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1دورية أكاديمية
المؤلفون: Bingyu Gu, Haowen Wang, Jiayi Lv, Yanfen Zheng, Xiao-Hua Zhang, Yuanchao Zhan
المصدر: Frontiers in Marine Science, Vol 11 (2024)
مصطلحات موضوعية: bacteriophage induction, deep-sea, genomic analysis, Labrenzia aggregata, Labrenmarinevirus, Science, General. Including nature conservation, geographical distribution, QH1-199.5
الوصف: In marine environments, viruses play a pivotal role, yet deep-sea bacteriophages remains largely uncharacterized. The bacterium Labrenzia aggregata RF14, isolated from the Mariana Trench at a depth of 4,000 meters, harbors prophage regions based on a previous study. In this study, we induced a temperate bacteriophage from it using mitomycin C. The bacteriophage exhibited an icosahedral structure with a non-extendable tail and was named vB_LagS-V1. The genome size of it is 39,329 bps with a 59.46% G+C content, encoding 60 putative open reading frames. Genomic and phylogenetic analyses demonstrated that vB_LagS-V1 along with many bacteriophages infecting Hyphomicrobiales, constituted a newly unclassified family, which we designated as Hyphoviridae. Within this novel family, vB_LagS-V1 is distinct with isolated phages and clustered with two uncultured prophages within Labrenzia, forming an unclassified new genus, given a name of Labrenmarinevirus. The codon usage correlation and absence of tRNAs found in vB_LagS-V1, also prevail in some deep-sea bacteriophages, highlighting their adaptations to the deep-sea prokaryotic hosts. Moreover, vB_LagS-V1 encoded two auxiliary metabolic genes, cysteine dioxygenase and phosphoadenosine phosphosulfate reductase, which might help the phage and its host adapt to high hydrostatic pressure in the deep-sea environments. Our study will significantly contribute to the understanding of deep-sea bacteriophages and their interactions with hosts in extreme environments.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Haohui Zhong, Hao Sun, Ronghua Liu, Yuanchao Zhan, Xinyu Huang, Feng Ju, Xiao-Hua Zhang
المصدر: Frontiers in Microbiology, Vol 12 (2021)
مصطلحات موضوعية: Labrenzia aggregata, the Mariana Trench, sulfur cycle, nitrogen cycle, metabolic capacity, hadal zone, Microbiology, QR1-502
الوصف: Hadal zones are marine environments deeper than 6,000 m, most of which comprise oceanic trenches. Microbes thriving at such depth experience high hydrostatic pressure and low temperature. The genomic potentials of these microbes to such extreme environments are largely unknown. Here, we compare five complete genomes of bacterial strains belonging to Labrenzia aggregata (Alphaproteobacteria), including four from the Mariana Trench at depths up to 9,600 m and one reference from surface seawater of the East China Sea, to uncover the genomic potentials of this species. Genomic investigation suggests all the five strains of L. aggregata as participants in nitrogen and sulfur cycles, including denitrification, dissimilatory nitrate reduction to ammonium (DNRA), thiosulfate oxidation, and dimethylsulfoniopropionate (DMSP) biosynthesis and degradation. Further comparisons show that, among the five strains, 85% gene functions are similar with 96.7% of them encoded on the chromosomes, whereas the numbers of functional specific genes related to osmoregulation, antibiotic resistance, viral infection, and secondary metabolite biosynthesis are majorly contributed by the differential plasmids. A following analysis suggests the plasmidic gene numbers increase along with isolation depth and most plasmids are dissimilar among the five strains. These findings provide a better understanding of genomic potentials in the same species throughout a deep-sea water column and address the importance of externally originated plasmidic genes putatively shaped by deep-sea environment.
وصف الملف: electronic resource
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3دورية أكاديمية
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4دورية أكاديميةTranscriptome analysis revealed transporter proteins role in the growth of Labrenzia sp. PO1 and SY1
المؤلفون: Madiha Zaynab, Huirong Chen, Yufei Chen, Liao Ouyang, Xuewei Yang, Wang Xu, Qinghuai Zeng, Khalid Ali Khan, Mohamed M. Hassan, Sabry Hassan, Shuangfei Li
المصدر: Journal of King Saud University: Science, Vol 33, Iss 4, Pp 101433- (2021)
مصطلحات موضوعية: Akashiwo sanguinea, Comparative Transcriptomic, Differentially Expressed Genes (DEGs), Labrenzia sp. Transporters, Science (General), Q1-390
الوصف: Akashiwo sanguinea is closely linked to the healthy growth of Labrenzia sp. PO1. Herein, PO1 and SY1 strains of Labrenzia sp. were isolated from A. sanguinea to investigate their growth. A total of 6067 genes in the Labrenzia sp SY1 and 6067 genes in Labrenzia sp PO1 were identified by RNA sequencing. Totally, 2402 genes out of 6067 were found as differentially expressed genes (DEGs). PO1 strain, 595 with decreased transcript abundance, and 1807 increase transcript abundance. Total 2320 DEGs, 766 with decreased transcript abundance, and 1554 increase transcript abundance were found in the SY1 strain. Transcriptome analysis divulged that DEGs were enriched with regulating metabolic processes, biological processes, and binding activities and may play critical roles in Labrenzia sp. PO1 growth. The ABC transporter pathway was also enriched in PO1 with 23 genes encoding ABC transporters with increased transcript abundance only in this strain. ABC transporter transcript levels and Labrenzia sp. PO1 growth was highest at 32 h, indicating that transporter-encoding genes may be involved in PO1 growth through nutrient transport. Moreover, their low expression in SY1 may be related to its slower growth. This study of Labrenzia sp. PO1 and SY1 transcriptomes may be used as a foundation for future research on the biofilm formation phenomenon of Labrenzia sp. PO1, as the formation was observed only in PO1.
