المؤلفون: Qi K; Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic Materials, School of Materials Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.; Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.; Department of Biomedical Engineering, Fourth Military Medical University, Xi'an 710032, China., Lu Z; College of Life Sciences, Northwest University, Xi'an 710069, China., Gao X; Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China., Tan G; Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic Materials, School of Materials Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China., Zhang Z; College of Life Sciences, Northwest University, Xi'an 710069, China., Liu D; College of Life Sciences, Northwest University, Xi'an 710069, China., Dong G; Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic Materials, School of Materials Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China., Jing D; Department of Biomedical Engineering, Fourth Military Medical University, Xi'an 710032, China., Luo P; Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.

المصدر: ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2024 Jun 12; Vol. 16 (23), pp. 29793-29804. Date of Electronic Publication: 2024 May 31.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE

مواضيع طبية MeSH: Glioma*/drug therapy , Glioma*/pathology , Glioma*/metabolism , Photochemotherapy* , Photosensitizing Agents*/chemistry , Photosensitizing Agents*/pharmacology , Photosensitizing Agents*/therapeutic use , Brain Neoplasms*/drug therapy , Brain Neoplasms*/pathology , Brain Neoplasms*/metabolism , Nitriles*/chemistry , Nitriles*/pharmacology, Animals ; Mice ; Cell Line, Tumor ; Humans ; Reactive Oxygen Species/metabolism

مستخلص: The effectiveness of photodynamic therapy (PDT) in treating brain gliomas is limited by the solubility of photosensitizers and the production of reactive oxygen species (ROS), both of which are influenced by the concentration of photosensitizers and catalyst active sites. In this study, we developed a controllable surface hydroxyl concentration for the photosensitizer CN11 to address its poor water solubility issue and enhance PDT efficacy in tumor treatment. Compared to pure g-C 3 N 4 (CN), CN11 exhibited 4.6 times higher hydrogen peroxide production under visible light, increased incidence of the n → π* electron transition, and provided more available reaction sites for cytotoxic ROS generation. These findings resulted in a 2.43-fold increase in photodynamic treatment efficacy against brain glioma cells. Furthermore, in vivo experiments conducted on mice demonstrated that CN11 could be excreted through normal cell metabolism with low cytotoxicity and high biosafety, effectively achieving complete eradication of tumor cells.

المؤلفون: Zhao Z; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China., Yang J; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China., Liu Y; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China., Wang S; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China.; School of Pharmaceutical Engineering & Institute for Advanced Studies, Taizhou University, 1139 Shifu Road, Taizhou, Zhejiang 318000, China. dama@fudan.edu.cn., Zhou W; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China., Li ZT; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China., Zhang DW; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China., Ma D; School of Pharmaceutical Engineering & Institute for Advanced Studies, Taizhou University, 1139 Shifu Road, Taizhou, Zhejiang 318000, China. dama@fudan.edu.cn.

المصدر: Journal of materials chemistry. B [J Mater Chem B] 2023 Sep 27; Vol. 11 (37), pp. 9027-9034. Date of Electronic Publication: 2023 Sep 27.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101598493 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-7518 (Electronic) Linking ISSN: 2050750X NLM ISO Abbreviation: J Mater Chem B Subsets: MEDLINE

مواضيع طبية MeSH: Photochemotherapy*, Animals ; Humans ; Mesoporphyrins ; HeLa Cells

مستخلص: Acyclic cucurbit[ n ]uril-based nanosponges are prepared based on supramolecular vesicle-templated cross-linking. The nanosponges are capable of encapsulating the clinically approved photodynamic therapeutic (PDT) drug temoporfin. When loaded with nanosponges, the PDT bioactivity of temoporfin is enhanced 7.5-fold for HeLa cancer cells and 20.8 fold for B16-F10 cancer cells, respectively. The reason for the significant improvement in PDT efficacy is confirmed to be an enhanced cell uptake by confocal laser scanning microscopy and flow cytometry. Animal studies show that nanosponges could dramatically increase the tumor suppression effect of temoporfin. In vitro and in vivo experiments demonstrate that nanosponges are nontoxic and biocompatible.

