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1دورية أكاديمية
المؤلفون: Jayaswal, Vivek, Ndo, Cyrille, Ma, Hsiu-Ching, Clifton, Bryan D, Pombi, Marco, Cabrera, Kevin, Couhet, Anna, Mouline, Karine, Diabaté, Abdoulaye, Dabiré, Roch, Ayala, Diego, Ranz, José M
المصدر: Genome Biology and Evolution. 13(9)
مصطلحات موضوعية: Human Genome, Biotechnology, Infectious Diseases, Genetics, Good Health and Well Being, Animals, Anopheles, Female, Insecticide Resistance, Insecticides, Malaria, Male, Transcriptome, transcriptome variation, functional diversification, sex-biased gene expression, faster-X effect, Anopheles arabiensis, Anopheles arabiensis, Biochemistry and Cell Biology, Evolutionary Biology, Developmental Biology
الوصف: The magnitude and functional patterns of intraspecific transcriptional variation in the anophelines, including those of sex-biased genes underlying sex-specific traits relevant for malaria transmission, remain understudied. As a result, how changes in expression levels drive adaptation in these species is poorly understood. We sequenced the female, male, and larval transcriptomes of three populations of Anopheles arabiensis from Burkina Faso. One-third of the genes were differentially expressed between populations, often involving insecticide resistance-related genes in a sample type-specific manner, and with the females showing the largest number of differentially expressed genes. At the genomic level, the X chromosome appears depleted of differentially expressed genes compared with the autosomes, chromosomes harboring inversions do not exhibit evidence for enrichment of such genes, and genes that are top contributors to functional enrichment patterns of population differentiation tend to be clustered in the genome. Further, the magnitude of variation for the sex expression ratio across populations did not substantially differ between male- and female-biased genes, except for some populations in which male-limited expressed genes showed more variation than their female counterparts. In fact, female-biased genes exhibited a larger level of interpopulation variation than male-biased genes, both when assayed in males and females. Beyond uncovering the extensive adaptive potential of transcriptional variation in An. Arabiensis, our findings suggest that the evolutionary rate of changes in expression levels on the X chromosome exceeds that on the autosomes, while pointing to female-biased genes as the most variable component of the An. Arabiensis transcriptome.
وصف الملف: application/pdf
URL الوصول: https://escholarship.org/uc/item/79x6f1kx
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2دورية أكاديمية
المؤلفون: Bonnet, Emmanuel, Fournet, Florence, Benmarhnia, Tarik, Ouedraogo, Samiratou, Dabiré, Roch, Ridde, Valéry
المصدر: Infectious Diseases of Poverty. 9(1)
مصطلحات موضوعية: Infectious Diseases, Vector-Borne Diseases, Prevention, 3.1 Primary prevention interventions to modify behaviours or promote wellbeing, Prevention of disease and conditions, and promotion of well-being, Good Health and Well Being, Aedes, Animal Distribution, Animals, Burkina Faso, Mosquito Control, Mosquito Vectors, Residence Characteristics, Seasons, Spatial Analysis, Vector-borne diseases, Spatial analysis, Community-based intervention, Clinical Sciences, Medical Microbiology, Public Health and Health Services
الوصف: BACKGROUND:Several studies highlighted the impact of community-based interventions whose purpose was to reduce the vectors' breeding sites. These strategies are particularly interesting in low-and-middle-income countries which may find it difficult to sustainably assume the cost of insecticide-based interventions. In this case study we determine the spatial distribution of a community-based intervention for dengue vector control using different entomological indices. The objective was to evaluate locally where the intervention was most effective, using spatial analysis methods that are too often neglected in impact assessments. METHODS:Two neighbourhoods, Tampouy and Juvenat in Ouagadougou, Burkina Faso, were chosen among five after a survey was conducted, as part of an assessment related to the burden of dengue. As part of the community-based intervention conducted in Tampouy between August and early October 2016, an entomological survey was implemented in two phases. The first phase consisted of a baseline entomological characterization of potential breeding sites in the neighbourhood of Tampouy as well as in Juvenat, the control area. This phase was conducted in October 2015 at the end of the rainy season. The mosquito breeding sites were screened in randomly selected houses: 206 in Tampouy and 203 in Juvenat. A second phase took place after the intervention, in October 2016. The mosquito breeding sites were investigated in the same yards as during the baseline phase. We performed several entomological analyses to measure site productivity as well as before and after analysis using multilevel linear regression. We used Local Indicators of Spatial Association (LISAs) to analyse spatial concentrations of larvae. RESULTS:After the intervention, it is noted that LISAs at Tampouy reveal few aggregates of all types and the suppression of those existing before the intervention. The analysis therefore reveals that the intervention made it possible to reduce the number of concentration areas of high and low values of pupae. CONCLUSIONS:The contribution of spatial methods for assessing community-based intervention are relevant for monitoring at local levels as a complement to epidemiological analyses conducted within neighbourhoods. They are useful, therefore, not only for assessment but also for establishing interventions. This study shows that spatial analyses also have their place in population health intervention research.
