المؤلفون: Chakraborty, Avijit, Haque, Sk Moquammel, Dey, Diganta, Mukherjee, Swapna, Ghosh, Biswajit

المصدر: Proceedings of the National Academy of Sciences, India Section B; 20220101, Issue: Preprints p1-13, 13p

مستخلص: Every year, 150 million people become affected, worldwide, by severe forms of multi-drug-resistant urinary tract infections (UTI). Several antibiotics are being tested to combat the diseases but newer types of resistance are emerging every year. This throws the challenge of searching of an alternative source of antibacterial compounds. The present study was conducted to evaluate the antimicrobial activities of in vitro and ex vitro grown plants of Coleusforskohliiagainst twenty MDR strains of UTI bacteria. When methanolic leaf extracts were tested, seventeen among twenty UTI pathogens were found to be sensitive at only 0.75 mg/cup concentration, and three were resistant even at the 6.0 mg/cup concentration. Both the in vitro and ex vitro plants exhibited the highest efficacy against the pathogenic strain of Klebsiella(Isolate no-42285) with the ZI 17.66 ± 0.33 and 18.66 ± 0.57 mm, respectively. Naturally propagated in vivo plants showed significantly low activities compared to those of ex vitro grown plants. In vitro optimal multiplication of 20.4 ± 0.28 shoots per explant was achieved in MS medium supplemented with 1.0 mg/l 6-benzylaminopurine and 0.1 mg/l α-naphthalene acetic acid. A total of 38 and 35 compounds were detected from in vitro and ex vitro plant crude extract, respectively, through GC–MS including thiopene-2-carboxamide, 2-pyrazoline which are known to have potent antimicrobial activity. As both the ex vitro and in vitro plants were found to be highly efficient against most of the UTI pathogens tested, they may serve as a potential source of antimicrobial drug for an alternative therapy.