggest targeting IGF-1R by PPP may represent a new strategy for treatment of NPCs with positive IGF-1R expression.
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