المؤلفون: Cherry Kilbride, Daniel J M Scott, Tom Butcher, Meriel Norris, Alyson Warland, Karen Baker, Dimitrios A Athanasiou, Guillem Singla-Buxarrais, Jennifer Ryan, Elizabeth Cassidy

المصدر: BMJ Open, Vol 14, Iss 1 (2024)

مصطلحات موضوعية: Medicine

الوصف: Objective To report participants’ experiences of trial processes and use of the Neurofenix platform for home-based rehabilitation following stroke. The platform, consisting of the NeuroBall device and Neurofenix app, is a non-immersive virtual reality tool to facilitate upper limb rehabilitation following stroke. The platform has recently been evaluated and demonstrated to be safe and effective through a non-randomised feasibility trial (RHOMBUS).Design Qualitative approach using semistructured interviews. Interviews were audio recorded, transcribed verbatim and analysed using the framework method.Setting Participants’ homes, South-East England.Participants Purposeful sample of 18 adults (≥18 years), minimum 12 weeks following stroke, not receiving upper limb rehabilitation prior to the RHOMBUS trial, scoring 9–25 on the Motricity Index (elbow and shoulder), with sufficient cognitive and communicative abilities to participate.Results Five themes were developed which explored both trial processes and experiences of using the platform. Factors that influenced participant’s decision to take part in the trial, their perceptions of support provided during the trial and communication with the research team were found to be important contextual factors effecting participants’ overall experience. Specific themes around usability and comfort of the NeuroBall device, factors motivating persistence and perceived effectiveness of the intervention were highlighted as being central to the usability and acceptability of the platform.Conclusion This study demonstrated the overall acceptability of the platform and identified areas for enhancement which have since been implemented by Neurofenix. The findings add to the developing literature on the interface between virtual reality systems and user experience.Trial registration number ISRCTN60291412.

وصف الملف: electronic resource

Relation: https://bmjopen.bmj.com/content/14/1/e075821.full; https://doaj.org/toc/2044-6055

URL الوصول: https://doaj.org/article/11e7242718f54c27ab612f75f1df1340

المؤلفون: Ellen Clancy, Siva Ramadurai, Sarah R. Needham, Karen Baker, Tara A. Eastwood, Julia A. Weinstein, Daniel P. Mulvihill, Stanley W. Botchway

المصدر: Scientific Reports, Vol 13, Iss 1, Pp 1-15 (2023)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract Cytoplasmic viscosity is a crucial parameter in determining rates of diffusion-limited reactions. Changes in viscosity are associated with several diseases, whilst nuclear viscosity determines gene integrity, regulation and expression. Yet how drugs including DNA-damaging agents affect viscosity is unknown. We demonstrate the use of a platinum complex, Pt[L]Cl, that localizes efficiently mostly in the nucleus as a probe for nuclear viscosity. The phosphorescence lifetime of Pt[L]Cl is sensitive to viscosity and provides an excellent tool to investigate the impact of DNA damage. We show using Fluorescence Lifetime Imaging (FLIM) that the lifetime of both green and red fluorescent proteins (FP) are also sensitive to changes in cellular viscosity and refractive index. However, Pt[L]Cl proved to be a more sensitive viscosity probe, by virtue of microsecond phosphorescence lifetime versus nanosecond fluorescence lifetime of FP, hence greater sensitivity to bimolecular reactions. DNA damage was inflicted by either a two-photon excitation, one-photon excitation microbeam and X-rays. DNA damage of live cells causes significant increase in the lifetime of either Pt[L]Cl (HeLa cells, 12.5–14.1 µs) or intracellularly expressed mCherry (HEK293 cells, 1.54–1.67 ns), but a decrease in fluorescence lifetime of GFP from 2.65 to 2.29 ns (in V15B cells). These values represent a viscosity change from 8.59 to 20.56 cP as well as significant changes in the refractive index (RI), according to independent calibration. Interestingly DNA damage localized to a submicron region following a laser microbeam induction showed a whole cell viscosity change, with those in the nucleus being greater than the cytoplasm. We also found evidence of a by-stander effect, whereby adjacent un-irradiated cells also showed nuclear viscosity change. Finally, an increase in viscosity following DNA damage was also observed in bacterial cells with an over-expressed mNeonGreen FP, evidenced by the change in its lifetime from 2.8 to 2.4 ns.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2045-2322

