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المؤلفون: Sung CR; Department of Bio Health Science, College of Natural Science, Changwon National University, Changwon, Republic of Korea., Kim BJ; Nonclinical Research Center, Chemon Inc., Yongin, Republic of Korea., Park CJ; Department of Bio Health Science, College of Natural Science, Changwon National University, Changwon, Republic of Korea., Oh IA; Department of Bio Health Science, College of Natural Science, Changwon National University, Changwon, Republic of Korea., Lee YJ; Department of Bio Health Science, College of Natural Science, Changwon National University, Changwon, Republic of Korea., Park YR; Department of Bio Health Science, College of Natural Science, Changwon National University, Changwon, Republic of Korea., Kwack SJ; Department of Bio Health Science, College of Natural Science, Changwon National University, Changwon, Republic of Korea.

المصدر: Journal of toxicology and environmental health. Part A [J Toxicol Environ Health A] 2024 Mar 18; Vol. 87 (6), pp. 266-273. Date of Electronic Publication: 2024 Jan 10.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 100960995 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1528-7394 (Print) Linking ISSN: 00984108 NLM ISO Abbreviation: J Toxicol Environ Health A Subsets: MEDLINE

مواضيع طبية MeSH: Orchiectomy* , Antineoplastic Agents*/pharmacology, Mice ; Rats ; Male ; Animals ; Rats, Sprague-Dawley ; Androgen Antagonists/pharmacology ; Benzophenones/toxicity ; Organ Size ; Genitalia, Male

مستخلص: Benzophenone-3 (BP-3, 2-hydroxy-4-methoxybenzophenone, oxybenzone) is one of the most widely used types of benzophenone organic sunscreen. However, this compound is a potentially harmful toxicant. The aim of this study was 2-fold to: (1) utilize a Hershberger bioassay in vivo in castrated male Sprague-Dawley rats to investigate the anti-androgenic activities of BP-3, and (2) use in vitro a methyl tetrazolium assay to compare the toxicity between Leydig cells (TM3 cells) and mouse fibroblast (NIH-3T3) cell lines. In the Hershberger assay, rats were divided into 6 groups (each of n  = 7): a vehicle control, negative control, positive control, PB-3 low (40 mg/kg), BP-3 intermediate (200 mg/kg), and BP-3 high (1000 mg/kg)-dose. The weight of the ventral prostate was significantly decreased at BP-3 doses of 200 or 1,000 mg/kg/day. In addition, the levator anibulbocavernosus muscle weights were also significantly reduced at BP-3 doses of 40, 200, or 1,000 mg/kg/day. In the MTT assay, the viability of NIH-3T3 mouse fibroblast cells was within the normal range. However, the TM3 mouse testis Leydig cell viability was significantly lowered in a concentration-dependent manner. Therefore, data indicate that BP-3 might exert in vivo anti-androgenic and in vitro cytotoxic effects in cells associated with the male reproductive system compared to normal non-reproductive cells. Abbreviation: BP-3: benzophenone-3; CG: Cowper's gland; DMEM: Dulbecco's modified Eagle's medium; DMSO: dimethyl sulfoxide; GP: glans penis; LABC: levator anibulbocavernosus muscle; MTT: methyl tetrazolium; NC: negative control; PC: positive control; SV: seminal vesicle; TP: testosterone propionate; VC: vehicle control; VP: ventral prostate.

المؤلفون: Patil MP; Industry-University Cooperation Foundation, Pukyong National University, Busan, Republic of Korea., Kim JO; Department of Microbiology, Pukyong National University, Busan, Republic of Korea.; School of Marine and Fisheries Life Science, Pukyong National University, Busan, Republic of Korea., Lee YJ; Department of Marine Biology, Pukyong National University, Busan, Republic of Korea., Seo YB; Research Institute for Basic Science, Pukyong National University, Busan, Republic of Korea., Kim JK; Department of Marine Biology, Pukyong National University, Busan, Republic of Korea., Kim GD; Department of Microbiology, Pukyong National University, Busan, Republic of Korea.; School of Marine and Fisheries Life Science, Pukyong National University, Busan, Republic of Korea.

