المؤلفون: Egger, Matthias^{Aff1, Aff2, Aff3}, Salmanton-García, Jon^{Aff4, Aff5, Aff37}, Barac, Aleksandra, Gangneux, Jean-Pierre, Guegan, Hélène, Arsic-Arsenijevic, Valentina^{Aff8, Aff9}, Matos, Tadeja, Tomazin, Rok, Klimko, Nikolai, Bassetti, Matteo^{Aff12, Aff13}, Hammarström, Helena, Meijer, Eelco F. J.^{Aff15, Aff16, Aff17}, Meis, Jacques F.^{Aff15, Aff16, Aff17}, Prattes, Juergen^{Aff1, Aff2, Aff3}, Krause, Robert^{Aff1, Aff2, Aff3}, Resat Sipahi, Oguz, Scharmann, Ulrike, White, P. Lewis, Desoubeaux, Guillaume, García-Rodríguez, Julio, Garcia-Vidal, Carolina, Martín-Pérez, Sonia, Ruiz, Maite^{Aff25, Aff26}, Tumbarello, Mario, Talento, Alida Fe, Rogers, Benedict, Lagrou, Katrien^{Aff30, Aff31}, van Praet, Jens, Arikan-Akdagli, Sevtap, Arendrup, Maiken C.^{Aff34, Aff35, Aff36}, Koehler, Philipp^{Aff4, Aff5, Aff37}, Cornely, Oliver A.^{Aff4, Aff5, Aff37, Aff38}, Hoenigl, Martin^{Aff1, Aff2, Aff3, IDs11046023007764_cor33}

المصدر: Mycopathologia. 188(6):983-994

المؤلفون: Aerts, Robina^{Aff1, Aff2}, Cuypers, Lize^{Aff2, Aff3}, Mercier, Toine^{Aff2, Aff4}, Maertens, Johan^{Aff2, Aff5}, Lagrou, Katrien^{Aff2, Aff3, IDs11046023007399_cor5}

المصدر: Mycopathologia. 188(5):655-665

المؤلفون: Giacobbe, Daniele Roberto^{Aff1, Aff2, IDs13054023046081_cor1}, Dettori, Silvia^{Aff1, Aff2}, Di Pilato, Vincenzo, Asperges, Erika, Ball, Lorenzo^{Aff3, Aff5}, Berti, Enora, Blennow, Ola^{Aff7, Aff8}, Bruzzone, Bianca, Calvet, Laure, Capra Marzani, Federico, Casabella, Antonio, Choudaly, Sofia, Dartevel, Anais, De Pascale, Gennaro^{Aff15, Aff16}, Di Meco, Gabriele, Fallon, Melissa, Galerneau, Louis-Marie, Gallego, Miguel^{Aff18, Aff19}, Giacomini, Mauro, González Sáez, Adolfo^{Aff21, Aff22}, Hänsel, Luise^{Aff23, Aff24}, Icardi, Giancarlo^{Aff1, Aff9}, Koehler, Philipp^{Aff23, Aff24}, Lagrou, Katrien^{Aff25, Aff26}, Lahmer, Tobias, Lewis White, P.^{Aff17, Aff28}, Magnasco, Laura, Marchese, Anna^{Aff3, Aff29}, Marelli, Cristina, Marín-Arriaza, Mercedes^{Aff21, Aff22, Aff30}, Martin-Loeches, Ignacio^{Aff31, Aff32}, Mekontso-Dessap, Armand^{Aff6, Aff33, Aff34}, Mikulska, Malgorzata^{Aff1, Aff2}, Mularoni, Alessandra, Nordlander, Anna^{Aff7, Aff8}, Poissy, Julien^{Aff13, Aff36}, Russelli, Giovanna, Signori, Alessio, Tascini, Carlo^{Aff38, Aff39}, Vaconsin, Louis-Maxime, Vargas, Joel, Vena, Antonio^{Aff1, Aff2}, Wauters, Joost^{Aff25, Aff41}, Pelosi, Paolo^{Aff3, Aff5}, Timsit, Jean-Francois^{Aff40, Aff42}, Bassetti, Matteo^{Aff1, Aff2}

المصدر: Critical Care. 27(1)

المؤلفون: Janssen, Reile, Cuypers, Lize, Laenen, Lies, Keyaerts, Els, Beuselinck, Kurt, Janssenswillen, Sunita, Slechten, Bram, Bode, Jannes, Wollants, Elke, Van Laethem, Kristel, Rector, Annabel, Bloemen, Mandy, Sijmons, Anke, de Schaetzen, Nathalie, Capron, Arnaud, Van Baelen, Kurt, Pascal, Thierry, Vermeiren, Céline, Bureau, Fabrice, Vandesompele, Jo, De Smet, Pieter, Uten, Wouter, Malonne, Hugues, Kerkhofs, Pierre, De Cock, Jo, Matheeussen, Veerle, Verhasselt, Bruno, Gillet, Laurent, Detry, Gautier, Bearzatto, Bertrand, Degosserie, Jonathan, Henin, Coralie, Pairoux, Gregor, COVID-19 Genomics Belgium Consortium, Maes, Piet, Van Ranst, Marc, Lagrou, Katrien, Dequeker, Elisabeth, André, Emmanuel

