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1دورية أكاديمية
المؤلفون: Bora Nam, Hyosun Park, Young Lim Lee, Younseo Oh, Jinsung Park, So Yeon Kim, Subin Weon, Sung Hoon Choi, Jae-Hyuk Yang, Sungsin Jo, Tae-Hwan Kim
المصدر: International Journal of Molecular Sciences, Vol 21, Iss 24, p 9771 (2020)
مصطلحات موضوعية: osteoblast differentiation, mineralization, TGFβ1, SMURF1, C/EBPβ, DKK1, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Transforming growth factor β1 (TGFβ1) is a major mediator in the modulation of osteoblast differentiation. However, the underlying molecular mechanism is still not fully understood. Here, we show that TGFβ1 has a dual stage-dependent role in osteoblast differentiation; TGFβ1 induced matrix maturation but inhibited matrix mineralization. We discovered the underlying mechanism of the TGFβ1 inhibitory role in mineralization using human osteoprogenitors. In particular, the matrix mineralization-related genes of osteoblasts such as osteocalcin (OCN), Dickkopf 1 (DKK1), and CCAAT/enhancer-binding protein beta (C/EBPβ) were dramatically suppressed by TGFβ1 treatment. The suppressive effects of TGFβ1 were reversed with anti-TGFβ1 treatment. Mechanically, TGFβ1 decreased protein levels of C/EBPβ without changing mRNA levels and reduced both mRNA and protein levels of DKK1. The degradation of the C/EBPβ protein by TGFβ1 was dependent on the ubiquitin–proteasome pathway. TGFβ1 degraded the C/EBPβ protein by inducing the expression of the E3 ubiquitin ligase Smad ubiquitin regulatory factor 1 (SMURF1) at the transcript level, thereby reducing the C/EBPβ-DKK1 regulatory mechanism. Collectively, our findings suggest that TGFβ1 suppressed the matrix mineralization of osteoblast differentiation by regulating the SMURF1-C/EBPβ-DKK1 axis.
وصف الملف: electronic resource
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المؤلفون: Woo Nam, Jae-Hun Sim, Soo-Dong Park, Jisoo Kim, Hyeonji Kim, Chu Hyun Bae, Bora Nam, Jung-Lyoul Lee, Jooyun Kim
المصدر: Journal of Medicinal Food. 24:569-576
مصطلحات موضوعية: 0301 basic medicine, Allergy, Ovalbumin, Panax, Medicine (miscellaneous), Inflammation, Pharmacology, Immunoglobulin E, Mice, 03 medical and health sciences, Ginseng, 0302 clinical medicine, Immune system, medicine, Animals, Interleukin 4, Mice, Inbred BALB C, 030109 nutrition & dietetics, Nutrition and Dietetics, biology, Chemistry, Interleukin, medicine.disease, Rhinitis, Allergic, Disease Models, Animal, 030220 oncology & carcinogenesis, biology.protein, Cytokines, Interleukin-4, Fermented Foods, medicine.symptom
الوصف: Ginseng (the root of Panax ginseng Meyer) has been reported to have many biologic therapeutic effects, including anti-inflammatory properties, and ginsenosides are considered as one of the factors responsible for these therapeutic effects. To improve their therapeutic action, probiotic bacteria are used to ferment and chemically transform ginsenosides in red ginseng (RG). In this study, we aimed to investigate the beneficial effects of RG fermented by probiotic bacteria (FRG) against ovalbumin (OVA)-induced allergic rhinitis in a mouse model. We induced the mouse model via OVA inhalation; experimental results revealed increased immunoglobulin E (IgE) and interleukin (IL)-4 levels, leading to Th2-type cytokine response. The mice with induced allergy were then orally administered RG and FRG over 2 weeks, as a result of which, IL-4 and IgE levels in bronchoalveolar lavage fluid, nasal fluid, and serum were found to be ameliorated more effectively by FRG than by RG, suggesting that FRG has better immune regulatory effects than RG. FRG also downregulated immune cell levels, such as those of eosinophils and basophils, and significantly decreased the thickness of OVA-induced respiratory epithelium compared to RG. Collectively, the results showed that FRG treatment alleviates inflammation, thereby extending a protective effect to mice with OVA-induced inflammatory allergic rhinitis.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5bba1053bd56bfd9d8074cb7b2a6516
https://doi.org/10.1089/jmf.2020.4854 -
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المؤلفون: Wanook Kim, Kiwoong Lee, Jung Young Kim, Woonghee Lee, Sanghwan Ko, Kyo Chul Lee, Kondapa Naidu Bobba, Abhinav Bhise, Sang Taek Jung, Hye Jin Lee, Jeongsoo Yoo, Bora Nam, Hyun S. Park, Subramani Rajkumar, Sang Hyuk Lee
