المؤلفون: Coutinho, Antonio, Damasceno, Uellington da C., Mascarenhas, Esau da S., Santos, Allan C. de S., da Silva, Joao E. B. T., Greve, Fabiola

المصدر: ICC 2023 - IEEE International Conference on Communications Communications, ICC 2023 - IEEE International Conference on. :622-627 May, 2023

Relation: ICC 2023 - IEEE International Conference on Communications

المؤلفون: Lee, T. S.^{Aff1, Aff2, Aff3, IDs10434024151494_cor1}, Li, I.^{Aff1, Aff3}, Peric, B., Saw, R. P. M.^{Aff1, Aff3, Aff5, Aff6}, Duprat, J. P., Bertolli, E., Spillane, J. B., van Leeuwen, B. L., Moncrieff, M., Sommariva, A., Allan, C. P., de Wilt, J. H. W., Jones, R. Pritchard-, Geh, J. L. C., Howle, J. R.^{Aff3, Aff16}, Spillane, A. J.^{Aff1, Aff2, Aff3, Aff6}

المصدر: Annals of Surgical Oncology. 31(6):4061-4070

المؤلفون: Carlos Hernández-Pérez, Marc Combalia, Sebastian Podlipnik, Noel C. F. Codella, Veronica Rotemberg, Allan C. Halpern, Ofer Reiter, Cristina Carrera, Alicia Barreiro, Brian Helba, Susana Puig, Veronica Vilaplana, Josep Malvehy

المصدر: Scientific Data, Vol 11, Iss 1, Pp 1-9 (2024)

مصطلحات موضوعية: Science

الوصف: Abstract Advancements in dermatological artificial intelligence research require high-quality and comprehensive datasets that mirror real-world clinical scenarios. We introduce a collection of 18,946 dermoscopic images spanning from 2010 to 2016, collated at the Hospital Clínic in Barcelona, Spain. The BCN20000 dataset aims to address the problem of unconstrained classification of dermoscopic images of skin cancer, including lesions in hard-to-diagnose locations such as those found in nails and mucosa, large lesions which do not fit in the aperture of the dermoscopy device, and hypo-pigmented lesions. Our dataset covers eight key diagnostic categories in dermoscopy, providing a diverse range of lesions for artificial intelligence model training. Furthermore, a ninth out-of-distribution (OOD) class is also present on the test set, comprised of lesions which could not be distinctively classified as any of the others. By providing a comprehensive collection of varied images, BCN20000 helps bridge the gap between the training data for machine learning models and the day-to-day practice of medical practitioners. Additionally, we present a set of baseline classifiers based on state-of-the-art neural networks, which can be extended by other researchers for further experimentation.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2052-4463

URL الوصول: https://doaj.org/article/b665849eb0f243a2a69fd746dd408ed9

المؤلفون: Lee, T. S.^{Aff1, Aff2, Aff3, IDs10434024152624_cor1}, Li, I.^{Aff1, Aff3}, Peric, B., Saw, R. P. M.^{Aff1, Aff3, Aff5, Aff6}, Duprat, J. P., Bertolli, E., Spillane, J. B., van Leeuwen, B. L., Moncrieff, M., Sommariva, A., Allan, C., de Wilt, J. H. W., Pritchard-Jones, R., Geh, J. L. C., Howle, J. R.^{Aff3, Aff16}, Spillane, A. J.^{Aff1, Aff2, Aff3, Aff6}

المصدر: Annals of Surgical Oncology. 31(7):4609-4610

المؤلفون: Seaton, Kelly E, Huang, Yunda, Karuna, Shelly, Heptinstall, Jack R, Brackett, Caroline, Chiong, Kelvin, Zhang, Lily, Yates, Nicole L, Sampson, Mark, Rudnicki, Erika, Juraska, Michal, deCamp, Allan C, Edlefsen, Paul T, Mullins, James I, Williamson, Carolyn, Rossenkhan, Raabya, Giorgi, Elena E, Kenny, Avi, Angier, Heather, Randhawa, April, Weiner, Joshua A, Rojas, Michelle, Sarzotti-Kelsoe, Marcella, Zhang, Lu, Sawant, Sheetal, Ackerman, Margaret E, McDermott, Adrian B, Mascola, John R, Hural, John, McElrath, M Julianna, Andrew, Philip, Hidalgo, Jose A, Clark, Jesse, Laher, Fatima, Orrell, Catherine, Frank, Ian, Gonzales, Pedro, Edupuganti, Srilatha, Mgodi, Nyaradzo, Corey, Lawrence, Morris, Lynn, Montefiori, David, Cohen, Myron S, Gilbert, Peter B, Tomaras, Georgia D