المؤلفون: Zeng Q; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University Guangzhou, 510631, PR China., Li X; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University Guangzhou, 510631, PR China., Xie S; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University Guangzhou, 510631, PR China., Xing D; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University Guangzhou, 510631, PR China. Electronic address: xingda@scnu.edu.cn., Zhang T; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University Guangzhou, 510631, PR China; Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou, 510631, China. Electronic address: zt@scnu.edu.cn.

المصدر: Biomaterials [Biomaterials] 2022 Nov; Vol. 290, pp. 121867. Date of Electronic Publication: 2022 Oct 22.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE

مواضيع طبية MeSH: Prodrugs*/chemistry , Neoplasms*/drug therapy , Photochemotherapy* , Nanoparticles*/chemistry, Humans ; Photosensitizing Agents/therapeutic use ; Photosensitizing Agents/chemistry ; Cisplatin/therapeutic use ; Glutathione ; Cell Line, Tumor

مستخلص: Developing single molecule-assembled nanoprodrugs that can reverse the glutathione (GSH)-mediated drug resistance of tumors provides promising potentials for precision and effective cancer theranostics. Herein, we developed a novel single molecule-assembled nanoprodrug for effective photodynamic/chemotherapeutic eradication of drug-resistant tumors via the multistage GSH-depletion. The nanoprodrug MSSP-NP could be fancily fabricated by self-assembly of an amphiphilic activatable molecular MSSP, which is synthesized by covalently conjugating the photosensitizer methylene blue (MB) with a GSH-sensitive cisplatin prodrug via a tumor targeting thiolated polypeptide. Upon the nanoprodrug MSSP-NP systematic administrated, its photoactivities and pharmacological effects can be thoroughly switched on by intracellular GSH to produce the photosensitizer MB and chemotherapeutic drug cisplatin, along with the multi-step consumption of GSH, which could remarkably boost oxidative stress and reverse the drug resistance. As a result, the nanoprodrug could effectively disrupt the tumor GSH-defense system to afford high-efficiency photodynamic/chemotherapeutic inhibition of drug resistance tumors (96.4%) with minimum side effects.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)

المؤلفون: Liu Y; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China., Wang ZK; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China., Gao ZZ; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China., Zong Y; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China., Sun JD; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China., Zhou W; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China. Electronic address: zhouw@fudan.edu.cn., Wang H; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China., Ma D; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China., Li ZT; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China. Electronic address: ztli@fudan.edu.cn., Zhang DW; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China. Electronic address: zhangdw@fudan.edu.cn.

المصدر: Acta biomaterialia [Acta Biomater] 2022 Sep 15; Vol. 150, pp. 254-264. Date of Electronic Publication: 2022 Jul 30.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 101233144 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-7568 (Electronic) Linking ISSN: 17427061 NLM ISO Abbreviation: Acta Biomater Subsets: MEDLINE

مواضيع طبية MeSH: Photochemotherapy*/methods , Porphyrins*/pharmacology, Animals ; Cell Line, Tumor ; Mice ; Photosensitizing Agents/chemistry ; Polymers ; Porosity

مستخلص: Since 1995, photodynamic therapy (PDT) has been utilized as an effective method for cancer treatment. However, the residues of photosensitizers in the normal tissues after PDT can be activated by sunlight to cause severe skin phototoxicity, for which currently there are no clinical solutions. As a result, post-PDT patients need to remain out of sunlight for up to five weeks, which produces great living and mental burdens for patients. Herein, we report that a biocompatible porous organic polymer (POP) with average 3.1 nm porosity is able to suppress the skin phototoxicity of clinically used porphyrin-based photodynamic agents (PDAs), including Photofrin, Talaporfin and Hiporfin, through an adsorption-elimination mechanism. Fluorescence titration and dialysis experiments show that POP can adsorb and retain the PDAs at a micromolar concentration. In vivo experiments demonstrate that POP can significantly suppress the skin phototoxicity caused by all the three PDAs without reducing their PDT efficacy. STATEMENT OF SIGNIFICANCE: Up to now, no efficient clinical treatment for the inhibition of post-PDT phototoxicity of clinically used porphyrin-based PDAs is available. In the manuscript, a water-soluble cationic porous organic polymer has been revealed to include three clinically used PDAs. In vivo experiments show that this inclusion remarkably reduces the content of PDAs in mouse skins, leading to significant alleviation of their post-PDT phototoxicity without no negative effect on their PDT efficacy. Thus, this work provides a strategy for overcoming the drawback of clinically used photodynamic agents.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022. Published by Elsevier Ltd.)

