المؤلفون: Jankowska K; University of Warsaw, Faculty of Biology, Biological and Chemical Research Centre, ul. Żwirki i Wigury 101, 02-089 Warsaw, Poland., Łukomska-Kowalczyk M; University of Warsaw, Faculty of Biology, Biological and Chemical Research Centre, ul. Żwirki i Wigury 101, 02-089 Warsaw, Poland. Electronic address: m.lukomskako@uw.edu.pl., Milanowski R; University of Warsaw, Faculty of Biology, Biological and Chemical Research Centre, ul. Żwirki i Wigury 101, 02-089 Warsaw, Poland., Fells A; University of Warsaw, Faculty of Biology, Biological and Chemical Research Centre, ul. Żwirki i Wigury 101, 02-089 Warsaw, Poland., Zakryś B; University of Warsaw, Faculty of Biology, Biological and Chemical Research Centre, ul. Żwirki i Wigury 101, 02-089 Warsaw, Poland.

المصدر: Protist [Protist] 2024 Jun; Vol. 175 (3), pp. 126024. Date of Electronic Publication: 2024 Mar 04.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: G. Fischer Country of Publication: Germany NLM ID: 9806488 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0941 (Electronic) Linking ISSN: 14344610 NLM ISO Abbreviation: Protist Subsets: MEDLINE

مواضيع طبية MeSH: DNA Barcoding, Taxonomic* , Biodiversity* , Euglenida*/genetics , Euglenida*/classification, Poland ; RNA, Ribosomal, 18S/genetics ; Phylogeny ; DNA, Protozoan/genetics ; Autotrophic Processes ; DNA, Ribosomal/genetics

مستخلص: This study reports a comprehensive analysis of photoautotrophic euglenids' distribution and biodiversity in 16 small water bodies of various types (including fish ponds, field ponds, rural ponds and park ponds) located in three regions of Poland: Masovia, Masuria and Pomerania during a period of three years. By employing a euglenid specific barcode marker and a curated database of V2 18S rDNA sequences it was possible to identify 97.7 % of euglenid reads at species level. A total of 152 species classified in 13 genera were identified. The number of euglenid species found in one pond varied from 40 to 102. The most common species were Euglena agilis and Euglenaria caudata, found in every analysed waterbody. The highest number of observed species belonged to Trachelomonas and Phacus. Certain species exhibited a tendency to coexist, suggesting the presence of distinct species assemblages. Among them, the most distinctive cluster was associated with water bodies located in the Masuria region, characterized also by the greatest species richness, including many very rare species: Euglenaformis chlorophoenicea, Lepocinclis autumnalis, L. marssonii, Trachelomonas eurystoma, T. manschurica, T. mucosa, T. zuberi, T. zuberi var. nepos.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier GmbH.. All rights reserved.)

المؤلفون: Li, Jiamin^{Aff1, Aff2, Aff3, Aff4}, Liu, Feng^{Aff2, Aff3, Aff4, IDs10811024031852_cor2}, Yan, Tian^{Aff2, Aff3, Aff4}, Chen, Nansheng^{Aff2, Aff3, Aff4}, Liu, Liang

المصدر: Journal of Applied Phycology. 36(3):1363-1376

المؤلفون: James Omondi Outa, Annemariè Avenant-Oldewage

المصدر: Parasitology, Vol 151, Pp 108-124 (2024)

مصطلحات موضوعية: biodiversity, blood flukes, brevifurcate cercaria, Burnupia, Crocodile River, lophocercous-apharyngeate, rDNA sequences, scanning electron microscopy, Vaal River, Biochemistry, QD415-436, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502

الوصف: Blood flukes of freshwater fish are understudied worldwide. Consequently, genetic information and data on their intramolluscan stages are scarce. In the current study, freshwater limpets of the genus Burnupia (Burnupiidae) from South Africa were examined for digeneans. Of 1645 specimens, 3.10% were infected by Sanguinicolidae larvae. Four sanguinicolids were distinguished by body size, number of penetration glands, tegumental spines’ patterns and relative sizes of the finfolds on the body and furcae. Analyses of 28S, 18S and ITS rDNA sequences showed that the morphotypes were distinct from each other and from sanguinicolids whose genetic data are available. The present study is the first genetic characterization of sanguinicolids from Africa. Phylogenetic reconstruction revealed that the present species clustered with a sanguinicolid from Poland and were sister to Sanguinicola and Pseudosanguinicola from Russia and USA, respectively. The results indicate that the current species represent an unknown genus. What is more, blood fluke sequences from East Africa (presumed to be sanguinicolids), were distant from Sanguinicolidae and showed a closer relationship with acipensericolids from the USA. Freshwater fish blood flukes seem to be more diverse than previously recorded and use species of at least 13 gastropod families as intermediate hosts.

