المؤلفون: Rod Monroy, Bo Overschmidt, Paul W. Holzer, Laurence Zhu, Amon-Ra Gama, Curtis L. Cetrulo, Alexandre G. Lellouch, Angelo A. Leto Barone, Zhi Yang Ng, Krysta Moulton, Ivy A. Rosales

المصدر: J Burn Care Res

مصطلحات موضوعية: Pathology, medicine.medical_specialty, Swine, Cryopreservation, 03 medical and health sciences, 0302 clinical medicine, In vivo, Suidae, Medicine, Animals, Severe burn, Porcine skin, biology, business.industry, Genetically engineered, Rehabilitation, 030208 emergency & critical care medicine, Original Articles, Skin Transplantation, biology.organism_classification, In vitro, Disease Models, Animal, Emergency Medicine, Surgery, business, Cadaveric spasm, Burns, 030217 neurology & neurosurgery

الوصف: Vital, genetically engineered, porcine xenografts represent a promising alternative to human cadaveric allografts (HCA) in the treatment of severe burns. However, their clinical value would be significantly enhanced if preservation and long-term storage—without the loss of cellular viability—were feasible. The objective of this study was to examine the direct impact of cryopreservation and the length of storage on critical in vivo and in vitro parameters, necessary for a successful, potentially equivalent substitute to HCA. In this study, vital, porcine skin grafts, continuously cryopreserved for more than 7 years were compared side-by-side to otherwise identically prepared skin grafts stored for only 15 minutes. Two major histocompatibility complex (MHC)-controlled donor–recipient pairs received surgically created deep-partial wounds and subsequent grafting with split-thickness porcine skin grafts, differentiated only by the duration of storage. Clinical and histological outcomes, as well as quantification of cellular viability via a series of 3-4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays, were assessed. No statistically significant differences were observed between skin grafts cryopreserved for 15 minutes vs 7 years. Parametric distinctions between xenografts stored for short- vs long-term durations could not be ascertained across independent clinical, histological, or in vitro evaluative methods. The results of this study validate the ability to reliably preserve, store, and retain the essential metabolic activity of porcine tissues after cryopreservation. Plentiful, safe, and readily accessible inventories of vital xenografts represent an advantageous solution to numerous limitations associated with HCA, in the treatment of severe burns.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08e7d2a47d8e9aeba9ed2ab05c9f9386
https://europepmc.org/articles/PMC7030074/