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1دورية أكاديمية
المؤلفون: Melissa C. Piontek, Wouter H. Roos
المصدر: Journal of Extracellular Biology, Vol 1, Iss 12, Pp n/a-n/a (2022)
مصطلحات موضوعية: atomic force microscopy (AFM), chylomicrons (CM), high‐density lipoproteins (HDL), lipoproteins (LPs), low‐density lipoproteins (LDL), mechanical properties, Cytology, QH573-671
الوصف: Abstract Lipoproteins (LPs) are micelle‐like structures with a similar size to extracellular vesicles (EVs) and are therefore often co‐isolated, as intensively discussed within the EV community. LPs from human blood plasma are of particular interest as they are responsible for the deposition of cholesterol ester and other fats in the artery, causing lesions, and eventually atherosclerosis. Plasma lipoproteins can be divided according to their size, density and composition into chylomicrons (CM), very‐low‐density lipoproteins (VLDL), low‐density lipoproteins (LDL) and high‐density lipoproteins (HDL). Here, we use atomic force microscopy for mechanical characterization of LPs. We show that the nanoindentation approach used for EV analysis can also be used to characterize LPs, revealing specific differences between some of the particles. Comparing LPs with each other, LDL exhibit a higher bending modulus as compared to CM and VLDL, which is likely related to differences in cholesterol and apolipoproteins. Furthermore, CM typically collapse on the surface after indentation and HDL exhibit a very low height after surface adhesion both being indications for the presence of LPs in an EV sample. Our analysis provides new systematic insights into the mechanical characteristics of LPs.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2768-2811
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2دورية أكاديمية
المؤلفون: Daan Vorselen, Susan M. van Dommelen, Raya Sorkin, Melissa C. Piontek, Jürgen Schiller, Sander T. Döpp, Sander A. A. Kooijmans, Brigitte A. van Oirschot, Birgitta A. Versluijs, Marc B. Bierings, Richard van Wijk, Raymond M. Schiffelers, Gijs J. L. Wuite, Wouter H. Roos
المصدر: Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
مصطلحات موضوعية: Science
الوصف: Red blood cell disorders are often accompanied by increased release of extracellular vesicles (EVs), but their structural and mechanical properties are not fully understood. Here, the authors show that red blood cell EVs show liposome-like mechanical features and are softened in blood disorder patients.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2041-1723
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3دورية أكاديمية
المؤلفون: Daan Vorselen, Melissa C. Piontek, Wouter H. Roos, Gijs J. L. Wuite
المصدر: Frontiers in Molecular Biosciences, Vol 7 (2020)
مصطلحات موضوعية: (small) vesicles, liposomes, extracellular vesicles, atomic force microscopy (AFM), nanoindentation, bending modulus, Biology (General), QH301-705.5
الوصف: Both natural as well as artificial vesicles are of tremendous interest in biology and nanomedicine. Small vesicles (
وصف الملف: electronic resource
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المؤلفون: Raya Sorkin, Rashmi Voleti, Josep Rizo, Guy Brand, Emma Logtenberg, Melissa C. Piontek, Gijs J.L. Wuite, Wouter H. Roos, Margherita Marchetti, Alexander J. Groffen, Emma Kerklingh
المساهمون: Physics of Living Systems, LaserLaB - Molecular Biophysics, Functional Genomics, Molecular Biophysics, Human genetics, Amsterdam Neuroscience - Cellular & Molecular Mechanisms
المصدر: Sorkin, R, Marchetti, M, Logtenberg, E, Piontek, M C, Kerklingh, E, Brand, G, Voleti, R, Rizo, J, Roos, W H, Groffen, A J & Wuite, G J L 2020, ' Synaptotagmin-1 and Doc2b Exhibit Distinct Membrane-Remodeling Mechanisms ', Biophysical Journal, vol. 118, no. 3, pp. 643-656 . https://doi.org/10.1016/j.bpj.2019.12.021
Biophysical Journal, 118(3), 643-656. Biophysical Society
Biophysical Journal, 118(3), 643-656. CELL PRESS
Biophys Jمصطلحات موضوعية: Biophysics, chemistry.chemical_element, Nerve Tissue Proteins, Calcium, Neurotransmission, SYT1, Membrane Fusion, Synaptic Transmission, Synaptotagmin 1, 03 medical and health sciences, 0302 clinical medicine, Humans, SDG 7 - Affordable and Clean Energy, 030304 developmental biology, 0303 health sciences, Fusion, Chemistry, Tethering, Calcium-Binding Proteins, Articles, DOC2B, Membrane, Synaptotagmin I, SNARE Proteins, 030217 neurology & neurosurgery, Protein Binding
