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المؤلفون: Joseph Passarelli, Mahmoud A. ElSohly, K. Bracher, Raymond A. Hui, Salvatore J. Salamone, Robert Sundoro Wu, C. Brenner, V.D. Ghodoussi, Rudolf Brenneisen
المصدر: Forensic science international. 79(1)
مصطلحات موضوعية: Adult, Male, Time Factors, Metabolite, Urine, Cross Reactions, Mass spectrometry, Sensitivity and Specificity, Gas Chromatography-Mass Spectrometry, Pathology and Forensic Medicine, Excretion, chemistry.chemical_compound, Methaqualone, Enzyme Multiplied Immunoassay Technique, medicine, Humans, Hypnotics and Sedatives, Immunoassay, Chromatography, medicine.diagnostic_test, Chemistry, Female, Gas chromatography, Gas chromatography–mass spectrometry, Law, medicine.drug
الوصف: A study was performed to compare the ONLINE and EMIT II immunoassays with gas chromatographic/mass spectrometric (GC/MS) analysis of methaqualone metabolites on urine using samples obtained from a clinical study. Urine was collected over a 72 h period from six healthy adults (4 male, 2 female) after oral dosing with 200 mg methaqualone (MTQ). Each urine sample was analyzed by ONLINE and EMIT II. The samples were then analyzed by GC/MS, hydrolyzed with beta-glucuronidase and again analyzed by GC/MS. Both immunoassays showed greater than 600 ng/ml concentrations of drug in each sample by the second void and remained highly positive for the rest of the 72 h. Unhydrolyzed samples analyzed by GC/MS showed both low concentrations of MTQ as well as its five major hydroxylated metabolites. The hydrolyzed samples analyzed by GC/MS showed high concentrations of the hydroxylated metabolites with the 2'-hydroxy and 3'-hydroxy metabolites being present at the highest concentrations, the 4'-hydroxy metabolite at a lower amount and the 6-hydroxy and 2-hydroxy metabolites at the lowest concentrations. The GC/MS data coupled with the antibody cross-reactivity data indicate that the major species in clinical samples that cross-react in both immunoassays are the conjugated forms of the hydroxylated metabolites of MTQ. Therefore when confirming by GC/MS after an immunoassay screen it would be prudent to confirm for the major hydroxylated metabolites as glucuronides of MTQ instead of the parent drug.
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