المصدر: Mitochondrial DNA. Part B, Resources [Mitochondrial DNA B Resour] 2023 Mar 13; Vol. 8 (3), pp. 389-392. Date of Electronic Publication: 2023 Mar 13 (Print Publication: 2023).

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 101676334 Publication Model: eCollection Cited Medium: Internet ISSN: 2380-2359 (Electronic) Linking ISSN: 23802359 NLM ISO Abbreviation: Mitochondrial DNA B Resour Subsets: PubMed not MEDLINE

مستخلص: Japanese butterflyfish ( Chaetodon nippon ) belong to the family Chaetodontidae and order Chaetodontiformes. It has circular mitochondrial genome of 16,507 bp in length with 55.4% of A + T content and has 37 genes, including 22 tRNA, 2 rRNA, and 13 protein-coding genes, in addition to a control region. The results of phylogenetic analysis indicated that the C. nippon, C. wiebeli , C. auripes , C. auriga , C. octofasciatus , C. speculum , and C. modestus are closely related to each other. The findings of this study will provide useful genetic information for further phylogenetic and taxonomic classifications of Chaetodontidae.
Competing Interests: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.
(© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)

المؤلفون: Jeong SY; College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea., Choi WS; College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea., Kwon OS; College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea., Lee JS; National Institute of Biological Resources, Incheon, Republic of Korea., Son SY; Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea., Lee CH; Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.; Research Institute for Bioactive-Metabolome Network, Konkuk University, Seoul, Republic of Korea., Lee S; National Institute of Biological Resources, Incheon, Republic of Korea., Song JY; College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea., Lee YJ; College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea., Lee JY; College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.

المصدر: Pharmaceutical biology [Pharm Biol] 2022 Dec; Vol. 60 (1), pp. 1148-1159.

نوع المنشور: Journal Article; Randomized Controlled Trial, Veterinary

بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 9812552 Publication Model: Print Cited Medium: Internet ISSN: 1744-5116 (Electronic) Linking ISSN: 13880209 NLM ISO Abbreviation: Pharm Biol Subsets: MEDLINE

مواضيع طبية MeSH: Inflammation Mediators*/metabolism , Pinus*, Animals ; Anti-Inflammatory Agents/therapeutic use ; Antioxidants/pharmacology ; Dinoprostone/metabolism ; Inflammation/pathology ; Interleukin-1beta/metabolism ; Lipopolysaccharides/pharmacology ; Macrophages ; Mice ; Mice, Inbred ICR ; Plant Extracts/therapeutic use ; RAW 264.7 Cells ; Reactive Oxygen Species/metabolism ; Superoxide Dismutase/metabolism

مستخلص: Context: Pinus densiflora Siebold & Zucc. (Pinaceae) needle extracts ameliorate oxidative stress, but research into their anti-inflammatory effects is limited.
Objective: To investigate antioxidant and anti-inflammatory effects of a Pinus densiflora needles (PINE) ethanol extract in vitro and in vivo .
Materials and Methods: We measured levels of reactive oxygen species (ROS), superoxide dismutase (SOD) and inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells at various PINE concentrations (25, 50 and 100 μg/mL; but 6.25, 12.5 and 25 μg/mL for interleukin-1β and prostaglandin E 2 (PGE 2 )). Thirty ICR mice were randomized to six groups: vehicle, control, PINE pre-treatment (0.1, 0.3 and 1 mg/left ear for 10 min followed by arachidonic acid treatment for 30 min) and dexamethasone. The posttreatment ear thickness and myeloperoxidase (MPO) activity were measured.
Results: PINE 100 μg/mL significantly decreased ROS (IC 50 , 70.93 μg/mL, p  < 0.01), SOD (IC 50 , 30.99 μg/mL, p  < 0.05), malondialdehyde ( p  < 0.01), nitric oxide (NO) (IC 50 , 27.44 μg/mL, p  < 0.01) and tumour necrosis factor-alpha ( p  < 0.05) levels. Interleukin-1β ( p  < 0.05) and PGE 2 ( p  < 0.01) release decreased significantly with 25 μg/mL PINE. PINE 1 mg/ear inhibited LPS-stimulated expression of cyclooxygenase-2 and inducible NO synthase in RAW264.7 macrophages and significantly inhibited ear oedema (36.73-15.04% compared to the control, p  < 0.01) and MPO activity (167.94-105.59%, p  < 0.05).
Discussion and Conclusions: PINE exerts antioxidant and anti-inflammatory effects by inhibiting the production of inflammatory mediators. Identified flavonoids such as taxifolin and quercetin glucoside can be attributed to effect of PINE.

