المؤلفون: Laustsen, A, Bak, RO, Krapp, C, Kjær, L, Egedahl, JH, Petersen, CC, Pillai, S, Tang, HQ, Uldbjerg, N, Porteus, M, Roan, NR, Nyegaard, M, Denton, PW, Jakobsen, MR

المصدر: Nature communications. 9(1)

مصطلحات موضوعية: Dendritic Cells, Cells, Cultured, Humans, Nucleotidyltransferases, Interferon Type I, Antigens, CD34, Interleukin-6, Enzyme-Linked Immunosorbent Assay, Polymerase Chain Reaction, Cell Differentiation, Toll-Like Receptor 7, Toll-Like Receptor 9, Receptor, Interferon alpha-beta, CRISPR-Cas Systems, DEAD Box Protein 58, Gene Editing, Cells, Cultured, Antigens, CD34, Receptor, Interferon alpha-beta, Stem Cell Research - Nonembryonic - Human, Stem Cell Research, Rare Diseases, Regenerative Medicine, Genetics, 1.1 Normal biological development and functioning, Inflammatory and Immune System, MD Multidisciplinary

الوصف: Plasmacytoid dendritic cells (pDC) are essential for immune competence. Here we show that pDC precursor differentiated from human CD34+ hematopoietic stem and progenitor cells (HSPC) has low surface expression of pDC markers, and has limited induction of type I interferon (IFN) and IL-6 upon TLR7 and TLR9 agonists treatment; by contrast, cGAS or RIG-I agonists-mediated activation is not altered. Importantly, after priming with type I and II IFN, these precursor pDCs attain a phenotype and functional activity similar to that of peripheral blood-derived pDCs. Data from CRISPR/Cas9-mediated genome editing of HSPCs further show that HSPC-pDCs with genetic modifications can be obtained, and that expression of the IFN-α receptor is essential for the optimal function, but dispensable for the differentiation, of HSPC-pDC percursor. Our results thus demonstrate the biological effects of IFNs for regulating pDC function, and provide the means of generating of gene-modified human pDCs.

وصف الملف: application/pdf

URL الوصول: https://escholarship.org/uc/item/3979k69n

المؤلفون: Heimann, P., Krapp, C.-A., Westfechtel, B.

المصدر: Proceedings 8th Conference on Software Engineering Environments Software engineering environments Software Engineering Environments, Eighth Conference on. :101-109 1997

Relation: Proceedings 8th Conference on Software Engineering Environments

المؤلفون: Heimann, P., Joeris, G., Krapp, C.-A., Westfechtel, B.

المصدر: Proceedings of IEEE 18th International Conference on Software Engineering Software engineering Software Engineering, 1996., Proceedings of the 18th International Conference on. :331-341 1996

Relation: Proceedings of IEEE 18th International Conference on Software Engineering

المؤلفون: Krapp, C.-A., Westfechtel, B.

المصدر: Proceedings 12th IEEE International Conference Automated Software Engineering Automated software engineering Automated Software Engineering, 1997. Proceedings., 12th IEEE International Conference. :301-302 1997

Relation: Proceedings 12th IEEE International Conference Automated Software Engineering

المؤلفون: Heller, M., Jäger, D., Krapp, C. -A., Nagl, M., Schleicher, A., Westfechtel, B., Wörzberger, R.

المساهمون: Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Pandu Rangan, C., editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Doug, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, Nagl, Manfred, editor, Marquardt, Wolfgang, editor

المصدر: Collaborative and Distributed Chemical Engineering. From Understanding to Substantial Design Process Support : Results of the IMPROVE Project. 4970:300-366

المؤلفون: Starodub, A.N., Kim, S-B., Davar, D., Powderly, J., Hill, A.G., Yeku, O., Gabrail, N., Krapp, C., Pihl, S., Chen, M., Slavsky, S., Torrejon Castro, D., Singel, S.M., Kwatra, V.

المصدر: In Annals of Oncology October 2023 34 Supplement 2:S628-S628

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المؤلفون: Biolatti, M, Dell'Oste, V, Pautasso, S, Gugliesi, F, von Einem, J, Krapp, C, Jakobsen, Mr, Borgogna, C, Gariglio, M, De Andrea, M, Landolfo, S

URL الوصول: https://explore.openaire.eu/search/publication?articleId=od_______970::12f96e9c48b8f175dc97e20b4bd2e357
http://hdl.handle.net/2318/1660036

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