وصف الملف: application/pdf
URL الوصول: https://escholarship.org/uc/item/1wg7b09n
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3دورية أكاديمية
المؤلفون: Ouédraogo, Samiratou, Benmarhnia, Tarik, Bonnet, Emmanuel, Somé, Paul-André, Barro, Ahmed S, Kafando, Yamba, Soma, Diloma Dieudonné, Dabiré, Roch K, Saré, Diane, Fournet, Florence, Ridde, Valéry
المصدر: Emerging Infectious Diseases. 24(10)
مصطلحات موضوعية: Biomedical and Clinical Sciences, Public Health, Health Sciences, Clinical Sciences, Emerging Infectious Diseases, Clinical Research, Rare Diseases, Prevention, Infectious Diseases, Vector-Borne Diseases, Clinical Trials and Supportive Activities, Infection, Good Health and Well Being, Animals, Burkina Faso, Community Health Services, Dengue, Dengue Virus, Disease Vectors, Epidemiologic Research Design, Geography, Humans, Mosquito Control, Mosquito Vectors, Outcome Assessment, Health Care, Preventive Health Services, Aedes aegypti, arbovirus, community-based, dengue, effectiveness, intervention, mosquitoes, sub-Saharan Africa, vector-borne disease, viruses, Medical Microbiology, Public Health and Health Services, Microbiology, Clinical sciences, Epidemiology, Health services and systems
الوصف: We evaluated the effectiveness of a community-based intervention for dengue vector control in Ouagadougou, the capital city of Burkina Faso. Households in the intervention (n = 287) and control (n = 289) neighborhoods were randomly sampled and the outcomes collected before the intervention (October 2015) and after the intervention (October 2016). The intervention reduced residents' exposure to dengue vector bites (vector saliva biomarker difference -0.08 [95% CI -0.11 to -0.04]). The pupae index declined in the intervention neighborhood (from 162.14 to 99.03) and increased in the control neighborhood (from 218.72 to 255.67). Residents in the intervention neighborhood were less likely to associate dengue with malaria (risk ratio 0.70 [95% CI 0.58-0.84]) and had increased knowledge about dengue symptoms (risk ratio 1.44 [95% CI 1.22-1.69]). Our study showed that well-planned, evidence/community-based interventions that control exposure to dengue vectors are feasible and effective in urban settings in Africa that have limited resources.