المؤلفون: Lee YJ; Cellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul, Republic of Korea., Kim JK; Department of Microbiology, Chungnam National University School of Medicine, Daejeon, Korea.; Department of Medical Science, Chungnam National University School of Medicine, Daejeon, Korea.; Infection Control Convergence Research Center, Chungnam National University School of Medicine, Daejeon, Korea., Jung CH; Cellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul, Republic of Korea., Kim YJ; Department of Microbiology, Chungnam National University School of Medicine, Daejeon, Korea.; Department of Medical Science, Chungnam National University School of Medicine, Daejeon, Korea.; Infection Control Convergence Research Center, Chungnam National University School of Medicine, Daejeon, Korea., Jung EJ; Cellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul, Republic of Korea., Lee SH; Cellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul, Republic of Korea., Choi HR; Cellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul, Republic of Korea., Son YS; Neuroscience Research Institute, Medical Research Center, College of Medicine, Seoul National University, Seoul, Republic of Korea., Shim SM; Cellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul, Republic of Korea., Jeon SM; Department of Microbiology, Chungnam National University School of Medicine, Daejeon, Korea.; Department of Medical Science, Chungnam National University School of Medicine, Daejeon, Korea.; Infection Control Convergence Research Center, Chungnam National University School of Medicine, Daejeon, Korea., Choe JH; Department of Microbiology, Chungnam National University School of Medicine, Daejeon, Korea.; Department of Medical Science, Chungnam National University School of Medicine, Daejeon, Korea.; Infection Control Convergence Research Center, Chungnam National University School of Medicine, Daejeon, Korea., Lee SH; Center for Research Equipment, Korea Basic Science Institute, Cheongju, Korea., Whang J; Korea Mycobacterium Resource Center (KMRC) & Basic Research Section, The Korean Institute of Tuberculosis (KIT), Cheongju, Korea., Sohn KC; Department of Medical Science, Chungnam National University School of Medicine, Daejeon, Korea.; Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea., Hur GM; Department of Medical Science, Chungnam National University School of Medicine, Daejeon, Korea.; Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea., Kim HT; Chemistry R&D Center, AUTOTAC Bio Inc, Seoul, Republic of Korea., Yeom J; Cellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul, Republic of Korea.; Department of Microbiology and Immunology, College of Medicine, Seoul National University, Seoul, Republic of Korea., Jo EK; Department of Microbiology, Chungnam National University School of Medicine, Daejeon, Korea.; Department of Medical Science, Chungnam National University School of Medicine, Daejeon, Korea.; Infection Control Convergence Research Center, Chungnam National University School of Medicine, Daejeon, Korea., Kwon YT; Cellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul, Republic of Korea.; Chemistry R&D Center, AUTOTAC Bio Inc, Seoul, Republic of Korea.; SNU Dementia Research Center, College of Medicine, Seoul National University, Seoul, Republic of Korea.; Ischemic/Hypoxic Disease Institute, College of Medicine, Seoul National University, Seoul, Republic of Korea.