المؤلفون: Yang Y; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China., Huang J; State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, 510641, China., Wei W; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China., Zeng Q; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China., Li X; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China., Xing D; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China., Zhou B; State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, 510641, China. zhoubo@scut.edu.cn., Zhang T; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China. zt@scnu.edu.cn.; Guangzhou Key Laboratory of Spectral Analysis & Functional Probes, College of Biophotonics, South China Normal University, Guangzhou, 510631, China. zt@scnu.edu.cn.

المصدر: Nature communications [Nat Commun] 2022 Jun 07; Vol. 13 (1), pp. 3149. Date of Electronic Publication: 2022 Jun 07.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE

مواضيع طبية MeSH: Nanoparticles* , Photoacoustic Techniques* , Photochemotherapy*, Photosensitizing Agents/therapeutic use ; Phototherapy

مستخلص: Phototheranostics based on upconversion nanoparticles (UCNPs) offer the integration of imaging diagnostics and phototherapeutics. However, the programmable control of the photoactivation of imaging and therapy with minimum side effects is challenging due to the lack of ideal switchable UCNPs agents. Here we demonstrate a facile strategy to switch the near infrared emission at 800 nm from rationally designed UCNPs by modulating the irradiation laser into pulse output. We further synthesize a theranostic nanoagent by combining with a photosensitizer and a photoabsorbing agent assembled on the UCNPs. The orthogonal activation of in vivo photoacoustic imaging and photodynamic therapy can be achieved by altering the excitation modes from pulse to continuous-wave output upon a single 980 nm laser. No obvious harmful effects during photoexcitation was identified, suggesting their use for long-term imaging-guidance and phototherapy. This work provides an approach to the orthogonal activation of imaging diagnostics and photodynamic therapeutics.
(© 2022. The Author(s).)

المؤلفون: Liu Y; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China., Liu CZ; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China., Wang ZK; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China., Zhou W; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China., Wang H; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China., Zhang YC; Department of Inorganic Chemistry, School of Pharmacy, Second Military Medical University, Shanghai, 200433, China., Zhang DW; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China., Ma D; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China., Li ZT; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China. Electronic address: ztli@mail.sioc.ac.cn.

المصدر: Biomaterials [Biomaterials] 2022 May; Vol. 284, pp. 121467. Date of Electronic Publication: 2022 Mar 18.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE

مواضيع طبية MeSH: Neoplasms*/drug therapy , Photochemotherapy* , Porphyrins*/therapeutic use, Animals ; Humans ; Mice ; Photosensitizing Agents/pharmacology ; Photosensitizing Agents/therapeutic use ; Renal Dialysis

مستخلص: Despite that photodynamic therapy (PDT) has been applied for the treatment of cancer and skin diseases for more than two decades, all clinically used photodynamic agents (PDAs) suffer the drawback of skin phototoxicity of PDAs, which requires patients to avoid exposure to natural light for weeks after treatment, but has so far lacked effective suppression methods. Here, we report that three-dimensional diamondoid supramolecular organic frameworks (SOFs), that possess well-defined 2.1-nm porosity, can be used to suppress the skin phototoxicity of Photofrin, HiPorfin and Talaporfin, three porphyrin-based PDAs which clinically receive the most wide applications by injecting SOF after PDT, via an adsorption and retention mechanism. Fluorescence and dynamic light scattering experiments confirm that the SOFs have strong interaction with PDAs, and can adsorb PDAs at a micromolar concentration, whereas dialysis experiments support that the adsorption leads to an important retention effect. In vitro and in vivo experiments reveal that SOFs have high biocompatibility. Studies with healthy and tumor-bearing mouse models demonstrate that, when the PDAs are administrated at a dose comparable with the clinical one, SOF can remarkably suppress sunlight-induced skin phototoxicity, whereas the PDT efficacy of mice treated with SOF post-PDT is maintained. This work provides an efficient strategy for the improvement of the safety of clinically used PDAs.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)