المصدر: Journal of Materials Chemistry B. 9:2993-2997
مصطلحات موضوعية: Biomedical Engineering, Breast Neoplasms, Pharmacology, Polyethylene Glycols, 03 medical and health sciences, Antineoplastic Agents, Immunological, 0302 clinical medicine, Trastuzumab, PEG ratio, medicine, Humans, General Materials Science, 030304 developmental biology, Immuno pet, 0303 health sciences, High contrast, biology, Chemistry, General Chemistry, General Medicine, Positron-Emission Tomography, 030220 oncology & carcinogenesis, PEGylation, biology.protein, Female, Radiopharmaceuticals, Antibody, Linker, medicine.drug, Conjugate
الوصف: The prolonged blood circulation of the radiolabeled antibody conjugates is problematic when using immuno-PET imaging due to the increased radiation exposure and longer hospitalization required until sufficient contrast develops. In contrast to the prevailing belief that PEGylation prolongs blood retention time, we observed that a PEGylated antibody with a short PEG8 linker cleared much faster from the blood while maintaining tumor uptake compared to its non-PEGylated counterpart. Breast tumors were clearly visualized with a very high tumor-to-background ratio as early as 24 h after injection in immuno-positron emission tomography (PET) imaging.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a695f91c3071c0d8712a739f508239c
https://doi.org/10.1039/d0tb02911d -
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المؤلفون: Jung-Lyoul Lee, Jae-Hun Sim, Bora Nam, Hyeon Ji Kim, Chu Hyun Bae, Woo Nam, Soo-Dong Park, Joo Yun Kim, Jisoo Kim
المصدر: Preventive Nutrition and Food Science. 25:158-165
مصطلحات موضوعية: 0303 health sciences, Nutrition and Dietetics, 030309 nutrition & dietetics, digestive, oral, and skin physiology, Mucin, Pharmacology, digestive system diseases, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, medicine.anatomical_structure, chemistry, 030220 oncology & carcinogenesis, Gastric mucosa, medicine, Gastric acid, Cyclic adenosine monophosphate, Secretion, Receptor, Histamine, Food Science, Gastrin
الوصف: Cudrania tricuspidata has been used as an East Asian folk remedy to treat various symptoms. Recently, scientific evidence of the efficacy of C. tricuspidata has emerged. The objective of this study was to elucidate protective role of C. tricuspidata in the gastric mucosa using pylorus-ligated Sprague-Dawley rats and primary parietal cells. C. tricuspidata ethanol extracts attenuated gastric mucosal damage, secretion, and juice acidity in pylorus-ligated rats; however, it did not affect expression of gastric acid-related genes [muscarinic acetylcholine receptor M3 receptor (M3R), histamine H2-receptors (H2R), and cholecystokinin-2/gastrin receptors (CCK2R)] or serum gastrin concentrations. Furthermore, extracts greatly reduced levels of gastric cyclic adenosine monophosphate (cAMP) and significantly increased mRNA levels of gastric-type mucins (MUC5AC and MUC6). To identify the mode of action of C. tricuspidata extract in regulating gastric acid secretion, intracellular cAMP and mRNA for H2R, M3R, and CCK2R were measured in primary parietal cells. mRNA levels of H2R, M3R, and CCK2R did not significantly differ following treatment with C. tricuspidata extract, whereas cAMP induced by the H2R-specific agonist was significantly decreased. C. tricuspidata may therefore reduce gastric acid secretion by inhibiting H2R activity rather than regulating mRNA expression. These finding suggest that ethanol extracts of C. tricuspidata inhibit H2R-related gastric acid secretion and increase gastric mucus to help prevent gastric mucosal damage. Therefore, C. tricuspidata extract has potential to be used in foods and medicines to prevent diseases related to gastric mucosal damage, such as gastritis and functional dyspepsia.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::191df1c838489dd83fea394c21886a42
https://doi.org/10.3746/pnf.2020.25.2.158 -
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المؤلفون: Yong-Gil Kim, Jinsung Park, Bora Nam, Jae Hyuk Yang, Hyosun Park, Young Lim Lee, Subin Weon, Tae-Hwan Kim, Sungsin Jo
المصدر: Experimental and Therapeutic Medicine