مصطلحات موضوعية: Biomedical and Clinical Sciences, Immunology, Vaccine Related, Pediatric, Infectious Diseases, Vaccine Related (AIDS), HIV/AIDS, Prevention, Clinical Trials and Supportive Activities, Pediatric AIDS, Sexually Transmitted Infections, Clinical Research, Biotechnology, Immunization, Infection, Good Health and Well Being, Humans, Broadly Neutralizing Antibodies, Antibodies, Neutralizing, Acquired Immunodeficiency Syndrome, HIV Infections, HIV Seropositivity, HIV Antibodies, HIV-1, AIDS Vaccines, Body weight-based dosing, HIV, Broadly neutralising antibodies, body weight, prevention, broadly neutralizing antibodies, monoclonal antibody, pharmacokinetics, Clinical Sciences, Public Health and Health Services, Clinical sciences, Epidemiology

الوصف: BackgroundThe phase 2b proof-of-concept Antibody Mediated Prevention (AMP) trials showed that VRC01, an anti-HIV-1 broadly neutralising antibody (bnAb), prevented acquisition of HIV-1 sensitive to VRC01. To inform future study design and dosing regimen selection of candidate bnAbs, we investigated the association of VRC01 serum concentration with HIV-1 acquisition using AMP trial data.MethodsThe case-control sample included 107 VRC01 recipients who acquired HIV-1 and 82 VRC01 recipients who remained without HIV-1 during the study. We measured VRC01 serum concentrations with a qualified pharmacokinetic (PK) Binding Antibody Multiplex Assay. We employed nonlinear mixed effects PK modelling to estimate daily-grid VRC01 concentrations. Cox regression models were used to assess the association of VRC01 concentration at exposure and baseline body weight, with the hazard of HIV-1 acquisition and prevention efficacy as a function of VRC01 concentration. We also compared fixed dosing vs. body weight-based dosing via simulations.FindingsEstimated VRC01 concentrations in VRC01 recipients without HIV-1 were higher than those in VRC01 recipients who acquired HIV-1. Body weight was inversely associated with HIV-1 acquisition among both placebo and VRC01 recipients but did not modify the prevention efficacy of VRC01. VRC01 concentration was inversely correlated with HIV-1 acquisition, and positively correlated with prevention efficacy of VRC01. Simulation studies suggest that fixed dosing may be comparable to weight-based dosing in overall predicted prevention efficacy.InterpretationThese findings suggest that bnAb serum concentration may be a useful marker for dosing regimen selection, and operationally efficient fixed dosing regimens could be considered for future trials of HIV-1 bnAbs.FundingWas provided by the National Institutes of Health, National Institute of Allergy and Infectious Diseases (NIAID) (UM1 AI068614, to the HIV Vaccine Trials Network [HVTN]; UM1 AI068635, to the HVTN Statistical Data and Management Center [SDMC], Fred Hutchinson Cancer Center [FHCC]; 2R37 054165 to the FHCC; UM1 AI068618, to HVTN Laboratory Center, FHCC; UM1 AI068619, to the HPTN Leadership and Operations Center; UM1 AI068613, to the HIV Prevention Trials Network [HPTN] Laboratory Center; UM1 AI068617, to the HPTN SDMC; and P30 AI027757, to the Center for AIDS Research, Duke University (AI P30 AI064518) and University of Washington (P30 AI027757) Centers for AIDS Research; R37AI054165 from NIAID to the FHCC; and OPP1032144 CA-VIMC Bill & Melinda Gates Foundation.

