المؤلفون: Shen J; School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. jinboshen@cuhk.edu.hk.

المصدر: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1662, pp. 223-230.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

مواضيع طبية MeSH: Secretory Pathway*, Arabidopsis/*metabolism , Green Fluorescent Proteins/*metabolism , Microscopy, Fluorescence/*methods , Protoplasts/*metabolism , Vacuoles/*metabolism, Arabidopsis/genetics ; Arabidopsis/ultrastructure ; Endoplasmic Reticulum/chemistry ; Endoplasmic Reticulum/metabolism ; Endoplasmic Reticulum/ultrastructure ; Endosomes/chemistry ; Endosomes/metabolism ; Endosomes/ultrastructure ; Gene Expression ; Genes, Reporter ; Golgi Apparatus/chemistry ; Golgi Apparatus/metabolism ; Golgi Apparatus/ultrastructure ; Green Fluorescent Proteins/genetics ; Hydrogen-Ion Concentration ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Oligopeptides/genetics ; Oligopeptides/metabolism ; Plant Leaves/genetics ; Plant Leaves/metabolism ; Plant Leaves/ultrastructure ; Protein Sorting Signals/genetics ; Protoplasts/chemistry ; Protoplasts/ultrastructure ; Transformation, Genetic ; Vacuoles/chemistry ; Vacuoles/ultrastructure ; Red Fluorescent Protein

المؤلفون: Shen W; Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China., Xiao Z; Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China., Shen J; State Key Laboratory of Agrobiotechnology, Centre for Cell and Developmental Biology, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China., Gao C; Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China. gaocaiji@163.com.

المصدر: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1662, pp. 75-86.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

مواضيع طبية MeSH: Arabidopsis/*metabolism , Golgi Apparatus/*metabolism , Microscopy, Fluorescence/*methods , Plant Cells/*metabolism , Protoplasts/*metabolism , Secretory Pathway/*genetics, Agrobacterium tumefaciens/genetics ; Agrobacterium tumefaciens/metabolism ; Arabidopsis/drug effects ; Arabidopsis/genetics ; Brefeldin A/pharmacology ; COP-Coated Vesicles/drug effects ; COP-Coated Vesicles/metabolism ; COP-Coated Vesicles/ultrastructure ; Cells, Cultured ; Dexamethasone/pharmacology ; Electroporation/methods ; Endoplasmic Reticulum/drug effects ; Endoplasmic Reticulum/metabolism ; Endoplasmic Reticulum/ultrastructure ; Glycosylation/drug effects ; Golgi Apparatus/drug effects ; Golgi Apparatus/ultrastructure ; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase/chemistry ; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase/chemistry ; Plant Cells/drug effects ; Plant Cells/ultrastructure ; Plants, Genetically Modified ; Plasmids/chemistry ; Plasmids/metabolism ; Protein Transport/drug effects ; Protoplasts/drug effects ; Protoplasts/ultrastructure ; Secretory Pathway/drug effects ; Transfection/methods

المؤلفون: Guo J; Department of Bioengineering, Sichuan Agricultural University, Sichuan, 625014, China., Miao Y; School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore.; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, 637459, Singapore., Cai Y; Department of Bioengineering, Sichuan Agricultural University, Sichuan, 625014, China. yicai@sicau.edu.cn.

المصدر: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1662, pp. 87-95.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

مواضيع طبية MeSH: Cell Membrane/*metabolism , Endoplasmic Reticulum/*metabolism , Golgi Apparatus/*metabolism , Microscopy, Fluorescence/*methods , Protoplasts/*metabolism , Secretory Pathway/*genetics , Nicotiana/*metabolism, ADP-Ribosylation Factor 1/genetics ; ADP-Ribosylation Factor 1/metabolism ; Blotting, Western/methods ; Cell Membrane/ultrastructure ; Cells, Cultured ; Electroporation/methods ; Endoplasmic Reticulum/ultrastructure ; Genes, Reporter ; Golgi Apparatus/ultrastructure ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Microsomes/metabolism ; Microsomes/ultrastructure ; Plant Cells/metabolism ; Plant Cells/ultrastructure ; Plants, Genetically Modified ; Plasmids/chemistry ; Plasmids/metabolism ; Protein Transport ; Protoplasts/ultrastructure ; Nicotiana/genetics ; Transfection/methods

المؤلفون: Fäßler F; Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany., Pimpl P; Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany. peter.pimpl@zmbp.uni-tuebingen.de.; Department of Biology, Southern University of Science and Technology (SUSTech), Shenzhen, Guangdong, 518055, China. peter.pimpl@zmbp.uni-tuebingen.de.

المصدر: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1662, pp. 159-170.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

مواضيع طبية MeSH: Epitopes/*metabolism , Fluorescence Resonance Energy Transfer/*methods , Microscopy, Fluorescence/*methods , Plant Proteins/*metabolism , Single-Domain Antibodies/*metabolism , Nicotiana/*metabolism, Epitopes/chemistry ; Gene Expression ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Optical Imaging/methods ; Plant Proteins/genetics ; Protein Binding ; Protoplasts/metabolism ; Protoplasts/ultrastructure ; Single-Domain Antibodies/chemistry ; Nicotiana/ultrastructure ; Red Fluorescent Protein

المؤلفون: Laptenok SP; Laboratoire d'Optique et Biosciences, INSERM U696-CNRS UMR7645, Ecole Polytechnique, Palaiseau, France., Snellenburg JJ, Bücherl CA, Konrad KR, Borst JW

المصدر: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2014; Vol. 1076, pp. 481-502.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

مواضيع طبية MeSH: Fluorescence Resonance Energy Transfer/*methods , Microscopy, Fluorescence/*methods , Plant Cells/*ultrastructure, Green Fluorescent Proteins/chemistry ; Optical Imaging ; Protoplasts/ultrastructure ; Signal-To-Noise Ratio ; Software

المؤلفون: Pyke K; Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire, UK. Kevin.Pyke@nottingham.ac.uk

المصدر: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2011; Vol. 774, pp. 19-32.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

مواضيع طبية MeSH: Organelle Size*, Arabidopsis/*cytology , Arabidopsis/*metabolism , Microscopy, Fluorescence/*methods , Plastids/*metabolism, Fluorescent Dyes/metabolism ; Imaging, Three-Dimensional ; Light ; Mesophyll Cells/cytology ; Mesophyll Cells/metabolism ; Microscopy, Confocal ; Plant Roots/cytology ; Plant Roots/metabolism ; Protoplasts/cytology ; Protoplasts/metabolism

المؤلفون: Hasek J; Laboratory of Cell Reproduction, Institute of Microbiology Academy of Sciences of the Czech Republic, Praha, Czech Republic., Streiblová E

المصدر: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 1996; Vol. 53, pp. 391-405.

نوع المنشور: Journal Article; Review

بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Print ISSN: 1064-3745 (Print) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

مواضيع طبية MeSH: Microscopy, Fluorescence/*methods , Mycology/*methods , Yeasts/*ultrastructure, Actins/analysis ; Animals ; Antibodies, Monoclonal/immunology ; Cell Nucleus/ultrastructure ; Fluorescent Antibody Technique, Indirect ; Fluorescent Dyes ; Fungal Proteins/analysis ; Mice ; Mitochondria/ultrastructure ; Protoplasts/ultrastructure ; Swine ; Yeasts/chemistry ; Yeasts/immunology