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    الوصف: Additional file 4: Supplementary Figure 4. WNT16 treatment inhibited ALP activity in AS-osteoprogenitors. AS-osteoprogenitors were plated in 96 wells plate and treated with 0, 25, or 50 ng/ml WNT16, and then analyzed as indicated by (A) WST assay for cell proliferation. (B) ALP and COL staining, and (C) ALP activity. Data are presented as the mean �� SD (n=3). *p

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    مصطلحات موضوعية: animal structures

    الوصف: Additional file 2: Supplementary Figure 2. The result of siRNA efficiency test. (A) Total four human osteoblasts cell lines (MG63, FOB, SaOS2, U2OS) were extracted total RNA and analyzed by RT-PCR to test WNT16 level. (B) FOB cell were transfected with five siRNA oligo against human WNT16 and analyzed by RT-PCR for siRNA efficiency.

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