المصدر: Autophagy [Autophagy] 2022 Dec; Vol. 18 (12), pp. 2926-2945. Date of Electronic Publication: 2022 Apr 05.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101265188 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-8635 (Electronic) Linking ISSN: 15548627 NLM ISO Abbreviation: Autophagy Subsets: MEDLINE

مواضيع طبية MeSH: Macroautophagy* , Autophagy*/genetics, Animals ; Mice ; Sequestosome-1 Protein/metabolism ; BCG Vaccine ; Ubiquitin/metabolism ; Apoptosis Regulatory Proteins/metabolism ; Salmonella typhimurium/metabolism ; Cytokines/metabolism ; Sirolimus/pharmacology

مستخلص: The N-degron pathway is a proteolytic system in which the N-terminal degrons (N-degrons) of proteins, such as arginine (Nt-Arg), induce the degradation of proteins and subcellular organelles via the ubiquitin-proteasome system (UPS) or macroautophagy/autophagy-lysosome system (hereafter autophagy). Here, we developed the chemical mimics of the N-degron Nt-Arg as a pharmaceutical means to induce targeted degradation of intracellular bacteria via autophagy, such as Salmonella enterica serovar Typhimurium ( S . Typhimurium), Escherichia coli , and Streptococcus pyogenes as well as Mycobacterium tuberculosis (Mtb). Upon binding the ZZ domain of the autophagic cargo receptor SQSTM1/p62 (sequestosome 1), these chemicals induced the biogenesis and recruitment of autophagic membranes to intracellular bacteria via SQSTM1, leading to lysosomal degradation. The antimicrobial efficacy was independent of rapamycin-modulated core autophagic pathways and synergistic with the reduced production of inflammatory cytokines. In mice, these drugs exhibited antimicrobial efficacy for S . Typhimurium, Bacillus Calmette-Guérin (BCG), and Mtb as well as multidrug-resistant Mtb and inhibited the production of inflammatory cytokines. This dual mode of action in xenophagy and inflammation significantly protected mice from inflammatory lesions in the lungs and other tissues caused by all the tested bacterial strains. Our results suggest that the N-degron pathway provides a therapeutic target in host-directed therapeutics for a broad range of drug-resistant intracellular pathogens. Abbreviations : ATG: autophagy-related gene; BCG: Bacillus Calmette-Guérin; BMDMs: bone marrow-derived macrophages; CALCOCO2/NDP52: calcium binding and coiled-coil domain 2; CFUs: colony-forming units; CXCL: C-X-C motif chemokine ligand; EGFP: enhanced green fluorescent protein; IL1B/IL-1β: interleukin 1 beta; IL6: interleukin 6; LIR: MAP1LC3/LC3-interacting region; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; Mtb: Mycobacterium tuberculosis ; MTOR: mechanistic target of rapamycin kinase; NBR1: NBR1 autophagy cargo receptor; OPTN: optineurin; PB1: Phox and Bem1; SQSTM1/p62: sequestosome 1; S . Typhimurium: Salmonella enterica serovar Typhimurium; TAX1BP1: Tax1 binding protein 1; TNF: tumor necrosis factor; UBA: ubiquitin-associated.

المؤلفون: Shal B; Pharmacological Sciences Research Lab, Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.; Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.; Faculty of Health Sciences, IQRA University, Islamabad Campus, (Chak Shahzad), Islamabad, Pakistan., Amanat S; Pharmacological Sciences Research Lab, Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.; Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan., Khan AU; Pharmacological Sciences Research Lab, Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.; Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.; Department of Pharmacy, Abasyn University, Peshawar, Pakistan., Lee YJ; College of Pharmacy and Inje Institute of Pharmaceutical Sciences and Research, Inje University, Gimhae, Gyeongnam, Republic of Korea., Ali H; Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan., Din FU; Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan., Park Y; College of Pharmacy and Inje Institute of Pharmaceutical Sciences and Research, Inje University, Gimhae, Gyeongnam, Republic of Korea., Khan S; Pharmacological Sciences Research Lab, Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.; Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