المصدر: Virology Journal, 21 (1), 40 (2024-02-10)

مصطلحات موضوعية: Belgium, COVID-19, High-throughput testing, National reference centre, Quality assurance, SARS-CoV-2, Humans, Belgium/epidemiology, COVID-19 Testing, Pandemics, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, SARS-CoV-2/genetics, COVID-19/diagnosis, COVID-19/epidemiology, Virology, Infectious Diseases, Human health sciences, Public health, health care sciences & services, Sciences de la santé humaine, Santé publique, services médicaux & soins de santé

الوصف: Since the onset of the coronavirus disease (COVID-19) pandemic in Belgium, UZ/KU Leuven has played a crucial role as the National Reference Centre (NRC) for respiratory pathogens, to be the first Belgian laboratory to develop and implement laboratory developed diagnostic assays for SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) and later to assess the quality of commercial kits. To meet the growing demand for decentralised testing, both clinical laboratories and government-supported high-throughput platforms were gradually deployed across Belgium. Consequently, the role of the NRC transitioned from a specialised testing laboratory to strengthening capacity and coordinating quality assurance. Here, we outline the measures taken by the NRC, the national public health institute Sciensano and the executing clinical laboratories to ensure effective quality management of molecular testing throughout the initial two years of the pandemic (March 2020 to March 2022).

Relation: https://link.springer.com/content/pdf/10.1186/s12985-024-02308-y.pdf; urn:issn:1743-422X

URL الوصول: https://orbi.uliege.be/handle/2268/315659

المؤلفون: Aerts, Robina, Mehra, Varun, Groll, Andreas H, Martino, Rodrigo, Lagrou, Katrien, Robin, Christine, Perruccio, Katia, Blijlevens, Nicole, Nucci, Marcio, Slavin, Monica, Bretagne, Stéphane, Cordonnier, Catherine ^*

المصدر: In The Lancet Infectious Diseases May 2024 24(5):e291-e306

المؤلفون: Giacobbe, Daniele Roberto, Prattes, Juergen, Wauters, Joost, Dettori, Silvia, Signori, Alessio, Salmanton-García, Jon, Maertens, Johan, Bourgeois, Marc, Reynders, Marijke, Rutsaert, Lynn, Van Regenmortel, Niels, Lormans, Piet, Feys, Simon, Klimko, Nikolay, Shadrivova, Olga, Cornely, Oliver A, Rautemaa-Richardson, Riina, Koehler, Philipp, Lagrou, Katrien, Bassetti, Matteo, Hoenigl, Martin

المصدر: Journal of Clinical Microbiology. 60(4)

مصطلحات موضوعية: Infectious Diseases, Rare Diseases, Lung, Good Health and Well Being, Aspergillus, Bronchoalveolar Lavage Fluid, COVID-19, Critical Illness, Galactose, Humans, Intensive Care Units, Invasive Pulmonary Aspergillosis, Mannans, Mycology, Prognosis, Pulmonary Aspergillosis, Sensitivity and Specificity, CAPA, GM, biomarker, galactomannan, BALF, ECMM-CAPA Study Group, Biological Sciences, Agricultural and Veterinary Sciences, Medical and Health Sciences, Microbiology

الوصف: Critically ill patients with coronavirus disease 2019 (COVID-19) may develop COVID-19-associated pulmonary aspergillosis (CAPA), which impacts their chances of survival. Whether positive bronchoalveolar lavage fluid (BALF) mycological tests can be used as a survival proxy remains unknown. We conducted a post hoc analysis of a previous multicenter, multinational observational study with the aim of assessing the differential prognostic impact of BALF mycological tests, namely, positive (optical density index of ≥1.0) BALF galactomannan (GM) and positive BALF Aspergillus culture alone or in combination for critically ill patients with COVID-19. Of the 592 critically ill patients with COVID-19 enrolled in the main study, 218 were included in this post hoc analysis, as they had both test results available. CAPA was diagnosed in 56/218 patients (26%). Most cases were probable CAPA (51/56 [91%]) and fewer were proven CAPA (5/56 [9%]). In the final multivariable model adjusted for between-center heterogeneity, an independent association with 90-day mortality was observed for the combination of positive BALF GM and positive BALF Aspergillus culture in comparison with both tests negative (hazard ratio, 2.53; 95% CI confidence interval [CI], 1.28 to 5.02; P = 0.008). The other independent predictors of 90-day mortality were increasing age and active malignant disease. In conclusion, the combination of positive BALF GM and positive BALF Aspergillus culture was associated with increased 90-day mortality in critically ill patients with COVID-19. Additional study is needed to explore the possible prognostic value of other BALF markers.

