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المؤلفون: Yehuda Shoenfeld, Martin Cornillet, Sivan Vainer, Gidi Karmon, Smadar Gertel, Ora Shovman, Howard Amital, Guy Serre
المساهمون: Chaim Sheba Medical Center, Sackler Faculty of Medicine, Tel Aviv University [Tel Aviv], Unité différenciation épidermique et auto-immunité rhumatoïde (UDEAR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Toulouse [Toulouse], Tel Aviv University (TAU), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), CARBILLET, Véronique
المصدر: Mediators of Inflammation, Vol 2017 (2017)
Mediators of Inflammation
Mediators of Inflammation, Hindawi Publishing Corporation, 2017, 2017, 9 p. ⟨10.1155/2017/3916519⟩
Mediators of Inflammation, 2017, 2017, 9 p. ⟨10.1155/2017/3916519⟩مصطلحات موضوعية: Male, 0301 basic medicine, MESH: Fibrinogen / metabolism, MESH: Collagen Type II / metabolism, MESH: Arthritis, Rheumatoid / drug therapy, Filaggrin Proteins, Autoantigens, Epitope, Arthritis, Rheumatoid, Epitopes, MESH: Autoantigens / therapeutic use, 0302 clinical medicine, MESH: Arthritis, Rheumatoid / immunology, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Synovial Membrane / drug effects, MESH: Aged, MESH: Middle Aged, biology, Chemistry, Synovial Membrane, FOXP3, Citrullination, Middle Aged, MESH: Autoantibodies / metabolism, 3. Good health, MESH: Young Adult, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Autoantigens / immunology, Female, Antibody, Research Article, Filaggrin, lcsh:RB1-214, Adult, MESH: Leukocytes, Mononuclear / metabolism, MESH: Epitopes, Article Subject, CD3, Immunology, MESH: Autoantibodies / immunology, MESH: Autoantigens / chemistry, Proinflammatory cytokine, Immunomodulation, Young Adult, 03 medical and health sciences, MESH: Immunomodulation / physiology, lcsh:Pathology, Humans, Vimentin, Collagen Type II, Aged, Autoantibodies, 030203 arthritis & rheumatology, MESH: Vimentin / metabolism, MESH: Humans, Autoantibody, Fibrinogen, MESH: Adult, MESH: Synovial Membrane / metabolism, Cell Biology, MESH: Male, MESH: Leukocytes, Mononuclear / drug effects, 030104 developmental biology, Leukocytes, Mononuclear, biology.protein, MESH: Citrullination, MESH: Female
الوصف: Citrullinated peptides are used for measuring anticitrullinated protein antibodies (ACPA) in rheumatoid arthritis (RA). Accumulation of citrullinated proteins in the inflamed synovium suggests that they may be good targets for inducing peripheral tolerance. In view of the multiplicity of citrullinated autoantigens described as ACPA targets, we generated a multiepitope citrullinated peptide (Cit-ME) from the sequences of major citrullinated autoantigens: filaggrin, β-fibrinogen, vimentin, and collagen type II. We assessed the ability of Cit-ME or the citrullinated β60-74 fibrinogen peptide (β60-74-Fib-Cit) which bears immunodominant citrullinated epitopes (i) to modify cytokine gene expression and (ii) to modulate Treg and Th17 subsets in PBMC derived from newly diagnosed untreated RA patients. RA patient’s PBMC incubated with Cit-ME or β60-74-Fib-Cit, showed upregulation of TGF-β expression (16% and 8%, resp.), and increased CD4+Foxp3+ Treg (22% and 19%, resp.). Both peptides were shown to downregulate the TNF-α and IL-1β expression; in addition, Cit-ME reduced CD3+IL17+ T cells. We showed that citrullinated peptides can modulate the expression of anti- and proinflammatory cytokines in PBMC from RA patients as well as the proportions of Treg and Th17 cells. These results indicate that citrullinated peptides could be active in vivo and therefore might be used as immunoregulatory agents in RA patients.
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