المؤلفون: Xu ZY; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China. ztli@fudan.edu.cn., Mao W; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China. ztli@fudan.edu.cn., Zhao Z; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China. ztli@fudan.edu.cn., Wang ZK; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China. ztli@fudan.edu.cn., Liu YY; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China. ztli@fudan.edu.cn., Wu Y; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China. ztli@fudan.edu.cn., Wang H; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China. ztli@fudan.edu.cn., Zhang DW; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China. ztli@fudan.edu.cn., Li ZT; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China. ztli@fudan.edu.cn., Ma D; Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China. ztli@fudan.edu.cn.; School of Pharmaceutical and Materials Engineering & Institute for Advanced Studies, Taizhou University, 1139 Shifu Avenue, Jiaojiang 318000, Zhejiang, China.

المصدر: Journal of materials chemistry. B [J Mater Chem B] 2022 Feb 09; Vol. 10 (6), pp. 899-908. Date of Electronic Publication: 2022 Feb 09.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101598493 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-7518 (Electronic) Linking ISSN: 2050750X NLM ISO Abbreviation: J Mater Chem B Subsets: MEDLINE

مواضيع طبية MeSH: Nanoparticles* , Neoplasms*/drug therapy , Photochemotherapy*/methods, Animals ; Mesoporphyrins/therapeutic use ; Mice

مستخلص: Water-soluble three-dimensional supramolecular-organic frameworks (SOFs) and temoporfin ( m THPC) are discovered to form uniform self-assembled nanoparticles. These nanoparticles demonstrate an improved ¹ O 2 generation efficiency due to the reduced aggregation-caused quenching effect. SOFs and self-assembled nanoparticles are biocompatible. Self-assembled nanoparticles display an improved photo cytotoxicity toward four types of human cancer cells. The tumor model in mice shows that self-assembled nanoparticles could efficiently suppress tumor growth in vivo .

المؤلفون: Yang Y; MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, China.; College of Biophotonics, South China Normal University, Guangzhou, China., Zhang T; MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, China.; College of Biophotonics, South China Normal University, Guangzhou, China., Xing D; MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, China.; College of Biophotonics, South China Normal University, Guangzhou, China.

المصدر: Journal of biophotonics [J Biophotonics] 2021 Sep; Vol. 14 (9), pp. e202100134. Date of Electronic Publication: 2021 Jun 22.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101318567 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1864-0648 (Electronic) Linking ISSN: 1864063X NLM ISO Abbreviation: J Biophotonics Subsets: MEDLINE

مواضيع طبية MeSH: Nanocomposites* , Nanoparticles* , Neoplasms*/diagnostic imaging , Neoplasms*/drug therapy , Photochemotherapy*, Humans ; Photosensitizing Agents ; Phototherapy

مستخلص: Multifunctional phototheranostic nanocomposites are promising for early diagnosis and precision therapy of cancer. Aim to enhance their accuracy and efficiency, in this study, we develop a single-laser excited activatable phototheranostic nanocomposite (UCNPs-D-MQ): 808 nm-excited upconverting nanoparticles (UCNPs) as the matrix programmed assembly with amphipathic compound DSPE-PEG-COOH, a near-infrared absorbing polymer DPP and the pro-photosensitizer MBQB. Upon endocytosed by cancer cells and excited by the 808 nm laser, UCNPs-D-MQ could produce high-yield reactive oxygen species (ROS) as the results of singlet oxygen generation from transferring to methylene blue, GSH depletion and ROS generation from photoactivation. It was proven both in vitro and in vivo that the nanocomposites exhibits remarkable therapeutic efficacy as well as minimal photodamage to normal cells. These results reveal UCNPs-D-MQ as a robust theranostic agent for tumor phototherapy.
(© 2021 Wiley-VCH GmbH.)

