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1دورية أكاديمية
المؤلفون: Brent Delzer, Dawei Liang, David Szwerdszarf, Isadora Rodriguez, Gonzalo Mardones, Sivamani Elumalai, Francine Johnson, Samson Nalapalli, Rachel Egger, Erin Burch, Kerry Meier, Juan Wei, Xiujuan Zhang, Huaping Gui, Huaibing Jin, Huan Guo, Kun Yu, Yubo Liu, Becky Breitinger, Ana Poets, Jason Nichols, Wan Shi, David Skibbe, Qiudeng Que, Timothy Kelliher
المصدر: Crop Journal, Vol 12, Iss 1, Pp 314-319 (2024)
مصطلحات موضوعية: Zea mays L., Doubled haploids, Transformation, Genome editing, QTL, Agriculture, Agriculture (General), S1-972
الوصف: The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly. Two technologies that are disrupting traditional breeding processes are doubled haploid (DH) breeding and genome editing (GE). Recently, these techniques were combined into a GE trait delivery system called HI-Edit (Haploid Inducer-Edit) [1]. In HI-Edit, the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety, obviating recurrent selection. For HI-Edit to operate at scale, an efficient transformable HI line is needed, but most maize varieties are recalcitrant to transformation, and haploid inducers are especially difficult to transform given their aberrant reproductive behaviors. Leveraging marker assisted selection and a three-tiered testing scheme, we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable, have high haploid induction rates, and exhibit a robust, genetically-dominant anthocyanin native trait that may be used for rapid haploid identification. We show that transformation of these elite “HI-Edit” lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic factors. Finally, we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk testers. The strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Mengjie Zhu, Nan Wu, Jiayi Zhong, Chen Chen, Wenwen Liu, Yingdang Ren, Xifeng Wang, Huaibing Jin
المصدر: Cell Reports, Vol 43, Iss 2, Pp 113821- (2024)
مصطلحات موضوعية: CP: Microbiology, Biology (General), QH301-705.5
الوصف: Summary: The titer of viruses that persist and propagate in their insect vector must be high enough for transmission yet not harm the insect, but the mechanism of this dynamic balance is unclear. Here, expression of inosine monophosphate dehydrogenase (LsIMPDH), a rate-limiting enzyme for guanosine triphosphate (GTP) synthesis, is shown to be downregulated by increased levels of N6-methyladenosine (m6A) on LsIMPDH mRNA in rice stripe virus (RSV)-infected small brown planthoppers (SBPHs; Laodelphax striatellus), the RSV vector, which decreases GTP content, thus limiting viral proliferation. Moreover, planthopper methyltransferase-like protein 3 (LsMETTL3) and m6A reader protein LsYTHDF3 are found to catalyze and recognize the m6A on LsIMPDH mRNA, respectively, and cooperate in destabilizing LsIMPDH transcripts. Co-silencing assays show that negative regulation of viral proliferation by both LsMETTL3 and LsYTHDF3 is partially dependent on LsIMPDH. This distinct mechanism limits virus replication in an insect vector, providing a potential gene target to block viral transmission.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Jibin Xiao, Lingli Dong, Huaibing Jin, Juncheng Zhang, Kunpu Zhang, Na Liu, Xinyun Han, Hongyuan Zheng, Wenming Zheng, Daowen Wang
المصدر: Crop Journal, Vol 6, Iss 5, Pp 509-515 (2018)
مصطلحات موضوعية: Agriculture, Agriculture (General), S1-972
الوصف: Triticum urartu (AA, 2n = 2x = 14), a wild grass endemic to the Fertile Crescent (FC), is the progenitor of the A subgenome in common wheat. It belongs to the primary gene pool for wheat improvement. Here, we evaluated the yellow rust (caused by Puccinia striiformis f. sp. tritici, Pst) reactions of 147 T. urartu accessions collected from different parts of the FC. The reactions varied from susceptibility to strong resistance. In general, there were more accessions with stronger resistance to race CYR33 than to CYR 32. In most cases the main form of defense was a moderate resistance characterized by the presence of necrotic/chlorotic lesions with fewer Pst uredinia on the leaves. Forty two accessions