مصطلحات موضوعية: Cancer Research, medicine.medical_specialty, Hyaline cartilage, Chemistry, Inflammatory arthritis, chondrocytes, Articles, General Medicine, Osteoarthritis, medicine.disease, Chondrogenesis, Chondrocyte, medicine.anatomical_structure, Endocrinology, Immunology and Microbiology (miscellaneous), anti-TNFα inhibitor, Internal medicine, TNFα, medicine, Synovial fluid, chondrogenic differentiation, inflammatory arthritis, metabolic shift, Aggrecan, Hyaline
الوصف: Because damage to hyaline cartilage is irreversible, relieving progressive cartilage destruction is an important therapeutic approach for inflammatory arthritis. In the present study, human hyaline chondrocytes were isolated from total knee replacements of 15 patients with osteoarthritis (OA) and three with rheumatoid arthritis (RA). Synovial fluid of OA (n=25) and RA (n=34) were collected to measure tumor necrosis factor α (TNFα) using ELISA. Consistent with previous studies, the synovial fluid exhibited high TNFα levels and hyaline cartilage was severely destroyed in patients with RA. TNFα-treated chondrocytes were used as model for inflammatory arthritis. TNFα did not influence proliferation or extracellular matrix expression in chondrocytes, but induced matrix metalloproteinase (MMP)1, 3 and 13 expression levels in chondrocytes, which was accompanied by activation of nuclear factor-κB signaling. During chondrogenic differentiation, TNFα attenuated mRNA expression levels of anabolic factors (collagen type 2 and aggrecan) and enhanced mRNA expression of catabolic factors (MMP1, MMP3 and MMP13) in chondrocytes. Moreover, anti-TNFα agents (Golimumab) inhibited the TNFα-induced metabolic shift in chondrocytes and chondrogenic differentiation. The present study revealed a mechanism by which TNFα may induce metabolic shift in chondrocytes, leading to progressive chondrocyte destruction.
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المؤلفون: Jae-Hun Sim, Joo Yun Kim, Jang Sung Sik, Jeung Woon Hee, Hyeon Ji Kim, Soo-Dong Park, Bora Nam, Jung-Lyoul Lee, Woo Nam
المصدر: Preventive Nutrition and Food Science. 24:136-143
مصطلحات موضوعية: 0301 basic medicine, Normal diet, 030209 endocrinology & metabolism, Gut flora, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Oral administration, medicine, Food science, Nutrition and Dietetics, biology, Adiponectin, Leptin, digestive, oral, and skin physiology, nutritional and metabolic diseases, food and beverages, biology.organism_classification, medicine.disease, Obesity, Lactic acid, 030104 developmental biology, chemistry, hormones, hormone substitutes, and hormone antagonists, Lactobacillus plantarum, Food Science
الوصف: Obesity is a major health issue worldwide, and is associated with many diseases including type 2 diabetes. In this study, we evaluated the anti-obesity effects of combinations of two lactic acid bacteria (LAB), Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032, and Cinnamomi Ramulus (CR) extract, and explored the mechanism through which they modulate gut microbiota using diet-induced obese mice. Male C57BL/6J mice were randomly divided into five groups that received a high-fat diet (HFD), HFD and LAB (HFD+LAB), HFD and CR extract (HFD+CR), HFD with LAB and CR extract (HFD+LAB+CR), or normal diet for 10 weeks. The mice in the HFD+LAB+CR group showed significant reductions in body weight gain, in particular epididymal fat and liver, blood leptin levels, and an increase in the levels of blood adiponectin. In addition, the LAB and CR extract altered the gut microbiota, mainly increasing the alpha diversity. These results demonstrate that a mixture of two LAB (Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032) and CR extracts alleviate HFD-induced obesity, and has potential of being used as a strategy for the treatment of obesity.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::b39e04d6f744ce759ffa88864544bc5d
https://doi.org/10.3746/pnf.2019.24.2.136 -
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المؤلفون: Bora Nam, Woo Nam, Jae-Hun Sim, Soo A Kim, Jung-Lyoul Lee, Soo-Dong Park, Jang Sung Sik, Jeung Woon Hee
المصدر: Preventive Nutrition and Food Science
مصطلحات موضوعية: 0303 health sciences, Nutrition and Dietetics, Myosin light-chain kinase, tight junctions, biology, Tight junction, 030306 microbiology, Chemistry, Inflammation, Articles, Pharmacology, biology.organism_classification, Occludin, anti-inflammation, lactic acid bacteria, 03 medical and health sciences, Mrna level, Caco-2, medicine, Tumor necrosis factor alpha, medicine.symptom, Caco-2 cells, Lactobacillus plantarum, 030304 developmental biology, Food Science
