المؤلفون: Xu A; State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology (SIMIT), Chinese Academy of Sciences, Shanghai 200050, P. R. China.; College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, P. R. China., He P; State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology (SIMIT), Chinese Academy of Sciences, Shanghai 200050, P. R. China.; College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, P. R. China., Ye C; Academy for Advanced Interdisciplinary Studies and Department of Physics, Southern University of Science and Technology (SUSTech), Shenzhen 518055, P. R. China., Liu Z; State Key Laboratory of Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, P. R. China.; College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, P. R. China., Gu B; Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, P. R. China., Gao B; Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, P. R. China., Li Y; State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology (SIMIT), Chinese Academy of Sciences, Shanghai 200050, P. R. China.; College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, P. R. China., Dong H; State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology (SIMIT), Chinese Academy of Sciences, Shanghai 200050, P. R. China.; College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, P. R. China., Chen D; Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, P. R. China., Wang G; Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, P. R. China., Yang S; State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology (SIMIT), Chinese Academy of Sciences, Shanghai 200050, P. R. China.; College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, P. R. China., Ding G; State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology (SIMIT), Chinese Academy of Sciences, Shanghai 200050, P. R. China.; College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, P. R. China.

المصدر: ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2020 Mar 04; Vol. 12 (9), pp. 10781-10790. Date of Electronic Publication: 2020 Feb 25.

نوع المنشور: Evaluation Study; Journal Article

بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE

مواضيع طبية MeSH: Graphite/*chemistry , Luminescent Measurements/*methods , Neoplasms/*metabolism , Quantum Dots/*chemistry , Reactive Oxygen Species/*analysis, Animals ; Cell Line, Tumor ; Humans ; Luminescence ; Luminescent Measurements/instrumentation ; Mice ; Mice, Inbred BALB C ; Neoplasms/diagnosis ; Oxidation-Reduction ; Phosphorus Compounds/chemistry ; Reactive Oxygen Species/metabolism

مستخلص: The evaluation of intracellular reactive oxygen species (ROS) would greatly deepen the understanding of cell metabolism/proliferation and tumor detection. However, current long-acting level tracking techniques for intracellular ROS remain unsuited to practical applications. To solve this problem, we synthesized cyclotriphosphazene-doped graphene quantum dots (C-GQDs) whose quantum yield is highly sensitive to ROS (increased by 400% from 0.12 to 0.63). Electron cloud polarization of oxidized cyclotriphosphazene rings in C-GQDs is confirmed to account for this novel optical property by density functional theory calculations and experimental results. In combination with excellent biological stability, C-GQDs achieve a long-acting evaluation of intracellular ROS level (more than 72 h) with an accuracy of 98.3%. In addition, recognition rates exceeding 90% are demonstrated to be feasible for eight kinds of tumor cell lines cultured with C-GQDs, which can also be expanded to in vivo detection. C-GQDs also show a high recognition rate (82.33%) and sensitivity (79.65%) for tumor cells in blood samples.

المؤلفون: Min X; School of Information Science and Technology, Xiamen University, Xiamen, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China., Fu D; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China., Zhang J; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China., Zeng J; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China., Weng Z; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China., Chen W; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China., Zhang S; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China., Zhang D; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China. zhangdongxu@xmu.edu.cn.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China. zhangdongxu@xmu.edu.cn.; School of Public Health, Xiamen University, Xiamen, Fujian, China. zhangdongxu@xmu.edu.cn., Ge S; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China. sxge@xmu.edu.cn.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China. sxge@xmu.edu.cn.; School of Public Health, Xiamen University, Xiamen, Fujian, China. sxge@xmu.edu.cn., Zhang J; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China., Xia N; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China.

المصدر: Biomedical microdevices [Biomed Microdevices] 2018 Oct 25; Vol. 20 (4), pp. 91. Date of Electronic Publication: 2018 Oct 25.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Springer US Country of Publication: United States NLM ID: 100887374 Publication Model: Electronic Cited Medium: Internet ISSN: 1572-8781 (Electronic) Linking ISSN: 13872176 NLM ISO Abbreviation: Biomed Microdevices Subsets: MEDLINE

مواضيع طبية MeSH: Lab-On-A-Chip Devices*/economics, Biomarkers/*analysis , Immunoassay/*instrumentation , Luminescent Measurements/*instrumentation, Automation ; Costs and Cost Analysis ; Ferritins/analysis