وصف الملف: application/pdf

URL الوصول: https://escholarship.org/uc/item/42b571nn

المؤلفون: Feys, Simon, Vanmassenhove, Sam, Kraisin, Sirima, Yu, Karen, Jacobs, Cato, Boeckx, Bram, Cambier, Seppe, Cunha, Cristina, Debaveye, Yves, Gonçalves, Samuel M, Hermans, Greet, Humblet-Baron, Stephanie, Jansen, Sander, Lagrou, Katrien, Meersseman, Philippe, Neyts, Johan, Peetermans, Marijke, Rocha-Pereira, Joana, Schepers, Rogier, Spalart, Valérie, Starick, Marick R, Thevissen, Karin, Van Brussel, Thomas, Van Buyten, Tina, Van Mol, Pierre, Vandenbriele, Christophe, Vanderbeke, Lore, Wauters, Els, Wilmer, Alexander, Van Weyenbergh, Johan, Van De Veerdonk, Frank L, Carvalho, Agostinho, Proost, Paul, Martinod, Kimberly, Lambrechts, Diether, Wauters, Joost

المصدر: In The Lancet Microbe March 2024 5(3):e247-e260

المؤلفون: Van Slambrouck, Jan, Schoenaers, Charlotte, Laenen, Lies, Jin, Xin, Beuselinck, Kurt, Verdonck, Ann, Wauters, Joost, Molenberghs, Geert, Vanaudenaerde, Bart M., Vos, Robin, Mombaerts, Peter, Lagrou, Katrien, Ceulemans, Laurens J.

المصدر: In Journal of Virological Methods January 2024 323

المؤلفون: Yerbanga, Isidore W., Nakanabo Diallo, Seydou, Rouamba, Toussaint, Resendiz-Sharpe, Agustin, Lagrou, Katrien, Denis, Olivier, Rodriguez-Villalobos, Hector, Montesinos, Isabel, Bamba, Sanata

المصدر: In Journal of Medical Mycology November 2023 33(4)

المؤلفون: Arendrup, Maiken, Jorgensen, Karin, Guinea, Jesus, Lagrou, Katrien, Chryssanthou, Erja, Hayette, Marie-Pierre, Barchiesi, Francesco, Lass Flörl, Cornelia, Hamal, Petr, Dannaoui, Eric, Chowdhary, Anuradha, Meletiadis, Joseph

مصطلحات موضوعية: Eucast, suceptibility testing, EUCAST, Life sciences :: Microbiology, Sciences du vivant :: Microbiologie

جغرافية الموضوع: international

الوصف: Background: Terbinafine resistance is increasingly reported in Trichophyton rubrum and Trichophyton interdigitale rendering susceptibility testing important particularly in non-responding cases. We performed a multicentre evaluation of a recently proposed modified EUCAST method implementing medium supplemented with chloramphenicol and cycloheximide (CC) to avoid contamination.Materials/methods: A blinded panel of wild-type and squalene epoxidase (SQLE) target gene mutant T. rubrum and T. interdigitale strains were distributed to 10 European laboratories. Susceptibility to terbinafine, itraconazole, voriconazole and amorolfine) were performed according to the E.Def 9.3.1 method with and without addition of chloramphenicol and cycloheximide (final concentrations 50 mg/L and 300 mg/L, respectively). Plates were incubated at 25 °C (one laboratory used 30 °C) for 5-7 days until sufficient growth. MICs were determined visually (ignoring trailing growth for itraconazole) and spectrophotometrically with 90% and 50% endpoints yielding a total of 7,829 MICs. A. flavus ATCC 204304 and A. flavus CNM-CM1813 were included as controls.Results: 100%/96% (voriconazole) and 84%/84% (itraconazole) MIC determinations fell within the QC ranges for the two QC strains, respectively, and 96%/92% terbinafine MICs fell in a 0.25-1 mg/L 3 two-fold-dilution range suggesting a high interlaboratory reproducibility. Across the six methods, the number of terbinafine MEs varied from 2 (2.6%) to 5 (6.6%) for T. rubrum and between 0 and 2 (2.0%) for T. interdigitale (lowest for the CC-method (2.6%-4.4%/ 0-1% for T. rubrum/T. interdigitale). The difference between the modes for the wt and mutant population were ≥7 two-fold-dilutions in all cases (Table). If excluding a I121M/V237I T. rubrum mutant, and two mixed T. interdigitale strains, the number of VMEs were CC visual: T. rubrum: 1/77 (1.3%), CC spec-90%: 3/68 (4.4%) and CC spec-50%: 1/76 (1.3%), and none for T. interdigitale. The activity of voriconazole, itraconazole and amorolfine were quite uniform against T. rubrum and T. interdigitale, but unacceptably wide MIC ranges were found for the visual and spec-90% inhibition methods for itraconazole (data not shown).Conclusions: Although none of the laboratories perform dermatophyte testing at a regular basis an acceptable interlaboratory agreement and good separation between SQLE wt and mutants were found, suggesting a robust performance of the proposed method.

وصف الملف: Mini-oral ePoster session

Relation: 30th ECCMID, Paris, France (du 18 au 21 avril 2020)

URL الوصول: https://orbi.uliege.be/handle/2268/244128