المؤلفون: Sharif F; Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore, Pakistan., Roman S; Department of Materials Science & Engineering, Kroto Research Institute, University of Sheffield, Broad Lane, Sheffield, UK., Asif A; Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore, Pakistan., Gigliobianco G; Department of Materials Science & Engineering, Kroto Research Institute, University of Sheffield, Broad Lane, Sheffield, UK., Ghafoor S; Department of Oral Biology, University of Health Sciences Lahore, Lahore, Pakistan., Tariq M; Department of Biology, Syed Babar Ali School of Science and Engineering, Lahore University of Management Sciences, Lahore, Pakistan., Siddiqui SA; Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore, Pakistan., Mahmood F; Department of Pediatric Plastic Surgery, Children Hospital, Lahore, Pakistan., Muhammad N; Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore, Pakistan., Rehman IU; Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore, Pakistan.; Department of Materials Science & Engineering, Kroto Research Institute, University of Sheffield, Broad Lane, Sheffield, UK., MacNeil S; Department of Materials Science & Engineering, Kroto Research Institute, University of Sheffield, Broad Lane, Sheffield, UK.

المصدر: Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2019 Jul; Vol. 13 (7), pp. 1178-1189. Date of Electronic Publication: 2019 May 14.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: John Wiley & Sons Country of Publication: England NLM ID: 101308490 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-7005 (Electronic) Linking ISSN: 19326254 NLM ISO Abbreviation: J Tissue Eng Regen Med Subsets: MEDLINE

مواضيع طبية MeSH: Cleft Palate*/metabolism , Cleft Palate*/pathology , Cleft Palate*/therapy , Materials Testing* , Membranes, Artificial*, Coated Materials, Biocompatible/*pharmacology, Animals ; Cell Line ; Coated Materials, Biocompatible/chemistry ; Heparin/chemistry ; Heparin/pharmacology ; Humans ; Male ; Mice ; Osteoblasts/metabolism ; Osteoblasts/pathology ; Polyesters/chemistry ; Polyesters/pharmacology ; Polyurethanes/chemistry ; Polyurethanes/pharmacology ; Rats ; Rats, Wistar

مستخلص: An oronasal fistula is a passage between the oral and nasal cavity. Currently, surgical procedures use mucosal flaps or collagen grafts to make a barrier between oral and nasal cavities. Our aim was to develop a cell-free synthetic repair material for closure of nasal fistulas. We surface functionalized electrospun polyurethane (PU) and poly-L-lactic acid (PLLA) and composite polymer (PU-PLLA) membranes with acrylic acid through plasma polymerization. Membranes were treated in a layer-by-layer approach to develop highly charged electrostatic layer that could bind heparin as a pro-angiogenic glycosaminoglycan. The properties were evaluated through physical, chemical, and mechanical characterization techniques. Cytotoxicity was tested with MC3T3 pre-osteoblast cell lines for 3, 7, and 14 days, and vasculogenesis was assessed by implantation into the chorio-allantoic membrane in chick embryos for 7 days. In vivo biocompatibility was assessed by subcutaneous implantation in rats for 1, 3, and 6 weeks. The membranes consisted of random fibers of PLLA-PU with fiber diameters of 0.47 and 0.12 μm, respectively. Significantly higher cell proliferation and migration of MC3T3 cells at 3, 7, and 14 days were shown on plasma-coated membranes compared with uncoated membranes. Further, it was found that plasma-coated membranes were more angiogenic than controls. In vivo implantation of membranes in rats did not reveal any gross toxicity to the materials, and wound healing was comparable with the native tissue repair (sham group). We therefore present a plasma-functionalized electrospun composite polymer membrane for use in the treatment of fistulas. These membranes are flexible, non-cytotoxic, and angiogenic, and we hope it should lead to permanent closure of oronasal fistula.
(© 2019 John Wiley & Sons, Ltd.)

المؤلفون: Irfan M; Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad (CUI), Lahore Campus, Defence Road, Off Raiwind Road, Lahore, Pakistan.; Faculty of Chemical and Energy Engineering, Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia.; Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia., Irfan M; Department of Chemistry, COMSATS University Islamabad (CUI), Lahore Campus, Defence Road, Off Raiwind Road, Lahore, Pakistan., Idris A; Faculty of Chemical and Energy Engineering, Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia.; Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia., Baig N; Chemistry Department, King Fahd University of Petroleum and Minerals, Dhahran, 31261, Saudi Arabia., Saleh TA; Chemistry Department, King Fahd University of Petroleum and Minerals, Dhahran, 31261, Saudi Arabia., Nasiri R; Faculty of Chemical and Energy Engineering, Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia.; Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia., Iqbal Y; Faculty of Science, Technology and Human Development, University Tun Hussein Onn Malaysia, 86400 Parit Raja, Johor, Malaysia., Muhammad N; Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad (CUI), Lahore Campus, Defence Road, Off Raiwind Road, Lahore, Pakistan., Rehman F; Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad (CUI), Lahore Campus, Defence Road, Off Raiwind Road, Lahore, Pakistan., Khalid H; Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad (CUI), Lahore Campus, Defence Road, Off Raiwind Road, Lahore, Pakistan.

المصدر: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2019 Mar; Vol. 107 (3), pp. 513-525. Date of Electronic Publication: 2018 Nov 28.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 101234237 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4965 (Electronic) Linking ISSN: 15493296 NLM ISO Abbreviation: J Biomed Mater Res A Subsets: MEDLINE

مواضيع طبية MeSH: Materials Testing* , Membranes, Artificial*, Nanocomposites/*chemistry , Nanotubes, Carbon/*chemistry , Povidone/*chemistry , Renal Dialysis/*instrumentation, Humans

مستخلص: This study focused to optimize the performance of polyethersulfone (PES) hemodialysis (HD) membrane using carboxylic functionalized multiwall carbon nanotubes (c-MWCNT) and lower molecular weight grade of polyvinylpyrrolidone (PVP-k30). Initially, MWCNT were chemically functionalized by acid treatment and nanocomposites (NCs) of PVP-k30 and c-MWCNT were formed and subsequently blended with PES polymer. The spectra of FTIR of the HD membranes revealed that NCs has strong hydrogen bonding and their addition to PES polymer improved the capillary system of membranes as confirmed by Field Emission Scanning Electron Microscope (FESEM) and leaching of the additive decreased to 2% and hydrophilicity improved to 22%. The pore size and porosity of NCs were also enhanced and rejection rate was achieved in the establish dialysis range (<60 kDa). The antifouling studies had shown that NCs membrane exhibited 30% less adhesion of protein with 80% flux recovery ratio. The blood compatibility assessment disclosed that NCs based membranes showed prolonged thrombin and prothrombin clotting times, lessened production of fibrinogen cluster, and greatly suppressed adhesion of blood plasma than a pristine PES membrane. The results also unveiled that PVP-k30/NCs improved the surface properties of the membrane and the urea and creatinine removal increased to 72% and 75% than pure PES membranes. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 513-525, 2019.
(© 2018 Wiley Periodicals, Inc.)