المؤلفون: Zhang R; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China., Zeng Q; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China., Li X; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China., Xing D; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China. Electronic address: xingda@scnu.edu.cn., Zhang T; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China. Electronic address: zt@scnu.edu.cn.

المصدر: Biomaterials [Biomaterials] 2021 Aug; Vol. 275, pp. 120993. Date of Electronic Publication: 2021 Jun 28.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE

مواضيع طبية MeSH: Nanoparticles* , Photochemotherapy*, Cell Line, Tumor ; Diagnostic Imaging ; Gadolinium ; Oxygen ; Photosensitizing Agents/therapeutic use

مستخلص: Current light-mediated photodynamic therapy (PDT) is far underutilized in clinical cancer treatment due to its low pharmacological effect. We herein proposed a new gadolinium(III)-phthalocyanine (GdPc)-enabled phototherapeutics, photoacoustic/dynamic therapy (PADT), towards in vivo solid tumors via parallel-produced photocavitation and photodynamic oxidation with excitation by a single pulsed laser. We demonstrated that pulsed irradiation of GdPc could simultaneously produce an intense acoustic effect and a high-level ¹ O 2 quantum yield to afford mitochondrial damage and initiate programmed cell death. Under the guidance of magnetic resonance/photoacoustic dual-modal imaging, the mechanical oxygen-independent destruction of acoustic cavitation and the chemical damage of ¹ O 2 were validated to afford combinatorial inhibition of tumors under either normal or hypoxic conditions after the agent delivered into the cancer cells by a pH-sensitive nanomicelle. The single-laser initiated PADT using GdPc as a versatile photoagent maximizes the use of light energy to minimize the dose requirement of oxygen and agent towards high therapeutic efficacy, surpassing dramatically over conventional PDT.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)

المؤلفون: Yan S; Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China. lixionghn@csu.edu.cn liqinglonga@sina.com., Tang D, Hong Z, Wang J, Yao H, Lu L, Yi H, Fu S, Zheng C, He G, Zou H, Hou X, He Q, Xiong L, Li Q, Deng X

المصدر: Biomaterials science [Biomater Sci] 2021 Mar 21; Vol. 9 (6), pp. 2020-2031. Date of Electronic Publication: 2021 Jan 13.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101593571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2047-4849 (Electronic) Linking ISSN: 20474830 NLM ISO Abbreviation: Biomater Sci Subsets: MEDLINE

مواضيع طبية MeSH: Colorectal Neoplasms*/drug therapy , Photochemotherapy*, Animals ; Cell Line, Tumor ; Humans ; Mice ; Mice, Nude ; Neoplastic Stem Cells ; Peptides/therapeutic use ; Photosensitizing Agents/therapeutic use

مستخلص: Colorectal cancer (CRC) is the third most common cancer around the world. Recent findings suggest that cancer stem cells (CSCs) play a pivotal role in the resistance to current therapeutic modalities, including surgery and chemotherapy. Photodynamic therapy (PDT) is a promising non-invasive therapeutic strategy for advanced metastatic CRC. Traditional photosensitizers such as pyropheophorbide-a (Pyro) lack tumor selectivity, causing unwanted treatment-related toxicity to the surrounding normal tissue. In order to enhance the targeting properties of Pyro, we synthesize a novel photosensitizer, CD133-Pyro, via the conjugation of Pyro to a peptide domain targeting CD133, which is highly expressed on CRC CSCs and correlated with poor prognosis of CRC patients. We demonstrate that CD133-Pyro possesses the targeted delivery capacity both in CRC CSCs derived from HT29 and SW620 cell lines and in a xenograft mouse model of tumor growth. CD133-Pyro PDT can promote the production of reactive oxygen species (ROS), suppress the stemness properties, and induce autophagic cell death in CRC CSCs. Furthermore, CD133-Pyro PDT has a potent inhibitory effect on CRC CSC-derived xenograft tumors in nude mice. These findings may offer a useful and important strategy for the treatment of CRC through targeting CSCs.