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1دورية أكاديمية
المؤلفون: Seiji Saito, Utsugi Kanazawa, Ayana Tatsumi, Atsuo Iida, Tatsuya Takemoto, Takayuki Suzuki
المصدر: Frontiers in Cell and Developmental Biology, Vol 12 (2024)
مصطلحات موضوعية: Gdf11, enhancer, hindlimb, sacral vertebrae, morphogenesis, Biology (General), QH301-705.5
الوصف: During the early development of tetrapods, including humans, the embryonic body elongates caudally once the anterior-posterior axis is established. During this process, region-specific vertebral morphogenesis occurs, with the determination of limb positioning along the anterior-posterior axis. We previously reported that Gdf11 functions as an anatomical integration system that determines the positioning of hindlimbs and sacral vertebrae where Gdf11 is expressed. However, the molecular mechanisms underlying induction of Gdf11 expression remain unclear. In this study, we searched for non-coding regions near the Gdf11 locus that were conserved across species to elucidate the regulatory mechanisms of Gdf11 expression. We identified an enhancer of the Gdf11 gene in intron 1 and named it highly conserved region (HCR). In HCR knockout mice, the expression level of endogenous Gdf11 was decreased, and the position of the sacral-hindlimb unit was shifted posteriorly. We also searched for factors upstream of Gdf11 based on the predicted transcription factor binding sites within the HCR. We found that inhibition of FGF signaling increased endogenous Gdf11 expression, suggesting that FGF signaling negatively regulates Gdf11 expression. However, FGF signaling does not regulate HCR activity. Our results suggest that there are species-specific Gdf11 enhancers other than HCR and that FGF signaling regulates Gdf11 expression independent of HCR.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
المصدر: Virus Research, Vol 339, Iss , Pp 199248- (2024)
مصطلحات موضوعية: Pteropine orthoreovirus, Antiviral, Fda-approved drug, Micafungin, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216
الوصف: Bat-borne emerging zoonotic viruses cause major outbreaks, such as the Ebola virus, Nipah virus, and/or beta coronavirus. Pteropine orthoreovirus (PRV), whose spillover event occurred from fruits bats to humans, causes respiratory syndrome in humans widely in South East Asia. Repurposing approved drugs against PRV is an effective tool to confront future PRV pandemics. We screened 2,943 compounds in an FDA-approved drug library and identified eight hit compounds that reduce viral cytopathic effects on cultured Vero cells. Real-time quantitative PCR analysis revealed that six of eight hit compounds significantly inhibited PRV replication. Among them, micafungin used clinically as an antifungal drug, displayed a prominent antiviral effect on PRV. Secondly, the antiviral effects of micafungin on PRV infected human cell lines (HEK293T and A549), and their transcriptome changes by PRV infection were investigated, compared to four different bat-derived cell lines (FBKT1 (Ryukyu flying fox), DEMKT1 (Leschenault's rousette), BKT1 (Greater horseshoe bat), YUBFKT1 (Eastern bent-wing bats)). In two human cell lines, unlike bat cells that induce an IFN-γ response pathway, an endoplasmic reticulum stress response pathway was commonly activated. Additionally, micafungin inhibits viral release rather than suppressing PRV genome replication in human cells, although it was disturbed in Vero cells. The target of micafungin's action may vary depending on the animal species, but it must be useful for human purposes as a first choice of medical care.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Shintaro Sakaki, Reo Ito, Hideki Abe, Masato Kinoshita, Eiichi Hondo, Atsuo Iida
المصدر: PeerJ, Vol 11, p e16596 (2023)
مصطلحات موضوعية: Electric eel, Electroporation, Fluorescent protein, Gene transfer, Zebrafish, Electric organ discharge, Medicine, Biology (General), QH301-705.5
