المؤلفون: Valter Paes de Almeida, Irailson Thierry Monchak, João Vitor da Costa Batista, Mirio Grazi, Hartmut Ramm, Vijayasankar Raman, Stephan Baumgartner, Carla Holandino, Jane Manfron

المصدر: Scientific Reports, Vol 13, Iss 1, Pp 1-13 (2023)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract Viscum album L. (Santalaceae) is an important medicinal plant traditionally used to treat several diseases, including cancer therapy. This paper provides detailed morpho-anatomical characteristics of the leaves, stems and berries of Viscum album subsp. album growing as hemi-parasite on the branches of Malus domestica (Suckow) Borkh. (Rosaceae) to aid species identification and botanical characterization. Additionally, for the first time, microchemical analyses of all tissues and Energy Dispersive X-Ray Spectroscopy analyses of the calcium oxalate crystals are provided for the first time. The plant features leathery presents green leaves with parallel veins, small yellow unisexual flowers in 3-flowered cymes, and the dioecious inflorescences usually consist of three flowers, with female flowers generating white fleshy berries, in which a seed is embedded in the mucilaginous mesocarp, normally containing two embryos. Anatomically, the analyzed leaves were isobilateral and amphistomatic, and showed straight anticlinal epidermal cell walls, thick cuticles with epicuticular wax crystalloids, and paracytic stomata. The midrib is flat on both sides and has a single vascular bundle, whereas the strongly shortened petiole is concave-convex in shape and contains five bundles. The stems show a primary structure with a ring of nine vascular bundles enclosing the pith. Calcium oxalate druses and cubic and quadrangular prisms were observed in different plant parts. The results of this study provide new microscopy information that can help in the authentication of mistletoe raw materials.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2045-2322

URL الوصول: https://doaj.org/article/65440763c9b548839c035f2c2b741b35

المؤلفون: Carla Holandino, Michelle Nonato de Oliveira Melo, Adriana Passos Oliveira, João Vitor da Costa Batista, Marcia Alves Marques Capella, Rafael Garrett, Mirio Grazi, Hartmut Ramm, Claudia Dalla Torre, Gerhard Schaller, Konrad Urech, Ulrike Weissenstein, Stephan Baumgartner

المصدر: BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-11 (2020)

مصطلحات موضوعية: Mistletoe, Viscum album L., Phytochemistry, Anti-proliferative activity, Cytotoxicity, Other systems of medicine, RZ201-999

الوصف: Abstract Background Viscum album L. (Santalaceae), commonly known as mistletoe, is a hemiparasitic plant traditionally used in complementary cancer treatment. Its antitumor potential is mostly attributed to the presence of aqueous soluble metabolites; however, the use of ethanol as solvent also permits the extraction of pharmacological compounds with antitumor potential. The clinical efficacy of mistletoe therapy inspired the present work, which focuses on ethanolic extracts (V. album “mother tinctures”, MT) prepared from different host trees. Methods Samples from three European subspecies (album, austriacum, and abietis) were harvested, and five different V. album-MT strains were prepared. The following phytochemical analyses were performed: thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) and liquid chromatography-high resolution mass spectrometry (LC-HRMS). The proliferation assay was performed with WST-1 after incubation of tumor (Yoshida and Molt-4) and fibroblast cell lines (NIH/3 T3) with different MT concentrations (0.5 to 0.05% v/v). The cell death mechanism was investigated by flow cytometry (FACS) using Annexin V-7AAD. Results Chemical analyses of MT showed the presence of phenolic acids, flavonoids and lignans. The MT flavonoid and viscotoxin contents (mg/g fresh weight) were highest in Quercus robur (9.67 ± 0.85 mg/g) and Malus domestica (3.95 ± 0.58 mg/mg), respectively. The viscotoxin isoform proportions (% total) were also different among the VA subspecies with a higher content of A3 in V. album growing on Abies alba (60.57 ± 2.13). The phytochemical compounds as well as the viscotoxin contents are probably related to the antitumor effects of MT. The cell death mechanisms evaluated by colorimetric and FACS methodologies involved necrotic damage, which was host tree-, time- and dose- dependent, with different selectivity to tumor cells. Mother tincture from V. album ssp. abietis was the most effective at inducing in vitro cellular effects, even when incubated at the smallest concentration tested, probably because of the higher content of VT A3. Conclusion Our results indicate the promising antitumor potential of Viscum album ethanolic extracts and the importance of botanical and phytochemical characterization for in vitro anti-proliferative effects.

