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المؤلفون: Ryota Yamano, Juanwen Yu, Chunqi Jiang, Alfabetian Harjuno Condro Haditomo, Sayaka Mino, Yuichi Sakai, Tomoo Sawabe
المصدر: PLOS ONE. 17:e0271174
مصطلحات موضوعية: DNA, Bacterial, Oceanospirillaceae, Base Composition, Multidisciplinary, RNA, Ribosomal, 16S, Fatty Acids, Nucleic Acid Hybridization, Genomics, Sequence Analysis, DNA, Phospholipids, Phylogeny, Bacterial Typing Techniques
الوصف: A Gram-staining-negative, aerobic bacterium, designated strain PT3T was isolated from laboratory-reared larvae of the Japanese sea cucumber Apostichopus japonicus. Phylogenetic analysis based on the 16S rRNA gene nucleotide sequences revealed that PT3T was closely related to Amphritea ceti RA1T (= KCTC 42154T = NBRC 110551T) and Amphritea spongicola MEBiC05461T (= KCCM 42943T = JCM 16668T) both with 98.3% sequence similarity, however, average nucleotide identity (ANI) and in silico DNA-DNA hybridization (in silico DDH) values among these three strains were below 95% and 70%, respectively, confirming the novelty of PT3T. Furthermore, the average amino acid identity (AAI) values of PT3T against other Amphritea species were on the reported genus delineation boundary (64–67%). Multilocus sequence analysis using four protein-coding genes (recA, mreB, rpoA, and topA) further demonstrated that PT3T, Amphritea ceti and Amphritea spongicola formed a monophyletic clade clearly separate from other members of the genus Amphritea. Three strains (PT3T, A. ceti KCTC 42154T and A. spongicola JCM 16668T) also showed higher similarities in their core genomes compared to those of the other Amphritea spp. Based on the genome-based taxonomic approach, Aliamphritea gen. nov. was proposed together with the reclassification of the genus Amphritea and Aliamphritea ceti comb. nov. (type strain RA1T = KCTC 42154T = NBRC 110551T), Aliamphritea spongicola comb. nov. (type strain MEBiC05461T = KCCM 42943T = JCM 16668T), and Aliamphritea hakodatensis sp. nov. (type strain PT3T = JCM 34607T = KCTC 82591T) were suggested.
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المؤلفون: Gunjan Sharma, Suresh Thakur, Chandni Sidhu, Anil Kumar Pinnaka, Naga Radha Srinivas Tanuku
المصدر: International Journal of Systematic and Evolutionary Microbiology. 67:3428-3434
مصطلحات موضوعية: DNA, Bacterial, Oceanospirillaceae, 0106 biological sciences, 0301 basic medicine, Geologic Sediments, Linum, food.ingredient, India, Biology, 010603 evolutionary biology, 01 natural sciences, Microbiology, 03 medical and health sciences, food, Phylogenetics, RNA, Ribosomal, 16S, Botany, Agar, Phospholipids, Phylogeny, Ecology, Evolution, Behavior and Systematics, Base Composition, Phylogenetic tree, Strain (chemistry), DNA–DNA hybridization, Fatty Acids, Nucleic Acid Hybridization, Sediment, Sequence Analysis, DNA, General Medicine, biology.organism_classification, 16S ribosomal RNA, Bacterial Typing Techniques, 030104 developmental biology
الوصف: A novel Gram-staining-negative, spiral-shaped, pale-yellow, non-sporulating, motile, aerobic bacterium, designated strain AK56T, was isolated from a sediment sample collected at the Coringa Wildlife Sanctuary, India. Colonies on marine agar were circular, pale yellow, shiny, translucent, 1-2 mm in diameter, convex and had an entire margin. The major fatty acids included C16 : 1, C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, one unidentified phospholipid and five unidentified lipids. DNA-DNA hybridization between strain AK56T and Oceanospirillum linum LMG 5214T and 'Oceanospirillum nioense ' NIO-S6 showed relatedness values of 39.91 and 23.62 %, respectively. The DNA G+C content of strain AK56T was found to be 50.3 mol%. A sequence similarity search for the 16S rRNA gene sequence revealed that O. linum and O. nioense were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 98.9 and 98.2 %, respectively. Phylogenetic analysis also showed the formation of a cluster including strain AK56T with close relative O. linum and O. nioense. Based on the observed phenotypic, chemotaxonomic characteristics and phylogenetic analysis, strain AK56T is described in this study as a novel species in the genus Oceanospirillum, for which the name Oceanospirillum sanctuarii sp. nov. is proposed. The type strain of Oceanospirillumsanctuarii is AK56T (=MTCC 12005T=JCM 19193T=KCTC 52973T).
