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1دورية أكاديمية
المؤلفون: Dinar Arifianto, Anita Esfandiari, I Wayan Teguh Wibawan, Amrozi Amrozi, Maharani Maharani, Darsono Darsono, Hirawan Setiadi, Agus Setiyono
المصدر: Jurnal Sain Veteriner, Vol 42, Iss 1, Pp 24-31 (2024)
مصطلحات موضوعية: antisera, besi, hematologi, hiperimun, kuda, Veterinary medicine, SF600-1100
الوصف: Kuda merupakan salah satu hewan yang penting dalam produksi plasma antisera sebagai bahan baku pembuatan serum. Akhir abad ke-19 kuda berperan krusial dalam pengembangan serum pertama terhadap penyakit difteri manusia. Hingga saat ini kuda masih digunakan untuk memproduksi berbagai serum anti-venom, anti-rabies, anti-tetanus, hingga anti-SARS CoV-2. Proses produksi plasma antisera diawali dengan injeksi imunogen pada kuda yang disebut dengan hiperimunisasi. Antibodi dalam jumlah besar yang terbentuk dikoleksi kemudian dipurifikasi untuk digunakan pada manusia. Hiperimunisasi menimbulkan berbagai efek samping, diantaranya adalah penimbunan fibril amiloid pada berbagai jaringan yang dapat mengganggu fisiologi dan fungsi organ tubuh. Sebanyak 12 ekor kuda digunakan dalam penelitian ini dan dibagi menjadi 4 kelompok. Kuda kontrol (n=3) terdiri atas kuda yang belum pernah mendapatkan perlakuan hiperimunisasi, sedangkan 9 kuda lainnya dikelompokkan berdasarkan lamanya waktu produksi plasma antisera dengan metode hiperimunisasi (masing-masing kelompok berjumlah 3 ekor). Kelompok I terdiri atas kuda dengan masa produksi 2-3 tahun, kelompok II terdiri atas kuda dengan masa produksi 4-5 tahun, dan kelompok III terdiri atas kuda dengan masa produksi 6-7 tahun. Sampel darah, serum, dan aspirasi sumsum tulang dianalisis utuk menghitung niali eritrosit, Hb, PCV, trombosit, profil besi (besi serum, UIBC, TIBC, saturasi transferin), rasio mieloid:eritroid (M:E) dan persentase retikulosit. Analisis statistik menunjukkan bahwa terdapat perbedaan yang signifikan pada parameter eritrosit dan rasio M:E (P0,05). Data tersebut menunjukkan bahwa perlakuan hiperimunisasi menyebabkan penurunan eritrosit dan peningkatan nilai rasio mieloid:eritroid (M:E) pada kuda penghasil plasma antisera.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Andhika Guna Dharma, Arief Wildan, Maharani Maharani, Riski Prihatningtias, Fifin Luthfia Rahmi, Trilaksana Nugroho, Arnila Novitasari Saubig, Zahira Rikiandraswida
المصدر: World Nutrition Journal, Vol 7, Iss i2, Pp 18-25 (2024)
مصطلحات موضوعية: folic acid, homocysteine, vascular density, non-perfusion area, diabetic retinopathy, Nutrition. Foods and food supply, TX341-641
الوصف: Background : Diabetic Retinopathy (DR) is the most common microvascular complication of Diabetes Mellitus (DM). Homocysteine has been studied as a biomarker in DR, while folic acid exhibits anti-proliferative effects in DR. Objective : To analyze the correlation between folic acid and homocysteine serum with vascular density and retinal non-perfusion area in healthy individuals and patients with diabetic retinopathy. Methods : This is an observational study with a cross-sectional design, conducted in Dr. Kariadi Hospital and GAKI laboratory in Semarang in January 2023. This study included 60 samples: 15 healthy individuals, 15 patients with DM but no DR, 15 patients with Non-Proliferative Diabetic Retinopathy (NPDR), and 15 patients with Proliferative Diabetic Retinopathy (PDR). Patients were examined for serum folic acid and homocysteine using blood laboratory tests, vessel density and retinal non-perfusion areas using optical coherence tomography angiography (OCTA). Results : There was a negative correlation with weak strength between folic acid levels and retinal non-perfusion area of the retina in all samples (Folic acid levels vs retinal non-perfusion area, p = 0.009, Spearman correlation = -0.335). There was a positive correlation with weak strength between folic acid levels and vascular density in all samples (Folic acid levels vs vascular density, p = 0.009, Spearman correlation = 0.337). There was a positive correlation with moderate strength between homocysteine levels and retinal non-perfusion area in all samples (Homocysteine levels vs non–perfusion area of the retina, p = 0.001, Spearman correlation = 0.426). There was a positive correlation with moderate strength between homocysteine levels and vascular density in all samples (Homocysteine levels vs vascular density, p = 0.001, Spearman correlation = -0.414). Conclusion : There was a correlation between folic acid and homocysteine serum with vascular density and retinal non-perfusion areas.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Siti Soleha, Delima Engga maretha, Andi Saputra, Siti Rachmi Indahsari, Binsar Butar Butar, Ahma Adi Suhendra, Maharani Maharani, Harlis Harlis, Hari Kapli