وصف الملف: electronic resource
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5دورية أكاديمية
المؤلفون: Romanenko, Lyudmila A.Aff1, Kurilenko, Valeriya V., Guzev, Konstantin V., Svetashev, Vassilii I.
المصدر: Archives of Microbiology. 201(5):705-712
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6دورية أكاديمية
المؤلفون: Caitlin A. Lawson, Justin R. Seymour, Malcolm Possell, David J. Suggett, Jean-Baptiste Raina
المصدر: Frontiers in Marine Science, Vol 7 (2020)
مصطلحات موضوعية: volatilome, Labrenzia sp., Marinobacter adhaerens HP15, GC–MS, core microbiome, Symbiodiniaceae, Science, General. Including nature conservation, geographical distribution, QH1-199.5
الوصف: Biogenic volatile organic compounds (BVOCs) are a large group of molecules involved in trophic interactions, stress response and atmospheric chemistry. Although they have been extensively studied in terrestrial ecosystems, their identity and prevalence in the marine environment remains largely unexplored. Here we characterized the volatilome of two abundant marine bacteria that were previously identified as members of the core microbiome of Symbiodiniaceae (phylum: Dinoflagellata), the photosynthetic endosymbionts of reef building corals. To determine the influence of Symbiodiniaceae exudate on their associated bacteria, we incubated isolates of Marinobacter adhaerens HP15 and Labrenzia sp. 21p with Symbiodiniaceae culture filtrate or culture medium (control) and investigated their volatilomes using GC–MS. The volatilome of Labrenzia sp. incubated in Symbiodiniaceae filtrate was significantly different and more diverse relative to the control. In contrast, the overall composition of the M. adhaerens volatilomes were consistent between treatment and control. Among the 35 compounds detected in both bacterial species, the dominant chemical functional groups were halogenated hydrocarbons, aromatic hydrocarbons and organosulfurs, some of which are known to play roles in inter-organism signaling, to act as antioxidants and as antimicrobials. This study provides new insights into the potential sources and diversity of marine BVOCs, uncovering a wide range of molecules that may play important physiological and ecological roles for these organisms, while also revealing the role of Symbiodiniaceae-associated bacteria in the emission of important atmospheric gases.
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: Carolina Díaz-Cárdenas, Laura Yinneth Rojas, Susana Fiorentino, Monica P. Cala, Jorge I Díaz, Freddy A. Ramos, Jean Armengaud, Silvia Restrepo, Sandra Baena
المصدر: Molecules, Vol 25, Iss 11, p 2546 (2020)
مصطلحات موضوعية: Labrenzia aggregata USBA 371, cytotoxic activities, bioactive natural products, saline natural products, secondary metabolism, genome annotation, Organic chemistry, QD241-441
الوصف: Previous studies revealed the potential of Labrenzia aggregata USBA 371 to produce cytotoxic metabolites. This study explores its metabolic diversity and compounds involved in its cytotoxic activity. Extracts from the extracellular fraction of strain USBA 371 showed high levels of cytotoxic activity associated with the production of diketopiperazines (DKPs). We purified two compounds and a mixture of two other compounds from this fraction. Their structures were characterized by 1D and 2D nuclear magnetic resonance (NMR). The purified compounds were evaluated for additional cytotoxic activities. Compound 1 (cyclo (l-Pro-l-Tyr)) showed cytotoxicity to the following cancer cell lines: breast cancer 4T1 (IC50 57.09 ± 2.11 µM), 4T1H17 (IC50 40.38 ± 1.94), MCF-7 (IC50 87.74 ± 2.32 µM), murine melanoma B16 (IC50 80.87 ± 3.67), human uterus sarcoma MES-SA/Dx5 P-pg (−) (IC50 291.32 ± 5.64) and MES-SA/Dx5 P-pg (+) (IC50 225.28 ± 1.23), and murine colon MCA 38 (IC50 29.85 ± 1.55). In order to elucidate the biosynthetic route of the production of DKPs and other secondary metabolites, we sequenced the genome of L. aggregata USBA 371. We found no evidence for biosynthetic pathways associated with cyclodipeptide synthases (CDPSs) or non-ribosomal peptides (NRPS), but based on proteogenomic analysis we suggest that they are produced by proteolytic enzymes. This is the first report in which the cytotoxic effect of cyclo (l-Pro-l-Tyr) produced by an organism of the genus Labrenzia has been evaluated against several cancer cell lines.