وصف الملف: application/pdf

URL الوصول: https://escholarship.org/uc/item/4t81034b
https://escholarship.org/content/qt4t81034b/qt4t81034b.pdf

المؤلفون: Spradling, Allan C.^Aff5

المساهمون: Kubiak, Jacek Z., Series Editor^Aff1, Kloc, Malgorzata, Series Editor^{Aff2, Aff3}, Uosef, Ahmed, editor^Aff4

المصدر: Syncytia: Origin, Structure, and Functions. 71:3-21

المؤلفون: Ana Aragón-González, Allan C Shaw, Jannigje R Kok, Florence S Roussel, Cleide dos Santos Souza, Sarah M Granger, Tatyana Vetter, Yolanda de Diego, Kathrin C Meyer, Selina N Beal, Pamela J Shaw, Laura Ferraiuolo

المصدر: Fluids and Barriers of the CNS, Vol 21, Iss 1, Pp 1-14 (2024)

مصطلحات موضوعية: Stem cells, Blood brain barrier, Amyotrophic lateral sclerosis, C9ORF72, In vitro modelling, Neurodegeneration, Neurology. Diseases of the nervous system, RC346-429

الوصف: Abstract The blood-brain barrier (BBB) serves as a highly intricate and dynamic interface connecting the brain and the bloodstream, playing a vital role in maintaining brain homeostasis. BBB dysfunction has been associated with multiple neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS); however, the role of the BBB in neurodegeneration is understudied. We developed an ALS patient-derived model of the BBB by using cells derived from 5 patient donors carrying C9ORF72 mutations. Brain microvascular endothelial-like cells (BMEC-like cells) derived from C9ORF72-ALS patients showed altered gene expression, compromised barrier integrity, and increased P-glycoprotein transporter activity. In addition, mitochondrial metabolic tests demonstrated that C9ORF72-ALS BMECs display a significant decrease in basal glycolysis accompanied by increased basal and ATP-linked respiration. Moreover, our study reveals that C9-ALS derived astrocytes can further affect BMECs function and affect the expression of the glucose transporter Glut-1. Finally, C9ORF72 patient-derived BMECs form leaky barriers through a cell-autonomous mechanism and have neurotoxic properties towards motor neurons. Graphical Abstract

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2045-8118

URL الوصول: https://doaj.org/article/3b68b76102ee4ab192145d471b0955af

المؤلفون: Allan C. deCamp, Martin M. Corcoran, William J. Fulp, Jordan R. Willis, Christopher A. Cottrell, Daniel L. V. Bader, Oleksandr Kalyuzhniy, David J. Leggat, Kristen W. Cohen, Ollivier Hyrien, Sergey Menis, Greg Finak, Lamar Ballweber-Fleming, Abhinaya Srikanth, Jason R. Plyler, Farhad Rahaman, Angela Lombardo, Vincent Philiponis, Rachael E. Whaley, Aaron Seese, Joshua Brand, Alexis M. Ruppel, Wesley Hoyland, Celia R. Mahoney, Alberto Cagigi, Alison Taylor, David M. Brown, David R. Ambrozak, Troy Sincomb, Tina-Marie Mullen, Janine Maenza, Orpheus Kolokythas, Nadia Khati, Jeffrey Bethony, Mario Roederer, David Diemert, Richard A. Koup, Dagna S. Laufer, Juliana M. McElrath, Adrian B. McDermott, Gunilla B. Karlsson Hedestam, William R. Schief

المصدر: npj Vaccines, Vol 9, Iss 1, Pp 1-13 (2024)

مصطلحات موضوعية: Immunologic diseases. Allergy, RC581-607, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Vaccine priming immunogens that activate germline precursors for broadly neutralizing antibodies (bnAbs) have promise for development of precision vaccines against major human pathogens. In a clinical trial of the eOD-GT8 60mer germline-targeting immunogen, higher frequencies of vaccine-induced VRC01-class bnAb-precursor B cells were observed in the high dose compared to the low dose group. Through immunoglobulin heavy chain variable (IGHV) genotyping, statistical modeling, quantification of IGHV1-2 allele usage and B cell frequencies in the naive repertoire for each trial participant, and antibody affinity analyses, we found that the difference between dose groups in VRC01-class response frequency was best explained by IGHV1-2 genotype rather than dose and was most likely due to differences in IGHV1-2 B cell frequencies for different genotypes. The results demonstrate the need to define population-level immunoglobulin allelic variations when designing germline-targeting immunogens and evaluating them in clinical trials.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2059-0105