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d26f1d4c9ce7d08d0f9e5bc6f0c9999
https://doi.org/10.1099/ijsem.0.002132 -
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المؤلفون: Wei Fang, Yazhong Xiao, Jing Zhang, Zemin Fang, Xiaoyan Han, Juanjuan Liu, Yanfeng Zhang
المصدر: International journal of systematic and evolutionary microbiology. 69(6)
مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, DNA, Bacterial, Oceanospirillaceae, Geologic Sediments, Ubiquinone, Antarctic Regions, 010603 evolutionary biology, 01 natural sciences, Microbiology, Japonica, 03 medical and health sciences, Genus, RNA, Ribosomal, 16S, Botany, Ecology, Evolution, Behavior and Systematics, Phospholipids, Phylogeny, Base Composition, biology, Phylogenetic tree, Strain (chemistry), Fatty Acids, Nucleic Acid Hybridization, General Medicine, Sequence Analysis, DNA, biology.organism_classification, 16S ribosomal RNA, Terpenoid, Bacterial Typing Techniques, 030104 developmental biology, Bacteria
الوصف: A taxonomic study was carried out on a Gram-stain-negative bacterium, namely strain ANRC-JH13T, isolated from a sediment sample collected at Jasper beach, adjacent to Fildes Peninsula, Antarctica. Cells of strain ANRC-JH13T were non-spore-forming rods and motile by the way of flagellum. Strain ANRC-JH13T was facultatively anaerobic, oxidase-positive, and catalase-positive. Growth of strain ANRC-JH13T occurred at 10–42 °C (optimum, 28 °C), pH 4.0–11.0 (pH 7.0) and 0–12.0 % (w/v) NaCl (1.0–2.0 %). Its predominant fatty acids were C16 : 0 (21.7 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 38.3 %), and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 20.1 %). Isoprenoid quinone Q-8 was the major respiratory quinone. Its major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, and four unknown polar lipids. The DNA G+C content was 48 mol%. Strain ANRC-JH13T showed the highest 16S rRNA gene sequence similarity to Amphritea balenae JAMM 1525T (97.9 %), followed by Amphritea atlantica M41T (97.8 %) and Amphritea japonica JAMM 1866T (97.3 %), and formed a lineage within the genus Amphritea on the phylogenetic trees. However, the in silico average nucleotide identity values between strain ANRC-JH13T and A. balenae JAMM 1525T, A. atlantica M41T, and A. japonica JAMM 1866T were 74.0, 76.7, and 74.9 %, respectively. The in silico DNA–DNA hybridization values between them were 19.8, 20.6, and 19.4 %, respectively. Based on the results from phenotypic, chemotaxonomic, and phylogenetic analyses, strain ANRC-JH13T is considered to represent a novel species of the genus Amphritea , for which the name Amphritea opalescens sp. nov. is proposed. The type strain is ANRC-JH13T (=MCCC 1K03512T=KCTC 62532T).
