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المؤلفون: N. T. Lien, R. V. Doudkin, Q. V. Hoi, B. B. Thinh, L. D. Chac, H. V. Chinh
المصدر: Труды по прикладной ботанике, генетике и селекции, Vol 182, Iss 2, Pp 114-122 (2021)
مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, sequence analysis, Physiology, Sequence analysis, molecular identification, Plant Science, Computational biology, Biology, Mega, 01 natural sciences, Biochemistry, DNA barcoding, DNA sequencing, law.invention, matk, 03 medical and health sciences, chemistry.chemical_compound, law, Genetics, its, rpoc, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Polymerase chain reaction, Phylogenetic tree, menispermaceae, rbcl, Botany, 030104 developmental biology, chemistry, QK1-989, Taxonomy (biology), TP248.13-248.65, DNA, 010606 plant biology & botany, Biotechnology
الوصف: Background. Tinomiscium petiolare Hook.f. & Thomson is a medicinal species of the family Menispermaceae. This species is currently being intensively exploited for therapeutic purposes. Precise and rapid identification of T. petiolare is critical and essential for the classification, propagation, use and conservation of its genetic resources. In recent years, DNA barcoding has been known to be a fast and sensitive method for identifying species at any stage of development, using short DNA sequences. In this study we have performed the identification of T. petiolare specimens in Vietnam based on the sequence analysis of 4 DNA barcode loci: ITS, matK, rbcL and rpoC.Materials and methods. Total DNA was extracted from leaf samples using DNeasy Plant Mini Kit. PCR amplification of the ITS, matK, rbcL and rpoC regions was carried out on the GeneAmp PCR System 9700 with specific primers. The purified PCR products were sequenced on the ABI 3500 Genetic Analyzer system, using BigDye®Terminator v3.1 Cycle Sequencing Kit. These genetic sequences were analyzed and compared, and a phylogenetic tree was constructed using BioEdit, BLAST, and MEGA 6 programs.Results and conclusion. The success rate of amplification and sequencing was 100% for all 4 DNA barcode loci (ITS, matK, rbcL and rpoC) in the studied specimens. The produced sequence sizes of ITS, matK, rbcL and rpoC in the specimens were 574 bp, 810 bp, 527 bp and 488 bp, respectively. Further, we identified that all studied specimens were genetically related to each other and associated with the same species T. petiolare. Overall, the results of the study generated the most complete DNA barcode database of T. petiolare collected in Vietnam, contributing to the taxonomy and identification of this species.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16cbad4d6d95ca32753d3167784ea07a
https://doi.org/10.30901/2227-8834-2021-2-114-122 -
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المؤلفون: Tine Grebenc, Hojka Kraigher, Tina Unuk Nahberger
المصدر: Folia biologica et geologica, str. 229-238, Letn. 61, št. 2 : Ilustr., 2020
COBISS-ID: 248490496مصطلحات موضوعية: Base pair, Biology, Tuber spp., ITS regija, PCR pomnoževanje, ITS začetni oligonukleotidi, ektomikorizne glive, glivne združbe, biology.organism_classification, Molecular biology, DNA sequencing, law.invention, chemistry.chemical_compound, chemistry, law, Genetic marker, Tuber aestivum, Primer (molecular biology), udc:630*1, Genus Tuber, Tuber spp., ITS region, PCR amplification, ITS primers, Polymerase chain reaction, DNA
الوصف: Since late 20th century DNA sequencing became the method of choice method in precision species identification. The ITS region is one of the official fungal barcoding DNA markers, although in some cases sequencing of the ITS region may, due to misidentification, mislabeling or nomenclature errors in public databases, lead to incorrect or insufficient identification, as is currently a case in the genus Tuber. The aim of this study was to test, which ITS primer pairs are most appropriate and optimal for Tuber species DNA region amplification. Thereby we (1) compared amplification success for different Tuber species using fungal specific primer pair ITS1f and ITS4 and (2) compared amplification success using different ITS primer pair combinations in amplifying DNA region an example species Tuber aestivum. Based on results, Tuber aestivum was one of the most reluctant Tuber species in this