وصف الملف: electronic resource

Relation: https://www.cambridge.org/core/product/identifier/S003118202300121X/type/journal_article; https://doaj.org/toc/0031-1820; https://doaj.org/toc/1469-8161

URL الوصول: https://doaj.org/article/b40e507f8fbf4e55873078b23118c6f6

المؤلفون: Ritter CD; a Department of Eukaryotic Microbiology, University of Duisburg-Essen, Universitätsstrasse 5 S05 R04 H83 D-45141 Essen, Germany., Häggqvist S; b Swedish Museum of Natural History, Department of Zoology, Box 50007, 104 05 Stockholm, Sweden., Karlsson D; c Station Linné, Skogsby 161, 386 94 Färjestaden, Sweden., Sääksjärvi IE; d Biodiversity Unit, University of Turku, FI-20014 Turku, Finland., Muasya AM; e Department of Biological Sciences, University of Cape Town, Rondebosch 7701, RSA., Nilsson RH; f Gothenburg Global Biodiversity Centre, Box 461, SE-405 30 Göteborg, Sweden.; g Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, 405 30 Göteborg, Sweden., Antonelli A; f Gothenburg Global Biodiversity Centre, Box 461, SE-405 30 Göteborg, Sweden.; g Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, 405 30 Göteborg, Sweden.; h Gothenburg Botanical Garden, Carl Skottsbergs gata 22A, SE-41319 Göteborg, Sweden.

المصدر: Genome [Genome] 2019 Mar; Vol. 62 (3), pp. 147-159. Date of Electronic Publication: 2019 Jan 23.

نوع المنشور: Historical Article; Journal Article

بيانات الدورية: Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 8704544 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1480-3321 (Electronic) Linking ISSN: 08312796 NLM ISO Abbreviation: Genome

مواضيع طبية MeSH: Biodiversity*, DNA/*genetics , DNA Barcoding, Taxonomic/*methods , Eukaryota/*genetics , Insecta/*classification , Insecta/*genetics , Prokaryotic Cells/*metabolism, Animals ; Brazil ; DNA/analysis ; Ecosystem ; High-Throughput Nucleotide Sequencing ; History, 21st Century ; South Africa

مستخلص: The rapid loss of biodiversity, coupled with difficulties in species identification, call for innovative approaches to assess biodiversity. Insects make up a substantial proportion of extant diversity and play fundamental roles in any given ecosystem. To complement morphological species identification, new techniques such as metabarcoding make it possible to quantify insect diversity and insect-ecosystem interactions through DNA sequencing. Here we examine the potential of bulk insect samples (i.e., containing many non-sorted specimens) to assess prokaryote and eukaryote biodiversity and to complement the taxonomic coverage of soil samples. We sampled 25 sites on three continents and in various ecosystems, collecting insects with SLAM traps (Brazil) and Malaise traps (South Africa and Sweden). We then compared our diversity estimates with the results obtained with biodiversity data from soil samples from the same localities. We found a largely different taxonomic composition between the soil and insect samples, testifying to the potential of bulk insect samples to complement soil samples. Finally, we found that non-destructive DNA extraction protocols, which preserve insect specimens for morphological studies, constitute a promising choice for cost-effective biodiversity assessments. We propose that the sampling and sequencing of insect samples should become a standard complement for biodiversity studies based on environmental DNA.

المؤلفون: Liu, X. J., Wen, W. J., Yang, X. X., Hu, H. Y., Ji, T. Ch., Liu, H. M., Zhu, G. P.^{Aff1, IDS1995082922040447_cor7}

المصدر: Inland Water Biology. 15(4):403-409

المؤلفون: Cezar RM; Post-Graduate in Soil Science, Federal University of Paraná, Rua Dos Funcionários, 1540, Curitiba, PR, CEP 80035-050, Brazil., Vezzani FM; Post-Graduate in Soil Science, Federal University of Paraná, Rua Dos Funcionários, 1540, Curitiba, PR, CEP 80035-050, Brazil., Kaschuk G; Post-Graduate in Soil Science, Federal University of Paraná, Rua Dos Funcionários, 1540, Curitiba, PR, CEP 80035-050, Brazil. glaciela.kaschuk@ufpr.br., Balsanelli E; Department of Biochemistry, Federal University of Paraná, Rua Francisco H. Dos Santos S/N, Curitiba, PR, CEP 81531-990, Brazil., de Souza EM; Department of Biochemistry, Federal University of Paraná, Rua Francisco H. Dos Santos S/N, Curitiba, PR, CEP 81531-990, Brazil., Vargas LK; Department of Agricultural Diagnosis and Research, Secretary of Agriculture and Livestock of the State of Rio Grande Do Sul, Rua Gonçalves Dias, 570, Porto Alegre, RS, CEP 90130-060, Brazil., Molin R; ABC Foundation, Rod. PR 151 km 288, Caixa-postal: 1003, Castro, PR, CEP 84165-700, Brazil.

