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المصدر: Food chemistry. 355
مصطلحات موضوعية: Time Factors, Glutens, medicine.drug_class, Consumer awareness, Monoclonal antibody, digestive system, 01 natural sciences, Gliadin, Analytical Chemistry, 0404 agricultural biotechnology, Food Labeling, Limit of Detection, medicine, Humans, Food science, Food allergens, chemistry.chemical_classification, Immunoassay, biology, digestive, oral, and skin physiology, 010401 analytical chemistry, food and beverages, nutritional and metabolic diseases, Antibodies, Monoclonal, 04 agricultural and veterinary sciences, General Medicine, 040401 food science, Gluten, digestive system diseases, 0104 chemical sciences, Food labeling, Product availability, chemistry, biology.protein, Gold, Food Analysis, Food Science, Lateral flow immunoassay
الوصف: The gluten protein found in a variety of cereal grains is a food allergen that can elicit a spectrum of immuno-inflammatory responses in people. Consumer awareness has prompted changes in food labeling requirements, expanded gluten-free food product availability and increased demand for effective gluten testing methodologies. To meet the challenges associated with gluten testing from diverse and complex foods we developed a lateral flow immunoassay (LFIA) using a pair of novel gliadin monoclonal antibodies (MAbs). Using a visual gold reporter, we show sensitive gluten detection (150 ng/mL) from complex food substrates using a fast (5 min) and easy testing methodology. In this report we characterize the binding properties of a cohort of newly generated gliadin monoclonal antibodies suitable for gluten detection using multiple assay formats and introduce a novel plug-n-play test strip platform with integrated test components in a single-use format.
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المؤلفون: Alzbeta Stara, Anna Koubová, Josef Priborsky, Lenka Schebestova, Josef Velisek, Helena Curdova, Zdenka Svobodova, Alena Honzlova, Pavel Bartak
المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Scientific Reportsمصطلحات موضوعية: Fish Proteins, Veterinary medicine, Carps, Fat content, Nitrogen, Science, Fisheries, chemistry.chemical_element, Food Contamination, 01 natural sciences, Article, Cyprinus, Fats, Common carp, 0404 agricultural biotechnology, Food Labeling, Fish Products, Animals, Dry matter, Fillet (mechanics), Czech Republic, Multidisciplinary, biology, 010401 analytical chemistry, 04 agricultural and veterinary sciences, Consumer protection, biology.organism_classification, Chemical biology, 040401 food science, 0104 chemical sciences, chemistry, Freshwater fish, Freshwater ecology, Medicine, sense organs, Food Analysis
الوصف: Consumer protection against food adulteration and misleading labelling is integrated into EU legislation, but accurate analysis of the meat content of farmed freshwater fish products is not possible because of the lack of established nitrogen factors for farmed common carp. The aim of this study was to determine nitrogen factors for farmed common carp Cyprinus carpio. Seven-hundred samples collected in 2018–2019 in three harvest seasons (March/April, Jun/July, and October/November) at seven locations in the Czech Republic were analysed for nitrogen, dry matter, protein, ash, and fat content according to standard ISO methods. The recommended nitrogen factor for fat-free common carp fillet with skin is 3.04 ± 0.13 and, for fillet without skin, 2.95 ± 0.12. Availability of nitrogen factors for common carp can help ensure that consumers are purchasing correctly labelled products.
