المؤلفون: Guoliang Wang, Jinxiang Zhang, Hao Liu, Qichang Zheng, Ping Sun

المصدر: BMC Gastroenterology, Vol 24, Iss 1, Pp 1-9 (2024)

مصطلحات موضوعية: Hepatocellular carcinoma, Transarterial chemoembolization, Transarterial embolization, Meta-analysis, Diseases of the digestive system. Gastroenterology, RC799-869

الوصف: Abstract Background Despite transarterial chemoembolization (TACE) was recommended as first line therapy for intermediate hepatocellular carcinoma (HCC), the efficacy of transarterial embolization (TAE) has not been widely recognized. This work was to determine whether TAE was as effective and safe as TACE for unresectable HCC. Methods We performed a systematic search of electronic databases and other sources for randomized controlled studies (RCTs) comparing TAE with TACE for unresectable HCC. Results were expressed as Hazard Ratio (HR) for survival and Odds Ratio (OR) for dichotomous outcomes using RevMan 5.4.1. Results We included 6 trials with 683 patients. The risk of bias of included RCTs was from unclear to high risk. There were no significant differences between TACE and TAE for progression-free survival (HR 0.83, 95% CI 0.45–1.55; p = 0.57), overall survival (HR 1.10, 95% CI 0.90–1.35; p = 0.36), and objective response rate (OR 1.17, 95% CI 0.80–1.71; p = 0.42) without obvious publication bias. Sensitivity analyses confirmed the robustness of the results. TAE group reported similar or less adverse effects than TACE group in all the studies. Conclusions Our study demonstrated that TAE was as effective as TACE. Since TAE was simpler, cheaper and had less adverse effects than TACE, TAE should be a better choice in most cases where TACE was indicated for unresectable HCC.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/1471-230X

URL الوصول: https://doaj.org/article/be34bdd194114e7e979ea9c1f06f33e5

المؤلفون: Jiyuan Chen, Zhuo Liu, Ran Peng, Yunchong Liu, Hongxian Zhang, Guoliang Wang, Xiaojun Tian, Xinlong Pei, Junjie Wang, Shudong Zhang, Hao Wang, Lulin Ma

المصدر: BMC Urology, Vol 24, Iss 1, Pp 1-7 (2024)

مصطلحات موضوعية: Renal cell carcinoma, Radiotherapy, Neoadjuvant therapy, Venous tumor thrombus, Diseases of the genitourinary system. Urology, RC870-923

الوصف: Abstract Background Surgical treatment for renal cell carcinoma (RCC) and inferior vena cava (IVC) tumor thrombus (TT) is difficult, and the postoperative complication rate is high. This study aimed to explore the safety and oncologic outcomes of neoadjuvant stereotactic ablative body radiotherapy (SABR) combined with surgical treatment for RCC and IVC-TT. Methods Patients with RCC and IVC-TTs were enrolled in this study. All patients received neoadjuvant SABR focused on the IVC at a dose of 30 Gy in 5 fractions, followed by 2 ~ 4 weeks of rest. Then, radical nephrectomy and IVC tumor thrombectomy were performed for each patient. Adverse effects, perioperative outcomes, and long-term prognoses were recorded. Results From June 2018 to January 2019, 8 patients were enrolled—4 with Mayo grade II TT and 4 with Mayo grade III TT. Four (50%) patients had complicated IVC wall invasion according to CT/MRI. All patients received neoadjuvant SABR as planned. Short-term local control was observed in all 8 patients. Only Grade 1–2 adverse events were reported. In total, 3 (37.5%) laparoscopic surgeries and 5 (62.5%) open surgeries were performed. The median operation time was 359 (IQR: 279–446) min, with a median intraoperative bleeding volume of 750 (IQR: 275–2175) ml. The median postoperative hospital stay was 7 (5–10) days. With a 26-month (range: 5–41) follow-up period, the estimated mean overall survival was 30.67 ± 5.38 months. Conclusions This is the first preoperative radiotherapy study in Asia that focused on patients with TT. This study revealed the considerable safety of neoadjuvant SABR for RCC with IVC-TT. Trial registration This study was registered in the Chinese Clinical Trials Registry on 2018-03-08 (ChiCTR1800015118). For more information, please see the direct link ( https://www.chictr.org.cn/showproj.html?proj=25747 ).