الوصف: In addition to intestinal balance, probiotics are known to have beneficial effects on skin inflammation, metabolic diseases, and emotions. Previously, we have reported the skin anti-aging effects of Lactobacillus plantarum HY7714 (HY7714) in a clinical trial. To prove the protective skin effects of HY7714 through the intestinal tight junction (TJ), we investigated the effects of HY7714 on the intestines through tumor necrosis factor (TNF)-α induced TJ defects in Caco-2 cells. Specifically, 24 h treatment with HY7714 restored the decreased expression of zonula occludens-1, occludin, and claudin-1 compared to the TNF-α-treated groups (P
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::755f5d9cbe2aa18fee7284aacc562040
http://europepmc.org/articles/PMC6456235 -
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المؤلفون: Young Lim Lee, Sung Hoon Choi, Tae-Hwan Kim, So Yeon Kim, Jae Hyuk Yang, Bora Nam, Hyosun Park, Sungsin Jo, Subin Weon, Younseo Oh, Jinsung Park
المصدر: International Journal of Molecular Sciences, Vol 21, Iss 9771, p 9771 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 24مصطلحات موضوعية: musculoskeletal diseases, Ubiquitin-Protein Ligases, SMAD, Catalysis, Article, Inorganic Chemistry, Transforming Growth Factor beta1, lcsh:Chemistry, Calcification, Physiologic, Ubiquitin, Osteogenesis, medicine, Humans, mineralization, Physical and Theoretical Chemistry, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, Aged, Messenger RNA, Osteoblasts, biology, Chemistry, DKK1, CCAAT-Enhancer-Binding Protein-beta, Organic Chemistry, TGFβ1, Osteoblast, Cell Differentiation, General Medicine, Computer Science Applications, Ubiquitin ligase, Cell biology, Extracellular Matrix, medicine.anatomical_structure, Gene Expression Regulation, lcsh:Biology (General), lcsh:QD1-999, osteoblast differentiation, SMURF1, C/EBPβ, biology.protein, Osteocalcin, Intercellular Signaling Peptides and Proteins, Transforming growth factor
الوصف: Transforming growth factor &beta
1 (TGF&beta
1) is a major mediator in the modulation of osteoblast differentiation. However, the underlying molecular mechanism is still not fully understood. Here, we show that TGF&beta
1 has a dual stage-dependent role in osteoblast differentiation
TGF&beta
1 induced matrix maturation but inhibited matrix mineralization. We discovered the underlying mechanism of the TGF&beta
1 inhibitory role in mineralization using human osteoprogenitors. In particular, the matrix mineralization-related genes of osteoblasts such as osteocalcin (OCN), Dickkopf 1 (DKK1), and CCAAT/enhancer-binding protein beta (C/EBP&beta
) were dramatically suppressed by TGF&beta
1 treatment. The suppressive effects of TGF&beta
1 were reversed with anti-TGF&beta
1 treatment. Mechanically, TGF&beta
1 decreased protein levels of C/EBP&beta
without changing mRNA levels and reduced both mRNA and protein levels of DKK1. The degradation of the C/EBP&beta
protein by TGF&beta
1 was dependent on the ubiquitin&ndash
proteasome pathway. TGF&beta
1 degraded the C/EBP&beta
protein by inducing the expression of the E3 ubiquitin ligase Smad ubiquitin regulatory factor 1 (SMURF1) at the transcript level, thereby reducing the C/EBP&beta
DKK1 regulatory mechanism. Collectively, our findings suggest that TGF&beta
1 suppressed the matrix mineralization of osteoblast differentiation by regulating the SMURF1-C/EBP&beta
DKK1 axis.وصف الملف: application/pdf
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المؤلفون: Hoseob Chang, Min-Cheol Kim, Ji-Yeon Park, Bora Nam, Deog-Keun Kim
المصدر: Applied biochemistry and biotechnology. 193(2)
مصطلحات موضوعية: 0106 biological sciences, Hot Temperature, Chlorella vulgaris, Bioengineering, 01 natural sciences, Applied Microbiology and Biotechnology, Biochemistry, Hydrothermal circulation, Surface-Active Agents, Pulmonary surfactant, 010608 biotechnology, Molecular Biology, chemistry.chemical_classification, 010405 organic chemistry, Chemistry, Extraction (chemistry), Fatty Acids, Cationic polymerization, Fatty acid, General Medicine, 0104 chemical sciences, Yield (chemistry), Biofuels, Biotechnology, Nuclear chemistry