المصدر: Jurnal Biota, Vol 9, Iss 2, Pp 72-79 (2023)
مصطلحات موضوعية: enzymatic activity, averrhoa bilimbi l, eco-enzyme, ph., Biology (General), QH301-705.5
الوصف: Enzymes are biocatalysts that have many benefits in the industry and the environment. Eco-enzymes are reported to have amylase, lipase, and protease activities. Enzymatic activity is strongly influenced by several factors, one of which is pH. This study aims to determine the optimum enzymatic activity (amylase, lipase and protease) of Averrhoa bilimbi L. fruit eco-enzymes with various pH treatments (5, 6, 7, 8). Enzymatic activity was measured using a spectrophotometric method. Amylase activity assay using starch as a substrate. Casein and para nitrophenol palmitate were used as substrates. Casein was used as a substrate in the protease activity assays. In the lipase activity assays, para nitrophenol palmitate was used as substrate. Data analysis results are presented in graphical and descriptive. Averrhoa bilimbi L. fruit eco-enzyme has a protein concentration of 0.459 mg/ml. The optimum activity of amylase at pH 5 was 11,713.871 U/mg. Optimum activity and protease activity occurred at pH 8 and 6 of 3.667 U/mg and 13,400.77 U/mg, respectively.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Sutrisno Sutrisno, Maharani Maharani
المصدر: The Scientific World Journal, Vol 2024 (2024)
مصطلحات موضوعية: Technology, Medicine, Science
الوصف: Endometriosis (EM) is a gynecological disorder that causes morbidity in women and is characterized by endometrial tissue in the uterus cavity. This study investigated the mechanism of genistein in the VEGF-A and ER-α expression through in vivo and in silico approaches. An in vivo study was conducted by thirty-six mice that were divided into six groups including control, EM, and EM treatment with genistein with the doses of 1.3, 1.95, 2.6, and 3.25 mg/day for 14 days. Peritoneal tissues with lesions were collected and analyzed by immunohistochemistry to measure the VEGF-A and ER-α expression. The data were analyzed using a statistical approach using one-way ANOVA followed by Tukey HSD test with a significant value p
وصف الملف: electronic resource
Relation: https://doaj.org/toc/1537-744X
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5دورية أكاديمية
المؤلفون: Dewi Murni, Sidaria Sidaria, Annisa Annisa, Maharani Maharani
المصدر: NERS Jurnal Keperawatan, Vol 18, Iss 1, Pp 1-7 (2022)
الوصف: Job satisfaction is an individual’s general attitude towards the work he does. Nurses’ job satisfaction will reflect the level where a nurse has responsibility and likes work. Selecting the work given or assigned will positively impact both the nurse herself and the patient. Job satisfaction is (job satisfaction) an affective or emotional response to various aspects of one's work. The factors that influence the job satisfaction of nurses are motivation, environment, and the role of the manager. This type of research is quantitative with a cross-sectional model approach. The population of this study was all nurses who served in the inpatient room of RSI Siti Rahmah Padang, with a total sample of 50 nurses. The sampling technique used is proportional stratified random sampling, which meets the sample criteria. Data was taken using a questionnaire containing the item, job satisfaction of nurses. The motivation variable is the dominant factor that affects the job satisfaction of nurses, with a value of = 0.007. It is hoped that the hospital will review the motivations that can increase the job satisfaction of nurses and the policies taken and make decisions. Keywords: Nurse Job Satisfaction, Motivation, Environment, Manager's Role
وصف الملف: electronic resource
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6دورية أكاديمية
المصدر: JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan), Vol 7, Iss 1, Pp 32-36 (2022)