وصف الملف: electronic resource

Relation: https://doaj.org/toc/1471-2490

URL الوصول: https://doaj.org/article/b7cf75cb68c24360ad8813783b569f3f

المؤلفون: Guoliang Wang, Tianping Ren

المصدر: Heliyon, Vol 10, Iss 10, Pp e30055- (2024)

مصطلحات موضوعية: Machine learning, U-Net convolutional neural network, Achievement prediction, Dense connection, Attention module, Residual learning, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Sports plays a pivotal role in national development. To accurately predict college students' sports performance and motivate them to improve their physical fitness, this study constructs a sports achievement prediction system by using a U-Net Convolutional Neural Network (CNN) in machine learning. Firstly, the current state of physical education teachers’ instructional proficiency is investigated and analyzed to identify existing problems. Secondly, an improved U-Net-based sports achievement prediction system is proposed. This method enhances the utilization and propagation of network features by incorporating dense connections, thus addressing gradient disappearance issues. Simultaneously, an improved mixed loss function is introduced to alleviate class imbalance. Finally, the effectiveness of the proposed system is validated through testing, demonstrating that the improved U-Net CNN algorithm yields superior results. Specifically, the prediction accuracy of the improved network for sports performance surpasses that of the original U-Net by 4.22 % and exceeds that of DUNet by 5.22 %. Compared with other existing prediction networks, the improved U-Net CNN model exhibits a superior achievement prediction ability. Consequently, the proposed system enhances teaching and learning efficiency and offers insights into applying artificial intelligence technology to smart classroom development.

وصف الملف: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2405844024060869; https://doaj.org/toc/2405-8440

URL الوصول: https://doaj.org/article/0922713e35614cdcab96e54c5bcf105d

المؤلفون: Hang Jing, Huiling Wang, Guoliang Wang, Guobin Liu, Yi Cheng

المصدر: Carbon Research, Vol 2, Iss 1, Pp 1-17 (2023)

مصطلحات موضوعية: Root exudate, Metabolomics, High-throughput sequencing, Bacteria, Fungi, Production of electric energy or power. Powerplants. Central stations, TK1001-1841, Environmental sciences, GE1-350

الوصف: Abstract Structures and functions were varied among roots with different diameters, and responded differently to nitrogen (N) addition, a phenomenon called hierarchical trait. However, the presence of this trait in carbon (C) metabolite of root exudate and rhizosphere microbial community remains unclear. We studied the patterns of C metabolites and microbial communities of rhizosphere soil among different diameter roots of Pinus tabulaeformis and their responses to N addition using metabolomics and high-throughput sequencing methods. Root C metabolites and rhizosphere soil microbial communities had hierarchical traits, in which exudates of R1 roots (0–0.5 mm) were rich in fatty acids and organic acids, while exudates of R3 roots (1–2 mm) were rich in carbohydrates and alcohols. With the increasing root diameters, numbers of bacteria and fungi of rhizosphere soil decreased and increased, respectively, while diversities of bacteria and fungi increased. N addition had a promotion effect on abundance of root C metabolites. Numbers of bacteria and fungi of rhizosphere soil were enhanced and inhibited, respectively, while diversities of bacteria and fungi were facilitated by N addition. N addition promoted microbial populations of rhizosphere soil by reducing exudates of R1 roots, and increasing exudates of R2 (0.5–1 mm) and R3 roots. Hippuric acid and cytidine-5´-monophosphate of R1 roots, lactic acid of R2 roots, glucose 1 and spermidine 2 of R3 roots were the functional C metabolites that affected microbial populations of rhizosphere soil, such as Actinobacteria, Acidobacteria, Proteobacteria, Basidiomycota, and Ascomycota. Hierarchical traits were extended from root to rhizosphere soil in this study. Graphical Abstract