study and in most cases failed to amplify with the above primer pair. After comparing different ITS primer pairs, we conclude that the primer pair ITS5 and ITS7 is the most appropriate primer pair for amplification DNA region of T. aestivum as it resulted in high amplification success from ectomycorrhizal root tips. Based on sequences, gained from public databases, we found that ITS1f and ITS6 primers have a mismatch in one base pair compared to the target sequence of Tuber aestivum, thus resulting in poor or no amplification success. Although primer pair ITS5 and ITS7 in our study was proven to be the most appropriate primer pair in amplifying DNA region Tuber aestivum species, further analysis about appropriateness of it for a general barcoding and identification of ectomycorrhiza in complex community samples is needed. Keywords: Tuber spp., ITS region, PCR amplification, ITS primers Izvleček Od konca 20. stoletja je določanje nukleotidnega zaporedja DNA postalo ena izmed pogosteje uporabljenih metod za določanje vrst. ITS regija je edna izmed uradnih glivnih DNA markerjev, čeprav lahko določanje nukleotidnega zaporedja le-te, v nekaterih primerih, predvsem zaradi napačne določitve, označevanja oziroma napak v nomenklaturi v javnih bazah podatkov, privede do napačne oziroma nenatančne določitve vrst, kar je trenutno težava pri določitvi vrst iz rodu Tuber. Namen te študije je bil testirati kateri pari ITS začetnih oligonukleotidov so najbolj primerni in optimalni za pomnoževanje DNA regij gliv iz rodu Tuber. S tem namenom smo v študiji (1) primerjali uspešnost pomnoževanja DNA regije različnih vrst iz rodu Tuber, z uporabo glivno specifičnih začetnih oligonukleotidov ITS1f in ITS4 ter hkrati (2) primerjali uspešnost pomnoževanja DNA regije vrste Tuber aestivum z uporabo različnih ITS začetnih oligonukleotidov. Na podlagi rezultatov ugotavljamo, da je vrsta T. aestivum izmed vseh analiziranih gliv iz rodu Tuber, bila najtežavnejša vrsta v naši študiji, saj je v večini primerov pomnoževanje DNA regije te vrste z uporabo glivno specifičnih začetnih oligonukleotidov ITS1f in ITS4 bilo neuspešno. Po primerjavi uspešnosti pomnoževanja z različnimi ITS začetnimi oligonukelotidi ugotavljamo, da sta bila v naši študiji ITS začetna oligonukleotida ITS5 in ITS7 najprimernejša za pomnoževanje DNA regije vrste T. aestivum, saj je bila uspešnost pomnoževanja iz ektomikoriznih vršičkov v tem primeru največja. Na podlagi T. aestivum nukleotidnih zaporedij pridobljenih iz javnih podatkovnih baz ugotavljamo, da je za začetna oligonukleotida ITS1f in ITS6 značilno neujemanje s tarčnim nukleotidnim zaporedjem (T. aestivum) v enem baznem paru, kar se lahko odraža bodisi v slabšem pomnoževalnem uspehu ali v nepomnoževanju na splošno. Kljub temu, da v naši študiji ugotavljamo, da sta začetna oligonukleotida ITS5 in ITS7 najprimernejša za pomnoževanje DNA regije glive T. aestivum, so potrebne nadaljnje analize, s katerimi bi potrdili splošno primernost omenjenega para ITS5/ITS7 za pomnoževanje DNA regije ne samo vrst iz rodu Tuber, temveč za določanje ektomikoriznih glivnih združb na splošno. Ključne besede: Tuber spp., ITS regija, PCR pomnoževanje, ITS začetni oligonukleotidi
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc16d0096f6d320906d62876c8f016aa
https://doi.org/10.3986/fbg0076 -
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المؤلفون: İjlal Ocak, Safiye Elif Korcan, Semra Malkoç, Arzu Ünal
المصدر: Volume: 9, Issue: 3 1674-1682
Journal of the Institute of Science and Technologyمصطلحات موضوعية: Fungi,isolation,Internal Transcribed Spacer (ITS),amylase enzyme, Biology, Food Science and Technology, Molecular biology, Fungus,izolasyon,Internal Transcribed Spacer (ITS),amilaz enzimi, Gıda Bilimi ve Teknolojisi
الوصف: Bu çalışmada ekmek yapımında ticari önemi olan alfa-amilaz enzimi üreten termofilik ve mezofilik yerli fungusların izolasyonu, makroskobik, mikroskobik ve moleküler tür teşhisleri gerçekleştirilmiştir. Mikroorganizma Kültür Koleksiyon Merkezlerine ve Mikrobiyal Gen Bankalarına genetik materyal oluşturulması ve yerli izolatlardan patente konu yerli enzim üretimi amaçlanmıştır. Afyon, Eskişehir, Uşak, Ankara termal alanlardan 23 termotolerant fungus izole edilmiştir. İzolatların makroskobik ve mikroskobik incelemeleri sonucunda yüksek amilaz enzimi üreten Aspergillus niger, Aspergillus terreus ve Trichoderma atroviride türüne ait 6 izolatın tür teşhisi yapılmıştır. İzolatların fungus spesifik primerleri kullanılarak 18S rDNA ve ITS bölgeleri PCR amplifikasyonu, moleküler tanımlaması yapılmış ve Web tabanlı BLAST analizleri ile karşılaştırılmıştır. Moleküler karakterizasyon çalışması sonucunda klasik tanımlama ile moleküler tanımlamanın birbirini desteklediği görülmüştür.
