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المؤلفون: Li, Deqiang, Li, Ming, Liu, Peilai, Zhang, Yuankai, Lu, Jianxi, Li, Jianmin

المصدر: International Orthopaedics; Nov2014, Vol. 38 Issue 11, p2399-2406, 8p

مصطلحات موضوعية: TISSUE engineering, BONE surgery, BIOREACTORS, BONE injuries, SHEEP diseases, MESENCHYMAL stem cells, THERAPEUTICS

مستخلص: Purpose: Repair of bone defects, particularly critical-sized bone defects, is a considerable challenge in orthopaedics. Tissue-engineered bones provide an effective approach. However, previous studies mainly focused on the repair of bone defects in small animals. For better clinical application, repairing critical-sized bone defects in large animals must be studied. This study investigated the effect of a tissue-engineered bone for repairing critical-sized bone defect in sheep. Methods: A tissue-engineered bone was constructed by culturing bone marrow mesenchymal-stem-cell-derived osteoblast cells seeded in a porous β-tricalcium phosphate ceramic (β-TCP) scaffold in a perfusion bioreactor. A critical-sized bone defect in sheep was repaired with the tissue-engineered bone. At the eighth and 16th week after the implantation of the tissue-engineered bone, X-ray examination and histological analysis were performed to evaluate the defect. The bone defect with only the β-TCP scaffold served as the control. Result: X-ray showed that the bone defect was successfully repaired 16 weeks after implantation of the tissue-engineered bone; histological sections showed that a sufficient volume of new bones formed in β-TCP 16 weeks after implantation. Eight and 16 weeks after implantation, the volume of new bones that formed in the tissue-engineered bone group was more than that in the β-TCP scaffold group ( P < 0.05). Conclusion: Tissue-engineered bone improved osteogenesis in vivo and enhanced the ability to repair critical-sized bone defects in large animals. [ABSTRACT FROM AUTHOR]

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