وصف الملف: application/pdf
URL الوصول: https://escholarship.org/uc/item/3t32f46d
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المؤلفون: Olivier Roux, Roch K. Dabiré, Abdoulaye Diabaté, Laurent Dormont, Benoit Lapeyre, Serge Bèwadéyir Poda, Olivier Gnankiné, Bruno Buatois
المصدر: Nature Ecology & Evolution. 6:1676-1686
مصطلحات موضوعية: Male, biology, Ecology, Anopheles gambiae, Swarming (honey bee), Swarm behaviour, Zoology, biology.organism_classification, medicine.disease, Pheromones, Malaria, Chemical ecology, Sexual Behavior, Animal, Sibling species, Sex pheromone, Anopheles, medicine, Animals, Female, Sex Attractants, Ecology, Evolution, Behavior and Systematics
الوصف: Cues involved in mate seeking and recognition prevent hybridization and can be involved in speciation processes. In malaria mosquitoes, females of the two sibling species Anopheles gambiae s.s. and An. coluzzii mate in monospecific male swarms and hybrids are rare. Long-range sex pheromones driving this behavior have been debated in literature but to date, no study has proven their existence or their absence. Here, we attempted to bring to light their existence. To put all the odds in our favor, we used different chemical ecology methods such as behavioral and electrophysiological assays as well chemical analyses, and we worked with mosquitoes at their optimal physiological mating state i.e. with swarming males during their natural swarming windows. Despite all our efforts, our results support the absence of long-range sex pheromones involved in swarm detection and recognition by females. We briefly discuss the implications of this finding in ecology, evolution and for control strategies.
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المؤلفون: Martin Bienvenu Somda, Jacques Kaboré, Sheila Médina Karambiri, Emilie Dama, Der Dabiré, Charlie Franck Alfred Compaoré, Ernest Wendemanedgé Salou, Hamidou Ilboudo, Isidore Houaga, Fabrice Courtin, Adrien Marie Gaston Belem, Vincent Jamonneau, Zakaria Bengaly
المصدر: Somda, M B, Kaboré, J, Karambiri, S M, Dama, E, Dabiré, D, Compaoré, C F A, Salou, E W, Ilboudo, H, Houaga, I, Courtin, F, Belem, A M G, Jamonneau, V & Bengaly, Z 2022, ' Evaluation of the Re-emergence Risk of Human African Trypanosomiasis in the Southwestern Burkina Faso, A Gold-Bearing Mutation Area ', Acta Parasitologica, vol. 67, no. 2, pp. 714-722 . https://doi.org/10.1007/s11686-021-00512-2
مصطلحات موضوعية: Trypanosoma, Trypanosomiasis, African, Swine, Mutation, Burkina Faso, Human African trypanosomiasis, Risk of re-emergence, Artisanal gold mining, Animals, Humans, Parasitology, Gold, Passive surveillance
الوصف: PURPOSE: The boom in Burkina Faso's artisanal gold mining since 2007 has attracted populations from Côte d'Ivoire and Guinea, which are the West African countries most affected by human African trypanosomiasis (HAT) and therefore increases its risk of re-emergence. Our aim was to update the HAT data in Burkina Faso in the risk of the re-emergence context with the advent of artisanal gold mining.METHODS: The study was carried out in the southwestern Burkina Faso where entomological surveys were conducted using biconical traps in March 2017. Follow by an active medical survey in April 2017, which was targeted the gold panners in 7 villages closer to artisanal gold sites, using CATT, mini-anion exchange centrifugation technique, trypanolysis test (TL) and ELISA test to measure human/tsetse contacts. The buffy coat technique and the TL were also applied in pigs to check their reservoir role of human trypanosomes.RESULTS: Our results have shown no case of HAT among 958 individuals tested and all the 50 pigs were also negative, but the level of antibodies against tsetse saliva evidenced by ELISA revealed low human/tsetse contact. Moreover, gold panners practise agriculture and breeding in an infected tsetse area, which are increased the risk.CONCLUSION: Our results illustrate that the risk of re-emergence is low. The passive surveillance system implemented in 2015 in southwestern Burkina Faso is needed to increase the sentinel sites to better cover this area by taking into account the gold mining. Finally, awareness-raising activities are needed among populations about HAT.