مصطلحات موضوعية: Public aspects of medicine, RA1-1270
الوصف: Stunting is the growth and development of a child's height that is not the same as his age caused by malnutrition and lack of parental knowledge. The purpose of the study was to determine the effectiveness of counseling on maternal knowledge and attitudes in preventing stunting at the Bawomataluo Public Health Center in South Nias in 2021. The research method is a quasi-experimental design with one group pretest-posttest and a control group. The number of samples was 30 pregnant women, 15 intervention groups and 15 control groups. Methods of collecting data using a questionnaire with the Wilcoxon test. The results of statistical tests obtained knowledge and attitudes p value 0.001 (p
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: Maharani Maharani, Lia Lajuna, Cut Yuniwati, Oktalia Sabrida, Sutrisno Sutrisno
المصدر: Journal of Ayurveda and Integrative Medicine, Vol 12, Iss 2, Pp 229-233 (2021)
مصطلحات موضوعية: Peritoneum, Endometriosis lesions, Cell death, Proliferation, Flavonoids, Miscellaneous systems and treatments, RZ409.7-999
الوصف: Background: Endometriois represents a gynecological disease that still becomes an issue in community. Phaleria macrocarpa is a plant native to Indonesia that contains an antioxidant substance, which may serve as apoptotic modulator and useful for angiogenesis. Objective: This study aims to evaluate the effects of flavonoid isolates from P. macrocarpa (PM) on the development of granulomas, apoptosis, proliferation, and angiogenesis of the disease. Material and methods: Total thirty mice (Mus musculus) were categorized into six groups, including the normal group (without any treatment), EMT (endometriosis) group, and EMT group treated with PM flavonoid isolates. Identification of the active compounds of P. macrocarpa was done using LC-HRMS. Measurement of granuloma scores and vascular density was done histologically. Apoptosis and proliferation analysis was performed by immunohistochemical techniques. Results: There was an increase in granulomas, proliferation, and apoptosis in the peritoneal tissues of the endometriosis model. This change can be normalized by extract of P. macrocarpa. Conclusion: We concluded that the flavonoid isolates from P. macrocarpa can suppress the growth of endometriosis lesions through normalization of proliferation and apoptosis. Thus, the P. macrocarpa flavonoid can be used as an alternative to inhibit the development of endometriosis.
وصف الملف: electronic resource
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8دورية أكاديمية
المصدر: Hayati Journal of Biosciences, Vol 29, Iss 3 (2022)
مصطلحات موضوعية: Biology (General), QH301-705.5
الوصف: This study aimed to establish an allometric model for estimation of aboveground biomass, and carbon sequestration in A. lanata mangrove forest growing in Muna Regency, Southeast Sulawesi. Research methods were done by transect and 5 quadrats with size of 100 m2 each. A total of thirteen individual trees with different sizes were harvested. While DBH and D30 were measured. The samples were separated into stems, branches, and leaves and then weighted. The sample from each fresh organs were taken and brought to the Laboratory and then oven dried at 80°C for 7 days. The allometric equations were established by using independent variables (DBH and D30), and dependent variables (Ws, Wb, Wl). The partial and overall aboveground biomasses were calculated from allometric model, while carbon stock and CO2 sequestration were estimated. The results showed that the independent variable of DBH was more applicable for estimation of Ws, Wb, Wl, and total biomasses (Mg ha-1) of A. lanata forest, which were estimated as 28.28±3.48, 6.40±0.79, 5.00±0.66, and 40.08±4.97 respectively. The carbon stock in stems (13.24±1.63 Mg ha-1) was higher than in branches (3.01±0.37 Mg C ha-1) as well as in leaves (2.35±0.31 Mg C ha-1). The total of carbon stock were estimated at about 18.83±2.33 Mg C ha-1. Meanwhile, the total of CO2 absorption by A. lanata mangrove was 43.95±5.45 Mg CO2 ha-1. Therefore a regenerated A. lanata mangrove in this in-active pond area had potentiality on carbon stock and sequestrations, although these vegetation condition was still in the growth stage.