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d517826c2d167d57bebb744fd982c9b2
https://pubmed.ncbi.nlm.nih.gov/30912740 -
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المؤلفون: Yangzom Pema, Dorji Phurbu, Peng Xing, Yanling Tian, Huabing Li, Huibin Lu, Chao Ma
المصدر: International journal of systematic and evolutionary microbiology. 69(1)
مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, DNA, Bacterial, Oceanospirillaceae, Ubiquinone, Biology, Tibet, 010603 evolutionary biology, 01 natural sciences, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Genus, RNA, Ribosomal, 16S, Botany, Ecology, Evolution, Behavior and Systematics, Phospholipids, Phylogeny, Phosphatidylglycerol, geography, Base Composition, Plateau, geography.geographical_feature_category, Phylogenetic tree, Strain (chemistry), Fatty Acids, Nucleic Acid Hybridization, General Medicine, Sequence Analysis, DNA, 16S ribosomal RNA, biology.organism_classification, Bacterial Typing Techniques, genomic DNA, Lakes, 030104 developmental biology, chemistry, lipids (amino acids, peptides, and proteins), Bacteria
الوصف: A novel Gram-stain-negative, motile and rod-shaped bacterium, designated xg18T, was isolated from Lake XuguoCo on the Tibetan Plateau. The strain was able to grow optimally at 0–2 % NaCl and tolerate up to 6 % NaCl. Growth occurred at pH 7.0–11.0 (optimum, pH 9.0–10.0) and 15–40 °C (optimum, 37 °C). Vitamins were not required for growth. The major polar lipids of strain xg18T were phosphatidyl ethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. The major fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The G+C content of genomic DNA was 46.1 mol%. Analysis of 16S rRNA sequences showed that strain xg18T belongs to the genus Nitrincola , with Nitrincola alkalisediminis MEB087T (KC822363, 98.6 %) as its closest neighbour. The DNA–DNA relatedness value of strain xg18T with its closest phylogenetic neighbour, N. alkalisediminis JCM 19317T, was 43.1±3.2 %. Strain xg18T was clearly distinguishable from the type strain of the genus Nitrincola through phylogenetic analysis, fatty acid composition data and a range of physiological and biochemical characteristics comparisons. Based on its phenotypic and chemotaxonomic characteristics, strain xg18T could be classified as a representative of a novel species of the genus for which the name Nitrincola tibetensis sp. nov. is proposed. The type strain is xg18T (=CICC 24457T=KCTC 62401T).
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d69ecd065783c93d342b55ac0c603f3
https://pubmed.ncbi.nlm.nih.gov/30431420 -
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المؤلفون: Shih-Yi Sheu, Pei-Bei Xie, Wen-Ming Chen, Der-Shyan Sheu, Sen-Lin Tang
المصدر: International journal of systematic and evolutionary microbiology. 68(1)
مصطلحات موضوعية: 0301 basic medicine, DNA, Bacterial, Oceanospirillaceae, Biology, Microbiology, 03 medical and health sciences, RNA, Ribosomal, 16S, Botany, Animals, Gene, Ecology, Evolution, Behavior and Systematics, Phospholipids, Phylogeny, Base Composition, Phylogenetic tree, Fatty Acids, Nucleic Acid Hybridization, Vitamin K 2, General Medicine, Sequence Analysis, DNA, biology.organism_classification, 16S ribosomal RNA, Anthozoa, Terpenoid, Bacterial Typing Techniques, genomic DNA, 030104 developmental biology, Taxonomy (biology), Proteobacteria
الوصف: Strain Eup a-2T, isolated from the torch coral Euphyllia glabrescens, was characterized using a polyphasic taxonomy approach. Cells of strain Eup a-2T were Gram-negative, aerobic and motile by three polar flagella and formed translucent colonies. Optimal growth occurred at 25 °C, pH 6–8 and in the presence of 2–4 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Eup a-2T belonged to the genus Litoribrevibacter and showed the highest levels of sequence similarity with respect to Litoribrevibacter albus Y32T (97.8 %). Strain Eup a-2T contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 as the predominant fatty acids. The predominant isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphophatidylglycerol. Genomic DNA G+C content of strain Eup a-2T was 49.1 mol%. The DNA–DNA hybridization value for strain Eup a-2T with L. albus Y32T was less than 30 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain Eup a-2T should be classified as a novel species of the genus Litoribrevibacter , for which the name Litoribrevibacter euphylliae sp. nov. is presented. The type strain is Eup a-2T (=BCRC 81004T=LMG 29725T=KCTC 52438T).