المصدر: Artificial cells, nanomedicine, and biotechnology [Artif Cells Nanomed Biotechnol] 2022 Dec; Vol. 50 (1), pp. 130-146.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 101594777 Publication Model: Print Cited Medium: Internet ISSN: 2169-141X (Electronic) Linking ISSN: 21691401 NLM ISO Abbreviation: Artif Cells Nanomed Biotechnol Subsets: MEDLINE

مواضيع طبية MeSH: Cystitis*/chemically induced , Cystitis*/drug therapy , Metal Nanoparticles*, Antioxidants ; Cyclophosphamide/adverse effects ; Gold/pharmacology ; Green Chemistry Technology/methods ; Humans ; Plant Extracts ; Polyethylene Glycols ; Tea

مستخلص: We investigated the effect of green tea extract PEGylated gold nanoparticles (P-AuNPs) making use of its targeted and sustained drug delivery against cyclophosphamide (CYP)-induced cystitis. AuNPs were synthesized by reduction reaction of gold salts with green tea extract following the concept of green synthesis. Mostly spherical-shaped P-AuNPs were synthesized with an average size of 14.3 ± 3.3 nm. Pre-treatment with P-AuNPs (1, 10 mg/kg, i.p.) before CYP (150 mg/kg, i.p.) challenge suggested its uroprotective properties. P-AuNPs significantly reversed all pain-like behaviours and toxicities produced by CYP resulting in a decreased aspartate aminotransferase, alanine aminotransferase, C-reactive protein, and creatinine level. P-AuNPs increased anti-oxidant system by increasing the level of reduced glutathione, glutathione-S-transferase, catalase and superoxide dismutase, and reduced nitric oxide production in bladder tissue. Additionally, it attenuated hypokalaemia and hyponatremia, along with a decrease in Evans blue content in bladder tissue and peritoneal cavity. CYP-induced bladder tissue damage observed by macroscopic and histological findings were remarkably attenuated by P-AuNPs, along with reduced fibrosis of collagen fibre in bladder smooth muscles shown by Masson's trichrome staining. Additionally, alterations in hematological parameters and clinical scoring were also prevented by P-AuNPs suggesting its uroprotective effect.

المؤلفون: Patil MP; Industry-University Cooperation Foundation, Pukyong National University, Busan, Republic of Korea., Kim JO; Department of Microbiology, Pukyong National University, Busan, Republic of Korea.; School of Marine and Fisheries Life Science, Pukyong National University, Busan, Republic of Korea., Lee YJ; Department of Marine Biology, Pukyong National University, Busan, Republic of Korea., Seo YB; Research Institute for Basic Science, Pukyong National University, Busan, Republic of Korea., Kim JK; Department of Marine Biology, Pukyong National University, Busan, Republic of Korea., Kim GD; Department of Microbiology, Pukyong National University, Busan, Republic of Korea.; School of Marine and Fisheries Life Science, Pukyong National University, Busan, Republic of Korea.

المصدر: Mitochondrial DNA. Part B, Resources [Mitochondrial DNA B Resour] 2022 Nov 23; Vol. 7 (11), pp. 2012-2014. Date of Electronic Publication: 2022 Nov 23 (Print Publication: 2022).

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 101676334 Publication Model: eCollection Cited Medium: Internet ISSN: 2380-2359 (Electronic) Linking ISSN: 23802359 NLM ISO Abbreviation: Mitochondrial DNA B Resour Subsets: PubMed not MEDLINE

مستخلص: The complete mitochondrial genome of the Chaetodon modestus (Temminck and Schlegel, 1844) was first determined in this study, which is 16,490 bp in length, containing 13 protein-coding genes, two rRNA genes, and 22 tRNA. Out of 37 mitochondrial genes, except for ND6 and eight tRNA ( Pro , Glu , Ser , Tyr , Cys , Asn , Ala , Gln ) genes were encoded on the L-strand, the others were encoded on the H-strand. The overall base composition includes A (28.0%), T (28.7%), G (16.7%), ad C (26.6%). The phylogenetic tree was built using the maximum-likelihood approach to provide a relationship within Chaetodontidae, which might be valuable for species management.
Competing Interests: No potential conflict of interest was reported by the author(s).
(© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)