URL الوصول: https://doaj.org/article/b8177410469a46f5968c335a1144f149

المؤلفون: Tara A. Eastwood, Karen Baker, Bree R. Streather, Nyasha Allen, Lin Wang, Stanley W. Botchway, Ian R. Brown, Jennifer R. Hiscock, Christopher Lennon, Daniel P. Mulvihill

المصدر: Cell Reports: Methods, Vol 3, Iss 2, Pp 100396- (2023)

مصطلحات موضوعية: CP: Biotechnology, CP: Molecular biology, Biotechnology, TP248.13-248.65, Biochemistry, QD415-436, Science

الوصف: Summary: We describe an innovative system that exports diverse recombinant proteins in membrane-bound vesicles from E. coli. These recombinant vesicles compartmentalize proteins within a micro-environment that enables production of otherwise challenging insoluble, toxic, or disulfide-bond containing proteins from bacteria. The release of vesicle-packaged proteins supports isolation from the culture and allows long-term storage of active protein. This technology results in high yields of vesicle-packaged, functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine. Motivation: The ability to reprogram a cell to direct the packaging of specific molecules into discrete membrane envelopes is one of the major challenges in the fields of synthetic biology and recombinant protein today. We thus set out to develop a system to allow the export of vesicle-packaged proteins from Escherichia coli. The resultant technology, involving a simple peptide tag, not only simplifies subsequent recombinant protein purification but the controlled packaging into membrane vesicles can be applied to the development of numerous technologies and commercializable products within the biotechnology and medical industries, including generation of recombinant bioreactors, environmental dispersion of biomolecules, and vehicles for drug delivery and vaccination, as well as providing a stable environment for isolation and storage of proteins.

وصف الملف: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2667237523000036; https://doaj.org/toc/2667-2375

URL الوصول: https://doaj.org/article/aaae0194c2e64ae396bd593598afc5b7

المؤلفون: Cherry Kilbride, Tom Butcher, Meriel Norris, Nana Anokye, Alyson Warland, Karen Baker, Dimitrios A Athanasiou, Guillem Singla-Buxarrais, Jennifer Ryan, Francesca Gowing, Carole Pound, Victoria Stewart, Basaam Aweid, Arul Samiyappan

المصدر: BMJ Open, Vol 12, Iss 6 (2022)

مصطلحات موضوعية: Medicine

وصف الملف: electronic resource

Relation: https://bmjopen.bmj.com/content/12/6/e058905.full; https://doaj.org/toc/2044-6055

URL الوصول: https://doaj.org/article/a17068a014b141f8aa0fe15a657eb55e

المؤلفون: Cherry Kilbride, Daniel J M Scott, Tom Butcher, Meriel Norris, Nana Anokye, Alyson Warland, Karen Baker, Dimitrios A Athanasiou, Guillem Singla-Buxarrais, Alexander Nowicky, Jennifer Ryan, Elizabeth Cassidy

المصدر: BMJ Open, Vol 12, Iss 2 (2022)

مصطلحات موضوعية: Medicine

وصف الملف: electronic resource

Relation: https://bmjopen.bmj.com/content/12/2/e052555.full; https://doaj.org/toc/2044-6055

URL الوصول: https://doaj.org/article/6727a3ec9a154ba392aebd1ceb91a78f

المؤلفون: Karen Baker, Irene A Gyamfi, Gregory I Mashanov, Justin E Molloy, Michael A Geeves, Daniel P Mulvihill

المصدر: eLife, Vol 8 (2019)

مصطلحات موضوعية: pombe, endocytosis, calmodulin, myosin, TORC2, Medicine, Science, Biology (General), QH301-705.5