المصدر: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2021 Dec; Vol. 52 (4), pp. 2169-2177. Date of Electronic Publication: 2021 Jul 28.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE

مواضيع طبية MeSH: Agriculture*/methods , Bacteria, Anaerobic*/physiology , Biodiversity* , Soil Microbiology*, Bacteria/genetics ; Brazil ; Crop Production ; Oxygen ; RNA, Ribosomal, 16S/genetics

مستخلص: Crop diversity affects the processes of soil physical structuring and most likely provokes changes in the frequencies of soil microbial communities. The study was conducted for soil prokaryotic diversity sequencing 16S rDNA genes from a 25-year no-tillage experiment comprised of two crop systems: crop succession (Triticum aestivum-Glycine max) and rotation (Vicia sativa-Zea mays-Avena sativa-Glycine max-Triticum aestivum-Glycine max). The hypothesis was that a crop system with higher crop diversification (rotation) would affect the frequencies of prokaryotic taxa against a less diverse crop system (succession) altering the major soil functions guided by bacterial diversity. Soils in both crop systems were dominated by Proteobacteria (31%), Acidobacteria (23%), Actinobacteria (10%), and Gemmatimonadetes (7.2%), among other common copiotrophic soil bacteria. Crop systems did not affect the richness and diversity indexes of soil bacteria and soil archaea. However, the crop rotation system reduced only the frequencies of anaerobic metabolism bacteria Chloroacidobacteria, Holophagae, Spirochaetes, Euryarchaeota, and Crenarchaeota. It can be concluded that crop succession, a system that is poorer in root diversity over time, may have conditioned the soil to lower oxygen diffusion and built up ecological niches that suitable for anaerobic bacteria tolerating lower levels of oxygen. On the other hand, it appeared that crop rotation has restructured the soil over the years while enabling copiotrophic aerobic bacteria to dominate the soil ecosystem. The changes prompted by crop succession have implications for efficient soil organic matter decomposition, reduced greenhouse gas emissions, higher root activity, and overall soil productivity, which compromise to agriculture sustainability.
(© 2021. Sociedade Brasileira de Microbiologia.)

المؤلفون: Yong Zhang, Ying Yu, Zhishuai Qu, Mengmeng Jiang, Zhuo Shen, Jiqiu Li, Xiaofeng Lin

المصدر: Frontiers in Marine Science, Vol 9 (2022)

مصطلحات موضوعية: biodiversity, morphology, molecular phylogeny, sessile ciliates, SSU rDNA, Science, General. Including nature conservation, geographical distribution, QH1-199.5

الوصف: Peritrich ciliates are a species-rich group of sessile unicellular eukaryotes, which can be found in various aquatic habitats from all over the world. It is well accepted that there are still many ciliates to be uncovered. During a survey on ciliate biodiversity in the coastal waters of China, three solitary peritrich species were identified as members of the genus Pseudovorticella Foissner & Schiffmann, 1975, including two new species and a rare one. Pseudovorticella zhejiangensis sp. n. differs from its congeners mainly by having a conical-shaped zooid, conspicuous pellicular blisters, one ventral and one dorsal contractile vacuoles, and an infundibular polykinety 3 with three rows of nearly equal length but different beginning positions. Pseudovorticella dalianensis sp. n. can be defined mainly by an obovoid-shaped zooid, one ventral contractile vacuole, and a three-rowed infundibular polykinety 3 with the middle row being the longest. The rare species, Pseudovorticella verrucosa (Dons, 1915) Sun et al., 2009, was redescribed. The small subunit (SSU) rDNA sequences of these three species were sequenced for the first time, the phylogeny of Pseudovorticella species was analyzed, and the results verified the non-monophyly of this genus. This study demonstrates that the morphologic and gene barcoding data are the optimum combination to disclose the biodiversity of ciliates.

وصف الملف: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fmars.2022.1030519/full; https://doaj.org/toc/2296-7745

URL الوصول: https://doaj.org/article/79ff6ceafa574ad7862be68b046e825d

المؤلفون: Picon A; INIA, Departamento de Tecnología de Alimentos, Carretera de La Coruña Km 7, 28040, Madrid, Spain. Electronic address: apicon@inia.es., Del Olmo A; INIA, Departamento de Tecnología de Alimentos, Carretera de La Coruña Km 7, 28040, Madrid, Spain., Nuñez M; INIA, Departamento de Tecnología de Alimentos, Carretera de La Coruña Km 7, 28040, Madrid, Spain.