displayed resistance to both races. Histological analysis showed that Pst growth was abundant in the compatible interaction but significantly suppressed by the resistant response. Gene silencing mediated by Barley stripe mosaic virus was effective in two T. urartu accessions with different resistance responses, indicating that this method can expedite future functional analysis of resistance genes. Our data suggest that T. urartu is a valuable source of resistance to yellow rust, and represents a model for studying the genetic, genomic and molecular basis underlying interaction between wheat and Pst. Keywords: Common wheat, Disease resistance, Gene silencing, Puccinia striiformis
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Jiashu Guo, Peipei Zhang, Nan Wu, Wenwen Liu, Yan Liu, Huaibing Jin, Francis, Frederic, Xifeng Wang
المصدر: Proceedings of the National Academy of Sciences of the United States of America; 6/25/2024, Vol. 121 Issue 26, p1-10, 26p
مصطلحات موضوعية: METARHIZIUM, LAODELPHAX striatellus, PATHOGENIC fungi, METARHIZIUM anisopliae, FALL armyworm
مستخلص: Although most known viruses infecting fungi pathogenic to higher eukaryotes are asymptomatic or reduce the virulence of their host fungi, those that confer hypervirulence to entomopathogenic fungus still need to be explored. Here, we identified and studied a novel mycovirus in Metarhizium flavoviride, isolated from small brown planthopper (Laodelphax striatellus). Based on molecular analysis, we tentatively designated the mycovirus as Metarhizium flavoviride partitivirus 1 (MfPV1), a species in genus Gammapartitivirus, family Partitiviridae. MfPV1 has two double-stranded RNAs as its genome, 1,775 and 1,575 bp in size respectively, encapsidated in isometric particles. When we transfected commercial strains of Metarhizium anisopliae and Metarhizium pingshaense with MfPV1, conidiation was significantly enhanced (t test; P-value < 0. 01), and the significantly higher mortality rates of the larvae of diamondback moth (Plutella xylostella) and fall armyworm (Spodoptera frugiperda), two important lepidopteran pests were found in virus-transfected strains (ANOVA; P-value < 0.05). Transcriptomic analysis showed that transcript levels of pathogenesis-related genes in MfPV1-infected M. anisopliae were obviously altered, suggesting increased production of metarhizium adhesin-like protein, hydrolyzed protein, and destruxin synthetase. Further studies are required to elucidate the mechanism whereby MfPV1 enhances the expression ofpathogenesis-related genes and virulence ofMetarhizium to lepidopteran pests. This study presents experimental evidence that the transfection of other entomopathogenic fungal species with a mycovirus can confer significant hypervirulence and provides a good example that mycoviruses could be used as a synergistic agent to enhance the biocontrol activity of entomopathogenic fungi. [ABSTRACT FROM AUTHOR]
: Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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5دورية أكاديمية
المؤلفون: Delzer, Brent, Liang, Dawei, Szwerdszarf, David, Rodriguez, Isadora, Mardones, Gonzalo, Elumalai, Sivamani, Johnson, Francine, Nalapalli, Samson, Egger, Rachel, Burch, Erin, Meier, Kerry, Juan Wei, Xiujuan Zhang, Huaping Gui, Huaibing Jin, Huan Guo, Kun Yu, Yubo Liu, Breitinger, Becky, Poets, Ana
المصدر: Crop Journal (2095-5421); Feb2024, Vol. 12 Issue 1, p314-319, 6p
مصطلحات موضوعية: CORN varieties, HAPLOIDY, GERMPLASM of corn, ANTHOCYANINS, PLANT development, GENOME editing