الوصف: Cells respond to changes in their environment through signaling networks that modulate cytoskeleton and membrane organization to coordinate cell-cycle progression, polarized cell growth and multicellular development. Here, we define a novel regulatory mechanism by which the motor activity and function of the fission yeast type one myosin, Myo1, is modulated by TORC2-signalling-dependent phosphorylation. Phosphorylation of the conserved serine at position 742 (S742) within the neck region changes both the conformation of the neck region and the interactions between Myo1 and its associating calmodulin light chains. S742 phosphorylation thereby couples the calcium and TOR signaling networks that are involved in the modulation of myosin-1 dynamics to co-ordinate actin polymerization and membrane reorganization at sites of endocytosis and polarised cell growth in response to environmental and cell-cycle cues.

وصف الملف: electronic resource

Relation: https://elifesciences.org/articles/51150; https://doaj.org/toc/2050-084X

URL الوصول: https://doaj.org/article/bbfa004fdeba404386462db57bec03b4

المؤلفون: Karen Baker, Edmund Sabanegh Jr.

المصدر: Clinics, Vol 68, Pp 61-73 (2013)

مصطلحات موضوعية: Obstructive Azoospermia, Wolffian Duct Abnormalities, Iatrogenic Injury, Microsurgery, Vasovasostomy, Medicine (General), R5-920

الوصف: Obstructive azoospermia is a common cause of male infertility and can result from infection, congenital anomalies, or iatrogenic injury. Microsurgical vasal reconstruction is a suitable treatment for many cases of obstructive azoospermia, although some couples will require sperm retrieval paired with in-vitro fertilization. The various causes of obstructive azoospermia and recommended treatments will be examined. Microsurgical vasovasostomy and vasoepididymostomy will be discussed in detail. The postoperative patency and pregnancy rates for surgical reconstruction of obstructive azoospermia and the impact of etiology, obstructive interval, sperm granuloma, age, and previous reconstruction on patency and pregnancy will be reviewed.

وصف الملف: electronic resource

Relation: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322013001300007; https://doaj.org/toc/1807-5932; https://doaj.org/toc/1980-5322

URL الوصول: https://doaj.org/article/62ed1e0ee11a4b7d81d97ea00bd98531

المؤلفون: Bree R. Streather, author¹, Karen Baker, author¹, Tara A. Eastwood, author¹, Daniel P. Mulvihill, author¹

المصدر: Journal of Visualized Experiments. (196)

الإتاحة: http://www.jove.com/video/65442

المؤلفون: Karen Baker-Fletcher

المصدر: Modern Believing. 63:389-398

مصطلحات موضوعية: General Medicine

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::6376ea4cf5c1230779ff4490c2e12b1a
https://doi.org/10.3828/mb.2022.30

المؤلفون: Karen Baker, John Stephenson

المصدر: British Journal of Midwifery. 30:250-256

مصطلحات موضوعية: Maternity and Midwifery

الوصف: Background National and international guidelines advise active management of the third stage of labour. Studies conducted in obstetric-led units reveal evidence for reductions in primary postpartum haemorrhage with active management compared to expectant management. The association in midwife-led units for low-risk women is relatively untested in the UK. Aims To examine the relationship between third stage of labour management approaches, and incidence of postpartum haemorrhage and severe postpartum haemorrhage in women birthing in midwife-led units. Methods Anonymised data were collected in 2015–2016 from women birthing in England. Adjusted logistic regression was used to assess the effect of management approach on the outcomes of postpartum haemorrhage and severe postpartum haemorrhage, controlling for variables additionally identified as risk factors for postpartum haemorrhage. Results Of the 765 women intending to receive active management and the 508 intending to receive expectant management, 9.54% and 14.0% experienced postpartum haemorrhage respectively (P=0.015). Severe postpartum haemorrhage was experienced by 14 (1.83%) women intending to receive active management and 16 (3.66%) intending to receive expectant management (P=0.134). Conclusions Postpartum haemorrhage, but not severe postpartum haemorrhage, was statistically significantly higher in women birthing in midwife-led units intending to receive expectant management.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::40ce6fd949f5fe0a8274874dab171dd7
https://doi.org/10.12968/bjom.2022.30.5.250