In this study, isolation of thermophilic and mesophilic native fungi producing alpha-amylase enzyme which is commercially important in bread making, macroscopic, microscopic and molecular identification were performed. Microorganism Culture Collection Centers and Microbial GenBanks to create genetic material and local isolates to produce patents of the native enzyme was aimed. 23 thermotolerant fungi were isolated from the thermal areas of Afyon, Eskişehir, Uşak and Ankara. As a result of macroscopic and microscopic investigations of isolates, 6 isolates of Aspergillus niger, Aspergillus terreus and Trichoderma atroviride species which produce high amylase enzyme species were determined. 18S rDNA and ITS regions of the isolates were compared with Web-based BLAST analysis by PCR amplification and molecular identification. As a result of molecular characterization study, it was observed that the classical identification and the molecular identification supported each other.وصف الملف: application/pdf
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a828b3b6b9aa891c116224ec8ea5c43
https://doi.org/10.21597/jist.542215 -
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المؤلفون: Piotr Osyczka, Anna Lenart-Boroń, Piotr Boroń, Kaja Rola
المصدر: Mycologia. 113:43-55
مصطلحات موضوعية: Trebouxia, Genotype, Lichens, Physiology, disturbed habitat, Biology, lichenized fungi, DNA, Algal, Ascomycota, Algae, Chlorophyta, Metals, Heavy, DNA, Ribosomal Spacer, Genetics, Soil Pollutants, Hyperaccumulator, Symbiosis, Lichen, Molecular Biology, Phylogeny, Ecology, Evolution, Behavior and Systematics, Cladonia, Ecology, phylogenetic analysis, algal partner, Cell Biology, General Medicine, biology.organism_classification, stomatognathic diseases, Habitat, Species richness, ITS, Epigeal, Environmental Pollution, symbiotic adaptivity, mycobiont selectivity
الوصف: Mycobionts of many lichen genera appear to demonstrate strong selectivity in the choice of algal partner. The biological properties of a photobiont and its availability in an environment significantly determine the habitat requirements of lichens. Flexibility in photobiont choice extends the ecological amplitude of lichens; therefore, it may constitute an important adaptive strategy for colonization of extreme habitats. The photobiont inventory of the three epigeic lichens most resistant to soil pollution, i.e., Cladonia cariosa, C. rei, and the hyperaccumulator Diploschistes muscorum, was examined to verify whether and to what extent algal composition depends on the type of habitat and substrate enrichment with heavy metals. Photobionts Asterochloris and Trebouxia were identified in the studied lichen species; however, the presence of Trebouxia was directly related to anthropogenic sites with technogenic substrates, and the proportion of lichen specimens with these algae clearly depended on the level of heavy-metal soil pollution and the habitat type. The total number of algal haplotypes increased with increasing soil pollution, and the richness was associated more with soil pollution than with a given lichen species. Additionally, a large number of lichen individuals bearing multiple algal genotypes at polluted sites were recorded. Although Cladonia lichens were previously thought to be restricted to Asterochloris, they are able to start the relichenization process with Trebouxia under specific habitat conditions and to establish a stable association with these algae when colonization of disturbed sites takes place. Comparative analysis of the internal transcribed spacer (ITS) rDNA sequences revealed as many as 13 haplotypes of Trebouxia, and phylogenetic analysis grouped them into two different clades. Such a high level of genetic diversity indicates that Trebouxia is well adapted to metal pollution and could be an alternative photosynthetic partner for certain lichens, especially in polluted sites.