مستخلص: The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly. Two technologies that are disrupting traditional breeding processes are doubled haploid (DH) breeding and genome editing (GE). Recently, these techniques were combined into a GE trait delivery system called HI-Edit (Haploid Inducer-Edit) [1]. In HI-Edit, the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety, obviating recurrent selection. For HI-Edit to operate at scale, an efficient transformable HI line is needed, but most maize varieties are recalcitrant to transformation, and haploid inducers are especially difficult to transform given their aberrant reproductive behaviors. Leveraging marker assisted selection and a three-tiered testing scheme, we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable, have high haploid induction rates, and exhibit a robust, genetically-dominant anthocyanin native trait that may be used for rapid haploid identification. We show that transformation of these elite ‘‘HI-Edit” lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic factors. Finally, we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk testers. The strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops. [ABSTRACT FROM AUTHOR]
: Copyright of Crop Journal (2095-5421) is the property of KeAi Communications Co. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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المؤلفون: Huaibing Jin, Daowen Wang, Xifeng Wang
المصدر: Trends in Plant Science. 28:512-514
مصطلحات موضوعية: Plant Science
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المؤلفون: Dawei Liang, Yubo Liu, Chao Li, Qin Wen, Jianping Xu, Lizhao Geng, Chunxia Liu, Huaibing Jin, Yang Gao, Heng Zhong, John Dawson, Bin Tian, Brenden Barco, Xiujuan Su, Shujie Dong, Changbao Li, Sivamani Elumalai, Qiudeng Que, Ian Jepson, Liang Shi
المصدر: Methods in Molecular Biology ISBN: 9781071631300
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::d2b99cbd29a2d9f38689a6881e85ac32
https://doi.org/10.1007/978-1-0716-3131-7_3 -
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المؤلفون: Huaibing Jin, Xinyun Han, Zhaohui Wang, Yilin Xie, Kunpu Zhang, Xiaoge Zhao, Lina Wang, Jin Yang, Huiyun Liu, Xiang Ji, Lingli Dong, Hongyuan Zheng, Weijuan Hu, Yan Liu, Xifeng Wang, Xueping Zhou, Yijing Zhang, Weiqiang Qian, Wenming Zheng, Qianhua Shen, Mingyue Gou, Daowen Wang
المصدر: The EMBO Journal. 41
مصطلحات موضوعية: Viral Proteins, Virulence, General Immunology and Microbiology, General Neuroscience, RNA, Viral, Hordeum, RNA Interference, RNA, Small Interfering, Antiviral Agents, Molecular Biology, General Biochemistry, Genetics and Molecular Biology, Plant Diseases
الوصف: Viruses often usurp host machineries for their amplification, but it remains unclear if hosts may subvert virus proteins to regulate viral proliferation. Here, we show that the 17K protein, an important virulence factor conserved in barley yellow dwarf viruses (BYDVs) and related poleroviruses, is phosphorylated by host GRIK1-SnRK1 kinases, with the phosphorylated 17K (P17K) capable of enhancing the abundance of virus-derived small interfering RNAs (vsiRNAs) and thus antiviral RNAi. Furthermore, P17K interacts with barley small RNA-degrading nuclease 1 (HvSDN1) and impedes HvSDN1-catalyzed vsiRNA degradation. Additionally, P17K weakens the HvSDN1-HvAGO1 interaction, thus hindering HvSDN1 from accessing and degrading HvAGO1-carried vsiRNAs. Importantly, transgenic expression of 17K phosphomimetics (17K
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المؤلفون: Guangwei Li, Yue Zhao, Yiwen Li, Huaibing Jin, Derong Gao, Daowen Wang, Wenjing Hu, Tao Peng, Shuyu Wu, Fei Lu, Jiangchun Wang, Huijie Zhai, Kunfang Yan, Kunpu Zhang, Xin Liu, Zhikai Jiang, Haitao Gao, Congcong Jiang, Guihong Yin, Xingqi Ou, Bingke Luo, Matthew P. Reynolds, Yanbing Zhang, Yi Du, Wenchen Qiao, Shuling Yang
المصدر: Plant Biotechnology Journal
مصطلحات موضوعية: Thermotolerance, 0106 biological sciences, 0301 basic medicine, Germplasm, Locus (genetics), Plant Science, Biology, 01 natural sciences, 03 medical and health sciences, heat stress tolerance, Gene mapping, wheat, TaHST1, Cultivar, Gene, Triticum, Research Articles, Genetics, gene deletion, Haplotype, Nucleic acid sequence, Chromosome Mapping, food and beverages, Plant Breeding, 030104 developmental biology, haplotype analysis, Chromosome Arm, Arm, genetic mapping, Agronomy and Crop Science, Research Article, 010606 plant biology & botany, Biotechnology