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2731-6696

URL الوصول: https://doaj.org/article/301d041a295747d580f0e3ca6ab5a600

المؤلفون: Zhuang Xiong, Lizhi Li, Guoliang Wang, Lei Guo, Shangyi Luo, Xiangwen Liao, Jingfeng Liu, Wenhao Teng

المصدر: Genes, Vol 15, Iss 6, p 755 (2024)

مصطلحات موضوعية: liver cancer, metabolic reprogramming, single-cell RNA-seq, prognostic risk model, Genetics, QH426-470

الوصف: Liver cancer manifests as a profoundly heterogeneous malignancy, posing significant challenges in terms of both therapeutic intervention and prognostic evaluation. Given that the liver is the largest metabolic organ, a prognostic risk model grounded in single-cell transcriptome analysis and a metabolic perspective can facilitate precise prevention and treatment strategies for liver cancer. Hence, we identified 11 cell types in a scRNA-seq profile comprising 105,829 cells and found that the metabolic activity of malignant cells increased significantly. Subsequently, a prognostic risk model incorporating tumor heterogeneity, cell interactions, tumor cell metabolism, and differentially expressed genes was established based on eight genes; this model can accurately distinguish the survival outcomes of liver cancer patients and predict the response to immunotherapy. Analyzing the immune status and drug sensitivity of the high- and low-risk groups identified by the model revealed that the high-risk group had more active immune cell status and greater expression of immune checkpoints, indicating potential risks associated with liver cancer-targeted drugs. In summary, this study provides direct evidence for the stratification and precise treatment of liver cancer patients, and is an important step in establishing reliable predictors of treatment efficacy in liver cancer patients.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2073-4425/15/6/755; https://doaj.org/toc/2073-4425

URL الوصول: https://doaj.org/article/1a4a52b331314546b596e4e50269b705

المؤلفون: Yuetao Han, Peiyue Zhao, Yuan Zhao, Min Liu, Erhu Guo, Guoliang Wang, Aiying Zhang

المصدر: Agronomy, Vol 14, Iss 6, p 1114 (2024)

مصطلحات موضوعية: foxtail millet, grain filling rate, transcriptome, metabolome, auxin, Agriculture

الوصف: The grain filling rate (GFR) plays a crucial role in determining grain yield. However, the regulatory and molecular mechanisms of the grain filling rate (GFR) in foxtail millet remains unclear. In this study, we found that the GFR of ′Changnong No.47′ (CN47) was significantly higher at 14 DAF (days after flowering) and 21 DAF in comparison to ‘Changsheng 13’ (CS13). Furthermore, CN47 also exhibited higher a thousand-grain weight and yield than CS13. Therefore, RNA-seq and UHPLC-MS/MS were used to conduct transcriptome and metabolome analyses during two stages of grain filling in both cultivars. Conjoint analysis of transcriptomics and metabolomics was adopted in order to analyze the biological processes and functional genes associated with GFR. The results identified a total of 765 differentially expressed genes (DEGs) and 246 differentially accumulated metabolites (DAMs) at the 14 DAF stage, while at the 21 DAF stage, a total of 908 DEGs and 268 DAMs were identified. The integrated analysis of co-mapped DAMs and DEGs revealed enriched pathways, including flavonoid biosynthesis, plant hormone signal transduction, tyrosine metabolism, ATP-binding cassette (ABC) transporters, and beta-Alanine metabolism, as well as stilbenoid, diarylheptanoid, and gingerol biosynthesis. In order to elucidate their potential functions in the context of GFR, we developed a gene–metabolite regulatory network for these metabolic pathways. Notably, we found that some genes associated with ABC transporters and the plant hormone signal transduction pathway were implicated in auxin transport and signal transduction, highlighting the crucial role of auxin during grain filling. These findings provide initial insights into the regulatory and molecular mechanisms underlying GFR in foxtail millet, as well as offering valuable genetic resources for further elucidation of GFR in future studies. The findings have also established a theoretical basis for improving the efficiency of yield breeding in foxtail millet.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2073-4395/14/6/1114; https://doaj.org/toc/2073-4395

