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المؤلفون: Dan Li, Zhangjian Li, Yang Jiao, Chen Yang, Yiwen Xu, Zhitian Shen, Hongtao Ma, Weiwei Shao, Shi Wei, Yaoyao Cui
المصدر: BioMed Research International, Vol 2020 (2020)
BioMed Research Internationalمصطلحات موضوعية: Male, 0301 basic medicine, Materials science, Focus (geometry), Article Subject, Neocortex, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Imaging phantom, Rats, Sprague-Dawley, 03 medical and health sciences, Neural activity, Seizures, Photoplethysmogram, 0103 physical sciences, medicine, Animals, Humans, 010301 acoustics, Electrocorticography, Epilepsy, General Immunology and Microbiology, medicine.diagnostic_test, General Medicine, Electrodes, Implanted, Rats, 030104 developmental biology, Cerebral blood volume, Transducer, Medicine, sense organs, Neurovascular coupling, Research Article, Biomedical engineering
الوصف: The cryptogenic epilepsy of the neocortex is a disease in which the seizure is accompanied by intense cerebral nerve electrical activities but the lesions are not observed. It is difficult to locate disease foci. Electrocorticography (ECoG) is one of the gold standards in seizure focus localization. This method detects electrical signals, and its limitations are inadequate resolution which is only 10 mm and lack of depth information. In order to solve these problems, our new method with implantable micro ultrasound transducer (MUT) and photoplethysmogram (PPG) device detects blood changes to achieve higher resolution and provide depth information. The basis of this method is the neurovascular coupling mechanism, which shows that intense neural activity leads to sufficient cerebral blood volume (CBV). The neurovascular coupling mechanism established the relationship between epileptic electrical signals and CBV. The existence of mechanism enables us to apply our new methods on the basis of ECoG. Phantom experiments and in vivo experiments were designed to verify the proposed method. The first phantom experiments designed a phantom with two channels at different depths, and the MUT was used to detect the depth where the blood concentration changed. The results showed that the MUT detected the blood concentration change at the depth of 12 mm, which is the position of the second channel. In the second phantom experiments where a PPG device and MUT were used to monitor the change of blood concentration in a thick tube, the results showed that the trend of superficial blood concentration change provided by the PPG device is the same as that provided by the MUT within the depth of 2.5 mm. Finally, in the verification of in vivo experiments, the blood concentration changes on the surface recorded by the PPG device and the changes at a certain depth recorded by the MUT all matched the seizure status shown by ECoG. These results confirmed the effectiveness of the combined micro sensors.
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المؤلفون: Hong Li, Xiaoyan Shen, Weiwei Shao, Shaorui Chen, Chun Zhou, Cuixian Li, Jin Peng, Dong Li, Yunzi Ma, Yang Yu, Zhongbin Cheng, Sheng Yin, Yan You, Peiqing Liu
المصدر: Translational Research. 163:160-170
مصطلحات موضوعية: MAPK/ERK pathway, medicine.medical_specialty, Heart Diseases, MAP Kinase Signaling System, Cardiac fibrosis, SMAD, Acetates, Transforming Growth Factor beta, Fibrosis, Physiology (medical), Internal medicine, medicine, Animals, biology, Plant Extracts, Biochemistry (medical), Public Health, Environmental and Occupational Health, General Medicine, Transforming growth factor beta, Aristolochia, medicine.disease, Angiotensin II, Rats, Endocrinology, cardiovascular system, biology.protein, Myocardial fibrosis, Signal Transduction, Transforming growth factor
الوصف: Aristolochia yunnanensis, known as Nan Mu Xiang in traditional Chinese medicine, has long been used to treat hypertension and chest pain. In this study, the effect of ethyl acetate extract of Nan Mu Xiang (NMX) on cardiac fibrosis was assessed in vitro by cultured adult rat cardiac fibroblasts with angiotensin II (AngII) stimulation, and in vivo by rats with abdominal aorta constriction (AAC). In cultured adult rat cardiac fibroblasts stimulated by AngII, NMX inhibited cardiac fibroblast proliferation, reduced the expression of fibronectin, α-smooth muscle actin (α-SMA), and transforming growth factor β (TGF-β) in a dose-dependent manner; and suppressed AngII-induced phosphorylation of extracellular signal-regulated kinase (ERK)1/2, C- rapidly accelerated fibrosarcoma (C-Raf), and small mother against decapentaplegic (Smad) 2. Similar results were also observed in AAC rats with intraperitoneal injection of NMX, which not only ameliorated myocardial fibrosis, but also improved cardiac function. The therapeutic effect of NMX on myocardial fibrosis is attributed mainly to the inhibition of ERK and the TGF-β/Smad signaling pathways. NMX may be a promising potential drug candidate for myocardial fibrosis.