الوصف: Summary Heat stress (HS) causes substantial damages to worldwide crop production. As a cool season crop, wheat (Triticum aestivum) is sensitive to HS‐induced damages. To support the genetic improvement of wheat HS tolerance (HST), we conducted fine mapping of TaHST1, a locus required for maintaining wheat vegetative and reproductive growth under elevated temperatures. TaHST1 was mapped to the distal terminus of 4AL chromosome arm using genetic populations derived from two BC6F6 breeding lines showing tolerance (E6015‐4T) or sensitivity (E6015‐3S) to HS. The 4AL region carrying TaHST1 locus was approximately 0.949 Mbp and contained the last 19 high confidence genes of 4AL according to wheat reference genome sequence. Resequencing of E6015‐3S and E6015‐4T and haplotype analysis of 3087 worldwide wheat accessions revealed heightened deletion polymorphisms in the distal 0.949 Mbp region of 4AL, which was confirmed by the finding of frequent gene losses in this region in eight genome‐sequenced hexaploid wheat cultivars. The great majority (86.36%) of the 3087 lines displayed different degrees of nucleotide sequence deletions, with only 13.64% of them resembling E6015‐4T in this region. These deletions can impair the presence and/or function of TaHST1 and surrounding genes, thus rendering global wheat germplasm vulnerable to HS or other environmental adversities. Therefore, conscientious and urgent efforts are needed in global wheat breeding programmes to optimize the structure and function of 4AL distal terminus by ensuring the presence of TaHST1 and surrounding genes. The new information reported here will help to accelerate the ongoing global efforts in improving wheat HST.
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المؤلفون: Lanqin Xia, Huaibing Jin, Kunpu Zhang, Guangwei Li, Hongyuan Zheng, Daowen Wang, Xinyun Han, Zijun Xiong, Tengfei Xia, Xiang Ji, Yanping Yang, Weiqiang Qian
المصدر: Plant Biotechnology Journal
مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, TtdLRK10L‐1, receptor‐like kinase, intron, Blumeria graminis, Plant Science, Genetically modified crops, 01 natural sciences, 03 medical and health sciences, Ascomycota, Complementary DNA, wheat, MYB, Research Articles, Triticum, Disease Resistance, Plant Diseases, Genetics, Binding Sites, biology, fungi, food and beverages, biology.organism_classification, Introns, DNA binding site, genomic DNA, 030104 developmental biology, Ectopic expression, powdery mildew, Agronomy and Crop Science, Powdery mildew, 010606 plant biology & botany, Biotechnology, Research Article, MYB binding site
الوصف: Summary The LRK10‐like receptor kinases (LRK10L‐RLKs) are ubiquitously present in higher plants, but knowledge of their expression and function is still limited. Here, we report expression and functional analysis of TtdLRK10L‐1, a typical LRK10L‐RLK in durum wheat (Triticum turgidum L. ssp. durum). The introns of TtdLRK10L‐1 contained multiple kinds of predicted cis‐elements. To investigate the potential effect of these cis‐elements on TtdLRK10L‐1 expression and function, two types of transgenic wheat lines were prepared, which expressed a GFP‐tagged TtdLRK10L‐1 protein (TtdLRK10L‐1:GFP) from the cDNA or genomic DNA (gDNA) sequence of TtdLRK10L‐1 under the native promoter. TtdLRK10L‐1:GFP expression was up‐regulated by the powdery mildew pathogen Blumeria graminis f. sp. tritici (Bgt) in both types of transgenic plants, with the scale of the elevation being much stronger in the gDNA lines. Both types of transgenic plants exhibited enhanced resistance to Bgt infection relative to wild type control. Notably, the Bgt defence activated in the gDNA lines was significantly stronger than that in the cDNA lines. Further analysis revealed that a putative MYB transcription factor binding site (MYB‐BS, CAGTTA) located in TtdLRK10L‐1 intron I was critical for the efficient expression and function of TtdLRK10L‐1 in Bgt defence. This MYB‐BS could also increase the activity of a superpromoter widely used in ectopic gene expression studies in plants. Together, our results deepen the understanding of the expression and functional characteristics of LRK10L‐RLKs. TtdLRK10L‐1 is likely useful for further dissecting the molecular processes underlying wheat defence against Bgt and for developing Bgt resistant wheat crops.
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