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المؤلفون: Emre Evlice, Ahmet Altaş, Göksel Özer, Mustafa Imren, Abdelfattah A. Dababat
المساهمون: BAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümü, Özer, Göksel, İmren, Mustafa
المصدر: Volume: 44, Issue: 3 385-397
Turkish Journal of Entomologyمصطلحات موضوعية: Morphology, İzmir, Fen, biology, Globodera rostochiensis, Science, Filogenetik, biology.organism_classification, Globodera rostochiensis,ITS,İzmir,morphology,phylogeny, Heteroderidae, Molecular biology, Tylenchida, Insect Science, Izmir, Globodera rostochiensis,ITS,İzmir,morfoloji,filogenetik, Globodera Rostochiensis, ITS, Morfoloji, Phylogeny
الوصف: The golden nematode, Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 (Tylenchida: Heteroderidae) is one of the most economically important pests of potato in the world. Tests for this nematode are routinely performed for outbreaks and densities in potato growing areas. The morphological and molecular analyses for precise determination of the nematode are employed to establish appropriate management strategies. This study showed that 96% of soil samples obtained from Bozdağ and Ödemiş Districts of İzmir Province, during 2017 and 2018 potato growing seasons, were positive for G. rostochiensis. The mean number of cysts ranged from 0.01 to 3.70 cysts g-1 soil in the fields examined. The examination of the morphological and morphometric features of the second-stage juveniles and cysts of the G. rostochiensis revealed slight differences among the populations obtained from Bozdağ and Ödemis. To assess the accuracy of the identification, partial sequences of ribosomal DNA for all populations were amplified, sequenced, and deposited in GenBank. The comparisons of the sequences with those of corresponding G. rostochiensis populations available in GenBank showed 99.89-100% nucleotide similarity. The results of this study will help to better understand the physiology, ecology and biology of the nematode to quarantine this pest more effectively.
Altın nematod, Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 (Tylenchida: Heteroderidae) dünyada ekonomik açıdan en önemli patates zararlılarından birisidir. Genellikle patates yetiştirilen alanlarındaki dağılımları ve yoğunluklarını belirlemek amacıyla bu nematod için rutin olarak testler gerçekleştirilmektedir. Morfolojik ve moleküler analizler bu nematodun kesin tanısını yapmak ve mücadele stratejilerini oluşturmak amacıyla kullanılmaktadır. Bu çalışma, İzmir İli’nin Bozdağ ve Ödemiş ilçelerinden 2017 ve 2018 yılları patates yetiştiriciliği sezonlarında alınan toprak örneklerinin %96'sının G. rostochiensis ile enfekte olduğunu göstermiştir. Bulaşık alanlardaki ortalama kist sayısı, 0.01 ila 3.70 kist g-1 toprak arasında değişmiştir. Globodera rostochiensis'in ikinci dönem larva ve kistlerinin morfolojik ve morfometrik karakterlerinin incelenmesi, Bozdağ ve Ödemiş’ten elde edilen popülasyonlarda arasındaki hafif farklılıkları ortaya koymuştur. Tanımlamanın doğruluğunu değerlendirmek için, tüm popülasyonlar için kısmi ribozomal DNA sekansları amplifiye edilmiş, sekanslanmış ve GenBank veritabanına kaydedilmiştir. Morfolojik ölçümler ve filogenetik analizler sonucunda Bozdağ ve Ödemiş'ten elde edilen popülasyonlar arasında küçük farklılıkların olduğu belirlenmiştir. Sekansların GenBank'ta mevcut karşılık gelen G. rostochiensis popülasyonlarıyla karşılaştırılması sonucunda nükleotid benzerliği %99.89-100 oranında görülmüştür. Bu çalışmanın sonuçları, bu zararlıyı daha etkili bir şekilde karantinaya almak için nematodun fizyolojisini, ekolojisini ve biyolojisini daha iyi anlamaya yardımcı olacaktır.وصف الملف: application/pdf