URL الوصول: https://doaj.org/article/514f796c947f4f4a91e377839ec5a8e3

المؤلفون: Kewei Chen, Zhuo Liu, Yuxuan Li, Xun Zhao, Guoliang Wang, Xiaojun Tian, Hongxian Zhang, Lulin Ma, Shudong Zhang

المصدر: Cancer Medicine, Vol 13, Iss 1, Pp n/a-n/a (2024)

مصطلحات موضوعية: chyle leak, radical nephrectomy, renal cell carcinoma, tumor thrombus, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background To define the incidence and risk factors of chyle leak (CL) after radical nephrectomy and thrombectomy and to determine the impact of chyle leak on oncological outcomes. Patients and Methods A total of 445 patients who underwent radical nephrectomy and thrombectomy between January 2014 and January 2023 were included. CL is defined as the drainage of chyle with a triglyceride level greater than 110 mg/dL after oral intake or enteral nutrition. Multivariate logistic regression analysis was performed to identify the risk factors of postoperative (CL). The Kaplan–Meier curves were used to compare overall survival and cancer‐specific survival. Results 44 patients (9.9%) were diagnosed as (CL). All patients developed CL within 6 days after the operation with a median time of 3 days. In multivariate logistic regression analysis, Mayo grade and side were independent patient‐related risk factors. In addition, operation approach, operation time, and number of lymph nodes harvested were independent surgery‐related risk factors. Between the CL group and the non‐CL group, neither overall survival nor cancer‐specific survival showed statistical differences. Conclusion Based on this retrospective study of renal cell carcinoma and tumor thrombus patients in our center, we found that the risk factors were Mayo grade, side, operation approach, operation time, and number of lymph nodes harvested, and the occurrence of CL significantly prolonged hospital stay, but had no effect on long‐term oncological outcomes.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2045-7634

URL الوصول: https://doaj.org/article/49aa9557de944181bc760da302ae8eff

المؤلفون: Shilong Lei, Xiangtao Wang, Jie Wang, Lu Zhang, Lirong Liao, Guobin Liu, Guoliang Wang, Zilin Song, Chao Zhang

المصدر: mSystems, Vol 9, Iss 1 (2024)

مصطلحات موضوعية: diazotrophs, co-occurrence patterns, alpine ecosystem, climate changes, Microbiology, QR1-502

الوصف: ABSTRACTMicrobial diversity plays a vital role in the maintenance of ecosystem functions. However, the current understanding of mechanisms that shape microbial diversity along environmental gradients at broad spatial scales is relatively limited, especially for specific functional groups, such as potential diazotrophs. Here, we conducted an aridity-gradient transect survey from 60 sites across the Tibetan Plateau, the largest alpine ecosystem of the planet, to investigate the ecological processes (e.g., local species pools, community assembly processes, and co-occurrence patterns) that underlie the β-diversity of alpine soil potential diazotrophic communities. We found that aridity strongly and negatively affected the abundance, richness, and β-diversity of soil diazotrophs. Diazotrophs displayed a distance-decay pattern along the aridity gradient, with organisms living in lower aridity habitats having a stronger distance-decay pattern. Arid habitats had lower co-occurrence complexity, including the number of edges and vertices, the average degree, and the number of keystone taxa, as compared with humid habitats. Local species pools explained limited variations in potential diazotrophic β-diversity. In contrast, co-occurrence patterns and stochastic processes (e.g., dispersal limitation and ecological drift) played a significant role in regulating potential diazotrophic β-diversity. The relative importance of stochastic processes and co-occurrence patterns changed with increasing aridity, with stochastic processes weakening whereas that of co-occurrence patterns enhancing. The genera Geobacter and Paenibacillus were identified as keystone taxa of co-occurrence patterns that are associated with β-diversity. In summary, aridity affects the co-occurrence patterns and community assembly by regulating soil and vegetation characteristics and ultimately shapes the β-diversity of potential diazotrophs. These findings highlight the importance of co-occurrence patterns in structuring microbial diversity and advance the current understanding of mechanisms that drive belowground communities.IMPORTANCERecent studies have shown that community assembly processes and species pools are the main drivers of β-diversity in grassland microbial communities. However, co-occurrence patterns can also drive β-diversity formation by influencing the dispersal and migration of species, the importance of which has not been reported in previous studies. Assessing the impact of co-occurrence patterns on β-diversity is important for understanding the mechanisms of diversity formation. Our study highlights the influence of microbial co-occurrence patterns on β-diversity and combines the drivers of community β-diversity with drought variation, revealing that drought indirectly affects β-diversity by influencing diazotrophic co-occurrence patterns and community assembly.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2379-5077