مستخلص: A rapid, sensitive and quantitative biomarker detection platform is of great importance to the small clinic or point-of-care (POC) diagnosis. In this work, we realize that an automated diagnostic platform mainly includes two components: (1) an instrument that can complete all steps of the chemiluminescence immunoassay automatically and (2) an integrated microfluidic chip which is disposable and harmless. In the instrument, we adopt vacuum suction cups which are driven by linear motor to realize a simple, effective and convenient control. The method of acridine esterification chemiluminescence is adopted to achieve a quantitative detection, and a photomultiplier tube is used to detect photons from acridine ester producing in alkaline conditions. We use the laser cutting machine and hot press machine to accomplish the product of microfluidic chips. The automated microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of ferritin. We observe alinear relationship between CL intensity and the concentration of ferritin from 5.1 to 1300 ng mL ^-1 and the limit of detection (LoD) is 2.55 ng mL ^-1 . At the same time, we also used the automated microfluidics-based system to test clinical serum samples. The whole process of chemiluminescence experiment can complete within 45 min. We realize that this lab-on-a-chip chemiluminescence immunoassay platform with features of automation and quantitation provides a promising strategy for POC diagnosis.

المؤلفون: Wang X; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Chi H; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Li Q; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Li W; Jilin Medical University, Jilin, 132013, China.; Key Laboratory of Preparation and Application of Environmental Friendly Materials, Ministry of Education, Jilin Normal University, Changchun, 130103, China., Li J; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Li B; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Gao W; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Zhang D; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Sun Y; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Yi L; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Qu H; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Wang Y; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China., Li Z; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China. 14758634@qq.com., Xia Z; Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China. ammszipxia@163.com.

المصدر: Molecular imaging and biology [Mol Imaging Biol] 2018 Feb; Vol. 20 (1), pp. 21-26.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 101125610 Publication Model: Print Cited Medium: Internet ISSN: 1860-2002 (Electronic) Linking ISSN: 15361632 NLM ISO Abbreviation: Mol Imaging Biol Subsets: MEDLINE

مواضيع طبية MeSH: Luminescent Measurements*, Anti-Bacterial Agents/*pharmacology , Gram-Negative Bacteria/*drug effects , Plasmids/*metabolism, Animals ; Bacterial Load/drug effects ; Escherichia coli/drug effects ; Female ; Imaging, Three-Dimensional ; Mice, Inbred BALB C ; Microbial Sensitivity Tests ; Organ Specificity/drug effects ; Tigecycline/pharmacology

مستخلص: Purpose: The present study aims to develop five Gram-negative bacteria expressing bacterial luciferase for use to evaluate the influence of different antibiotics on bacterial bioluminescence.
Procedures: The pBBR-lux plasmid was introduced into five Gram-negative bacteria; the bioluminescent signals and colony-forming unit (CFU)/ml of all the bioluminescent strains were monitored with six antibiotics at various concentrations.
Results: Dose-dependent bioluminescence signals can be used for rapid bacterial antibiotic susceptibility test (AST). All five bioluminescent bacterial strains have similar bioluminescence and CFU enhancement at sub-minimum inhibitory concentration (MIC) of six different antibiotics.
Conclusion: The bioluminescent signals and CFU enhancement at sub-MIC antibiotic concentrations should be of value in the research of new antibiotic drugs and bioluminescent imaging.

المؤلفون: Liao Y; MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China., Zhou X; MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China., Fu Y; MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China., Xing D; MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China.

المصدر: Analytical chemistry [Anal Chem] 2017 Dec 05; Vol. 89 (23), pp. 13016-13023. Date of Electronic Publication: 2017 Nov 20.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE

مواضيع طبية MeSH: Biomarkers/*analysis , Coordination Complexes/*chemistry , Electrochemical Techniques/*methods , Luminescent Measurements/*methods , Polymers/*chemistry, Carcinoembryonic Antigen/analysis ; Coordination Complexes/chemical synthesis ; Hepatitis B virus/isolation & purification ; Polymers/chemical synthesis ; RNA, Ribosomal, 16S/analysis ; Ruthenium/chemistry ; Thrombin/analysis

مستخلص: Electrochemiluminescence (ECL) has been engineered to perform various tasks in the area of immunoassays and molecular diagnosis. However, there is still substantial potential for developments of ECL assay with high efficiency to achieve trace analysis. Herein, we demonstrate a polymer-amplified ECL assay via construction of linear Ru(bpy) 3 ²⁺ -polymer. This new polymer material compensates for the relatively low ECL intensity from single ECL luminophore and realizes a stable and controllable labeling process. The polymer-amplified ECL assay achieved a remarkable sensitivity of 100 amol. The wide-ranging applications of the polymer-amplified ECL assay for Hepatitis B virus, carcinoembryonic antigen, 16sRNA, and thrombin also demonstrate its superiority. Hence, the polymer-amplified ECL assay possesses the potential to create a new paradigm in amplified ECL assays that could provide outstanding performance for biomedical analysis.