وصف الملف: application/pdf
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9ead21ec0b8f8eb50d38b4a78ac712b
https://doi.org/10.1007/s11686-021-00512-2 -
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المؤلفون: Prisca S. L. Paré, Domonbabele F. D. S. Hien, Koama Bayili, Rakiswendé S. Yerbanga, Anna Cohuet, David Carrasco, Edwige Guissou, Louis-Clément Gouagna, Koudraogo B. Yaméogo, Abdoulaye Diabaté, Rickard Ignell, Roch K. Dabiré, Thierry Lefèvre, Olivier Gnankiné
المصدر: Scientific reports. 12(1)
مصطلحات موضوعية: Insecticide Resistance, Insecticides, Multidisciplinary, Mosquito Control, Anopheles, Pyrethrins, Humans, Animals, Mosquito Vectors, Diet
الوصف: Success in reducing malaria transmission through vector control is threatened by insecticide resistance in mosquitoes. Although the proximal molecular mechanisms and genetic determinants involved are now relatively well documented, little is known about the influence of the environment on the phenotypic expression of mosquito resistance to insecticides. In particular, the extent to which mosquito plant feeding affects the phenotypic expression of insecticide resistance remains unknown. To assess the effect of plant diet on the response of mosquitoes to insecticides, larvae from the predominant vector, Anopheles gambiae sensu latowere collected in southwestern Burkina Faso and reared until adulthood. Following emergence, adults were fed with one of four treatment (5% glucose solution, nectariferous flowers of Barleria lupulina, nectariferous flowers of Cascabela thevetia and a combination of both plants Barleria lupulina + Cascabela thevetia). WHO standard tube tests were performed with 0.05% and 0.5% deltamethrin. The measured end-point parameters were knockdown rate (KD) and the 24 h mosquito mortality. Following the bioassays, DNA was extracted from each mosquito individual for PCR identification of the siblings of the An. gambiae complex and determination of the kdr genotypic and allelic frequencies. Plant diet significantly influenced mosquito KD rate at both concentrations of deltamethrin. Following exposure to 0.05% deltamethrin, the Barleria lupulina diet induced a 2.5 fold-decrease in mosquito mortality compared to the 5% glucose treatment. The molecular data confirmed the predominance of An. gambiae (60% of the samples) over An. coluzzii and An. arabiensis. The kdr mutation L1014F displayed a high allelic frequency of 0.75 and, as expected, it was positively associated with increased resistance to deltamethrin. Plant diet, particularly B. lupulina, appears to increase the susceptibility of mosquitoes to insecticides. The finding that B. lupulina-fed control individuals (i.e., not exposed to deltamethrin)also displayed increased 24 h mortality suggests that plant-mediated effects may be driven by direct effect of plant diet on mosquito survival rather than indirect effects through interference with insecticide-resistance mechanisms. Thus, some plant species may weaken mosquitoes, making them less vigorous and more vulnerable to the insecticide. There is a need for further investigation, using a wider range of plant species and insecticides, in combination with other relevant environmental factors, to better understand the expression and evolution of insecticide resistance
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المؤلفون: Roch K. Dabiré, Maite Guardiola-Claramonte, Christophe Boëte, Claude C Habamungu, Severin N’do, Koama Bayili, R. Serge Yerbanga, Mahamadi Kientega, Didier A P Kaboré, Dieudonné Diloma Soma, Abdoulaye Diabaté, Bertin Z. Musaka, Adrien Marie Gaston Belem, Janvier B. Bandibabone, Moussa Namountougou, Jean-Bosco Ouédraogo, Ibrahim Sangaré, Jorian Prudhomme, Bantuzeko Chimanuka
المصدر: Transactions of The Royal Society of Tropical Medicine and Hygiene. 115:1339-1344