URL الوصول: https://doaj.org/article/9018c47f78944a1990f5f148438e1edc

المؤلفون: Guoliang Wang, Xinpeng Yan, Xi Yang, Luoluo Feng, Huiyan Pang, Runguang Zhang, Youlin Zhang

المصدر: Journal of Functional Foods, Vol 110, Iss , Pp 105877- (2023)

مصطلحات موضوعية: Walnut green husk, Polysaccharide, Structural characterization, Immunomodulatory activity, Nutrition. Foods and food supply, TX341-641

الوصف: Acidic walnut green husk polysaccharide with weight average molecular weight of 5.101 kDa was isolated and designated WGHP-2, which was composed of galacturonic acid (69.47 %), galactose (11.18 %), rhamnose (8.67 %), arabinose (3.96 %), glucose (2.21 %), glucuronic acid (2.28 %), xylose (0.83 %), fucose (0.81 %) and mannose (0.59 %). The main glycosidic linkage types of WGHP-2 were → 4-α-D-Glcp-(1→, →4-α-D-GalpA-(1→, t-β-D-GlcpA-(1→, →2-β-D-Rhap-(1→, t-α-D-Galp-(1→, →4-α-D-Galp-(1 → and → 2,4)-α-D-Manp(1 →. Immunostimulating assays showed that WGHP-2 could significantly promote the proliferation of RAW264.7 cells, strengthen the phagocytic activity, and up-regulate the production and release of NO, ROS, TNF-α, INF-β and IL-6. Western-blot analysis indicated that MAPK and PI3K/Akt pathways were involved in the activation of macrophages induced by WGHP-2. Overall, our study suggests that WGHP-2 has the potential to be developed as a functional food with good immunostimulatory activity.

وصف الملف: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S1756464623004772; https://doaj.org/toc/1756-4646

URL الوصول: https://doaj.org/article/acf06de39fc742e2839ce5ffbabe4555

المؤلفون: Guoliang Wang, Xiuyu Liang

المصدر: 地质科技通报, Vol 42, Iss 4, Pp 201-209 (2023)

مصطلحات موضوعية: baseflow recession, aquifer recharge, river stage fluctuation, riverbank permeability, analytical solution, Geology, QE1-996.5, Engineering geology. Rock mechanics. Soil mechanics. Underground construction, TA703-712

الوصف: Objective Baseflow recession analysis is an effective approach to estimating watershed-scale hydrogeological parameters. However, the traditional baseflow recession model did not consider the effects of semipervious riverbanks, and their influence on parameter estimation is unclear. Methods To address this issue, a mathematical model for groundwater flow in an unconfined aquifer with time-dependent recharge and river stages is presented. The effects of the semipervious riverbank are specifically taken into consideration. The analytical solutions of the hydraulic head and discharge are derived by using Green's function method, and their validities are tested by numerical simulations. Results The results show that, forced by the fluctuating recharge rate, the lower riverbank permeability leads to a higher peak of hydraulic heads, a lower baseflow, and slower baseflow recessions. For the case forced by the fluctuating river stages, the lower riverbank permeability leads to the weaker responses of water flow to the fluctuated river stage and the lower fluxes of surface water-groundwater interaction. The riverbank permeability significantly affects the baseflow recession curves. During early stage, the low riverbank permeability caused the power index of the recession curve to be larger than 3. For later stage, the power index approaches 1, which is not affected by the riverbank permeability. Conclusion For a low riverbank permeability, the traditional model will overestimate the baseflow and underestimate the hydraulic conductivities of aquifers because it neglects the effects of riverbank permeability.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2096-8523

URL الوصول: https://doaj.org/article/bc3903c9ef4d4ec6a8edad470547ca95