المؤلفون: Tremblay J; Energy, Mining and Environment, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec, H4P2R2, Canada., Fortin N; Energy, Mining and Environment, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec, H4P2R2, Canada., Elias M; Energy, Mining and Environment, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec, H4P2R2, Canada., Wasserscheid J; Energy, Mining and Environment, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec, H4P2R2, Canada., King TL; Centre for Offshore Oil, Gas and Energy Research (COOGER), Fisheries and Oceans Canada, Dartmouth, Nova Scotia, B2Y4A2, Canada., Lee K; Fisheries and Oceans Canada, Bedford Institute of Oceanography, PO Box 1006, Dartmouth, Nova Scotia, B2Y4A2, Canada., Greer CW; Energy, Mining and Environment, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec, H4P2R2, Canada.

المصدر: Environmental microbiology [Environ Microbiol] 2019 Jul; Vol. 21 (7), pp. 2307-2319. Date of Electronic Publication: 2019 Apr 25.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Blackwell Science Country of Publication: England NLM ID: 100883692 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1462-2920 (Electronic) Linking ISSN: 14622912 NLM ISO Abbreviation: Environ Microbiol Subsets: MEDLINE

مواضيع طبية MeSH: Biodegradation, Environmental*, Oceanospirillaceae/*genetics , Oceanospirillaceae/*metabolism , Petroleum/*metabolism , Petroleum Pollution/*analysis, Alkanes/metabolism ; Canada ; Metagenome/genetics ; Seawater/microbiology ; Water Pollutants, Chemical/metabolism

مستخلص: Oil biodegradation has been extensively studied in the wake of the deepwater horizon spill, but the application of dispersant to oil spills in marine environments remains controversial. Here, we report metagenomic (MG) and metatranscriptomic (MT) data mining from microcosm experiments investigating the oil degrading potential of Canadian west and east coasts to estimate the gene abundance and activity of oil degrading bacteria in the presence of dispersant. We found that the addition of dispersant to crude oil mainly favours the abundance of Thalassolituus in the summer and Oleispira in the winter, two key natural oil degrading bacteria. We found a high abundance of genes related not only to n-alkane and aromatics degradation but also associated with transporters, two-component systems, bacterial motility, secretion systems and bacterial chemotaxis.
(© 2019 Her Majesty the Queen in Right of Canada Environmental Microbiology © 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.)

المؤلفون: Jensen S; Department of Aquaculture, Institute of Marine Research, Nordnes, Bergen, Norway. sigmund.jensen@imr.no, Ovreås L, Bergh O, Torsvik V

المصدر: Systematic and applied microbiology [Syst Appl Microbiol] 2004 Nov; Vol. 27 (6), pp. 728-36.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't

بيانات الدورية: Publisher: Elsevier GmbH Country of Publication: Germany NLM ID: 8306133 Publication Model: Print Cited Medium: Print ISSN: 0723-2020 (Print) Linking ISSN: 07232020 NLM ISO Abbreviation: Syst Appl Microbiol Subsets: MEDLINE

مواضيع طبية MeSH: Bacteria/*classification , Bacteria/*isolation & purification , Flounder/*microbiology , Larva/*microbiology, Animals ; Biofilms ; Canada ; DNA Fingerprinting ; DNA, Bacterial/analysis ; DNA, Bacterial/chemistry ; DNA, Bacterial/isolation & purification ; DNA, Ribosomal/chemistry ; DNA, Ribosomal/isolation & purification ; Genes, rRNA ; Iceland ; Molecular Sequence Data ; Norway ; Nucleic Acid Denaturation ; Oceanospirillaceae/classification ; Oceanospirillaceae/isolation & purification ; Oxalobacteraceae/classification ; Oxalobacteraceae/isolation & purification ; Phylogeny ; Pseudomonas/classification ; Pseudomonas/isolation & purification ; RNA, Bacterial/genetics ; RNA, Ribosomal, 16S/genetics ; Scotland ; Seawater/microbiology ; Sequence Analysis, DNA ; Vibrio/classification ; Vibrio/isolation & purification ; Water Microbiology

مستخلص: Halibut, the largest of all flatfishes is a valuable species with a great potential for aquaculture. Bacteria play an important role in regulating the health of the early life stages. The present article is the first broad-range molecular analysis of bacterial communities in larvae of the Atlantic halibut (Hippoglossus hippoglossus). DNA was extracted from larvae, water and silo biofilm from hatcheries in Norway, Scotland, Iceland and Canada. Eubacterial 16S rRNA gene fragments were amplified by polymerase chain reaction (PCR) with broad-range primers. Sequences spanning the hyper variable V3 region representing individual bacterial species were separated into community profiles by denaturing gradient gel electrophoresis (DGGE). The profiles revealed simple communities after hatching and bacterial succession following growth. Sequencing and phylogenetic analysis of excised DGGE bands suggested aerobic heterotrophs related to groups of Pseudomonas, Janthinobacterium and possibly Marinomonas to be the primary colonisers of the larvae. After onset of feeding, fermentative species (Vibrio) were detected as well. Comparative analysis of bacterial communities from different geographical regions indicated that larvae of the Atlantic halibut possess a distinct and specific normal flora.