URL الوصول: https://doaj.org/article/2e580eabca0b4c0f8e97acb243086a89

المؤلفون: Craig A. Magaret, Li Li, Allan C. deCamp, Morgane Rolland, Michal Juraska, Brian D. Williamson, James Ludwig, Cindy Molitor, David Benkeser, Alex Luedtke, Brian Simpkins, Fei Heng, Yanqing Sun, Lindsay N. Carpp, Hongjun Bai, Bethany L. Dearlove, Elena E. Giorgi, Mandy Jongeneelen, Boerries Brandenburg, Matthew McCallum, John E. Bowen, David Veesler, Jerald Sadoff, Glenda E. Gray, Sanne Roels, An Vandebosch, Daniel J. Stieh, Mathieu Le Gars, Johan Vingerhoets, Beatriz Grinsztejn, Paul A. Goepfert, Leonardo Paiva de Sousa, Mayara Secco Torres Silva, Martin Casapia, Marcelo H. Losso, Susan J. Little, Aditya Gaur, Linda-Gail Bekker, Nigel Garrett, Carla Truyers, Ilse Van Dromme, Edith Swann, Mary A. Marovich, Dean Follmann, Kathleen M. Neuzil, Lawrence Corey, Alexander L. Greninger, Pavitra Roychoudhury, Ollivier Hyrien, Peter B. Gilbert

المصدر: Nature Communications, Vol 15, Iss 1, Pp 1-22 (2024)

مصطلحات موضوعية: Science

الوصف: Abstract In the ENSEMBLE randomized, placebo-controlled phase 3 trial (NCT04505722), estimated single-dose Ad26.COV2.S vaccine efficacy (VE) was 56% against moderate to severe–critical COVID-19. SARS-CoV-2 Spike sequences were determined from 484 vaccine and 1,067 placebo recipients who acquired COVID-19. In this set of prespecified analyses, we show that in Latin America, VE was significantly lower against Lambda vs. Reference and against Lambda vs. non-Lambda [family-wise error rate (FWER) p

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2041-1723

URL الوصول: https://doaj.org/article/ae82624b40e944a5925f8f3c70ded9b7

المؤلفون: Dodge, Ren, Jones, Eric W, Zhu, Haolong, Obadia, Benjamin, Martinez, Daniel J, Wang, Chenhui, Aranda-Díaz, Andrés, Aumiller, Kevin, Liu, Zhexian, Voltolini, Marco, Brodie, Eoin L, Huang, Kerwyn Casey, Carlson, Jean M, Sivak, David A, Spradling, Allan C, Ludington, William B

المصدر: Nature Communications. 14(1)

مصطلحات موضوعية: Microbiology, Climate Change Impacts and Adaptation, Biological Sciences, Environmental Sciences, Nutrition, Prevention, Infectious Diseases, Digestive Diseases, 1.1 Normal biological development and functioning, Underpinning research, Animals, Drosophila melanogaster, Gastrointestinal Microbiome, Gastrointestinal Tract, Microbiota, Bacteria, Drosophila, Mammals

الوصف: The gut is continuously invaded by diverse bacteria from the diet and the environment, yet microbiome composition is relatively stable over time for host species ranging from mammals to insects, suggesting host-specific factors may selectively maintain key species of bacteria. To investigate host specificity, we used gnotobiotic Drosophila, microbial pulse-chase protocols, and microscopy to investigate the stability of different strains of bacteria in the fly gut. We show that a host-constructed physical niche in the foregut selectively binds bacteria with strain-level specificity, stabilizing their colonization. Primary colonizers saturate the niche and exclude secondary colonizers of the same strain, but initial colonization by Lactobacillus species physically remodels the niche through production of a glycan-rich secretion to favor secondary colonization by unrelated commensals in the Acetobacter genus. Our results provide a mechanistic framework for understanding the establishment and stability of a multi-species intestinal microbiome.

وصف الملف: application/pdf

URL الوصول: https://escholarship.org/uc/item/02h735d3