المؤلفون: Liu W; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou, China 510631., Yu H; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou, China 510631., Zhou X; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou, China 510631., Xing D; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou, China 510631.

المصدر: Analytical chemistry [Anal Chem] 2016 Sep 06; Vol. 88 (17), pp. 8369-74. Date of Electronic Publication: 2016 Aug 10.

نوع المنشور: Letter; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE

مواضيع طبية MeSH: CRISPR-Cas Systems*/genetics , Electrochemical Techniques* , Luminescent Measurements*, DNA/*analysis, DNA/genetics ; Fluorescent Dyes/chemistry ; Molecular Structure

مستخلص: The CRISPR/Cas9 system is a revolutionary genome-editing tool that enables targeted and efficient gene knockouts. However, the off-target effects and loci-dependent enzyme activity limit its uses on the field of research and treatment. In this study, we designed a convenient and sensitive in vitro test method, which was based on electrochemiluminescence (ECL) technology for evaluating cleavage activity of the CRISPR/Cas9 system. It was find that Cas9 can tolerate some common genetic modifications to its target DNA. It was also find that target DNA/sgRNA with single-base mismatch and UV damages of target DNA resulted in significantly reduction of Cas9 cleavage efficiency. Comparing with traditional method, the proposed method reduced the evaluation time from weeks to 2 h. Therefore, our study provides a versatile in vitro method for a priori analysis of CRISPR/Cas9 system and highlights the potential to guide in vivo genome editing.

المؤلفون: Zhao F; Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, Suzhou Research Institute of Southeast University, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu, 210096, China., Chai D, Lu J, Yu J, Liu S

المصدر: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2015 Aug; Vol. 407 (20), pp. 6117-26. Date of Electronic Publication: 2015 Jun 24.

نوع المنشور: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE

مواضيع طبية MeSH: Biomarkers/*blood , Down Syndrome/*diagnosis , High-Throughput Screening Assays/*instrumentation , Luminescent Measurements/*instrumentation , Peroxidase/*chemistry , Glycine max/*enzymology, Antibodies, Immobilized/chemistry ; Biomarkers/analysis ; Chorionic Gonadotropin, beta Subunit, Human/analysis ; Chorionic Gonadotropin, beta Subunit, Human/blood ; Down Syndrome/blood ; Equipment Design ; Estriol/analysis ; Estriol/blood ; Female ; Humans ; Immunoassay/methods ; Inhibins/analysis ; Inhibins/blood ; Pregnancy ; Prenatal Diagnosis/instrumentation ; Sensitivity and Specificity ; alpha-Fetoproteins/analysis

مستخلص: Novel chemiluminescent (CL) imaging microtiter plates with high-throughput, low-cost, and simple operation for detection of four biomarkers related to Down's syndrome screening were developed and evaluated. To enhance the sensitivity of CL immunosensing, soybean peroxidase (SBP) was used instead of horseradish peroxide (HRP) as a label enzyme. The microtiter plates were fabricated by simultaneously immobilizing four capture monoclonal antibodies, anti-inhibin-A, anti-unconjugated oestriol (anti-uE3), anti-alpha-fetoprotein (anti-AFP), and beta anti-HCG (anti-β-HCG), on nitrocellulose (NC) membrane to form immunosensing microtiter wells. Under a sandwiched immunoassay, the CL signals on each sensing site of the microtiter plates were collected by a charge-coupled device (CCD), presenting an array-based chemiluminescence imaging method for detection of four target antigens in a well at the same time. The linear response to the analyte concentration ranged from 0.1 to 40 ng/mL for inhibin-A, 0.075 to 40 ng/mL for uE3, 0.2 to 400 ng/mL for AFP, and 0.4 to 220 ng/mL for β-HCG. The proposed microtiter plates possessed high-throughput, good stability, and acceptable accuracy for detection of four antigens in clinical serum samples and demonstrated potential for practical applicability of the proposed method to Down's syndrome screening. Graphical Abstract Schematic evaluation of the microtiter plater for simultaneous detection of the four biomarkers.

