المؤلفون: Kamsteeg, E. J., Heijnen, I., van Os, C. H., Deen, P. M. T.

المصدر: The Journal of Cell Biology, 2000 Nov . 151(4), 919-929.

URL الوصول: https://www.jstor.org/stable/1620121

المؤلفون: van Os, C H, Mircheff, A K, Wright, E M

المصدر: The Journal of Cell Biology; April 1977, Vol. 73 Issue: 1 p257-260, 4p

مستخلص: This study reports on the distribution of bicarbonate-stimulated ATPase in rat intestinal epithelial cells. Brush-border membranes and basolateral membranes were separated from each other and from mitochondrial and other intracellular membranes by differential and density gradient centrifugation. Bicarbonate-sensitive ATPase activity followed the mitochondrial marker succinic dehydrogenase closely throughout all the centrifugation steps. The low HCO3--ATPase activity in purified brush-border and basolateral plasma membranes could be accounted for quantitatively by the small mitochondrial contamination. Consequently, there are no grounds for postulating that this enzyme has a direct role in H+ or HCO3- transport across the rat small intestine.