وصف الملف: electronic resource

Relation: http://link.springer.com/article/10.1186/s12906-020-02987-4; https://doaj.org/toc/2662-7671

URL الوصول: https://doaj.org/article/f6fb059bda8242f2b4c405cb03aa8e1b

المؤلفون: Tim Jäger, Carla Holandino, Michelle Nonato de Oliveira Melo, Evelyn Maribel Condori Peñaloza, Adriana Passos Oliveira, Rafael Garrett, Gaétan Glauser, Mirio Grazi, Hartmut Ramm, Konrad Urech, Stephan Baumgartner

المصدر: Plants, Vol 10, Iss 8, p 1726 (2021)

مصطلحات موضوعية: Viscum album, mistletoe, host tree, metabolite fingerprinting, multivariate analysis, Botany, QK1-989

الوصف: Viscum album L., commonly known as European mistletoe, is a hemi-parasitic plant of the Santalaceae family. The in vitro and in vivo effects of V. album differ, according to its host tree. However, little is known about the host-dependent phytochemical diversity in V. album. In this study, the metabolic profiles of V. album ssp. album from Malus domestica Bork., Quercus robur L., and Ulmus carpinifolia Gled were compared. Leaves, stems, and berries were collected in Switzerland, by the same procedure, in September 2016 and 2017. The methanolic extracts were analyzed by ultra-performance liquid chromatography, coupled to electrospray quadrupole time-of-flight mass spectrometry in positive ionization mode. The data were submitted to partial-least square discriminant analysis (PLS-DA) and the results showed that the V. album ssp. album samples were clustered into three groups, according to the three distinct host trees. Seven compounds, with high VIP scores (variable importance in projection), were responsible for this differentiation. The following four compounds were detected in both the harvest years: arginine, pipecolic acid or lysine, dimethoxycoumarin, and sinapyl alcohol, suggesting their use as host specific V. album biomarkers. The present work highlights the importance of standardized harvest and analytical procedures for the reproducibility of the chemical results of herbal materials.

وصف الملف: electronic resource

Relation: https://www.mdpi.com/2223-7747/10/8/1726; https://doaj.org/toc/2223-7747

URL الوصول: https://doaj.org/article/318fbb0421354f3dbe280e8c2d97d9da

المؤلفون: Valter Paes de Almeida, Irailson Thierry Monchak, João Vitor da Costa Batista, Mirio Grazi, Hartmut Ramm, Vijayasankar Raman, Stephan Baumgartner, Carla Holandino, Jane Manfron

الوصف: This paper provides detailed morpho-anatomical characteristics of the leaves, stems and berries of Viscum album L. subsp. album (Santalaceae) growing on the branches of Malus domestica. Additionally, microchemical analyses of all tissues and Energy Dispersive X-Ray Spectroscopy (EDS) analyses of the calcium oxalate crystals are provided for the first time. The plant grows as a semi-parasite on the branches of host trees and shrubs; it develops as dichasium with pseudo-dichotomous branching stems and well-developed green leaves with parallel veins; the dioecious inflorescences usually consist of three flowers, with female flowers generating white fleshy berries, in which a seed is embedded in the mucilaginous mesocarp, normally containing two embryos. The analyzed leaves were isobilateral, amphistomatic, and showed straight anticlinal epidermal cell walls, thick cuticles with epicuticular wax crystalloids, and paracytic stomata. The midrib is flat on both sides and has a single vascular bundle, whereas the petiole is concave-convex in shape and contains five bundles. The stems show a primary structure with a ring of nine vascular bundles enclosing the pith. Calcium oxalate druses and cubic and quadrangular prisms were observed in different parts of the plant. The microscopic features of the tissues and structures are illustrated with light and scanning electron micrographs.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::361b82af0dd19e53077ed4a603a9a599
https://doi.org/10.21203/rs.3.rs-2358286/v1

المؤلفون: G. Schaller, Stephan Baumgartner, Hartmut Ramm, K. Urech

المصدر: Der Merkurstab. 74:219-226

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::d849aafee0b52d5ea51544c6dcf99f5d
https://doi.org/10.14271/dms-21357-de