المؤلفون: Patil MP; Industry-University Cooperation Foundation, Pukyong National University, Busan, Republic of Korea., Kim JO; Department of Microbiology, Pukyong National University, Busan, Republic of Korea.; School of Marine and Fisheries Life Science, Pukyong National University, Busan, Republic of Korea., Lee YJ; Department of Marine Biology, Pukyong National University, Busan, Republic of Korea., Seo YB; Research Institute for Basic Science, Pukyong National University, Busan, Republic of Korea., Kim JK; Department of Marine Biology, Pukyong National University, Busan, Republic of Korea., Kim GD; Department of Microbiology, Pukyong National University, Busan, Republic of Korea.; School of Marine and Fisheries Life Science, Pukyong National University, Busan, Republic of Korea.

المصدر: Mitochondrial DNA. Part B, Resources [Mitochondrial DNA B Resour] 2022 Nov 04; Vol. 7 (11), pp. 1922-1924. Date of Electronic Publication: 2022 Nov 04 (Print Publication: 2022).

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 101676334 Publication Model: eCollection Cited Medium: Internet ISSN: 2380-2359 (Electronic) Linking ISSN: 23802359 NLM ISO Abbreviation: Mitochondrial DNA B Resour Subsets: PubMed not MEDLINE

مستخلص: Chaetodon auriga (Forsskal, 1775) belongs to the family Chaetodontidae and the order Chaetodontiformes. Here, we report the complete mitochondrial genome of C. auriga assembled using the Illumina MiSeq platform. The circular mitochondrial genome of C. auriga is 16,527 bp long, has an A + T content of 54.53%, and contains 37 genes (13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes), and a non-coding region. The overall nucleotide composition was A: 28.19%, T: 26.34%, G: 16.27%, and C: 29.20%. The mitochondrial genome of C. auriga contributes to revealing the phylogenetic relationships among species of the Chaetodontidae family.
Competing Interests: No potential conflict of interest was reported by the author(s).
(© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)

المؤلفون: Kim HY; College of Pharmacy, Dankook University, Cheonan, Chungnam, Republic of Korea.; Center for Human Risk Assessment, Dankook University, Cheonan, Chungnam Republic of Korea., Lee YJ; College of Pharmacy, Dankook University, Cheonan, Chungnam, Republic of Korea., Kim SJ; College of Pharmacy, Dankook University, Cheonan, Chungnam, Republic of Korea., Lee JD; College of Pharmacy, Dankook University, Cheonan, Chungnam, Republic of Korea.; Center for Human Risk Assessment, Dankook University, Cheonan, Chungnam Republic of Korea., Kim S; Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan Republic of Korea., Ko MJ; Department Of Neuroscience, School Of Medicine, Konkuk University, Seoul, Republic of Korea., Kim JW; Department Of Neuroscience, School Of Medicine, Konkuk University, Seoul, Republic of Korea., Shin CY; Department Of Neuroscience, School Of Medicine, Konkuk University, Seoul, Republic of Korea., Kim KB; College of Pharmacy, Dankook University, Cheonan, Chungnam, Republic of Korea.; Center for Human Risk Assessment, Dankook University, Cheonan, Chungnam Republic of Korea.

المصدر: Journal of toxicology and environmental health. Part A [J Toxicol Environ Health A] 2022 Jan 02; Vol. 85 (1), pp. 1-13. Date of Electronic Publication: 2021 Aug 27.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 100960995 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1528-7394 (Print) Linking ISSN: 00984108 NLM ISO Abbreviation: J Toxicol Environ Health A Subsets: MEDLINE

مواضيع طبية MeSH: Disease Models, Animal*, Autism Spectrum Disorder/*metabolism , Valproic Acid/*toxicity, Animals ; Autism Spectrum Disorder/etiology ; Biomarkers/metabolism ; Brain/metabolism ; Female ; Male ; Maternal Exposure/adverse effects ; Metabolomics ; Proton Magnetic Resonance Spectroscopy ; Rats