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df5911b9b7ff5b86f02682cb4572f33f
https://pubmed.ncbi.nlm.nih.gov/29235976 -
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المؤلفون: João Serôdio, Lígia Sousa, Jörg C. Frommlet, Artur Alves, Bárbara Guimarães
المصدر: International Journal of Systematic and Evolutionary Microbiology. 65:915-919
مصطلحات موضوعية: DNA, Bacterial, Oceanospirillaceae, Ubiquinone, Molecular Sequence Data, Biology, Sea anemone, Microbiology, Symbiodinium, Tagetes, RNA, Ribosomal, 16S, Botany, Animals, Symbiosis, Phylogeny, Ecology, Evolution, Behavior and Systematics, Base Composition, Strain (chemistry), Fatty Acids, Puerto Rico, Dinoflagellate, Nucleic Acid Hybridization, Sequence Analysis, DNA, General Medicine, biology.organism_classification, 16S ribosomal RNA, Bacterial Typing Techniques, Sea Anemones, Dinoflagellida, Aiptasia, Bacteria
الوصف: A Gram-stain-negative, facultatively anaerobic, oxidase- and catalase-positive, rod-shaped bacterium, strain SYM1T, was isolated from a culture of Symbiodinium sp., an algal symbiont of the sea anemone Aiptasia tagetes collected in Puerto Rico. Growth was observed at 4–40 °C (optimum 30 °C), at pH 5.0–11.0 (optimum pH 8.0) and with 0.5–8 % (optimum 2 %) (w/v) NaCl. Phylogenetic analyses of 16S rRNA gene sequences showed that strain SYM1T was a member of the genus Neptunomonas with the type strain of Neptunomonas naphthovorans as the closest phylogenetic relative with a pairwise sequence similarity of 98.15 %. However, DNA–DNA relatedness between strain SYM1T and N. naphthovorans CIP 106451T was 24 %. Moreover, strain SYM1T could be distinguished from its closest relative by several phenotypic characteristics such as NaCl, pH and temperature tolerance, nitrate reduction and utilization of carbon substrates. The major cellular fatty acids were C16 : 0, C18 : 1ω7c and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH). The genomic DNA G+C content of strain SYM1T was 45 mol%. Ubiquinone-8 (Q-8) was the only respiratory quinone detected. Based on a polyphasic taxonomic characterization, strain SYM1T represents a novel species of the genus Neptunomonas , for which the name Neptunomonas phycophila sp. nov. is proposed. The type strain is SYM1T ( = LMG 28329T = CECT 8716T).
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1827def0bc84a0710182d31807b977d6
https://doi.org/10.1099/ijs.0.000039 -
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المؤلفون: Ana L. Diéguez, Sabela Balboa, Jesús L. Romalde, Thorolf Magnesen
المصدر: Systematic and applied microbiology. 40(2)
مصطلحات موضوعية: 0301 basic medicine, DNA, Bacterial, Oceanospirillaceae, Zoology, Biology, Applied Microbiology and Biotechnology, Microbiology, DNA, Ribosomal, 03 medical and health sciences, Genus, RNA, Ribosomal, 16S, Pecten maximus, Animals, Cluster Analysis, Ecology, Evolution, Behavior and Systematics, Phospholipids, Phylogeny, Pecten, Phylogenetic tree, Ecology, Norway, Nucleic Acid Hybridization, DNA-Directed RNA Polymerases, Sequence Analysis, DNA, Ribosomal RNA, 16S ribosomal RNA, rpoB, biology.organism_classification, Hatchery, Aerobiosis, Bacterial Typing Techniques, 030104 developmental biology, Scallop, Locomotion
الوصف: Nine isolates obtained from a great scallop hatchery in Norway were characterized using a polyphasic approach. Strains were Gram-negative, aerobic and motile rods with oxidative metabolism. Phylogenetic analysis based on the sequences of 16S rRNA and rpoB genes showed that these strains formed two different groups associated with members of the genus Neptuniibacter. DNA–DNA hybridization (DDH) and Average Nucleotide Identity (ANI) demonstrated that the isolates constituted two novel species of this genus, which can be phenotypically differentiated from their closest relatives. The names Neptuniibacter marinus sp. nov. and Neptuniibacter pectenicola sp. nov are proposed, with ATR 1.1T (=CECT 8938T = DSM 100783T) and LFT 1.8T (=CECT 8936T = DSM 100781T) as respective type strains.