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المؤلفون: Tune Wulff, André M. Deelder, D. Ohana, Jonas Bergquist, Hans Dalebout, Magnus Palmblad, Rob J. Marissen
المصدر: Food Chemistry, 203, 28-34. ELSEVIER SCI LTD
مصطلحات موضوعية: Proteomics, Identification, Swine, Spectral libraries, Analytical chemistry, Shotgun, Biology, 01 natural sciences, Poultry, Spectral line, Quantitation, Analytical Chemistry, 0404 agricultural biotechnology, Food Labeling, Tandem Mass Spectrometry, Genus, Phylogenetics, Shotgun proteomics, Animals, Phylogeny, Phylogenetic tree, business.industry, 010401 analytical chemistry, Pattern recognition, Ruminants, 04 agricultural and veterinary sciences, General Medicine, 040401 food science, 0104 chemical sciences, Meat Products, Food products, Spectral matching, Identification (biology), Artificial intelligence, Peptides, business, Food Analysis, Chromatography, Liquid, Food Science
الوصف: A new method, based on shotgun spectral matching of peptide tandem mass spectra, was successfully applied to the identification of different food species. The method was demonstrated to work on raw as well as processed samples from 16 mammalian and 10 bird species by counting spectral matches to spectral libraries in a reference database with one spectral library per species. A phylogenetic tree could also be constructed directly from the spectra. Nearly all samples could be correctly identified at the species level, and 100% at the genus level. The method does not use any genomic information and unlike targeted methods, no prior knowledge of genetic variation within a genus or species is necessary. (c) 2016 Elsevier Ltd. All rights reserved.
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المؤلفون: Fuki Kawaguchi, Hiroaki Goto, Shinji Sasazaki, Yoshikazu Yamamoto, Ryuji Nakajima, Yuichi Washida, Hideyuki Mannen, Masayoshi Takahashi, Yuto Kitamura
المصدر: Animal Science Journal. 89:257-258
مصطلحات موضوعية: Genetic Markers, 0301 basic medicine, Meat, Combined use, Food Contamination, Breeding, Biology, DNA, Mitochondrial, Japanese Black cattle, 03 medical and health sciences, Animal science, Gene Frequency, Japan, Food Labeling, Animals, Australian Wagyu, DNA marker, Allele frequency, Australia, 0402 animal and dairy science, food and beverages, misbranded beef, 04 agricultural and veterinary sciences, General Medicine, 040201 dairy & animal science, Breed, Food labeling, 030104 developmental biology, Genetic marker, Cattle, deterrent force, General Agricultural and Biological Sciences, Food Analysis
الوصف: The objective of this study was to discriminate between original Japanese and Australian Wagyu beef, which is sold in the Singapore markets, using six previously developed DNA markers. To effectively evaluate the six markers for breed identification, the probability of identification as Australian Wagyu beef was calculated based on the estimated allele frequencies using 130 Australian Wagyu individuals. The combined use of six markers would allow the discrimination of Australian Wagyu beef with an estimated probability of 0.776. The probability to discriminate Australian Wagyu from Japanese Wagyu beef was sufficiently high. In addition, Australian Wagyu has maternal mitochondrial DNA of Bos indicus cattle with moderate high frequency of 0.377. The DNA marker system could also be used as a deterrent force against false sales, and contribute to the reduction and prevention of incorrect or falsified labeling of beef.
وصف الملف: application/pdf
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المؤلفون: Jordi Viñas, Ana Gordoa, Gustavo Carreras, Nuria Sanz
المصدر: PLoS ONE
Recercat. Dipósit de la Recerca de Catalunya
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PLoS One, 2017, vol. 12, núm. 1, p.e0170809
Articles publicats (D-B)
DUGiDocs – Universitat de Girona
PLoS ONE, Vol 12, Iss 1, p e0170809 (2017)
Digital.CSIC. Repositorio Institucional del CSICمصطلحات موضوعية: 0106 biological sciences, Economics, Physiology, Gene Identification and Analysis, lcsh:Medicine, Social Sciences, 01 natural sciences, Biochemistry, Tonyina -- Etiquetatge, Database and Informatics Methods, Food Labeling, Medicine and Health Sciences, lcsh:Science, Tonyina -- Indústria i comerç, Multidisciplinary, Fish market, biology, Fraud, Fishes, Commerce, 04 agricultural and veterinary sciences, 040401 food science, Mitochondrial DNA, Nucleic acids, Geography, Physiological Parameters, Osteichthyes, Vertebrates, Sequence Analysis, Thunnus, Research Article, Deception, Forms of DNA, Shops, Bioinformatics, Fishing, Fisheries, Sequence Databases, Research and Analysis Methods, 0404 agricultural biotechnology, Tuna -- Labeling, Genetics, Animals, Obesity, Regulations, Behavior, Tuna, 010604 marine biology & hydrobiology, lcsh:R, Substitution (logic), Retail, Body Weight, Organisms, Biology and Life Sciences, DNA, Tuna -- Grain trade, biology.organism_classification, Illegal fishing, Food labeling, Fishery, Biological Databases, Seafood, Spain, lcsh:Q, Law and Legal Sciences, Food Analysis
الوصف: Este artículo contiene 15 páginas, 4 tablas, 3 figuras.