مستخلص: Prenatal exposure to valproic acid (VPA) has been implicated in the manifestation of autism spectrum disorder (ASD)-like behavioral and functional changes both in human and rodents including mice and rats. The objective of this study was to determine metabolomics profiling and biomarkers related to VPA-induced symptoms resembling ASD using proton nuclear magnetic resonance (1H-NMR) spectral data. VPA was administered to pregnant rats at gestation day 12.5 and effects measured subsequently in male 4-week-old offspring pups. The sociability of VPA-treated animals was significantly diminished and exhibited ASD-like behavior as evidenced by reduction of social adaptation disorder and lack of social interactions. To find biomarkers related to ASD, the following were collected prefrontal brain cortices, urine bladder and blood samples directly from heart puncture. In all samples, principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) displayed significant clustering pattern differences between control and treated groups. Valine, taurine, myo-inositol, 3-hydroxybutyrate and 1,3-dihydroxyacetone were significantly decreased in brain cortices in treated rats. Serum metabolites of glucose, creatine phosphate, lactate, glutamine and threonine were significantly increased in VPA-administered animals. Urinary metabolites of pimelate, 3-hydroxyisovalerate and valerate were significantly reduced in VPA-treated rat, whereas galactose and galactonate levels were elevated. Various metabolites were associated with mitochondrial dysfunction metabolism and central nervous system disorders. Data demonstrated that VPA-induced alterations in endogenous metabolites of serum, urine, and brain cortex which might prove useful as biomarkers for symptoms resembling ASD as a model of this disorder.

المؤلفون: Kim JH; Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA, Jeonju, Korea., Park JS; College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea., Lee YJ; Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA, Jeonju, Korea., Choi S; Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA, Jeonju, Korea., Kim YH; College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea., Yang SY; College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.; Department of Pharmaceutical Engineering, Sangji University, Wonju-si, Republic of Korea.

المصدر: Journal of enzyme inhibition and medicinal chemistry [J Enzyme Inhib Med Chem] 2021 Dec; Vol. 36 (1), pp. 1049-1055.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 101150203 Publication Model: Print Cited Medium: Internet ISSN: 1475-6374 (Electronic) Linking ISSN: 14756366 NLM ISO Abbreviation: J Enzyme Inhib Med Chem Subsets: MEDLINE

مواضيع طبية MeSH: Enzyme Inhibitors/*pharmacology , Epoxide Hydrolases/*antagonists & inhibitors , Phytochemicals/*pharmacology , Plant Extracts/*pharmacology , Ulmus/*chemistry, Dose-Response Relationship, Drug ; Enzyme Inhibitors/chemistry ; Enzyme Inhibitors/isolation & purification ; Epoxide Hydrolases/metabolism ; Humans ; Molecular Docking Simulation ; Molecular Structure ; Phytochemicals/chemistry ; Phytochemicals/isolation & purification ; Plant Bark/chemistry ; Plant Extracts/chemistry ; Plant Extracts/isolation & purification ; Plant Roots/chemistry ; Solubility ; Structure-Activity Relationship

مستخلص: A novel compound 1 and nine known compounds ( 2 - 10 ) were isolated by open column chromatography analysis of the root bark of Ulmus davidiana . Pure compounds ( 1 - 10) were tested in vitro to determine the inhibitory activity of the catalytic reaction of soluble epoxide hydrolase (sEH). Compounds 1 , 2 , 4 , 6 - 8 , and 10 had IC 50 values ranging from 11.4 ± 2.3 to 36.9 ± 2.6 μM. We used molecular docking to simulate inhibitor binding of each compound and estimated the binding pose of the catalytic site of sEH. From this analysis, the compound 2 was revealed to be a potential inhibitor of sEH in vitro and in silico . Additionally, molecular dynamics (MD) study was performed to find detailed interaction signals of inhibitor 2 with enzyme. Finally, compound 2 is promising candidates for the development of a new sEH inhibitor from natural plants.