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83eb7fbe843e8c706f8561ac3167d2ab
https://doi.org/10.16970/entoted.740223 -
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المؤلفون: Riccardo Baroni, Alessandra Zambonelli, Mirco Iotti, Elena Salerni, Federico Puliga, Pamela Leonardi, Claudia Perini
المساهمون: Leonardi, Pamela, Baroni, Riccardo, Puliga, Federico, Iotti, Mirco, Salerni, Elena, Perini, Claudia, Zambonelli, Alessandra
مصطلحات موضوعية: 0106 biological sciences, Plant Science, Biology, 010603 evolutionary biology, 01 natural sciences, Tuber spp, Ascomycota, Tuber aestivum, Mycorrhizae, Botany, Genetics, Colonization, Soil DNA amplification, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Mycelium, Italian white truffle, ITS primers, Soil mycelia, Italy, Soil Microbiology, Truffle, Co-occurrence, General Medicine, biology.organism_classification, Northern italy, Tuber spp. · Italian white truffle · Soil mycelia · Soil DNA amplification · ITS primers, Tuber magnatum, Genus Tuber, 010606 plant biology & botany
الوصف: Tuber magnatum (the white truffle) is the most precious species of the genus Tuber which comprises the hypogeous ectomycorrhizal species called “true truffle.” Despite its high economic value, the knowledge on its ecology is scant, principally due to the difficulty to find its mycorrhizas in the soil. The possibility to detect its mycelium by DNA extracted from soil has given a new chance for studying this truffle species. In this work, the co-occurrence of other Tuber species with T. magnatum mycelium was investigated by using species-specific primers in several productive areas located in central and northern Italy. Most (82%) of the examined soil samples showed at least one other Tuber species in addition to T. magnatum. The most common was T. maculatum (72% of soil samples) followed by T. borchii, T. rufum, T. brumale, T. dryophilum, T. macrosporum, and T. melanosporum (40%, 37%, 22%, 19%, 12%, and 1% of soil samples, respectively). Tuber aestivum was never detected in T. magnatum productive patches. Analysis of species co-occurrence showed that the pairwise associations between T. dryophilum-T. brumale, T. brumale-T. borchii, and T. borchii-T. dryophilum was significant. The results suggest that Tuber mycelial network in white truffle grounds is much more extensive than the distribution of their ectomycorrhizas and competitive exclusion between different Tuber species seems to take place only for root colonization.
وصف الملف: ELETTRONICO
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00ab3e994c3a93bdf11f3463b876023c
http://hdl.handle.net/11697/171171 -
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المؤلفون: Annette Pfitzner-Friedrich, Gabriela Knubben-Schweizer, Christina Strube, Andreas W. Oehm, Cornelia Silaghi, Alexander Stoll
المصدر: Parasites & Vectors, Vol 12, Iss 1, Pp 1-9 (2019)
Parasites & Vectorsمصطلحات موضوعية: 0301 basic medicine, 040301 veterinary sciences, Biopsy, Cattle Diseases, Biology, Polymerase Chain Reaction, Sensitivity and Specificity, law.invention, lcsh:Infectious and parasitic diseases, Electron Transport Complex IV, 0403 veterinary science, 03 medical and health sciences, Plasmid, law, DNA, Ribosomal Spacer, Animals, lcsh:RC109-216, Internal transcribed spacer, Gene, Microfilariae, Polymerase chain reaction, Filarioidea, Skin, Filarial nematode, Cloning, Methodology, Exudates and Transudates, 04 agricultural and veterinary sciences, Molecular biology, cox1, Filariasis, genomic DNA, Parafilaria bovicola, 030104 developmental biology, Infectious Diseases, PCR, Molecular Diagnostic Techniques, Parasitology, Cattle, ITS, Primer (molecular biology)
