المؤلفون: Watanabe D; National Research Institute of Brewing, Higashihiroshima, Hiroshima, Japan., Araki Y, Zhou Y, Maeya N, Akao T, Shimoi H

المصدر: Applied and environmental microbiology [Appl Environ Microbiol] 2012 Jun; Vol. 78 (11), pp. 4008-16. Date of Electronic Publication: 2012 Mar 23.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE

مواضيع طبية MeSH: Frameshift Mutation*, Alcoholic Beverages/*microbiology , Ethanol/*metabolism , Protein Kinases/*genetics , Saccharomyces cerevisiae/*enzymology , Saccharomyces cerevisiae/*physiology , Saccharomyces cerevisiae Proteins/*genetics, Fermentation ; Japan ; Protein Kinases/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/metabolism

مستخلص: Sake yeast cells have defective entry into the quiescent state, allowing them to sustain high fermentation rates. To reveal the underlying mechanism, we investigated the PAS kinase Rim15p, which orchestrates initiation of the quiescence program in Saccharomyces cerevisiae. We found that Rim15p is truncated at the carboxyl terminus in modern sake yeast strains as a result of a frameshift mutation. Introduction of this mutation or deletion of the full-length RIM15 gene in a laboratory strain led to a defective stress response, decreased synthesis of the storage carbohydrates trehalose and glycogen, and impaired G(1) arrest, which together closely resemble the characteristic phenotypes of sake yeast. Notably, expression of a functional RIM15 gene in a modern sake strain suppressed all of these phenotypes, demonstrating that dysfunction of Rim15p prevents sake yeast cells from entering quiescence. Moreover, loss of Rim15p or its downstream targets Igo1p and Igo2p remarkably improved the fermentation rate in a laboratory strain. This finding verified that Rim15p-mediated entry into quiescence plays pivotal roles in the inhibition of ethanol fermentation. Taken together, our results suggest that the loss-of-function mutation in the RIM15 gene may be the key genetic determinant of the increased ethanol production rates in modern sake yeast strains.