المؤلفون: Silke Messerschmidt, Miriam Heinrich, Kim Gruber, Franziska Grunewald, Manuel Garcia-Käufer, Carsten Gründemann, Florian C. Stintzing, Carmen Steinborn, Christiane Beckmann, Roman Huber, Matthias Knoedler, Peter Lorenz, Anja Herrick

المصدر: Journal of Ethnopharmacology. 194:642-650

مصطلحات موضوعية: 0301 basic medicine, Allergy, Cell Degranulation, medicine.medical_treatment, Pharmacology, 01 natural sciences, Quercus, 03 medical and health sciences, Tandem Mass Spectrometry, Drug Discovery, Animals, Humans, Medicine, Tannin, Mast Cells, chemistry.chemical_classification, Traditional medicine, Plant Extracts, business.industry, 010401 analytical chemistry, Degranulation, Allergens, medicine.disease, Basophils, Rats, 0104 chemical sciences, Basophilic, 030104 developmental biology, Cytokine, chemistry, Polyphenol, visual_art, Plant Bark, visual_art.visual_art_medium, Bark, business, Chromatography, Liquid

الوصف: Ethnopharmacological relevance Oak bark has been used since ancient times in Europaen ethnomedicine because of its adstringent, antimicrobial and hemostatic features, e.g. as a remedy for the treatment of wounds and skin diseases. Purpose Oak bark tannins are considered as bioactive natural products, interacting with surface proteins of mucous membranes and might be beneficial for the treatment of allergic diseases. This study investigated the effect of an oak bark decoction (OBD) and isolated tannin fractions on the degranulation capacity and cytokine/chemokine release from rat basophilic cells and human mast cells in vitro, which are essential for the initiation of early- and late-phase allergic reactions. Methods and methods By chromatographic separation on Sephadex® LH-20 high- and low-molecular weight tannins were separated from OBD and the tannin composition analyzed by HPLC(DAD)-MSn. Then, the OBD and its fractions were tested in degranulation (β-hexosaminidase activity) of allergen-specific-activated basophilic cells in a photometric assay. Results The OBD and the high-molecular tannin fraction showed a dose-dependent inhibition of cell degranulation. Furthermore, the OBD and particularly its high molecular weight tannin fraction exhibited an inhibitory activity on the IL-8-, IL-6- and TNF-α-secretion from stimulated human mast cells, detected and quantified by ELISA. Conclusion The OBD and its high-molecular weight tannins revealed an impact on allergic mediator release of basophilic cells and human mast cells and thereby provide a rationale for the topical treatment with OBD preparations.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::316c9d9079efc3c607cef6abfa90d912
https://doi.org/10.1016/j.jep.2016.10.027

المؤلفون: Carsten Gründemann, Christiane Beckmann, Florian C. Stintzing, Roman Huber, Ulrich Meyer, Daniel Briemle

المصدر: Planta Medica. 78:334-340

مصطلحات موضوعية: Citrus, Cell Survival, Pharmaceutical Science, Cell Line, Analytical Chemistry, chemistry.chemical_compound, food, Anti-Allergic Agents, Drug Discovery, Hydroxybenzoates, medicine, Animals, Humans, Immunologic Factors, Mast Cells, Rosaceae, Pharmacology, Neochlorogenic acid, Traditional medicine, Plant Extracts, Organic Chemistry, Degranulation, food and beverages, Azelastine, food.food, In vitro, Basophils, Rats, Citrus medica, Basophilic, Complementary and alternative medicine, chemistry, Fermentation, Molecular Medicine, Tumor necrosis factor alpha, Eriocitrin, medicine.drug

الوصف: This study aimed to investigate the immunomodulatory and antiallergic properties of preparations from lemon, Citrus medica L. (citrus), and quince, Cydonia oblonga Mill. (Cydonia), which are used in pharmaceutical products to treat patients suffering from allergic disorders. Preparations were analyzed with respect to their impact on the degranulation capacity from basophilic cells as well as mediator release from activated human mast cells in vitro, including IL-8 and TNF- α secretion. The results show that the degranulation of basophilic cells was diminished only in the presence of Citrus, and this effect was compared to the synthetic drug azelastine. Furthermore, Citrus and Cydonia both inhibited the production of IL-8 and TNF- α from human mast cells, and at low concentrations additive effects were observed. As a positive inhibition control, dexamethasone was used. LC-MS analyses showed that the major phenolic components in extracts from Citrus and Cydonia are eriocitrin and neochlorogenic acid, respectively. Nevertheless, these compounds do not show biological effects at concentration levels detected in their corresponding extracts. In conclusion, the present data provide a rational base for the use of the single pharmaceutical preparations from Citrus and Cydonia in a differentiated treatment of allergic disorders in part by the regulation of soluble allergic mediators from basophilic cells and mast cells.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9e54b48a8c604d449fec4f40b7dbfe6
https://doi.org/10.1055/s-0031-1280455

المؤلفون: Florian C. Stintzing, Katrin Menke, Christiane Beckmann, Alexander Schramm, Tycho Jan Zuzak, Harald Stephan, Jennifer Felenda

مصطلحات موضوعية: Pharmacology, Complementary and alternative medicine, Taraxacum officinale, Drug Discovery, Botany, Medizin, Pharmaceutical Science, Molecular Medicine, Dandelion, Biology, Pediatric cancer

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5039adbfc0ea90e4bce6be5dd07ced69

المؤلفون: Florian C. Stintzing, Christiane Beckmann, Anja Herrick

المصدر: Phytomedicine. 22:S24-S25

مصطلحات موضوعية: Pharmacology, Complementary and alternative medicine, Traditional medicine, Drug Discovery, Viscum album, Pharmaceutical Science, Molecular Medicine, Biology, biology.organism_classification

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::494e50106b1fdd8b0ac9dc3eeee95540
https://doi.org/10.1016/j.phymed.2015.05.064

المؤلفون: Claudia vom Hoff-Heise, Cristina Semaca, Georg Seifert, Rebecca Hufnagel, Dietrich Schlodder, Christiane Beckmann, Günter Henze, Alfred Längler, Gabriele Calaminus, Sabine Rieger, Jennifer Felenda

المصدر: Phytomedicine. 22:S18-S19

مصطلحات موضوعية: Pharmacology, Pediatrics, medicine.medical_specialty, Complementary and alternative medicine, Quality of life, business.industry, Family medicine, Drug Discovery, Pharmaceutical Science, Molecular Medicine, Medicine, business

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::bfeb9b57e7fa4e18cbb8ef563f9ac87d
https://doi.org/10.1016/j.phymed.2015.05.035

المؤلفون: Alfred Längler, Sabine Rieger, Jennifer Felenda, Rebecca Hufnagel, Dietrich Schlodder, Gabriele Calaminus, Günter Henze, Claudia vom Hoff-Heise, Christiane Beckmann, Christina Semaca, Georg Seifert

المصدر: Phytomedicine. 22:S18

مصطلحات موضوعية: Pharmacology, medicine.medical_specialty, Treatment intervention, Complementary and alternative medicine, business.industry, Drug Discovery, medicine, Pharmaceutical Science, Molecular Medicine, Intensive chemotherapy, Intensive care medicine, business

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20cf07834325cbc0d2a9e84c307bd340
https://doi.org/10.1016/j.phymed.2015.05.034