المؤلفون: Michelle Nonato de Oliveira Melo, Adriana Passos Oliveira, Rafael Garrett, Patrícia Zancan, Alan Clavelland Ochioni, Mirio Grazi, Hartmut Ramm, Tim Jaeger, Stephan Baumgartner, Carla Holandino

المصدر: International Journal of High Dilution Research - ISSN 1982-6206. 21:33-33

مصطلحات موضوعية: Complementary and alternative medicine

الوصف: Background: Viscum album L. is a semi-parasitic plant with antitumor activity attributed to the aqueous extracts. However, European V. album ethanolic extracts (VAE) have also demonstrated in vitro activity in tumor models. Aims: Evaluate the metabolic profiles of fifty VAE harvested during summer and winter seasons and their antitumor activity through 2D and 3D models. Methodology: VAE were prepared by maceration from: V. album subsp. album growing on Malus domestica, Quercus sp. and Ulmus sp.; V. album subsp. austriacum from Pinus sylvestris; V. album subsp. abietis from Abies alba. Chemical analyses were performed through liquid chromatography coupled with high resolution mass spectrometry and Partial Least Squares Discriminant Analysis (PLS-DA) was performed in the Metaboanalyst 4.0. The antitumor potential of the selected VAE was evaluated in 2D and 3D models (MDA-MB-231 cancer cells) by MTT, crystal violet and glycolytic pathway analysis. Results and discussion: The first 3 principal components in PLS-DA explained 60% and 40% of data variation in positive and negative modes respectively. Three groups were formed and showed chemical similarity among V. album subspecies. The compounds responsible for group separation were tentatively identified as: pinobankasin or naringenin hexoside; isorhamnetin-3-hexoside, meglutol and different amino acids. The summer VAE at 0.5% v/v induced higher cytotoxic damage than the winter preparations, and Abies alba and Quercus sp. VAE promoted 49% and 42% reduction of tumor viability in 3D model (72h incubation), respectively. MDA-MB-231 glycolytic pathway in 2D model showed a decrease in the glucose consumption and extracellular lactate production. Also, PFK (6- phosphofructo-1-kinase) and PK (Pyruvate kinase) activities were inhibited by Abies alba and Quercus sp. VAE at 48h of incubation. Conclusion: VAE extracts showed different metabolomes and the glycolytic pathway should be an important target involved in the inhibition of tumor growth by these extracts.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::a422e3428c7e9b9dbcfdb5a6664aa6f9
https://doi.org/10.51910/ijhdr.v21i1.1195

المؤلفون: Adriana Passos Oliveira, Mirio Grazi, Stephan Baumgartner, Tim Jäger, Konrad Urech, Carla Holandino, Gaétan Glauser, Hartmut Ramm, Evelyn Maribel Condori Peñaloza, Rafael Garrett, Michelle Nonato de Oliveira Melo

المصدر: Plants, Vol 10, Iss 1726, p 1726 (2021)
Jäger, Tim; Holandino, Carla; Melo, Michelle Nonato de Oliveira; Peñaloza, Evelyn Maribel Condori; Oliveira, Adriana Passos; Garrett, Rafael; Glauser, Gaétan; Grazi, Mirio; Ramm, Hartmut; Urech, Konrad; Baumgartner, Stephan (2021). Metabolomics by UHPLC-Q-TOF Reveals Host Tree-Dependent Phytochemical Variation in Viscum album L. Plants, 10(8) MDPI 10.3390/plants10081726 <http://dx.doi.org/10.3390/plants10081726>
Plants
Volume 10
Issue 8

مصطلحات موضوعية: Malus, Ecology, biology, Santalaceae, Traditional medicine, Host (biology), Botany, 610 Medicine & health, metabolite fingerprinting, Plant Science, biology.organism_classification, Quercus robur, chemistry.chemical_compound, Metabolomics, multivariate analysis, Sinapyl alcohol, chemistry, Phytochemical, QK1-989, host tree, Viscum album, mistletoe, Ecology, Evolution, Behavior and Systematics