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المؤلفون: Yu-Zhong Zhang, Chun-Xiao Chen, Zhong Xu, Bin-Bin Xie, Ang Liu, Qi-Long Qin, Xiu-Lan Chen, Xi-Ying Zhang, Guo-Wei Li, Hai Li, Bai-Cheng Zhou, Chang Liu
المصدر: International Journal of Systematic and Evolutionary Microbiology. 63:1673-1677
مصطلحات موضوعية: DNA, Bacterial, Oceanospirillaceae, China, Sequence analysis, Molecular Sequence Data, Biology, Microbiology, Japonica, Phylogenetics, RNA, Ribosomal, 16S, Botany, Seawater, Phylogeny, Ecology, Evolution, Behavior and Systematics, Base Composition, Oxidase test, Phylogenetic tree, Phosphatidylethanolamines, Fatty Acids, Quinones, Nucleic Acid Hybridization, Phosphatidylglycerols, Sequence Analysis, DNA, General Medicine, Ribosomal RNA, Silicon Dioxide, 16S ribosomal RNA, biology.organism_classification, Bacterial Typing Techniques, genomic DNA
الوصف: A Gram-negative, motile, aerobic, oxidase- and catalase-positive rod, designated P10-2-4T, was isolated from an intertidal sand sample collected from a coastal area of Qingdao (Yellow Sea), China. The isolate reduced nitrate to nitrite and grew at 4–33 °C and with 0.5–12 % (w/v) NaCl. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C18 : 1ω7c and C16 : 0. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major respiratory quinone was Q-8. The genomic DNA G+C content was 45.1 %. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain P10-2-4T belonged to the genus Neptunomonas . The isolate shared the highest 16S rRNA gene sequence similarity (98.1 %) with Neptunomonas japonica JAMM 0745T and 96.9, 96.5 and 95.9 % sequence similarities with N. antarctica S3-22T, N. concharum LHW37T and N. naphthovorans NAG-2N-126T, respectively, strains of the other three recognized species in the genus. DNA–DNA relatedness between strain P10-2-4T and N. japonica JCM 14595T was 35.6 %. Furthermore, strain P10-2-4T could be distinguished from the representatives of the genus Neptunomonas by a combination of phenotypic characteristics, such as temperature and NaCl concentration for growth, nitrate reduction, DNase activity and assimilation of substrates. The data from this study suggests that strain P10-2-4T represents a novel species in the genus Neptunomonas , for which the name Neptunomonas qingdaonensis sp. nov. is proposed. The type strain is P10-2-4T ( = CGMCC 1.10971T = KCTC 23686T).
