المؤلفون: Christoph Held, Tülay Köse, Sultan Mohammad, Irina Smirnova, Gabriele Sadowski, Thomas Gerlach, Emrah Altuntepe

المصدر: Fluid Phase Equilibria. 416:83-93

مصطلحات موضوعية: chemistry.chemical_classification, Aqueous solution, Chromatography, Chemistry, General Chemical Engineering, Aqueous two-phase system, Analytical chemistry, General Physics and Astronomy, Salt (chemistry), 02 engineering and technology, 010402 general chemistry, 01 natural sciences, 0104 chemical sciences, COSMO-RS, 020401 chemical engineering, Phase (matter), Binary system, 0204 chemical engineering, Physical and Theoretical Chemistry, Solubility, Ternary operation

الوصف: In biotechnological processes, salts might be present during reaction steps and in downstream processes. Salts are known to have a strong impact on phase equilibria of aqueous systems. In this work, the liquid–liquid equilibria (LLE) of ternary salt/MIBK/water mixtures were measured at 298.15 K and 1 bar up to the salt solubility limit. The salts studied in this work were NaCl, LiCl, KCl, NaNO3, LiNO3, Na2SO4, CH3COONa, and CH3COOLi. From these LLE measurements it was found that a high amount of salt is dissolved in the aqueous phase whereas only a very small amount of salt was detected in the MIBK phase. Further, the salting-out behavior of MIBK from the aqueous phase upon addition of different salts was investigated to study ion-specific effects. Two ion-specific models, ePC-SAFT and an extended version of COSMO-RS for electrolytes were used for modeling the binary system MIBK/water and ternary salt/MIBK/water systems. In case of the COSMO-RS based approach, the modeling results were fully predictive. In contrast, ion-specific binary interaction parameters between MIBK and ions were fitted to experimental LLE data of the ternary systems salt/MIBK/water when using ePC-SAFT. The results show that the COSMO-RS based approach allows for predicting the salt influence on LLE with acceptable accuracy, whereas ePC-SAFT allows for almost quantitative correlations of experimental data.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::ba69a830bf1487c90b7b15b18f2d2a56
https://doi.org/10.1016/j.fluid.2015.11.018

المؤلفون: Tim Zeiner, Sultan Mohammad, Ina Stark, Jan Muendges, Andrzej Górak

المصدر: Fluid Phase Equilibria. 385:227-236

مصطلحات موضوعية: Chromatography, Aqueous solution, Chemistry, Precipitation (chemistry), General Chemical Engineering, Sodium, Extraction (chemistry), Aqueous two-phase system, General Physics and Astronomy, chemistry.chemical_element, Polyethylene glycol, chemistry.chemical_compound, Yield (chemistry), Physical and Theoretical Chemistry, Solubility

الوصف: The current work investigates the extraction of a monoclonal antibody immunoglobulin G1 from a Chinese hamster ovary cell supernatant. Solubility of biologic substances was figured out as the most limiting factor during aqueous two-phase extraction. Therefore, it was majorly considered for system choice and for the determination of an operating window to prevent product loss due to precipitation. The solubility of immunoglobulin G1 was screened for different solutions of phase forming components. Best solubility was observed for a polyethylene glycol 2000 – phosphate aqueous two-phase system at pH six. The influence of additional sodium chloride and cell supernatant loading on the purification was investigated. Most promising extraction conditions were determined to either include no or a high amount of sodium chloride. A decrease of product phase (immunoglobulin G1-rich phase) volume further improves the purification resulting purification factors of up to 3.1 with an immunoglobulin G1 yield of higher than 90% within a single extraction step.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::350430983a0f2a094cd3d9d01035217a
https://doi.org/10.1016/j.fluid.2014.10.034