وصف الملف: electronic resource
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8دورية أكاديمية
المؤلفون: Zhangran Chen, Wei Zheng, Luxi Yang, Lisa A. Boughner, Yun Tian, Tianling Zheng, Hong Xu
المصدر: Frontiers in Microbiology, Vol 8 (2017)
مصطلحات موضوعية: Phaeodactylum tricornutum, Labrenzia sp. KD531, chemotaxis, algal biomass, algal plaque, Microbiology, QR1-502
الوصف: Phaeodactylum tricornutum is a dominant bloom forming species and potential biofuel feedstock. To control P. tricornutum bloom or to release lipids from P. tricornutum, we previously screened and identified the lytic bacterium Labrenzia sp. KD531 toward P. tricornutum. In the present study, we evaluated the lytic activity of Labrenzia sp. KD531 on microalgae and investigated its lytic mechanism. The results indicated that the lytic activity of KD531 was temperature- and pH-dependent, but light-independent. In addition to P. tricornutum, KD531 also showed lytic activity against other algal species, especially green algae. A quantitative analysis of algal cellular protein, carbohydrate and lipid content together with measurements of dry weight after exposure to bacteria-infected algal lysate indicated that the bacterium KD531 influenced the algal biomass by disrupting the algal cells. Both chemotactic analysis and microscopic observations of subsamples from different regions of formed plaques showed that KD531 could move toward and then directly contact algal cells. Direct contact between P. tricornutum and KD531 cells was essential for the lytic process.
وصف الملف: electronic resource
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9دورية أكاديمية
المصدر: Sensors, Vol 14, Iss 7, Pp 11760-11769 (2014)
مصطلحات موضوعية: Labrenzia sp., quorum quenching, quorum sensing, marine, seawater, rapid resolution liquid chromatography (RRLC), N-acylhomoserine lactone, N-(3-oxohexanoyl)-l-homoserine lactone, Chemical technology, TP1-1185
الوصف: We report the degradation of quorum sensing N-acylhomoserine lactone molecules by a bacterium isolated from a Malaysian marine water sample. MALDI-TOF and phylogenetic analysis indicated this isolate BM1 clustered closely to Labrenzia sp. The quorum quenching activity of this isolate was confirmed by using a series of bioassays and rapid resolution liquid chromatography analysis. Labrenzia sp. degraded a wide range of N-acylhomoserine lactones namely N-(3-hexanoyl)-l-homoserine lactone (C6-HSL), N-(3-oxohexanoyl)-l-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-l-homoserine lactone (3-hydroxy-C6-HSL). Re-lactonisation bioassays confirmed Labrenzia sp. BM1 degraded these signalling molecules efficiently via lactonase activity. To the best of our knowledge, this is the first documentation of a Labrenzia sp. capable of degrading N-acylhomoserine lactones and confirmation of its lactonase-based mechanism of action.
وصف الملف: electronic resource
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10دورية أكاديمية
المؤلفون: Jamshid Amiri Moghaddam, Antonio Dávila-Céspedes, Mohammad Alanjary, Jochen Blom, Gabriele M. König, Till F. Schäberle
المصدر: Data, Vol 4, Iss 1, p 33 (2019)
مصطلحات موضوعية: Labrenzia, draft genome, comparative genomics, antimicrobial, oyster disease, Roseovarius crassostreae, Bibliography. Library science. Information resources
الوصف: The marine bacterium Labrenzia sp. strain 011 was isolated from the coastal sediment of Kronsgaard, Germany. The Labrenzia species are suggested to be protective agents of mollusks. Labrenzia sp. strain 011 produces specialized metabolites, which showed activity against a range of microorganisms, thereunder strong inhibitory effects against Pseudoroseovarius crassostreae DSM 16,950 (genus Roseovarius), the causative agent of oyster disease. The genome of Labrenzia sp. strain 011 was sequenced and assembled into 65 contigs, has a size of 5.1 Mbp, and a G+C content of 61.6%. A comparative genome analysis defined Labrenzia sp. strain 011 as a distinct new species within the genus Labrenzia, whereby 44% of the genome was contributed to the Labrenzia core genome. The genomic data provided here is expected to contribute to a deeper understanding of the mollusk-protective role of Labrenzia spp.
وصف الملف: electronic resource
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