المؤلفون: Emans PJ; Department of Orthopaedic Surgery, Maastricht University Medical Centre, The Netherlands. Pj.Emans@orthop.unimaas.nl, Jansen EJ, van Iersel D, Welting TJ, Woodfield TB, Bulstra SK, Riesle J, van Rhijn LW, Kuijer R

المصدر: Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2013 Sep; Vol. 7 (9), pp. 751-6. Date of Electronic Publication: 2012 Mar 21.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: John Wiley & Sons Country of Publication: England NLM ID: 101308490 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-7005 (Electronic) Linking ISSN: 19326254 NLM ISO Abbreviation: J Tissue Eng Regen Med Subsets: MEDLINE

مواضيع طبية MeSH: Regeneration*, Bone and Bones/*pathology , Cartilage, Articular/*pathology , Tissue Engineering/*methods , Tissue Scaffolds/*chemistry, Animals ; Chondrocytes/cytology ; Female ; Osteoarthritis/pathology ; Polyesters/chemistry ; Polyethylene Glycols/chemistry ; Polyethylene Terephthalates ; Polymers/chemistry ; Porosity ; Rabbits ; Wound Healing

مستخلص: Cartilage has a poor regenerative capacity. Tissue-engineering approaches using porous scaffolds seeded with chondrocytes may improve cartilage repair. The aim of this study was to examine the effect of pore size and pore interconnectivity on cartilage repair in osteochondral defects treated with different scaffolds seeded with allogenic chondrocytes. Scaffolds consisting of 55 wt% poly(ethylene oxide terephthalate) and 45 wt% poly(butylene terephthalate) (PEOT/PBT) with different pore sizes and interconnectivities were made, using a compression moulding (CM) and a three-dimensional fibre (3DF) deposition technique. In these scaffolds, allogenic chondrocytes were seeded, cultured for 3 weeks and implanted in osteochondral defects of skeletally mature rabbits. At 3 weeks no difference in cartilage repair between an empty osteochondral defect, CM or 3DF scaffolds was found. Three months post-implantation, cartilage repair was significantly improved after implantation of a 3DF scaffold compared to a CM scaffold. Although not significant, Mankin scores for osteoarthritis (OA) indicated less OA in the 3DF scaffold group compared to empty defects and CM-treated defects. It is concluded that scaffold pore size and pore interconnectivity influences osteochondral repair and a decreased pore interconnectivity seems to impair osteochondral repair.
(Copyright © 2012 John Wiley & Sons, Ltd.)

المؤلفون: Emans PJ; Department of Orthopedic Surgery, Maastricht University Medical Centre, Maastricht, 6200 MD, Netherlands. Pj.Emans@orthop.unimaas.nl, van Rhijn LW, Welting TJ, Cremers A, Wijnands N, Spaapen F, Voncken JW, Shastri VP

المصدر: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2010 Feb 23; Vol. 107 (8), pp. 3418-23. Date of Electronic Publication: 2010 Feb 04.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE

مواضيع طبية MeSH: Chondrogenesis*, Cartilage, Articular/*growth & development , Tissue Engineering/*methods, Anaerobiosis ; Animals ; Bioreactors ; Cartilage, Articular/cytology ; Cartilage, Articular/metabolism ; Cell Hypoxia ; Collagen Type II/biosynthesis ; Osteochondritis/surgery ; Rabbits ; Tissue Transplantation

مستخلص: Treatment of full-thickness damage to hyaline cartilage is hampered by the limited availability of autologous healthy cartilage and the lengthy, cost-prohibitive cell isolation and expansion steps associated with autologous cartilage implantation (ACI). Here we report a strategy for de novo engineering of ectopic autologous cartilage (EAC) within the subperiosteal space (in vivo bioreactor), through the mere introduction of a biocompatible gel that might promote hypoxia-mediated chondrogenesis, thereby effectively overcoming the aforementioned limitations. The EAC is obtained within 3 wk post injection of the gel, and can be press-fit into an osteochondral defect where it undergoes remodeling with good lateral and subchondral integration. The implanted EAC showed no calcification even after 9 mo and attained an average O'Driscoll score of 11 (versus 4 for controls). An "on demand" autologous source of autologous cartilage with remodeling capacity is expected to significantly impact the clinical options in repair of trauma to articular cartilage.

المؤلفون: Jansen EJ; Department of Orthopaedic Surgery, University Hospital Maastricht, Maastricht, The Netherlands. ejpjansen@hotmail.com, Emans PJ, Guldemond NA, van Rhijn LW, Welting TJ, Bulstra SK, Kuijer R

المصدر: Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2008 Aug; Vol. 2 (6), pp. 331-9.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: John Wiley & Sons Country of Publication: England NLM ID: 101308490 Publication Model: Print Cited Medium: Print ISSN: 1932-6254 (Print) Linking ISSN: 19326254 NLM ISO Abbreviation: J Tissue Eng Regen Med Subsets: MEDLINE

مواضيع طبية MeSH: Tissue Engineering*, Cartilage/*cytology , Cell Culture Techniques/*methods , Cell Separation/*methods , Periosteum/*cytology, Adult ; Aged ; Biomarkers ; Cartilage/metabolism ; Cell Proliferation ; Cells, Cultured ; Chondrogenesis ; Female ; Humans ; Middle Aged ; Periosteum/metabolism

مستخلص: The aim of this study was to establish the potential of human periosteum-derived cells from elderly patients as a cell source for cartilage tissue engineering by optimizing culture conditions for both proliferation and differentiation. Periosteum was obtained from the tibiae of nine patients. Biopsies were prepared for routine histological examination. Periosteum-derived cells were allowed to grow out from the remaining tissue, and were expanded in minimum essential medium containing D-valine (MEM-DV). Fetal bovine serum (FBS) or substitutes, fibroblast growth factor-2 (FGF-2), insulin-like growth factor-1 (IGF-1) and non-essential amino acids were added to study proliferation. For differentiation of cells, serum-free medium was used supplemented with one or more isoforms of transforming growth factor-beta (TGFbeta) and/or IGF-1. Samples were analysed for expression of collagens type I, II and X by competitive RT-PCR, immunohistochemically, and histologically using Alcian blue staining. In all samples the cambium layer could hardly be detected. Periosteum-derived cells proliferated in serum-containing MEM-DV. Optimal proliferation was found when this medium was supplemented with 100 ng/ml FGF-2 and non-essential amino acids. Chondrogenesis was detected in 59% of micromasses that were cultured with TGFbeta isomers, and in 83% of the samples cultured in media to which two TGFbeta isoforms were added. Periosteum from elderly humans (mean age 66, range 41-76 years) has chondrogenic potential and remains an attractive cell source for cartilage tissue engineering. By expanding cells in MEM-DV, the selection of progenitor cells might be favoured, which would result in a higher cartilage yield for tissue engineering applications.
((c) 2008 John Wiley & Sons, Ltd.)