مصطلحات موضوعية: 0301 basic medicine, Insecticides, Veterinary medicine, Mosquito Control, Anopheles gambiae, 030231 tropical medicine, Mosquito Vectors, Fenitrothion, Insecticide Resistance, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Anopheles, Pyrethrins, parasitic diseases, medicine, Animals, Humans, biology, Public Health, Environmental and Occupational Health, General Medicine, Propoxur, biology.organism_classification, medicine.disease, Malaria, 030104 developmental biology, Infectious Diseases, Deltamethrin, chemistry, Vector (epidemiology), Democratic Republic of the Congo, Malathion, Parasitology
الوصف: Background Insecticide resistance has become a widespread problem causing a decline in the effectiveness of vector control tools in sub-Saharan Africa. In this situation, ongoing monitoring of vector susceptibility to insecticides is encouraged by the WHO to guide national malaria control programmes. Our study was conducted from April to November 2018 in Tchonka (Sud-Kivu, Democratic Republic of the Congo) and reported primary data on the resistance status of Anopheles funestus and Anopheles gambiae. Methods Insecticide susceptibility bioassays were performed on wild populations of A. funestus and A. gambiae using WHO insecticide-impregnated papers at discriminating concentration. In addition, PCR was performed to identify mosquito species and to detect kdr and ace-1R mutations involved in insecticide resistance. Results Bioassay results show resistance to all tested insecticides except pirimiphos-methyl, propoxur, fenitrothion and malathion with a mortality rate ranging from 95.48 to 99.86%. The addition of piperonyl butoxide (PBO) increased the susceptibility of vectors to deltamethrin and alpha-cypermethrin by exhibiting a mortality ranging from 91.50 to 95.86%. The kdr mutation was detected at high frequencies (approximately 0.98) within A. gambiae while ace-1R was not detected. Conclusions This study provides useful data on the insecticide resistance profiles of malaria vector populations to better manage vector control. Our results highlight that, despite the high level of resistance, organophosphorus compounds and pyrethroids + PBO remain effective against the vectors.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9396e10da1dbfda0d2cf1addb9e844af
https://doi.org/10.1093/trstmh/trab116 -
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المؤلفون: Simon P. Sawadogo, Didier A. Kabore, Ezechiel B. Tibiri, Angela Hughes, Olivier Gnankine, Shannon Quek, Abdoulaye Diabaté, Hilary Ranson, Grant L. Hughes, Roch K. Dabiré
مصطلحات موضوعية: General Veterinary, Mosquito Vectors, qx_45, Malaria, qx_20, Insect Science, RNA, Ribosomal, 16S, Anopheles, Burkina Faso, Animals, Parasitology, qx_515, Ecology, Evolution, Behavior and Systematics, Phylogeny, Wolbachia
الوصف: The endosymbiont Wolbachia can have major effects on the reproductive fitness, and vectorial capacity of host insects and may provide new avenues to control mosquito-borne pathogens. Anopheles gambiae s.l is the major vector of malaria in Africa but the use of Wolbachia in this species has been limited by challenges in establishing stable transinfected lines and uncertainty around native infections. High frequencies of infection of Wolbachia have been previously reported in An. gambiae collected from the Valle du Kou region of Burkina Faso in 2011 and 2014. Here, we re-evaluated the occurrence of Wolbachia in natural samples, collected from Valle du Kou over a 12-year time span, and in addition, expanded sampling to other sites in Burkina Faso. Our results showed that, in contrast to earlier reports, Wolbachia is present at an extremely low prevalence in natural population of An. gambiae. From 5341 samples analysed, only 29 were positive for Wolbachia by nested PCR representing 0.54% of prevalence. No positive samples were found with regular PCR. Phylogenetic analysis of 16S rRNA gene amplicons clustered across supergroup B, with some having similarity to sequences previously found in Anopheles from Burkina Faso. However, we cannot discount the possibility that the amplicon positive samples we detected were due to environmental contamination or were false positives. Regardless, the lack of a prominent native infection in An. gambiae s.l. is encouraging for applications utilizing Wolbachia transinfected mosquitoes for malaria control.