الوصف: Viscum album L., commonly known as European mistletoe, is a hemi-parasitic plant of the Santalaceae family. The in vitro and in vivo effects of V. album differ, according to its host tree. However, little is known about the host-dependent phytochemical diversity in V. album. In this study, the metabolic profiles of V. album ssp. album from Malus domestica Bork., Quercus robur L., and Ulmus carpinifolia Gled were compared. Leaves, stems, and berries were collected in Switzerland, by the same procedure, in September 2016 and 2017. The methanolic extracts were analyzed by ultra-performance liquid chromatography, coupled to electrospray quadrupole time-of-flight mass spectrometry in positive ionization mode. The data were submitted to partial-least square discriminant analysis (PLS-DA) and the results showed that the V. album ssp. album samples were clustered into three groups, according to the three distinct host trees. Seven compounds, with high VIP scores (variable importance in projection), were responsible for this differentiation. The following four compounds were detected in both the harvest years: arginine, pipecolic acid or lysine, dimethoxycoumarin, and sinapyl alcohol, suggesting their use as host specific V. album biomarkers. The present work highlights the importance of standardized harvest and analytical procedures for the reproducibility of the chemical results of herbal materials.

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c72d18ffe3a99780b5cedb19eb894db1

المؤلفون: Ulrike Weissenstein, Leandro Rocha, Matthias Kunz, João Vc Batista, Daniela S. Alviano, Fortune Homsani, Celuta S. Alviano, Adriana Passos Oliveira, Mirio Grazi, Venicio Feo da Veiga, Michelle No Melo, Carla Holandino Quaresma, Hartmut Ramm, Konrad Urech, Stephan Baumgartner

المصدر: International Journal of High Dilution Research - ISSN 1982-6206. 17:25-26

مصطلحات موضوعية: Minimum inhibitory concentration, Malus, Complementary and alternative medicine, Phytochemical, Traditional medicine, biology, Chemistry, Viscum album, Biological activity, Antimicrobial, biology.organism_classification, Incubation, In vitro

الوصف: Several in vitro and in vivo studies investigated the biological properties of mistletoe preparations [1-4]. In Europe, where phytochemical constituents of aqueous Viscum album extracts were previously described, the most widespread and common form of mistletoe is the white-berried species (Viscum album) [5]. The aim of the present study was to analyze the biological activity and phytochemical features of different Viscum album ssp mother tinctures collected in 2 different harvesting periods. Leaves, stems and berries of Viscum album samples were subjected to ethanolic extraction (45% v/v) following the homeopathic pharmacopeia [6,7]. The mother tinctures (MT) prepared were: V. album (from 3 host trees Malus domestica, Quercus sp, Ulmus sp), V. album ssp. austriacum (host tree Pinus sylvestris) and V. album ssp. abietis (host tree Abies pectinata). All samples were collected at Höfli farm (Switzerland) in July and August and subjected to chemical analysis by means of high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) and Thin-Layer Chromatography (TLC). Tumor (Yoshida and Molt4) and non-tumor cells (Ma104) were incubated with MT (1; 0.1; 0.01%) for 4, 24 and 48 hours at 37ºC. Proliferation was indirectly measured using WST-1 (tumor cells) and MTT (non-tumor cells). All cells (1.5 x 104/100 μL) were plated in triplicate in 96-well plates. The cell proliferation inhibition rate was calculated as percent inhibition relative to control cells treated with the MT solvent (45% ethanol v/v). Apoptosis/necrosis was measured using 2 x 105 cells incubated with Annexin V-FITC and 7-AAD at room temperature in the dark, and analyzed in flow cytometer [2]. Antimicrobial activity was assessed for Candida albicans, Cryptococcus neoformans, Escherichia coli and Staphylococcus aureus following the Clinical Laboratory Standards Institute manuals [8-9] and compared with 45% ethanol v/v (MT solvent). The chromatographic assays of MT samples showed phenolic acids and lignans as main chemical classes identified. WST-1 and MTT experiments revealed different antitumor potential of MT. In general, Yoshida cells were more sensitive than Molt4. The maximal cytotoxic effect with 100% of mortality was attained by incubation of Yoshida and Molt-4 cells with 1% MT of V. album ssp. abietis (harvested in July) for 4 hours. Preliminary results with non-tumor cells indicate lower cytotoxicity compared to tumor cells. Annexin V/7ADD showed predominance of late apoptotic/necrotic events after 4 and 24 hours of incubation with all MT. The antimicrobial assay showed that MT of Viscum album ssp. austriacum (host tree Pinus sylvestris) exhibited minimum inhibitory concentration 7.25 mg/mL for the 2 tested bacterial strains. The results of the present study suggest that Viscum album samples prepared according to the homeopathic pharmacopeia exhibit promising biological activities in a dose-dependent manner. The effects of potentiation will be assessed and compared to non-potentiated samples in future experiments. Therefore, the present study contributes with data on the main chemical components in homeopathic preparations of Viscum album, drawing attention to the relevance of extract solvent, seasonal aspects, host tree and harvested parts of plant harvested to the quality of thee mother tinctures.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::09edc7b2aa039371a8d46828d58b9674
https://doi.org/10.51910/ijhdr.v17i1.900