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ba0606e17d18a758a3be97cfcaae314
https://doi.org/10.1099/ijs.0.041970-0 -
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المؤلفون: Na-Ri Shin, Tae Woong Whon, Hae-Won Lee, Jina Lee, Jin-Woo Bae, Seong Woon Roh
المصدر: International Journal of Systematic and Evolutionary Microbiology. 62:2657-2661
مصطلحات موضوعية: DNA, Bacterial, Oceanospirillaceae, Ubiquinone, Molecular Sequence Data, Neptunomonas concharum, Ark clam, Microbiology, RNA, Ribosomal, 16S, Republic of Korea, Botany, Genus Neptunomonas, Animals, Phospholipids, Phylogeny, Ecology, Evolution, Behavior and Systematics, Base Composition, Phylogenetic tree, biology, Strain (chemistry), Fatty Acids, Nucleic Acid Hybridization, Sequence Analysis, DNA, General Medicine, biology.organism_classification, 16S ribosomal RNA, Bacterial Typing Techniques, Bivalvia, genomic DNA, Bacteria
الوصف: A novel Gram-staining-negative, facultatively anaerobic, motile and rod-shaped bacterium, designated strain LHW37T, was isolated from a dead ark clam collected on the south coast of Korea. The novel strain grew optimally at 37 °C, at pH 7.0–8.0 and with 2 % (w/v) NaCl. The predominant cellular fatty acids were C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The major isoprenoid quinone was ubiquinone-8 (Q-8) and the predominant polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain was most closely related to Neptunomonas japonica JAMM 0745T (97.1 % sequence similarity). The genomic DNA G+C content of strain LHW37T was 48.2 mol%. The DNA–DNA relatedness values recorded in hybridization experiments between the novel strain and its closest known relative were ≤18 %. Based on the phenotypic, genotypic and phylogenetic data, strain LHW37T represents a novel species belonging to the genus Neptunomonas for which the name Neptunomonas concharum sp. nov. is proposed. The type strain is LHW37T ( = KACC 15543T = JCM 17730T). An emended description of the genus Neptunomonas is also provided.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d1f52f38c2d71ae41c43d3f3a0470d8
https://doi.org/10.1099/ijs.0.037473-0 -
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المؤلفون: Ana L. Diéguez, Phillip Pichon, Aide Lasa, Jesús L. Romalde
المصدر: International journal of systematic and evolutionary microbiology. 66(8)
مصطلحات موضوعية: 0301 basic medicine, DNA, Bacterial, food.ingredient, Aquaculture, Microbiology, 03 medical and health sciences, food, Marinomonas atlantica, RNA, Ribosomal, 16S, Animals, Marinomonas, Ecology, Evolution, Behavior and Systematics, Phylogeny, Base Composition, biology, Marinomonas gallaica, Phylogenetic tree, Ecology, Aquimarina, Strain (biology), Fatty Acids, Nucleic Acid Hybridization, General Medicine, Sequence Analysis, DNA, 16S ribosomal RNA, biology.organism_classification, Bacterial Typing Techniques, Bivalvia, genomic DNA, 030104 developmental biology, Spain, Oceanospirillaceae
الوصف: Three Gram-negative bacterial strains (Cmf 17.2T, Rd 20.33 and Cmf 18.22T) isolated from reared clams in Galicia were subjected to a taxonomic study, based on genetic and phenotypic characterization. Analysis of the 16S rRNA gene allowed the identification of the strains as members of the genus Marinomonas, sharing the highest similarity with Marinomonas aquimarina CECT 5080T (97.8 %-98.5 % 16S rRNA gene sequence similarity). Phylogenetic analysis of the sequences showed that the three isolates formed two different groups distantly related to their closest relative, M. aquimarina. DNA-DNA hybridizations were performed to confirm the taxonomic position and the results were below the recommended threshold for species delimitation, specifically 44.5 % (Cmf 17.2T with M. aquimarina CECT 5080T) and 55 % (Cmf 18.22Twith M. aquimarina CECT 5080T). Furthermore, the average nucleotide identity (ANIb, ANIm and OrthoANI) and in silico estimated DNA-DNA reassociation values among Cmf 17.2T, Cmf 18.22T and M. aquimarina CECT 5080T were in all cases below the respective threshold for species differentiation. The estimated G+C content of the genomic DNA was found to be 45.3 % (Cmf 17.2T) and 44.6 % (Cmf 18.22T). The principal fatty acids of the strains were found to be summed feature 3 (C16 : 1 ω7c/C16 : 1ω6c), summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), C16 : 0, C12 : 0 and C10 : 0 3-OH. The results obtained on the characterization of the clam isolates indicate that they represent two novel species of the genus Marinomonas, for which the names Marinomonas gallaica sp. nov. (type strain Cmf 17.2T=CECT 9049T=LMG 29243T) and Marinomonas atlantica sp. nov. (type strain Cmf 18.22T=CECT 9050T=LMG 29244T) are proposed.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29b9271cc142746d3fda63b974736ee2
https://pubmed.ncbi.nlm.nih.gov/27199280
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