وصف الملف: application/pdf
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المؤلفون: Yawo Apetogbo, Koffi M. Ahadji-Dabla, Dieudonné Diloma Soma, Adjovi D. Amoudji, Edoh Koffi, Kossivi I. Akagankou, Rabila Bamogo, Kelly Lionelle Ngaffo, Samina Maiga, Rachid T. Atcha‑Oubou, Ameyo M. Dorkenoo, Lucrecia Vizcaino, Audrey Lenhart, Abdoulaye Diabaté, Roch Kounbobr Dabiré, Guillaume Koffivi Ketoh
المصدر: Malaria journal. 21(1)
مصطلحات موضوعية: Insecticide Resistance, Insecticides, Infectious Diseases, Togo, Anopheles, Pyrethrins, Animals, Parasitology
الوصف: Background This study was designed to provide insecticide resistance data for decision-making in terms of resistance management plans in Togo. Methods The susceptibility status of Anopheles gambiae sensu lato (s.l.) to insecticides used in public health was assessed using the WHO tube test protocol. Pyrethroid resistance intensity bioassays were performed following the CDC bottle test protocol. The activity of detoxification enzymes was tested using the synergists piperonyl butoxide, S.S.S-tributlyphosphorotrithioate and ethacrinic acid. Species-specific identification of An. gambiae s.l. and kdr mutation genotyping were performed using PCR techniques. Results Local populations of An. gambiae s.l. showed full susceptibility to pirimiphos methyl at Lomé, Kovié, Anié, and Kpèlè Toutou. At Baguida, mortality was 90%, indicating possible resistance to pirimiphos methyl. Resistance was recorded to DDT, bendiocarb, and propoxur at all sites. A high intensity of pyrethroid resistance was recorded and the detoxification enzymes contributing to resistance were oxidases, esterases, and glutathione-s-transferases based on the synergist tests. Anopheles gambiae sensu stricto (s.s.) and Anopheles coluzzii were the main species identified. High kdr L1014F and low kdr L1014S allele frequencies were detected at all localities. Conclusion This study suggests the need to reinforce current insecticide-based malaria control interventions (IRS and LLINs) with complementary tools.
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المؤلفون: Yérobessor Dabiré, Namwin Siourimè Somda, Marius K. Somda, Clarisse B. Compaoré, Iliassou Mogmenga, Lewis I. Ezeogu, Alfred S. Traoré, Jerry O. Ugwuanyi, Mamoudou H. Dicko
المصدر: BMC microbiology. 22(1)
مصطلحات موضوعية: Microbiology (medical), Prostaglandins A, Phenol, Probiotics, Glutamic Acid, Bacillus, Lipase, Microbiology, Neoptera, Anti-Bacterial Agents, Bile Acids and Salts, Anti-Infective Agents, Amylases, Endopeptidases, Animals, Humans, Fermented Foods, Peptide Hydrolases
الوصف: Background Soumbala is a highly loved alkaline traditional fermented food condiment in Burkina Faso. It harbors various microbiota dominated by fermentative Bacillus spp. as functional microorganism with little confirmed health-promoting properties. Methods The present study aimed to evaluate six Bacillus strains previously isolated and identified from soumbala. These strains were selected as presumptively safe bacteria for probiotic and technological characteristics. These strains were assessed for in vitro probiotic criteria (tolerance to acidic pH, gastric juice, 0.3% (m/v) bile salts, intestinal juice and 0.4% (w/v) phenol, cell surface hydrophobicity, auto-aggregation capacity, antimicrobial activity against foodborne pathogens, antibiotic susceptibility and biofilm production) and technological properties, including protease, amylase, lipase, and tannase activity, as well as poly-γ-glutamic acid (PGA) production and thermo-tolerance. Results All tested Bacillus strains (B54, F20, F24, F21, F26 and F44) presented variable relevant probiotic properties (good tolerance to pH 2 and pH 4, gastric juice, bile salts, intestinal juice and phenol), with marked differences in hydrophobicity and auto-aggregation capacity ranging from 73.62—94.71% and 49.35—92.30%, respectively. They exhibited a broad spectrum of activity against foodborne pathogens depending on target pathogen, with the highest activity exhibited by strain F20 (29.52 mm) against B. cereus 39 (p < 0.001). They also showed good biofilm production as well as variable hydrolytic enzyme activities, including protease (43.00—60.67 mm), amylase (22.59—49.55 mm), lipase (20.02—24.57 mm), and tannase (0—10.67 mm). All tested Bacillus strains tolerated temperature up to 50 °C, while only strains F26 and F44 showed the best PGA production. Conclusion Overall, the tested cultures exhibiting potential probiotic and technological characteristics; particularly B. cereus F20, B. benzoevorans F21, B. cabrialessi F26, and B. tequilensis F44 could be a source of probiotic-starters of commercial interest in the production of high-quality soumbala.
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