المؤلفون: Liu W; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China., Zhou X; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. Electronic address: zhouxm@scnu.edu.cn., Xing D; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. Electronic address: xingda@scnu.edu.cn.

المصدر: Biosensors & bioelectronics [Biosens Bioelectron] 2014 Aug 15; Vol. 58, pp. 388-94. Date of Electronic Publication: 2014 Mar 12.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE

مواضيع طبية MeSH: Conductometry/*instrumentation , Immunomagnetic Separation/*instrumentation , In Situ Hybridization/*instrumentation , Luminescent Measurements/*instrumentation , MicroRNAs/*analysis , MicroRNAs/*genetics , Microarray Analysis/*instrumentation, Equipment Design ; Equipment Failure Analysis ; Systems Integration

مستخلص: MicroRNAs play pivotal roles in many fundamental aspects of life. Because microRNAs have the characteristics of small size, similar sequence, and low abundance, it is challenging to identify microRNAs rapidly and specifically with high sensitivity. Herein, we developed an electrochemiluminescent (ECL) chip system for microRNA detection based on base-stacking hybridization and magnetic microparticle enrichment technology. In the designed system, the integration of the microfluidic system with ECL detection made it easy to assemble the multiple assay steps and allowed the construction of a device that is convenient to carry. A limit of detection of 1fmol was achieved with this assay. The proposed direct optical microRNA detection technique demonstrated an acceptable sensitivity combined with the advantages of reliability and rapidity.
(Copyright © 2014 Elsevier B.V. All rights reserved.)

المؤلفون: Zhou X; Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, China., Zhu D; Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, China., Liao Y; Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, China., Liu W; Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, China., Liu H; Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, China., Ma Z; Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, China., Xing D; Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, China.

المصدر: Nature protocols [Nat Protoc] 2014 May; Vol. 9 (5), pp. 1146-59. Date of Electronic Publication: 2014 Apr 17.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE

مواضيع طبية MeSH: Electrochemistry/*methods , Luminescent Measurements/*methods , Organometallic Compounds/*chemistry, Antibodies/metabolism ; DNA Probes/metabolism ; Microfluidic Analytical Techniques/methods ; Microspheres ; Propylamines/chemistry

مستخلص: Assays using probes labeled with electrochemiluminescent moieties are extremely powerful analytical tools that are used in fields such as medical diagnostics, environmental analysis and food safety monitoring, in which sensitive, reliable and reproducible detection of biomolecules is a requirement. The most efficient electrochemiluminescence (ECL) reaction to date is based on tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)3(2+)) with tripropylamine (TPrA) as the co-reactant. Here we present a detailed protocol for preparing Ru(bpy)3(2+) probes and their bioanalytical applications. This protocol includes (i) the synthesis of a biologically active Ru(bpy)3(2+)-N-hydroxysuccinimide (NHS) ester, (ii) its covalent labeling with both antibodies and DNA probes and (iii) the detection and quantification of ECL in a microfluidic system with a paramagnetic microbead solid support. In our magnetic bead-based ECL system, two probes are required: a capture probe (labeled with biotin to be captured by a streptavidin-coated magnetic bead) and a detector probe (labeled with Ru(bpy)3(2+)). The complex consisting of the analyte, the capture probe, the detector probe and the magnetic bead is brought into contact with the electrode by using a magnetic field. The Ru(bpy)3(2+) reacts with TPrA in solution to generate the ECL signal. The full protocol, including the synthesis and labeling of the bioactive Ru(bpy)3(2+), requires 5-6 d to complete. ECL immunoassays or nucleic acid tests only require 1.5-2 h, including the sample preparation time.

المؤلفون: Zhang D; Division of Diagnostic Imaging Physics and Webster Center for Advanced Research and Education in Radiation, Department of Radiology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA., Li X, Gao Y, Xu XG, Liu B

المصدر: Medical physics [Med Phys] 2013 Aug; Vol. 40 (8), pp. 081918.