الوصف: Background Electric eels (Electrophorus sp.) are known for their ability to produce electric organ discharge (EOD) reaching voltages of up to 860 V. Given that gene transfer via intense electrical pulses is a well-established technique in genetic engineering, we hypothesized that electric eels could potentially function as a gene transfer mechanism in their aquatic environment. Methods To investigate this hypothesis, we immersed zebrafish larvae in water containing DNA encoding the green fluorescent protein (GFP) and exposed them to electric eel’s EOD. Results and Discussion Some embryos exhibited a mosaic expression of green fluorescence, in contrast to the control group without electrical stimulation, which showed little distinct fluorescence. This suggests that electric eel EOD has the potential to function as an electroporator for the transfer of DNA into eukaryotic cells. While electric eel EOD is primarily associated with behaviors related to sensing, predation, and defense, it may incidentally serve as a possible mechanism for gene transfer in natural environment. This investigation represents the initial exploration of the uncharted impact of electric eel EOD, but it does not directly establish its significance within the natural environment. Further research is required to understand the ecological implications of this phenomenon.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Thachawech Kimprasit, Mitsuo Nunome, Keisuke Iida, Yoshitaka Murakami, Min-Liang Wong, Chung-Hsin Wu, Ryosuke Kobayashi, Yupadee Hengjan, Hitoshi Takemae, Kenzo Yonemitsu, Ryusei Kuwata, Hiroshi Shimoda, Lifan Si, Joon-Hyuk Sohn, Susumu Asakawa, Kenji Ichiyanagi, Ken Maeda, Hong-Shik Oh, Tetsuya Mizutani, Junpei Kimura, Atsuo Iida, Eiichi Hondo
المصدر: PLoS ONE, Vol 16, Iss 1, p e0244006 (2021)
الوصف: In this study, we examined the role of the eastern bent-winged bat (Miniopterus fuliginosus) in the dispersion of bat adenovirus and bat alphacoronavirus in east Asia, considering their gene flows and divergence times (based on deep-sequencing data), using bat fecal guano samples. Bats in China moved to Jeju Island and/or Taiwan in the last 20,000 years via the Korean Peninsula and/or Japan. The phylogenies of host mitochondrial D-loop DNA was not significantly congruent with those of bat adenovirus (m2XY = 0.07, p = 0.08), and bat alphacoronavirus (m2XY = 0.48, p = 0.20). We estimate that the first divergence time of bats carrying bat adenovirus in five caves studied (designated as K1, K2, JJ, N2, and F3) occurred approximately 3.17 million years ago. In contrast, the first divergence time of bat adenovirus among bats in the 5 caves was estimated to be approximately 224.32 years ago. The first divergence time of bats in caves CH, JJ, WY, N2, F1, F2, and F3 harboring bat alphacoronavirus was estimated to be 1.59 million years ago. The first divergence time of bat alphacoronavirus among the 7 caves was estimated to be approximately 2,596.92 years ago. The origin of bat adenovirus remains unclear, whereas our findings suggest that bat alphacoronavirus originated in Japan. Surprisingly, bat adenovirus and bat alphacoronavirus appeared to diverge substantially over the last 100 years, even though our gene-flow data indicate that the eastern bent-winged bat serves as an important natural reservoir of both viruses.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/1932-6203
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5دورية أكاديمية
المؤلفون: Taihei Mukaide, Atsuo Iida, Masatoshi Watanabe, Kazuhiro Takada, Takashi Noma
المصدر: PLoS ONE, Vol 10, Iss 6, p e0131401 (2015)
الوصف: We demonstrate a new analytical X-ray computed tomography technique for visualizing and quantifying the mass density of materials comprised of low atomic number elements with unknown atomic ratios. The mass density was obtained from the experimentally observed ratio of the imaginary and real parts of the complex X-ray refractive index. An empirical linear relationship between the X-ray mass attenuation coefficient of the materials and X-ray energy was found for X-ray energies between 8 keV and 30 keV. The mass density image of two polymer fibers was quantified using the proposed technique using a scanning-type X-ray microbeam computed tomography system equipped with a wedge absorber. The reconstructed mass density agrees well with the calculated one.