المصدر: Food microbiology [Food Microbiol] 2021 Apr; Vol. 94, pp. 103646. Date of Electronic Publication: 2020 Sep 24.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 8601127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9998 (Electronic) Linking ISSN: 07400020 NLM ISO Abbreviation: Food Microbiol Subsets: MEDLINE

مواضيع طبية MeSH: Biodiversity*, Bacteria/*isolation & purification , Chlorophyta/*microbiology , Seaweed/*microbiology, Bacteria/chemistry ; Bacteria/classification ; Bacteria/genetics ; Food Handling/instrumentation ; Food Handling/methods ; Food Handling/statistics & numerical data ; Food Storage ; Vegetables/microbiology

مستخلص: Seaweeds are highly perishable foods due to their richness in nutrients. High pressure processing (HPP) has been applied for extending the shelf life of fresh seaweeds but there is no information on the effect of HPP on the bacterial diversity of seaweeds. The culturable bacteria of six species of fresh edible seaweeds (green seaweeds Codium fragile and Ulva lactuca, brown seaweeds Himanthalia elongata, Laminaria ochroleuca and Undaria pinnatifida, and red seaweed Chondrus crispus) were investigated and compared to those of HPP-treated (400 and 600 MPa for 5 min) seaweeds, at the start and end of their refrigerated storage period. A total of 523 and 506 bacterial isolates were respectively retrieved from untreated and HPP-treated seaweeds. Isolates from untreated seaweeds belonged to 18 orders, 35 families, 71 genera and 135 species whereas isolates from HPP-treated seaweeds belonged to 13 orders, 23 families, 43 genera and 103 species. HPP treatment significantly reduced the number of isolates belonging to 6 families and greatly increased the number of Bacillaceae isolates. At the end of storage, decreases in bacterial diversity at the genus and species level were observed for untreated as well as for HPP-treated seaweeds.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)

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المؤلفون: Rui Wang, Yang Bai, Tao Hu, Dapeng Xu, Toshikazu Suzuki, Xiaozhong Hu

المصدر: BMC Ecology and Evolution, Vol 21, Iss 1, Pp 1-23 (2021)

مصطلحات موضوعية: Biodiversity, loricate ciliates, new combination, new genus, rDNA, Tintinnina, Ecology, QH540-549.5, Evolution, QH359-425

الوصف: Abstract Background The taxonomic classification of the suborder Tintinnina Kofoid & Campbell, 1929, a species-rich group of planktonic ciliated protistans with a characteristic lorica, has long been ambiguous largely due to the lack of cytological and molecular data for most species. Tintinnopsis is the largest, most widespread, and most taxonomically complex genus within this group with about 170 species occurring in nearshore waters. Previous molecular phylogenetic studies have revealed that Tintinnopsis is polyphyletic. Results Here we document the live morphology, infraciliature, gene sequences, and habitat characteristics of three poorly known tintinnine species, viz. Tintinnopsis karajacensis Brandt, 1896, Tintinnopsis gracilis Kofoid & Campbell, 1929, and Tintinnopsis tocantinensis Kofoid & Campbell, 1929, isolated from the coastal waters of China. Based on a unique cytological feature (i.e., an elongated ciliary tuft with densely arranged kinetids) in the former two species, Antetintinnopsis gen. nov. is erected with Antetintinnopsis hemispiralis (Yin, 1956) comb. nov. (original combination: Tintinnopsis hemispiralis Yin, 1956) designated as the type species. Moreover, A. karajacensis (Brandt, 1896) comb. nov. (original combination: Tintinnopsis karajacensis Brandt, 1896) and A. gracilis (Kofoid & Campbell, 1929) comb. nov. (original combination: Tintinnopsis gracilis Kofoid & Campbell, 1929) are placed in a highly supported clade that branches separately from Tintinnopsis clades in phylogenetic trees based on SSU rDNA and LSU rDNA sequence data, thus supporting the establishment of the new genus. One other species is assigned to Antetintinnopsis gen. nov., namely A. subacuta (Jörgensen, 1899) comb. nov. (original combination Tintinnopsis subacuta Jörgensen, 1899). Conclusions The findings of the phylogenetic analyses support the assertion that cytological characters are taxonomically informative for tintinnines. This study also contributes to the broadening of our understanding of the tintinnine biodiversity and evolution.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2730-7182

URL الوصول: https://doaj.org/article/3ffc7247cb8a414a8d7888a8546a4dce