Intentional mislabelling of seafood is a widespread problem, particularly with high-value species like tuna. In this study we examine tuna mislabelling, deliberate species substitution, types of substitution and its impact on prices. The survey covered the commercial chain, from Merca-Barna to fishmongers and restaurants in the Spanish Autonomous Community of Catalonia. To understand the geographic extent of the problem we also sampled Merca- Madrid, Europe's biggest fish market, and Merca-MaÂlaga for its proximity to the bluefin tuna migratory route and trap fishery. Monthly surveys were carried out over one year. The results showed a high deficiency in labelling: 75% of points of sale and 83% of restaurants did not specify the species, and in those cases the name of the species had to be asked. A total of 375 samples were analysed genetically, the largest dataset gathered in Europe so far. The identified species were Thunnus albacares, Thunnus thynnus and Thunnus obesus. Species substitution began at suppliers, with 40% of observed cases, increasing to 58% at fishmongers and 62% at restaurants. The substitution was mainly on bluefin tuna (T. thynnus), 73% of cases. At restaurants, only during the bluefin fishing season, we observed a decrease of Bluefin tuna substitution and an increase of reverse substitution revealing some illegal fishing. The effect of species substitution on species prices was relevant: T. obesus increased its price by around €12 kg-1 when it was sold as bluefin. In view of the deficiency of labelling, the abuse of generic names and the lack of the bluefin catch document, we conclude that the Spanish regulations are ineffective, highlighting the need for policy execution, and the urgent need for information campaigns to Spanish consumers.
The study was funded by the Balfego Group under a programme of scientific cooperation with the Blanes Centre for Advanced Study (CEAB - CSIC).وصف الملف: application/pdf
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المؤلفون: Isabel González, Luis Asensio, Miguel Ángel Pavón, Teresa García, Rosario Martín
المصدر: Food Additives & Contaminants: Part A. 25:677-683
مصطلحات موضوعية: Indirect elisa, Veterinary medicine, Health, Toxicology and Mutagenesis, Enzyme-Linked Immunosorbent Assay, Food Contamination, Toxicology, Polymerase Chain Reaction, 01 natural sciences, law.invention, 0404 agricultural biotechnology, Species Specificity, Food Labeling, law, Multiplex polymerase chain reaction, Animals, Grouper, Polymerase chain reaction, Fish market, biology, 010401 analytical chemistry, Fishes, Public Health, Environmental and Occupational Health, Epinephelus marginatus, Fish fillet, 04 agricultural and veterinary sciences, General Chemistry, General Medicine, biology.organism_classification, 040401 food science, Molecular biology, 0104 chemical sciences, Seafood, Food Analysis, Control methods, Food Science
الوصف: An indirect enzyme-linked immunosorbent assay (ELISA) and a multiplex polymerase chain reaction (PCR) procedure was applied for the detection of Grouper (Epinephelus marginatus) mislabelling in the fish market. An indirect ELISA (microtiter-plate format) using two monoclonal antibodies (3D12 and 1A4) was assayed and multiplex PCR performed using species-specific primers of the 5S rDNA gene for the rapid authentication of grouper. A total of 70 commercial fish fillet samples, collected from local markets and supermarkets, labelled as grouper were analysed: 12 of the 70 samples were confirmed to be Grouper. The PCR technique permitted the detection of Nile Perch (Lates niloticus) in the commercial fillet samples, which was not possible using ELISA. The results suggest that both ELISA and PCR are specific and reliable tools for the detection of Grouper mislabelling/adulteration and the accurate implementation of traceability for successful regulatory food controls.
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