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المصدر: Animal Reproduction Science. 196:176-183
مصطلحات موضوعية: Male, 0301 basic medicine, endocrine system, Indoles, BOAR, Swine, Motility, Semen, macromolecular substances, digestive system, Cryopreservation, Andrology, Glycogen Synthase Kinase 3, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Food Animals, Freezing, Animals, Incubation, reproductive and urinary physiology, Sperm motility, 030219 obstetrics & reproductive medicine, Glycogen, urogenital system, Chemistry, General Medicine, Benzazepines, Spermatozoa, Sperm, 030104 developmental biology, Sperm Motility, Animal Science and Zoology, Semen Preservation
الوصف: Frozen-thawed boar sperm have less motility and fertility capacity in comparison to fresh sperm. Glycogen Synthase Kinase 3 (GSK3) contributes to sperm motility in fresh semen. In addition, GSK3 inhibition in boar spermatozoa in fresh semen improves motility variables. The role of GSK3 on boar cryopreserved sperm, however, is still unknown. The hypothesis in the present study was that GSK3 pathway inhibition by alsterpaullone (AST) could result in enhancement of the quality of sperm afer cryopreservation. Two different strategies were evaluated: i) AST supplementation to the freezing medium (AST + Cryo); ii) AST supplementation after sperm thawing (AST + Thaw). Sperm motility was evaluated using the CASA system and different sperm quality variables were evaluated using flow cytometry, as well as amount of GSK3 phosphorylation of thawed spermatozoa after 30 and 90 min incubation at 38.5 °C. Results indicate that AST supplementation had detrimental effects on sperm viability (live spermatozoa) and mitochondrial membrane potential when it was added after thawing (P 0.05) The AST supplementation after thawing, however, had a protective effect on plasma membrane lipid disorganization (P 0.05). The percentage of motile spermatozoa was not modified by AST supplementation. Nonetheless, after 30 min post-thawing, STR and LIN variables (related to straightness of the movement) as well as the percentage of rapid lineal spermatozoa were increased with both AST supplementation protocols. The GSK3α phosphorylation was not modified through the incubation time in boar thawed sperm. In summary, results do not support the idea of adding AST to the cryopreservation/thawing medium to improve boar sperm quality after cryopreservation.
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المصدر: Reproduction in domestic animals = Zuchthygiene. 53(3)
مصطلحات موضوعية: 0301 basic medicine, Male, endocrine system, endocrine system diseases, BOAR, Sus scrofa, Motility, law.invention, Andrology, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, law, Refrigeration, medicine, Animals, Acrosome, reproductive and urinary physiology, Sperm motility, Membrane Potential, Mitochondrial, 030219 obstetrics & reproductive medicine, urogenital system, Chemistry, Extender, nutritional and metabolic diseases, Semen cryopreservation, Sperm, Spermatozoa, Metformin, Semen Analysis, 030104 developmental biology, Sperm Motility, Animal Science and Zoology, Biotechnology, medicine.drug, Semen Preservation
الوصف: Metformin is clinically used to treat diabetes. Given its role-impacting metabolism, metformin has been also added to semen cryopreservation media showing specie-dependent effects. We aimed to investigate metformin effects in both fresh (38.5°C for 2, 24 hr) and refrigerated (17°C for 10 days) boar spermatozoa. Metformin (2 hr) does not affect fresh sperm viability, membrane lipid organization nor acrosome integrity. However, metformin (24 hr) blocks sperm ΔΨm and significantly reduces % motile spermatozoa (65%), % progressive spermatozoa (50%), % rapid (100%), velocities VCL (69%), VSL (86%), VAP (78%) and motility coefficients. Metformin-including extender does not modify sperm viability, membrane lipid organization or acrosome integrity. Furthermore, it significantly reduces high ΔΨ-population spermatozoa at refrigeration day 4. Metformin also significantly reduces sperm motility during refrigeration. Summarizing, metformin inhibits both boar sperm ΔΨ and motility in any sperm condition studied: fresh and refrigerated. These findings dissuade metformin as an additive to improve boar sperm quality.
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المؤلفون: Hoi Chang Lee, Luis Aguila, Pablo E. Visconti, Ricardo Felmer, Felipe Navarrete, Rafael A. Fissore, María Elena Arias, David Martin-Hidalgo
مصطلحات موضوعية: 0301 basic medicine, Male, Embryology, medicine.medical_treatment, Acrosome reaction, Parthenogenesis, Heterologous, Cattle Diseases, Biology, Intracytoplasmic sperm injection, Article, Andrology, Embryo Culture Techniques, 03 medical and health sciences, Mice, Endocrinology, Human fertilization, Species Specificity, Capacitation, medicine, Animals, Calcium Signaling, Sperm Injections, Intracytoplasmic, reproductive and urinary physiology, Cells, Cultured, Infertility, Male, Cell Nucleus, Sperm-Ovum Interactions, urogenital system, Obstetrics and Gynecology, Oocyte activation, Cell Biology, Chromatin Assembly and Disassembly, In vitro maturation, In Vitro Oocyte Maturation Techniques, 030104 developmental biology, Reproductive Medicine, Sperm Head, Cattle, Female, Sperm Capacitation
الوصف: The efficiency of intracytoplasmic sperm injection (ICSI) in the bovine is low compared to other species. It is unknown whether defective oocyte activation and/or sperm head decondensation limit the success of this technique in this species. To elucidate where the main obstacle lies, we used homologous and heterologous ICSI and parthenogenetic activation procedures. We also evaluated whetherin vitromaturation negatively impacted the early stages of activation after ICSI. Here we showed that injected bovine sperm are resistant to nuclear decondensation by bovine oocytes and this is only partly overcome by exogenous activation. Remarkably, when we used heterologous ICSI,in vivo-matured mouse eggs were capable of mounting calcium oscillations and displaying normal PN formation following injection of bovine sperm, althoughin vitro-matured mouse oocytes were unable to do so. Together, our data demonstrate that bovine sperm are especially resistant to nuclear decondensation byin vitro-matured oocytes and this deficiency cannot be simply overcome by exogenous activation protocols, even by inducing physiological calcium oscillations. Therefore, the inability of a suboptimal ooplasmic environment to induce sperm head decondensation limits the success of ICSI in the bovine. Studies aimed to improve the cytoplasmic milieu ofin vitro-matured oocytes and to replicate the molecular changes associated within vivocapacitation and acrosome reaction will deepen our understanding of the mechanism of fertilization and improve the success of ICSI in this species.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0eec2daf6051b9a730f42aefa0b717c7
https://europepmc.org/articles/PMC6430150/
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