وصف الملف: electronic resource
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6دورية أكاديمية
المؤلفون: Ratanayotha, Adisorn, Atsuo Iida, Jumpei Nomura, Eiichi Hondo, Yasushi Okamura, Takafumi Kawai
المصدر: American Journal of Physiology: Regulatory, Integrative & Comparative Physiology; Jun2024, Vol. 326 Issue 6, p461-671, 11p
مستخلص: Nutrient absorption is essential for animal survival and development. Our previous study on zebrafish reported that nutrient absorption in lysosome-rich enterocytes (LREs) is promoted by the voltage-sensing phosphatase (VSP), which regulates phosphoinositide (PIP) homeostasis via electrical signaling in biological membranes. However, it remains unknown whether this VSP function is shared by different absorptive tissues in other species. Here, we focused on the function of VSP in a viviparous teleost Xenotoca eiseni, whose intraovarian embryos absorb nutrients from the maternal ovarian fluid through a specialized hindgutderived pseudoplacental structure called trophotaenia. Xenotoca eiseni VSP (Xe-VSP) is expressed in trophotaenia epithelium, an absorptive tissue functionally similar to zebrafish LREs. Notably, the apical distribution of Xe-VSP in trophotaenia epithelial cells closely resembles zebrafish VSP (Dr-VSP) distribution in zebrafish LREs, suggesting a shared role for VSP in absorptive tissues between the two species. Electrophysiological analysis using a heterologous expression system revealed that Xe-VSP preserves functional voltage sensors and phosphatase activity with the leftward shifted voltage sensitivity compared with zebrafish VSP (Dr-VSP). We also identified a single amino acid variation in the S4 helix of Xe-VSP as one of the factors contributing to the leftward shifted voltage sensitivity. This study highlights the biological variation and significance of VSP in various animal species, as well as hinting at the potential role of VSP in nutrient absorption in X. eiseni trophotaenia. NEW & NOTEWORTHY We investigate the voltage-sensing phosphatase (VSP) in Xenotoca eiseni, a viviparous fish whose intraovarian embryos utilize trophotaenia for nutrient absorption. Although X. eiseni VSP (Xe-VSP) shares key features with known VSPs, its distinct voltage sensitivity arises from species-specific amino acid variation. Xe-VSP in trophotaenia epithelium suggests its involvement in nutrient absorption, similar to VSP in zebrafish enterocytes and potentially in species with similar absorptive cells. Our findings highlight the potential role of VSP across species. [ABSTRACT FROM AUTHOR]
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المؤلفون: Jumpei Nomura, Hayato Yokoi, Eiichi Hondo, Atsuo Iida
المصدر: Biochemical and Biophysical Research Communications. 644:79-84
مصطلحات موضوعية: Biophysics, Cell Biology, Molecular Biology, Biochemistry
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8
المؤلفون: Sakura Hayashi, Takuji Tsukiyama, Akihiko Koga, Atsuo Iida, Masato Kinoshita
المصدر: Genome. 65:183-187
مصطلحات موضوعية: Genetics, Transposable element, General Medicine, Biology, Transposition (music), chemistry.chemical_compound, chemistry, Horizontal gene transfer, Fish
, Identification (biology), Molecular Biology, Gene, DNA, Transposase, Biotechnology الوصف: The majority of DNA-based transposable elements comprise autonomous and nonautonomous copies, or only nonautonomous copies, where the autonomous copy contains an intact gene for a transposase protein and the nonautonomous copy does not. Even if autonomous copies coexist, they are generally less frequent. The Tol2 element of medaka fish is one of the few elements for which a nonautonomous copy has not yet been found. Here, we report the presence of a nonautonomous Tol2 copy that was identified by surveying the medaka genome sequence database. This copy contained three local sequence alterations that affected the deduced amino acid sequence of the transposase: a deletion of 15 nucleotides resulting in a deletion of 5 amino acids, a base substitution causing a single amino acid change, and another base substitution giving rise to a stop codon. Transposition assays using cultured human cells revealed that transposase activity was reduced by the 15-nucleotide deletion and abolished by the nonsense mutation. This is the first example of a nonautonomous Tol2 copy. Thus, Tol2 is in an early stage of decay in the medaka genome, and is therefore a unique element to observe an almost complete decay process that progresses in natural populations.