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1دورية أكاديمية
المؤلفون: Kamboj, M, Bohlke, K, Baptiste, DM, Dunleavy, K, Fueger, A, Jones, L, Kelkar, AH, Law, LY, LeFebvre, KB, Ljungman, P, Miller, ED, Meyer, LA, Moore, HN, Soares, HP, Taplitz, RA, Woldetsadik, ES, Kohn, EC
المصدر: Journal of clinical oncology : official journal of the American Society of Clinical Oncology. 42(14):1699-1721
مصطلحات موضوعية: Medicin och hälsovetenskap
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2دورية أكاديمية
المؤلفون: Kassis, J, Klominek, J, Kohn, EC
المصدر: Diagnostic cytopathology. 33(5):316-319
مصطلحات موضوعية: Medicin och hälsovetenskap
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3دورية أكاديمية
المؤلفون: Kohn, EC, Travers, LA, Kassis, J, Broome, U, Klominek, J
المصدر: Diagnostic cytopathology. 33(5):300-308
مصطلحات موضوعية: Medicin och hälsovetenskap
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4
المؤلفون: MINEO, Marco, TAVERNA, Simona, FLUGY PAPE', Anna Maria, DE LEO, Giacomo, ALESSANDRO, Riccardo, Garfield, SH, Kohn, EC
المساهمون: Mineo, M, Garfield, SH, Taverna, S, Flugy Pape', AM, De Leo, G, Alessandro, R, Kohn, EC
مصطلحات موضوعية: Exosomes, Nanotubes, Chronic myeloid leukemia, Endothelial cells, Tyrosine kinase inhibitors
الوصف: Exosomes, microvesicles of endocytic origin released by normal and tumor cells, play an important role in cell-to-cell ommunication. Angiogenesis has been shown to regulate progression of chronic myeloid leukemia (CML). The mechanism through which this happens has not been elucidated. We isolated and characterized exosomes from K562 CML cells and evaluated their effects on human umbilical endothelial cells (HUVECs). Fluorescent-labeled exosomes were nternalized by HUVECs during tubular differentiation on Matrigel. Exosome localization was perinuclear early in differentiation, moving peripherally in cells undergoing elongation and connection. Exosomes move within and between nanotubular structures connecting the remodeling endothelial cells. They stimulated angiotube formation over a serum/growth factor-limited medium control,doubling total cumulative tube length (P = 0.003). Treatment of K562 cells with two clinically active tyrosine kinase inhibitors, imatinib and dasatinib, reduced their total exosome release (P\0.009); equivalent concentrations of drug-treated exosomes induced a similar extent of tubular differentiation. However, dasatinib treatment of HUVECs markedly inhibited HUVEC response to drug control CML exosomes (P\0.002). In an in vivo mouse matrigel plug model angiogenesis was induced by K562 exosomes and abrogated by oral dasatinib treatment (P\0.01). K562 exosomes induced dasatinib-sensitive Src phosphorylation and activation of downstream Src pathway proteins in HUVECs. Imatinib was minimally active against exosome stimulation of HUVEC cell differentiation and signaling. Thus, CML cell-derived exosomes induce angiogenic activity in HUVEC cells. The inhibitory effect of dasatinib on exosome production and vascular differentiation and signaling reveals a key role for Src in both the leukemia and its microenvironment.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::963e88d6642d3ac462fdd84b86db924a
http://hdl.handle.net/10447/62152 -
5
المؤلفون: FONTANA, Simona, BARRANCA, Marilisa, CORRADO, Chiara, DE LEO, Giacomo, ALESSANDRO, Riccardo, Zannellia Cleon, I, Becchi, M, Kohn, EC
المساهمون: Fontana, S, Barranca, M, Corrado, C, Zannellia-Cleon, I, Becchi, M, Kohn, EC, De Leo, G, Alessandro, R
URL الوصول: https://explore.openaire.eu/search/publication?articleId=od______3658::167d95e61be56a512f26f9db03e914ce
http://hdl.handle.net/10447/56319 -
6
المؤلفون: FONTANA, Simona, CORRADO, Chiara, DE LEO, Giacomo, ALESSANDRO, Riccardo, Barranca, M, Zanella Cleon, F, Becchi, M, Kohn, EC
المساهمون: Fontana, S, Barranca, M, Corrado, C, Zanella-Cleon, F, Becchi, M, Kohn, EC, De Leo, G, Alessandro, R
مصطلحات موضوعية: Settore BIO/13 - Biologia Applicata, leucemia mieloide cronica, resistenza all'imatinib, CAI
URL الوصول: https://explore.openaire.eu/search/publication?articleId=od______3658::f08dca0b9b3fdb2b520ae87017a95ddb
http://hdl.handle.net/10447/64295 -
7
المؤلفون: ALESSANDRO, Riccardo, FONTANA, Simona, BARRANCA, Marilisa, CORRADO, Chiara, GIORDANO, Margherita, DE LEO, Giacomo, Zanella Cleon, I, Becchi, M, Kohn, EC
المساهمون: Alessandro, R, Fontana, S, Barranca, M, Corrado, C, Giordano, M, Zanella Cleon, I, Becchi, M, Kohn, EC, De Leo, G
مصطلحات موضوعية: chronic myelogenous leukemia, Proteome profiling
URL الوصول: https://explore.openaire.eu/search/publication?articleId=od______3658::1646a7027c5434b45080d3d27dbe8aa6
http://hdl.handle.net/10447/37966 -
8
المؤلفون: FONTANA, Simona, ALESSANDRO, Riccardo, BARRANCA, Marilisa, GIORDANO, Margherita, CORRADO, Chiara, DE LEO, Giacomo, Zanella Cleon, I, Becchi, M, Kohn, EC
المساهمون: Fontana, S, Alessandro, R, Barranca, M, Giordano, M, Corrado, C, Zanella-Cleon, I, Becchi, M, Kohn, EC, De Leo, G
مصطلحات موضوعية: Chronic Myelogenous Leukemia, proteomic analysis
URL الوصول: https://explore.openaire.eu/search/publication?articleId=od______3658::37965472321e2d47827a32b0f65d52c8
http://hdl.handle.net/10447/37807 -
9
المؤلفون: CORRADO, Chiara, FONTANA, Simona, COLOMBA, Paolo, DE LEO, Giacomo, ALESSANDRO, Riccardo, Giordano, M, RAIMONDO, Stefania, Arlinghaus, RB, Kohn, EC
المساهمون: Corrado,C, Fontana, S, Giordano, M, Colomba, P, Raimondo, S, Arlinghaus, RB, Kohn, EC, De Leo, G, Alessandro, R
مصطلحات موضوعية: carboxyamdotriazole, Imatinib resistance, Chronic Myelogenous Leukemia
URL الوصول: https://explore.openaire.eu/search/publication?articleId=od______3658::f9047fd28a156e0aaaf278b829c5bc3d
http://hdl.handle.net/10447/44408 -
10
المؤلفون: FONTANA, Simona, BARRANCA, Marilisa, GIORDANO, Margherita, CORRADO, Chiara, DE LEO, Giacomo, ALESSANDRO, Riccardo, ZANELLA CLEON I, BECCHI M, KOHN EC
المساهمون: FONTANA S, BARRANCA M, GIORDANO M, FLUGY A M, CORRADO C, BECCHI M, KOHN EC, DE LEO G, ALESSANDRO R, ZANELLA-CLEON I, DE LEO
URL الوصول: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::114c1c5f440cfba1f419c89ae6cfe36d
http://hdl.handle.net/10447/32638