الوصف: Background Parafilaria bovicola (Nematoda: Filariidae) causes cutaneous bleedings in bovine species. Flies serve as intermediate hosts. In recent years, reports on bovine parafilariosis have become more frequent, corroborating the necessity of reliable diagnostic interventions especially since no molecular or serological test has been available. We aimed to establish a polymerase chain reaction assay to detect DNA of P. bovicola in flies, skin biopsies and serohemorraghic exudates of bleeding spots. Methods PCRs targeting the cytochrome c oxidase subunit 1 (cox1) gene and the internal transcribed spacer region (ITS) of the ribosomal RNA gene cluster were evaluated for their diagnostic sensitivity as well as performance and specificity on biopsy and serohemorrhagic exudate samples from P. bovicola-infected cattle. Results Using serohemorrhagic exudates (n = 6), biopsies (n = 2) and flies (n = 1), the PCR targeting the cox1 gene resulted in a gel band of almost 700 bp. Cloning, sequencing, and removal of primer sequences yielded a 649-bp fragment of the P. bovicola cox1 gene. The PCR targeting the ITS region showed a band of about 1100 bp. Cloning, sequencing, and removal of primer sequences resulted in a 1083 bp stretch of the P. bovicola ITS region. Testing samples from presumably affected animals, the cox1-PCR resulted in bands with the expected size and they were all confirmed as P. bovicola by sequencing. In contrast, the ITS-PCR proved to be less sensitive and less specific and additionally amplified the ITS region of Musca domestica or buttercup DNA. When analysing for sensitivity, the cox1-PCR yielded visible bands up to 2 ng of genomic DNA, whereas the ITS-PCR produced bands up to 3 ng. In a plasmid dilution series, the minimum number of target DNA copies was 102 for the cox1-PCR and 101 in the ITS-PCR. Conclusions The evaluated cox1-PCR enables reliable detection of P. bovicola DNA in skin biopsies and serohemorrhagic exudates. This PCR and, to a limited extent, the ITS-PCR, may help evaluate different therapeutic approaches. Furthermore, the cox1-PCR may be useful for epidemiological studies on the geographical distribution of P. bovicola. Further understanding of the epidemiology of this parasite will help develop and implement effective control strategies.
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المؤلفون: Rui-Qing Ji, Tie-Zheng Wei, Yong-Ping Fu, Bálint Dima, Jun-Liang Chen, Yu Li, Ke Wang, Meng-Le Xie, Chayanard Phukhamsakda
المصدر: PeerJ, Vol 9, p e11982 (2021)
PeerJمصطلحات موضوعية: Biogeography, Mycology, General Biochemistry, Genetics and Molecular Biology, New taxon, Botany, Agaricales, Molecular Biology, Phylogeny, Taxonomy, biology, Phylogenetic tree, General Neuroscience, Biodiversity, General Medicine, biology.organism_classification, Cortinarius, Myxacium, Lithocarpus, Basidiocarp, Key (lock), Medicine, Taxonomy (biology), ITS, General Agricultural and Biological Sciences
الوصف: Cortinarius subsalor and C. tibeticisalor, belonging to the section Delibuti, are described from China as new to science. Cortinarius subsalor has been found to be associated with Lithocarpus trees in subtropical China and resembling C. salor, but it differs from the later by having slender basidiomata and the narrower basidiospores. Cortinarius tibeticisalor was collected from eastern Tibetan Plateau, associated with Abies. It differs from other species within sect. Delibuti by having olive tinge of mature or dried basidiomata and bigger basidiospores. The molecular data also support C. subsalor and C. tibeticisalor as new species. The phylogenetic analyses and biogeography of sect. Delibuti are discussed and a key to the species of this section currently known in the world is provided.