نوع المنشور: Journal Article; Research Support, N.I.H., Extramural

بيانات الدورية: Publisher: John Wiley and Sons, Inc Country of Publication: United States NLM ID: 0425746 Publication Model: Print Cited Medium: Internet ISSN: 2473-4209 (Electronic) Linking ISSN: 00942405 NLM ISO Abbreviation: Med Phys Subsets: MEDLINE

مواضيع طبية MeSH: Luminescent Measurements* , Phantoms, Imaging* , Radiation Dosage*, Radiometry/*instrumentation , Tomography, X-Ray Computed/*instrumentation, Adult ; Calibration ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Monte Carlo Method

مستخلص: Purpose: To present the design and procedure of an experimental method for acquiring densely sampled organ dose map for CT applications, based on optically stimulated luminescence (OSL) dosimeters "nanoDots" and standard ATOM anthropomorphic phantoms; and to provide the results of applying the method--a dose data set with good statistics for the comparison with Monte Carlo simulation result in the future.
Methods: A standard ATOM phantom has densely located holes (in 3×3 cm or 1.5×1.5 cm grids), which are too small (5 mm in diameter) to host many types of dosimeters, including the nanoDots. The authors modified the conventional way in which nanoDots are used, by removing the OSL disks from the holders before inserting them inside a standard ATOM phantom for dose measurements. The authors solved three technical difficulties introduced by this modification: (1) energy dependent dose calibration for raw OSL readings; (2) influence of the brief background exposure of OSL disks to dimmed room light; (3) correct pairing between the dose readings and measurement locations. The authors acquired 100 dose measurements at various positions in the phantom, which was scanned using a clinical chest protocol with both angular and z-axis tube current modulations.
Results: Dose calibration was performed according to the beam qualities inside the phantom as determined from an established Monte Carlo model of the scanner. The influence of the brief exposure to dimmed room light was evaluated and deemed negligible. Pairing between the OSL readings and measurement locations was ensured by the experimental design. The organ doses measured for a routine adult chest scan protocol ranged from 9.4 to 18.8 mGy, depending on the composition, location, and surrounding anatomy of the organs. The dose distribution across different slices of the phantom strongly depended on the z-axis mA modulation. In the same slice, doses to the soft tissues other than the spinal cord demonstrated relatively small variations, with the maximum COV around 11.4%. This might be attributed to the angular mA modulation, the placement of the dosimeters, the chest cavity of the scanned region, and the size of the phantom. Doses to the spinal cord were consistently lower than those to other soft tissues.
Conclusions: The method is suited for acquiring densely sampled organ dose maps, and can be used for studying dose distributions relevant to subject size, organ location, and clinical CT protocols.

المؤلفون: Zhan F; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China., Zhou X, Xing D

المصدر: Analytica chimica acta [Anal Chim Acta] 2013 Jan 25; Vol. 761, pp. 71-7. Date of Electronic Publication: 2012 Nov 23.

نوع المنشور: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4324 (Electronic) Linking ISSN: 00032670 NLM ISO Abbreviation: Anal Chim Acta Subsets: MEDLINE

مواضيع طبية MeSH: Luminescent Measurements/*methods , Reverse Transcriptase Polymerase Chain Reaction/*methods , Rotavirus/*genetics , Rotavirus/*isolation & purification , Rotavirus Infections/*diagnosis , Rotavirus Infections/*virology, DNA Primers/genetics ; DNA, Complementary/genetics ; Humans ; Luminescent Measurements/economics ; Magnets/chemistry ; RNA, Viral/genetics ; RNA, Viral/isolation & purification ; Reverse Transcriptase Polymerase Chain Reaction/economics ; Reverse Transcription ; Sensitivity and Specificity ; Time Factors

مستخلص: A novel method for detection of rotavirus has been developed by integrating magnetic primer based reverse transcription-polymerase chain reaction (RT-PCR) with electrochemiluminescence (ECL) detection. This is realized by accomplishing RT of rotavirus RNA in traditional way and performing PCR of the resulting cDNA fragment on the surface of magnetic particles (MPs). In order to implement PCR on MPs and achieve rapid ECL detection, forward and reverse primers are bounded to MPs and tris-(2,2'-bipyridyl) ruthenium (TBR), respectively. After RT-PCR amplification, the TBR labels are directly enriched onto the surface of MPs. Then the MPs-TBR complexes can be loaded on the electrode surface and analyzed by magnetic ECL platform without any post-modification or post-incubation process. So some laborious manual operations can be avoided to achieve rapid yet sensitive detection. In this study, rotavirus in fecal specimens was successfully detected within 1.5 h. Experimental results showed that the detection limit of the assay was 0.2 pg μL(-1) of rotavirus. The ECL intensity was linearly with the concentration from 0.2 pg μL(-1) to 400 pg μL(-1). What's more, the specificity of this method was confirmed by detecting other fecal specimens of patients with nonrotavirus-associated gastroenteritis. We anticipate that the proposed magnetic primer based RT-PCR with ECL detection strategy will find numerous applications in food safety field and clinical diagnosis.
(Copyright © 2012 Elsevier B.V. All rights reserved.)