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::81c7e1fcfce08b0e2cd7ff0f0c31b4fb
https://doi.org/10.1139/gen-2021-0010 -
9
المؤلفون: Eiichi Hondo, Ronald Tarigan, Tetsufumi Katta, Atsuo Iida, Ken Maeda, Hitoshi Takemae, Hiroshi Shimoda
المصدر: Virus Genes
مصطلحات موضوعية: Swine, animal diseases, viruses, Pattern recognition receptor, Porcine Reproductive and Respiratory Syndrome, Antiviral Agents, Cell Line, Interferon, Chiroptera, Virology, Genetics, medicine, Animals, Molecular Biology, Original Paper, Innate immune system, biology, Rhinolophus ferrumequinum, Bat, virus diseases, MDA5, General Medicine, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Orthoreovirus, Viral replication, Cell culture, Receptors, Pattern Recognition, TLR3, Interferons, Pteropine orthoreovirus, medicine.drug
الوصف: Bats serve as natural hosts of Pteropine orthoreovirus (PRV), an emerging group of bat-borne, zoonotic viruses. Bats appear to possess unique innate immune system responses that can inhibit viral replication, thus reducing clinical symptoms. We examined the innate immune response against PRV and assessed viral replication in cell lines derived from four bat species (Miniopterus fuliginosus, Pteropus dasymallus, Rhinolophus ferrumequinum, and Rousettus leschenaultii), one rodent (Mesocricetous auratus), and human (Homo sapiens). The expression levels of pattern recognition receptors (PRRs) (TLR3, RIG-I, and MDA5) and interferons (IFNB1 and IFNL1) were higher and PRV replication was lower in cell lines derived from M. fuliginosus, R. ferrumequinum, and R. leschenaultii. Reduction of IFNB1 expression by the knockdown of PRRs in the cell line derived from R. ferrumequinum was associated with increased PRV replication. The knockdown of RIG-I led to the most significant reduction in viral replication for all cell lines. These results suggest that RIG-I production is important for antiviral response against PRV in R. ferrumequinum. Supplementary Information The online version contains supplementary material available at 10.1007/s11262-021-01865-6.
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10
المؤلفون: Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Atsuo Iida, Eiichi Hondo
الوصف: Bat-borne emerging zoonotic viruses cause major outbreaks, such as the Ebola virus, Nipah virus, severe acute respiratory syndrome (SARS) coronavirus, and SARS-CoV-2. Pteropine orthoreovirus (PRV), which spillover event occurred from fruit bats to humans, causes respiratory syndrome in humans widely in South East Asia. Repurposing approved drugs against PRV is a critical tool to confront future PRV pandemics. We screened 2,943 compounds in an FDA-approved drug library and identified eight hit compounds that reduce viral cytopathic effects on cultured Vero cells. Real-time quantitative PCR analysis revealed that six of eight hit compounds significantly inhibited PRV replication. Among them, micafungin used clinically as an antifungal drug, displayed a prominent antiviral effect on PRV.HighlightsA library of 2,943 FDA-approved drugs was screened to find potential antiviral drugs of Pteropine orthoreovirus.Six hit compounds dramatically inhibited viral replication in vitro.Micafungin possessed antiviral activity to multiple strains of PRV.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::cf932c4b351d94ae275d381641f20641
https://doi.org/10.1101/2022.09.21.508823
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