المؤلفون: Ferré PJ; UMR181 de Physiopathologie et Toxicologie Expérimentales, INRA, ENVT, National Veterinary School, 23 chemin des Capelles, BP 87614, Toulouse cedex 03, 31076, France., Liaubet L, Concordet D, SanCristobal M, Uro-Coste E, Tosser-Klopp G, Bonnet A, Toutain PL, Hatey F, Lefebvre HP

المصدر: Pharmaceutical research [Pharm Res] 2007 Aug; Vol. 24 (8), pp. 1480-9. Date of Electronic Publication: 2007 Mar 23.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 8406521 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0724-8741 (Print) Linking ISSN: 07248741 NLM ISO Abbreviation: Pharm Res Subsets: MEDLINE

مواضيع طبية MeSH: Gene Expression Profiling*, Muscle Proteins/*genetics , Muscle, Skeletal/*metabolism, Animals ; Collagen/genetics ; Collagen Type I/genetics ; Collagen Type I, alpha 1 Chain ; Down-Regulation/genetics ; Fibronectins/genetics ; Injections, Intramuscular/adverse effects ; Male ; Matrix Metalloproteinase 2/genetics ; Muscle, Skeletal/injuries ; Oligonucleotide Array Sequence Analysis/methods ; Osteonectin/genetics ; Swine ; Thymosin/genetics ; Time Factors ; Up-Regulation/genetics ; ras Proteins/genetics

مستخلص: Purpose: The purpose of this study is to describe the time course of gene expression in a skeletal muscle local injury induced by an intramuscular (IM) injection, and to compare the dynamics of gene expression with pathological events.
Materials and Methods: Ten piglets received 4 IM injections of propylene glycol in the longissimus dorsi muscles 6 h, 2, 7, and 21 days before euthanasia, where control and injected muscle sites were sampled for RNA isolation and microscopic examination. The hybridization of nylon cDNA microarrays was carried out with radioactive probes obtained from the muscle RNA.
Results: 153 genes were found under- or over-expressed at least once among the investigated time-conditions. The eight most discriminant genes were also identified: Two genes (GTP-binding protein RAD and Ankyrin repeat domain protein) were over-expressed at 6 h and six genes between 2 and 21 days (Osteonectin, Fibronectin, Matrix metalloproteinase-2, Collagen alpha 1(I) chain, Collagen alpha 2(I) chain, and Thymosin beta-4). Necrosis, inflammation and regeneration were observed through both the dynamics of gene expression profiles and through the microscopic examinations.
Conclusion: Our data demonstrate that several pathways are involved in post-injection muscle injury, and that necrosis, inflammation and regeneration are not sequential but occur in parallel.