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المؤلفون: Jin Peng, Ying Wang, Liang Lu, Weiwei Shao, Xiaoyan Shen, Xin-Wen Zhang, Yan You, Dong Li, Chun Zhou, Cuixian Li
المصدر: Molecular and Cellular Biochemistry. 381:273-282
مصطلحات موضوعية: STAT3 Transcription Factor, Clinical Biochemistry, Biology, S Phase, Downregulation and upregulation, Cell Line, Tumor, Survivin, Animals, Humans, Phosphorylation, RNA, Small Interfering, Phosphotyrosine, STAT3, Molecular Biology, Cell Proliferation, Gene knockdown, Brain Neoplasms, Cell growth, G1 Phase, Glioma, Cell Biology, General Medicine, Phenanthrenes, Rats, nervous system diseases, Cell culture, STAT protein, Cancer research, biology.protein, Mutant Proteins, Signal Transduction
الوصف: Malignant gliomas (MGs) are among the most aggressive types of cancers in the human brain. Frequent tumor recurrence caused by a lack of effective therapeutic approaches results in a poor prognosis. Signal transducer and activator of transcription 3 (STAT3), an oncogenic protein, is constitutively activated in MGs and predicts a poor clinical outcome. STAT3 therefore is considered to be a promising target for the treatment of MGs. Cryptotanshinone (CTS), the main bioactive compound from the root of Salvia miltiorrhiza Bunge, has been reported to have various pharmacological effects. However, little is known about its function in MG cells. In this study, we evaluated the effect of CTS on the proliferation of human glioma cell lines (T98G and U87). Our results revealed that CTS significantly suppresses glioma cell proliferation. The phosphorylation of STAT3 Tyr705, but not Ser727, was inhibited by CTS, and STAT3 nuclear translocation was attenuated. Overexpression of constitutively active mutant STAT3C reversed the inhibitory effect of CTS, while knockdown STAT3 showed a similar inhibitory effect as CTS treatment. Following the downregulation of STAT3-regulated proteins cyclinD1 and survivin, cell cycle progression significantly arrested in G1/G0 phase. These results indicate that CTS may be a potential antiproliferation agent for the treatment of MGs and that its mechanism may be related to the inhibition of STAT3 signaling.
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المؤلفون: Chen Lin, Pengcheng Yan, Weiwei Shao, Lei Yue, Xi-Yan Ma, Jianyong Dong, Xiao-Qin Cai
المصدر: Phytochemistry. 85
مصطلحات موضوعية: Magnetic Resonance Spectroscopy, Stereochemistry, Plant Science, Horticulture, Biochemistry, Antiviral Agents, Virus, Cell Line, Sesquiterpenes, Guaiane, Curcuma wenyujin, Curcuma, Dogs, Influenza, Human, Animals, Humans, Molecular Biology, Polycyclic Sesquiterpenes, biology, Molecular Structure, Chemistry, General Medicine, Nuclear magnetic resonance spectroscopy, biology.organism_classification, In vitro, Rhizome, Zingiberaceae, Two-dimensional nuclear magnetic resonance spectroscopy, Sesquiterpenes
الوصف: Five sesquiterpenoids, 1α,8α-epidioxy-4α-hydroxy- 5αH-guai-7(11),9-dien- 12,8-olide. (1), 8,9-seco-4β-hydroxy-1α,5βH-7(11)-guaen-8,10-olide (2), 8α-hydroxy-1α, 4β,7βH-guai-10(15)-en- 5β,8β-endoxide(3), 7β,8α-dihydroxy-1α,4αH-guai-10(15)-en-5β,8β-endoxide(4) and 7-hydroxy-5(10),6,8-cadinatriene-4-one(5), together with seven known analogs were isolated from the rhizomes of Curcuma wenyujin. Their structures and relative configurations were determined on the basis of spectroscopic methods including 2D NMR techniques, and the structures of 1 and 2 were confirmed by single-crystal X-ray diffraction experiment. Compounds 1-10 and 12 showed significant in vitro antiviral activity against the influenza virus A with IC₅₀ values ranged from 6.80 to 39.97 μM, and SI values ranged from 6.35 to 37.25.
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