وصف الملف: electronic resource
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9دورية أكاديمية
المؤلفون: Maharani Maharani, Sri Wahyuni, Diah Fitrianti
المصدر: AcTion: Aceh Nutrition Journal, Vol 4, Iss 2, Pp 81-88 (2019)
مصطلحات موضوعية: balita, makanan tambahan, pengetahuan dan sikap, status gizi, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456
الوصف: The double burden of nutrition is very worrying for children in Indonesia. Indonesia has a prevalence of malnutrition of 13,8% and 3,1% was overweight of children, while in Aceh that is 16,8% and 2,9% of overweight. One of the factors is the arrangement of additional food that is not patterned. The purpose of this study was to examine the relationship between mother's knowledge and attitudes about supplementary food and nutritional status in infants. The study was an analytic survey using a cross sectional approach. Samples were taken as many as 86 toddlers with a simple random technique. Data collection using questionnaires and anthropometric measurements. Knowledge and attitude data are processed according to percentage values, and nutritional status data are processed according index to WFA. The statistical test is chi-square at CI:95%. The results showed that mothers' knowledge and attitudes about supplementary food were still not good, and there were still many toddlers with the best nutritional status. Other results show the relationship between maternal knowledge (p=0,000) and maternal attitudes (p=0,019) regarding supplementary food with nutritional status in toddlers in Woyla Barat District. Conclusions, poor mother's knowledge and attitude about supplementary feeding is related to the high problem of malnutrition and over nutrition. Suggestions, counseling is needed related to providing good and nutritious supplementary food, as well as monitoring the nutritional status of children continuously both in the WFA, HFA and WFH indexs. Beban ganda masalah gizi sangat mengkhawatirkan anak-anak di Indonesia. Indonesia mempunyai prevalensi kekurangan gizi sebesar 13,8% dan 3,1% balita gizi lebih, sedangkan di Aceh yaitu sebesar 16,8% dan 2,9% anak gizi lebih. Salah satu faktor penyebab yaitu pengaturan makanan tambahan yang tidak terpola. Tujuan penelitian untuk mengukut hubungan pengetahuan dan sikap ibu tentang makanan tambahan dengan status gizi pada balita di. Penelitian bersifat survey analitik dengan menggunakan pendekatan cross sectional. Sampel diambil sebanyak 86 balita dengan teknik acak sederhana. Pengumpulan data menggunakan kuesioner dan pengukuran antropometri. Data pengetahuan dan sikap diolah menurut nilai persetase, dan data status gizi diolah menurut indeks BB/U. Uji statistik yaitu chi-square pada CI:95%. Hasil penelitian menunjukan pengetahuan dan sikap ibu tentang makanan tambahan masih kurang baik, serta masih banyak balita yang bestatus gizi kurang. Hasil lainnya menunjukkan hubungan antara pengetahuan ibu (p= 0,000) dan sikap ibu (p= 0,019) tentang makanan tambahan dengan status gizi pada balita di Kecamatan Woyla Barat Kabupaten. Kesimpulan, pengetahuan dan sikap ibu yang kurang baik tentang pemberian makannan tambahan berhubungan dengan tingginya masalah gizi kurang dan kelebihan gizi. Saran, perlu dilakukan penyuluhan terkait pemberian makanan tambahan yang baik dan bergizi, serta pemantauan status gizi anak secara terus menerus baik pada indeks BB/U, TB/U dan BB/TB.
وصف الملف: electronic resource
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10دورية أكاديمية
المؤلفون: Reni Apriani, Maharani Maharani
المصدر: Jurnal Studi Sosial dan Politik, Vol 3, Iss 1, Pp 17-27 (2019)
مصطلحات موضوعية: local election, candidate, strategy, campaign, Political science (General), JA1-92, Social Sciences
الوصف: Election of Regional Heads of South Sumatra Province has been held in 2018 with 4 pairs of candidates participated and won by the couple Herman Deru and Mawardi Yahya. The interesting thing from this research is that there has been intense competition between the four Paslon. Where each candidate, previously served as regional head in several districts in South Sumatra. The victory of this couple did not escape the winning strategy that they have implemented. The problem discussed in this study is how the process of the campaign implemented by Herman Deru and Mawardi Yahya in the South Sumatra Regional Election in 2018 and what the winning strategy used by the Paslon. The researcher uses the strategy theory from Kotten where in this theory it is stated that there are 4 types of winning strategies namely organizational strategy, program strategy, resource strategy, and institutional strategy. This type of research is a qualitative descriptive study. Methods of collecting data from this study through interviews, observation, and documentation. The results of this study indicate that of the 4 types of winning strategies as proposed by Kotten, the four strategies have been implemented by the couple Herman Deru and Mawardi Yahya. The strategy they use is different from the strategies used by other partners such as organizational strategy. Specifically in the program section and how to campaign through social media. And there are 4 stages of the campaign process set by the South Sumatra KPU in the South Sumatra Regional Election in the 2018-2023 period, namely public debates, distribution of campaign materials, installation of campaign props, and campaign advertisements in the media.
وصف الملف: electronic resource
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