المؤلفون: Ninkuu V; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Yan J; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Zhang L; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Fu Z; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Yang T; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Li S; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Li B; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Duan J; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Ren J; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Li G; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Yang X; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China., Zeng H; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, (CAAS), Beijing, China.

المصدر: Frontiers in plant science [Front Plant Sci] 2022 Sep 23; Vol. 13, pp. 980821. Date of Electronic Publication: 2022 Sep 23 (Print Publication: 2022).

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101568200 Publication Model: eCollection Cited Medium: Print ISSN: 1664-462X (Print) Linking ISSN: 1664462X NLM ISO Abbreviation: Front Plant Sci Subsets: PubMed not MEDLINE

مستخلص: Magnaporthe oryzae is a potent fungus that adversely affects rice yield. Combinatorial techniques of prevention, toxic chemicals, and fungicide are used to remedy rice blast infection. We reported the role of Hrip1 in cell death elicitation and expression of systematic acquired resistance that could potentially stifle M. oryzae infection. In this study, transcriptome and metabolomic techniques were used to investigate the mechanism by which Hrip1 reprogramed the transcriptome of rice seedlings to confer immunity against M. oryzae . Our results showed that Hrip1 induces cell wall thickening and phytoalexin elicitation to confer immunity against M. oryzae infection. Hrip1 activates key lignin biosynthetic genes and myeloblastosis transcription factors that act as molecular switches for lignin production. Lignin content was increased by 68.46% and more after 48 h onwards in Hrip1-treated seedlings compared to the control treatment. Further analysis of cell wall morphology using the transmission electron microscopy technique revealed over 100% cell wall robustness. Hrip1 also induced the expression of 24 diterpene synthases. These include class I and II terpene synthases, cytochrome P450 subfamilies (OsCYP76M and OsCYP71Z), and momilactones synthases. The relationship between the expression of these genes and metabolic elicitation was analyzed using ultra-performance liquid chromatography-tandem mass spectrometry. Enhanced amounts of momilactones A and B, oryzalactone, and phytocassane A and G were detected in the Hrip1-treated leaves. We also identified seven benzoxazinoid genes (BX1-BX7) that could improve rice immunity. Our findings show that Hrip1 confers dual immunity by leveraging lignin and phytoalexins for physical and chemical resistance. This study provides novel insights into the mechanisms underlying Hrip1-treated plant immunity.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.The reviewer J-YC declared a shared affiliation with the authors to the handling editor at the time of review.
(Copyright © 2022 Ninkuu, Yan, Zhang, Fu, Yang, Li, Li, Duan, Ren, Li, Yang and Zeng.)