الوصف: The feasibility of surfactants for enhancement of extraction efficiencies in wet oil extraction through an acidic hydrothermal process was evaluated. Three different types of surfactants were tested: anionic (SDBS and SDS), cationic (CTAB and MBC), and non-ionic (IGEPAL CA-210 and Tween 60). The total fatty acid content of Chlorella vulgaris was 291.0 mg/g cell. Under the no-surfactant condition, the oil-extraction yield of the acidic hydrothermal extraction was 75.5%. The addition of SDBS and MBC at the 0.4% concentration showed enhanced oil-extraction performance, 85.4 and 85.7% yields, respectively. CTAB and Tween 60 showed low extraction yields, less than 43.0%. SDS and IGEPAL CA-210 showed high oil-extraction yields, higher, in fact, than the initial fatty acid content, due to surfactant partitioning into microalgal oil. With increasing surfactant concentration, the oil-extraction yields of CTAB decreased, those of IGEPAL CA-210 gradually increased, and those of SDBS increased and then decreased again. The best performance, an oil-extraction yield of 95.6%, was observed under the 0.2% SDBS, 120 °C, 1 h condition. Although IGEPAL CA-210 showed the high net oil-extraction yield of 98.3% at the 0.6% surfactant concentration, 61.2% of surfactant was partitioned into oil. Graphical abstract.
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المؤلفون: Sungsin Jo, Jeehee Youn, Tae Hwan Kim, Ye Soo Park, Seunghun Lee, Bora Nam, Eunju Lee, Juyeon Kang, Yong-Gil Kim
المصدر: Arthritis Research & Therapy
Arthritis Research & Therapy, Vol 22, Iss 1, Pp 1-7 (2020)مصطلحات موضوعية: 0301 basic medicine, Male, medicine.medical_specialty, lcsh:Diseases of the musculoskeletal system, beta-Glucans, Osteoprogenitors, medicine.medical_treatment, 03 medical and health sciences, chemistry.chemical_compound, Mice, 0302 clinical medicine, Spinal ankylosis, Internal medicine, medicine, Animals, Humans, Spondylitis, Ankylosing, Testosterone, Osteoblastic activity, 030203 arthritis & rheumatology, Ankylosing spondylitis, Osteoblasts, Curdlan-administered SKG mice, biology, business.industry, Osteoblast, Dihydrotestosterone, Dutasteride, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, Cytokine, Endocrinology, chemistry, Osteocalcin, biology.protein, Sclerostin, lcsh:RC925-935, business, medicine.drug, Research Article
الوصف: Background Ankylosing spondylitis (AS) is characteristically male-predominant, and progressive spinal ankylosis affects male patients more severely; however, the hormonal effects in males with AS are poorly understood. Methods In the present study, the regulatory effects of dutasteride, a 5-α reductase inhibitor that blocks the conversion of testosterone to dihydrotestosterone (DHT), were examined in curdlan-administered male SKG mice to determine spinal bone formation, bone metabolism-related markers, and interleukin (IL)-17A cytokine and T cell populations. In addition, the effects of DHT on primary osteoprogenitors from the facet joints of AS patients were assessed based on osteoblast-related parameters. DHT level was measured, and the correlation with modified Stoke Ankylosing Spondylitis Spinal Score (mSASSS) was analyzed in AS patients. Results In curdlan-administered SKG mice, dutasteride treatment resulted in an increased accumulation of hydroxyapatite in the spine which was positively correlated with serum IL-17A levels. In the analysis of bone metabolism-related molecules, a decrease in sclerostin levels was observed in the sera in the dutasteride group. Continuous exposure to DHT resulted in fewer calcium deposits in AS osteoprogenitors during osteoblast differentiation. DHT-treated AS osteoprogenitors showed decreased osteocalcin and increased DKK1 and SOST1 mRNA expression, supporting the results of the in vivo experiments. Treatment with dutasteride upregulated bone formation in the spine of curdlan-administered SKG mice and DHT treatment downregulated osteoblast differentiation in vitro. Conclusions Treatment with dutasteride affected the bone formation in the spine of curdlan-treated SKG mice, and DHT treatment attenuated osteoblast differentiation in vitro. Therefore, contrary to what could be expected if osteoblasts contributed to spinal ankylosis, DHT inhibition might increase rather than decrease the progression of spinal ankylosis despite the higher levels of DHT observed in many AS patients.
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