الوصف: Synaptotagmin-1 (Syt1) is a calcium sensor protein that is critical for neurotransmission and is therefore extensively studied. Here, we use pairs of optically trapped beads coated with SNARE-free synthetic membranes to investigate Syt1-induced membrane remodeling. This activity is compared with that of Doc2b, which contains a conserved C2AB domain and induces membrane tethering and hemifusion in this cell-free model. We find that the soluble C2AB domain of Syt1 strongly affects the probability and strength of membrane-membrane interactions in a strictly Ca2+- and protein-dependent manner. Single-membrane loading of Syt1 yielded the highest probability and force of membrane interactions, whereas in contrast, Doc2b was more effective after loading both membranes. A lipid-mixing assay with confocal imaging reveals that both Syt1 and Doc2b are able to induce hemifusion; however, significantly higher Syt1 concentrations are required. Consistently, both C2AB fragments cause a reduction in the membrane-bending modulus, as measured by a method based on atomic force microscopy. This lowering of the energy required for membrane deformation may contribute to Ca2+-induced fusion.
وصف الملف: application/pdf
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المؤلفون: Rafael B. Lira, Melissa C. Piontek, Wouter H. Roos
المساهمون: Molecular Biophysics
المصدر: Biochimica et Biophysica Acta-General Subjects, 1865(4):129486. ELSEVIER SCIENCE BV
مصطلحات موضوعية: 0301 basic medicine, electroporation, Membrane biology, Biophysics, Nanotechnology, Mechanical properties, 02 engineering and technology, budding, Microscopy, Atomic Force, Biochemistry, Exosome, Micropipette aspiration (MPA), 03 medical and health sciences, Mechanobiology, Biomimetic Materials, membrane biology, Animals, Humans, exosome, Optical tweezers (OT), controlled study, Vesicles, Molecular Biology, Liposome, atomic force microscopy, aspiration, nanotechnology, optical tweezers, Vesicle, Cell Membrane, Optical Imaging, article, Electrodeformation, 021001 nanoscience & nanotechnology, Characterization (materials science), Biomechanical Phenomena, 030104 developmental biology, Membrane, Targeted drug delivery, Atomic force microscopy (AFM), Liposomes, liposome, micropipette, 0210 nano-technology, mechanics
الوصف: Background The interest in mechanics of synthetic and biological vesicles has been continuously growing during the last decades. Liposomes serve as model systems for investigating fundamental membrane processes and properties. More recently, extracellular vesicles (EVs) have been investigated mechanically as well. EVs are widely studied in fundamental and applied sciences, but their material properties remained elusive until recently. Elucidating the mechanical properties of vesicles is essential to unveil the mechanisms behind a variety of biological processes, e.g. budding, vesiculation and cellular uptake mechanisms. Scope of review The importance of mechanobiology for studies of vesicles and membranes is discussed, as well as the different available techniques to probe their mechanical properties. In particular, the mechanics of vesicles and membranes as obtained by nanoindentation, micropipette aspiration, optical tweezers, electrodeformation and electroporation experiments is addressed. Major conclusions EVs and liposomes possess an astonishing rich, diverse behavior. To better understand their properties, and for optimization of their applications in nanotechnology, an improved understanding of their mechanical properties is needed. Depending on the size of the vesicles and the specific scientific question, different techniques can be chosen for their mechanical characterization. General significance Understanding the mechanical properties of vesicles is necessary to gain deeper insight in the fundamental biological mechanisms involved in vesicle generation and cellular uptake. This furthermore facilitates technological applications such as using vesicles as targeted drug delivery vehicles. Liposome studies provide insight into fundamental membrane processes and properties, whereas the role and functioning of EVs in biology and medicine are increasingly elucidated.
