المؤلفون: Netongo PM; Molecular Diagnostics Research Group, Biotechnology Centre-University of Yaounde I, Cameroon.; Department of Biochemistry, Faculty of Science, University of Yaounde I, Cameroon.; Laboratory for Public Health Research Biotechnologies, Biotechnology Centre, University of Yaounde I, Cameroon.; School of Science, Navajo Technical University, Crownpoint, New Mexico, USA., Kamdem SD; Molecular Diagnostics Research Group, Biotechnology Centre-University of Yaounde I, Cameroon.; School of Health Sciences, Catholic University of Central Africa, Yaoundé, Cameroon., Ouayoue AN; Molecular Diagnostics Research Group, Biotechnology Centre-University of Yaounde I, Cameroon.; Laboratory for Public Health Research Biotechnologies, Biotechnology Centre, University of Yaounde I, Cameroon., Djiokeng TP; School of Health Sciences, Catholic University of Central Africa, Yaoundé, Cameroon., Toju SW; School of Health Sciences, Catholic University of Central Africa, Yaoundé, Cameroon., Tchoupe EB; Molecular Diagnostics Research Group, Biotechnology Centre-University of Yaounde I, Cameroon.; Laboratory for Public Health Research Biotechnologies, Biotechnology Centre, University of Yaounde I, Cameroon., Djivida P; Molecular Diagnostics Research Group, Biotechnology Centre-University of Yaounde I, Cameroon.; Laboratory for Public Health Research Biotechnologies, Biotechnology Centre, University of Yaounde I, Cameroon., Chedjou JP; Department of Biochemistry, Faculty of Science, University of Yaounde I, Cameroon.; Laboratory for Public Health Research Biotechnologies, Biotechnology Centre, University of Yaounde I, Cameroon., Domkam I; Centre de Reference International Chantal Biya, Yaoundé, Cameroon., Mbacham W; Department of Biochemistry, Faculty of Science, University of Yaounde I, Cameroon.; Laboratory for Public Health Research Biotechnologies, Biotechnology Centre, University of Yaounde I, Cameroon.

المصدر: BioMed research international [Biomed Res Int] 2022 Nov 09; Vol. 2022, pp. 3600354. Date of Electronic Publication: 2022 Nov 09 (Print Publication: 2022).

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Hindawi Pub. Co Country of Publication: United States NLM ID: 101600173 Publication Model: eCollection Cited Medium: Internet ISSN: 2314-6141 (Electronic) NLM ISO Abbreviation: Biomed Res Int Subsets: MEDLINE

مواضيع طبية MeSH: Plasmodium falciparum*/genetics , Malaria*/diagnosis , Malaria*/epidemiology, Humans ; Infant ; Child, Preschool ; Child ; Adolescent ; Young Adult ; Adult ; Middle Aged ; Aged ; Cross-Sectional Studies ; Cameroon/epidemiology ; Sensitivity and Specificity ; Cost-Benefit Analysis

مستخلص: Background: Accurate, cost-effective, and noninvasive alternative molecular methods are needed for detecting low malaria parasitemia. The currently-used nested polymerase chain reaction (nPCR) requires blood as well as skilled personnel in order to minimise the risk of bloodborne disease transmission. Therefore, this study is aimed at assessing the accuracy of a noninvasive and more affordable malaria diagnosis with saliva using the loop-mediated isothermal amplification (LAMP) technique.
Methods: A cross-sectional study was conducted in the Centre and Southwest regions of Cameroon. Matched blood and saliva samples collected from symptomatic and asymptomatic participants were tested for malaria using rapid diagnostic tests, microscopy, PCR, and LAMP. Statistics were performed using R studio software at 95% confidence interval.
Results: A total of 100 participants (65% symptomatic and 35% asymptomatic) aged between 1 and 74 years with a balanced gender distribution ratio of 1.08 were included in our study. The prevalence of malaria was 61%, 57%, 59%, 42%, 35%, 17%, and 16% for blood-RDT, blood-PCR, blood-LAMP, blood-RT-LAMP, saliva-PCR, saliva-RT-LAMP, and saliva-LAMP, respectively. Both saliva and blood showed a sensitivity of 43.90% and respective specificities of 68.75% and 57.62%. When using RT-LAMP, sensitivities of 49.38% and 48.21% and specificities of 94.11% and 66.67% were recorded for saliva and blood, respectively. Sensitivities of 70.23% and 73.49% and specificities of 62.5% and 76.47% were recorded, respectively, for saliva-LAMP and saliva-RT-LAMP when compared to saliva-PCR as the gold standard. Saliva-LAMP and saliva-RT-LAMP had a fair agreement ( к = 0.221 and 0.352, respectively) with saliva-PCR. Homemade LAMP and RT-LAMP technologies match the WHO recommendations and after proper validation in a larger sample size, could serve for malaria diagnosis in developing countries.
Competing Interests: The authors declare that they have no competing interests.
(Copyright © 2022 Palmer Masumbe Netongo et al.)