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المؤلفون: Mariela M Moreno, Gerardo E Medina, Mario L Iza, Byron G Fuertes, Celso G Recalde, Marco Cerna
المصدر: Anais da Academia Brasileira de Ciências, Vol 93, Iss 3 (2021)
Anais da Academia Brasileira de Ciências v.93 n.3 2021
Anais da Academia Brasileira de Ciências
Academia Brasileira de Ciências (ABC)
instacron:ABC
Anais da Academia Brasileira de Ciências, Volume: 93, Issue: 3, Article number: e20200297, Published: 13 AUG 2021مصطلحات موضوعية: Science, Biodiversity, Dracula cordobae, phylogeny, Genus, orchids, molecular biology, Clade, Orchidaceae, Ecosystem, Multidisciplinary, geography.geographical_feature_category, biology, Ecology, Dracula, biology.organism_classification, Neotropical, Phylogeography, Geography, Andean region, Epiphyte, ITS, Mountain range
الوصف: The tropical Andes constitute a natural barrier between the Pacific Ocean and the Atlantic; in these mountains, are a great variety of Ecosystems, defined by factors such as orography, winds, humidity, temperature, among others. Some of these Ecosystems have different environmental conditions from tropical ones. In them, there is a great Biodiversity, in some cases endemic and associated with relatively small geographic areas. An example of this biodiversity is the orchids of the genus Dracula, about which discussions are currently generated due to the difficulty in classifying their members. The present work shows a study where DNA was isolated and sequenced from plant samples obtained from 52 species of orchids of the genus Dracula, which were analyzed using the MEGA7 software. Phylogenetic analysis of the DNA sequences showed a well-resolved topology that reflects a geographical pattern of several major clades of the Pacific and Atlantic watersheds. Geophysical conditions of the Andes have generated greater biodiversity of the genus Dracula on the side of the Pacific. Although the species Dracula cordobae and alessandroi reported on both sides of the study site belong to the same clade and show limited mobility through the drier area to the South of the mountain range.
وصف الملف: text/html
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bc573409b26c232e99aca3d49a3591d
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000500808&tlng=en -
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المؤلفون: Shuiquan Tang, Alexander M. Reiter, Janina A Krumbeck, Young Joo Kim, Aishani Prem, Tonatiuh Melgarejo, Annika Linde, James C Pohl
المصدر: Pathogens, Vol 10, Iss 904, p 904 (2021)
Pathogens
Volume 10
Issue 7مصطلحات موضوعية: Microbiology (medical), 040301 veterinary sciences, microbiome, Disease, Bergeyella zoohelcum, Article, Microbiology, 0403 veterinary science, 03 medical and health sciences, fungal ITS, Immunology and Allergy, Microbiome, feline stomatitis, 16S rRNA, Molecular Biology, 030304 developmental biology, 0303 health sciences, CATS, General Immunology and Microbiology, biology, Bacteriome, next-generation DNA sequencing, 04 agricultural and veterinary sciences, biology.organism_classification, oral microbiota, Infectious Diseases, Medicine, Malassezia, Oral Microbiome, mycobiota, Cladosporium
الوصف: Previous studies have suggested the involvement of viral and bacterial components in the initiation and progression of feline chronic gingivostomatitis (FCGS), but the role of fungi remains entirely unknown. This pilot study aimed to investigate the bacteriome and mycobiome in feline oral health and disease. Physical exams, including oral health assessment, of privately owned, clinically healthy (CH) cats (n = 14) and cats affected by FCGS (n = 14) were performed. Using a sterile swab, oral tissue surfaces of CH and FCGS cats were sampled and submitted for 16S rRNA and ITS-2 next-generation DNA sequencing. A high number of fungal species (n = 186) was detected, with Malassezia restricta, Malassezia arunalokei, Cladosporium penidielloides/salinae, and Aspergillaceae sp. being significantly enriched in FCGS samples, and Saccharomyces cerevisiae in CH samples. The bacteriome was significantly distinct between groups, and significant inter-kingdom interactions were documented. Bergeyella zoohelcum was identified as a potential biomarker of a healthy feline oral microbiome. These data suggest that fungi might play a role in the etiology and pathogenesis of FCGS, and that oral health should not simply be regarded as the absence of microbial infections. Instead, it may be viewed as the biological interactions between bacterial and fungal populations that coexist to preserve a complex equilibrium in the microenvironment of the mouth. Additional investigations are needed to improve our understanding of the feline oral ecosystem and the potential interactions between viruses, bacteria, and fungi in FCGS.
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