المؤلفون: Gerhard Schaller, Adriana Passos Oliveira, Rafael Garrett, Konrad Urech, Carla Holandino, Ulrike Weissenstein, Mirio Grazi, Claudia Dalla Torre, Marcia A. M. Capella, Stephan Baumgartner, João Vitor da Costa Batista, Hartmut Ramm, Michelle Nonato de Oliveira Melo

المصدر: BMC Complementary Medicine and Therapies
BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-11 (2020)

مصطلحات موضوعية: Phytochemistry, Viscum album, Cytotoxicity, Flavonoid, Antineoplastic Agents, Apoptosis, In Vitro Techniques, 01 natural sciences, High-performance liquid chromatography, 03 medical and health sciences, Cell Line, Tumor, Viscotoxins, Animals, Humans, Viscum album L, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, biology, Santalaceae, Traditional medicine, Plant Extracts, 010405 organic chemistry, Tincture, lcsh:Other systems of medicine, lcsh:RZ201-999, biology.organism_classification, Mistletoe, 0104 chemical sciences, Complementary and alternative medicine, chemistry, Phytochemical, Anti-proliferative activity, Research Article

الوصف: Background Viscum album L. (Santalaceae), commonly known as mistletoe, is a hemiparasitic plant traditionally used in complementary cancer treatment. Its antitumor potential is mostly attributed to the presence of aqueous soluble metabolites; however, the use of ethanol as solvent also permits the extraction of pharmacological compounds with antitumor potential. The clinical efficacy of mistletoe therapy inspired the present work, which focuses on ethanolic extracts (V. album “mother tinctures”, MT) prepared from different host trees. Methods Samples from three European subspecies (album, austriacum, and abietis) were harvested, and five different V. album-MT strains were prepared. The following phytochemical analyses were performed: thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) and liquid chromatography-high resolution mass spectrometry (LC-HRMS). The proliferation assay was performed with WST-1 after incubation of tumor (Yoshida and Molt-4) and fibroblast cell lines (NIH/3 T3) with different MT concentrations (0.5 to 0.05% v/v). The cell death mechanism was investigated by flow cytometry (FACS) using Annexin V-7AAD. Results Chemical analyses of MT showed the presence of phenolic acids, flavonoids and lignans. The MT flavonoid and viscotoxin contents (mg/g fresh weight) were highest in Quercus robur (9.67 ± 0.85 mg/g) and Malus domestica (3.95 ± 0.58 mg/mg), respectively. The viscotoxin isoform proportions (% total) were also different among the VA subspecies with a higher content of A3 in V. album growing on Abies alba (60.57 ± 2.13). The phytochemical compounds as well as the viscotoxin contents are probably related to the antitumor effects of MT. The cell death mechanisms evaluated by colorimetric and FACS methodologies involved necrotic damage, which was host tree-, time- and dose- dependent, with different selectivity to tumor cells. Mother tincture from V. album ssp. abietis was the most effective at inducing in vitro cellular effects, even when incubated at the smallest concentration tested, probably because of the higher content of VT A3. Conclusion Our results indicate the promising antitumor potential of Viscum album ethanolic extracts and the importance of botanical and phytochemical characterization for in vitro anti-proliferative effects.

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::407c24e38883de7ae8720392f6e1212b
https://doi.org/10.1186/s12906-020-02987-4

المؤلفون: Adriana Passos Oliveira, Rafael Garrett, Carla Holandino, Michelle Nonato de Oliveira Melo, Hartmut Ramm, Tim Jäger, Stephan Baumgartner, Gaétan Glauser, Mirio Grazi

المصدر: Phytomedicine. 61:1-2

مصطلحات موضوعية: Pharmacology, Complementary and alternative medicine, biology, Drug Discovery, Botany, Viscum album, Pharmaceutical Science, Molecular Medicine, biology.organism_classification

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::8391219f6d84d69912c888036c51fd86
https://doi.org/10.1016/j.phymed.2019.09.119