المؤلفون: Testera AM, Alonso M, Rodríguez-Cabello JC, Barbosa JS, Costa RR, Mano JF

المصدر: Nanoscale Research Letters, Vol 4, Iss 10, Pp 1247-1253 (2009)

مصطلحات موضوعية: Elastin-like polymer, Biodegradable polymers, Biomimetic, Smart coatings, Multi-layers, Self-assembling nano-layers, Tissue engineering, Biomaterials, LbL, Materials of engineering and construction. Mechanics of materials, TA401-492

الوصف: Abstract Electrostatic self-assembly was used to fabricate new smart multi-layer coatings, using a recombinant elastin-like polymer (ELP) and chitosan as the counterion macromolecule. The ELP was bioproduced, purified and its purity and expected molecular weight were assessed. Aggregate size measurements, obtained by light scattering of dissolved ELP, were performed as a function of temperature and pH to assess the smart properties of the polymer. The build-up of multi-layered films containing ELP and chitosan, using a layer-by-layer methodology, was followed by quartz-crystal microbalance with dissipation monitoring. Atomic force microscopy analysis permitted to demonstrate that the topography of the multi-layered films could respond to temperature. This work opens new possibilities for the use of ELPs in the fabrication of biodegradable smart coatings and films, offering new platforms in biotechnology and in the biomedical area.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/1931-7573; https://doaj.org/toc/1556-276X

URL الوصول: https://doaj.org/article/fa0d406544af46c6ac733ad777775b18

المؤلفون: Agnello, Stefano, Gasperini, L, Reis, RL, Mano JF, PITARRESI, Giovanna, PALUMBO, Fabio Salvatore, GIAMMONA, Gaetano

المساهمون: Agnello, S, Gasperini, L, Reis, RL, Mano JF, Pitarresi, G, Palumbo FS, Giammona G

مصطلحات موضوعية: Hyaluronic acid, microfibers, microfluidic technique, drug delivery system

URL الوصول: https://explore.openaire.eu/search/publication?articleId=od______3658::a992b76dbdc6d67e98c5521c69e314d6
http://hdl.handle.net/10447/165810

المؤلفون: Bishi DK, Gasperini L, Kroehne V, Marques AP, Mano JF, Reis RL

المصدر: TERM STEM 2014 NanoTools for diagnosis and regeneration of tissues with PT | Korea symposium.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=erc_________::5fc57a2d8646c2decf6adf788477ae91

المؤلفون: Joana Moreira-Silva, Afonso, D., Silva, Th, Volpato, F., Motta, A., Marques, Ap, Mano, Jf, Reis, Rl, Migliaresi, C.

المساهمون: Universidade do Minho

المصدر: ResearcherID

مصطلحات موضوعية: hASC, Patterning, Science & Technology, Fish scales

الوصف: Marine organisms and materials exhibit interesting properties for their use in biomedical applications, following biomimetic approaches [1,2]. For instance, the white seabass (Lates Calcarifer) scales exhibit a very interesting pattern, which inspire their use as a guiding platform for cellular growth, in a tissue regeneration approach. Moreover, fish scales are composed of hydroxyapatite and type I collagen fibrils, equivalent to the materials that one can find in human bone, as thus their use in bone tissue engineering is envisaged. The main goal of the present work was the assessment of fish scales, combining intrinsic features such as patterns, hydroxyapatite and collagen in different means, as cell culture supports aiming at guiding cell growth and extracellular matrix deposition and mineralization. In this sense, human adipose derived stem cells (hASCs) were cultured onto seabass scales, under osteogenic and non-osteogenic conditions. Fish scales supported cell adhesion and cytoskeleton organization defined by the surface patterning. Moreover, hASCs were able to proliferate along the time of culture and to differentiate towards the osteogenic lineage depositing and mineralizing the characteristic extracellular matrix. This work constitutes the first step to demonstrate the value of the intrinsic properties of seabass scales for exploitation in the biomedical field and in particular for bone tissue engineering.

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::8fda8b1222df11af3144720afe4a4ad7
https://hdl.handle.net/1822/24070

المؤلفون: Duarte, Ar, Joana Moreira-Silva, Silva, Th, Osinga, R., Ilan, M., Marques, Ap, Mano, Jf, Reis, Rl

المساهمون: Universidade do Minho

المصدر: ResearcherID

مصطلحات موضوعية: Scaffolds, Marine sponges, Tissue engineering

الوصف: Marine sponges possess remarkable structures, with highly interconnected pores, making them excellent candidates as nature made scaffolds with potential use in tissue engineering strategies. In addition they also exhibit interesting high swelling degree and a skeleton composed mainly of collagen fibres, in some species with embedded siliceous or carbonate spicules. In the present study, several marine sponges (Dysidea avara, Chondrosia remiformis, Axinella damicornis, Petrosia ficiformis, Agelas oroides, Sarcotragus spinosulus, and Psammocinia sp.) were decellularized by supercritical fluids, for further use as natural scaffolds for osteoblasts. Supercritical fluid extraction was performed at 200 bar and 40 °C for 6 hours, in the presence of ethanol (40%), which was used as a co-solvent. The morphology of sponges, namely porosity interconnectivity and mean pore size, was analysed by SEM and micro-CT. To evaluate decellularization, sponges before and after treatment were analysed in sections stained with Hematoxylin- Eosin. Cellularization experiments were performed in S. spinosulus, using Saos-2 cell line. Cells were seeded on the sponge, with further evaluation of cell morphology by SEM, cell viability by Live/dead cell staining together with phalloidin.and cell proliferation by DNA quantification. The results of in vitro tests demonstrate the success of the proposed methodology for the production of natural scaffolds from marine sponges, namely for Sarcotragus spinosulus.

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::16221548277bdaba6cfd0e6f17f4e96d
https://hdl.handle.net/1822/23998

المؤلفون: ESPIRITO SANTO, V, Frias, A, Carida', M, Cancedda, Ranieri, Gomes, Me, Mano, Jf, Reis, Rl

URL الوصول: https://explore.openaire.eu/search/publication?articleId=od______3710::427c452cbcda56349c486f00e1d934a1
http://hdl.handle.net/11567/219370

المؤلفون: Mano, Jf, Oliveira, Jt, Alves, Cm, Malafaya, Pb, Reis, Rl

المساهمون: Universidade do Minho

المصدر: Authenticus
CIÊNCIAVITAE

مصطلحات موضوعية: Science & Technology

الوصف: [Excerpt] Tissue engineering (TE) has emerged in the last decade of the 20th century as an alternative approach to circumvent the existent limitations in the current therapies for organ failure or replacement. The EU HIPPOCRATES project joined academic and industrial partners to develop novel products and concepts that can be used for bone, cartilage or osteochondral TE strategies. Several issues were addressed including i) the choice and study of adequate human cell cultures, (ii) the development of culture technology with which human tissues can be grown ex vivo in three dimensional biodegradable matrices, (iii) the development of a material technology with which polymeric matrices can be produced, being suitable for cell culture (proliferation, differentiation), (iv) to assess the in-vivo functionality and clinical relevance of the tissue engineering strategies. [...]
info:eu-repo/semantics/publishedVersion

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::0c00159ff18459bab991f740a3ef8632
https://hdl.handle.net/1822/59198

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