وصف الملف: application/pdf
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المؤلفون: Susan M. van Dommelen, Raya Sorkin, Richard van Wijk, Marc Bierings, Birgitta Versluijs, Jürgen Schiller, Raymond M. Schiffelers, Brigitte A. van Oirschot, Sander T. Döpp, Daan Vorselen, Gijs J.L. Wuite, Melissa C. Piontek, Wouter H. Roos, Sander A.A. Kooijmans
المساهمون: Molecular Biophysics, LaserLaB - Molecular Biophysics, Physics of Living Systems
المصدر: Nature Communications, 9(1):4960. Nature Publishing Group
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Nature Communications
Nature Communications, 9(1). Nature Publishing Group
Vorselen, D, van Dommelen, S M, Sorkin, R, Piontek, M C, Schiller, J, Döpp, S T, Kooijmans, S A A, van Oirschot, B A, Versluijs, B A, Bierings, M B, van Wijk, R, Schiffelers, R M, Wuite, G J L & Roos, W H 2018, ' The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis ', Nature Communications, vol. 9, 4960, pp. 1-9 . https://doi.org/10.1038/s41467-018-07445-x
Nature Communications, 9:4960, 1-9. Nature Publishing Groupمصطلحات موضوعية: 0301 basic medicine, Erythrocytes, Chemistry(all), Membrane Fluidity, Science, General Physics and Astronomy, Spherocytosis, Hereditary, Physics and Astronomy(all), Microscopy, Atomic Force, Biochemistry, Extracellular vesicles, Article, General Biochemistry, Genetics and Molecular Biology, Hereditary spherocytosis, Extracellular Vesicles, 03 medical and health sciences, SDG 3 - Good Health and Well-being, medicine, Humans, Ankyrin, lcsh:Science, chemistry.chemical_classification, Liposome, Multidisciplinary, Biochemistry, Genetics and Molecular Biology(all), Atomic force microscopy, Erythrocyte Membrane, Proteins, General Chemistry, medicine.disease, Red blood cell, 030104 developmental biology, medicine.anatomical_structure, Membrane, chemistry, Drug delivery, Biophysics, lcsh:Q, Genetics and Molecular Biology(all)
الوصف: Extracellular vesicles (EVs) are widely studied regarding their role in cell-to-cell communication and disease, as well as for applications as biomarkers or drug delivery vehicles. EVs contain membrane and intraluminal proteins, affecting their structure and thereby likely their functioning. Here, we use atomic force microscopy for mechanical characterization of erythrocyte, or red blood cell (RBC), EVs from healthy individuals and from patients with hereditary spherocytosis (HS) due to ankyrin deficiency. While these EVs are packed with proteins, their response to indentation resembles that of fluid liposomes lacking proteins. The bending modulus of RBC EVs of healthy donors is ~15 kbT, similar to the RBC membrane. Surprisingly, whereas RBCs become more rigid in HS, patient EVs have a significantly (~40%) lower bending modulus than donor EVs. These results shed light on the mechanism and effects of EV budding and might explain the reported increase in vesiculation of RBCs in HS patients.
Red blood cell disorders are often accompanied by increased release of extracellular vesicles (EVs), but their structural and mechanical properties are not fully understood. Here, the authors show that red blood cell EVs show liposome-like mechanical features and are softened in blood disorder patients.وصف الملف: application/pdf; image/pdf
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المؤلفون: Melissa C. Piontek, Daan Vorselen, Gijs J.L. Wuite, Wouter H. Roos
المساهمون: Physics of Living Systems, LaserLaB - Molecular Biophysics, Molecular Biophysics
المصدر: Frontiers in Molecular Biosciences, 7(July):139, 1-14. Frontiers Media S.A.
Frontiers in Molecular Biosciences, 7:139. Frontiers Media S.A.
Frontiers in Molecular Biosciences, Vol 7 (2020)
Frontiers in Molecular Biosciences
Vorselen, D, Piontek, M C, Roos, W H & Wuite, G J L 2020, ' Mechanical Characterization of Liposomes and Extracellular Vesicles, a Protocol ', Frontiers in Molecular Biosciences, vol. 7, no. July, 139, pp. 1-14 . https://doi.org/10.3389/fmolb.2020.00139مصطلحات موضوعية: liposomes, 0301 basic medicine, Materials science, nanoindentation, SUPPORTED LIPID-BILAYERS, Canham-Helfrich theory, mechanical properties, (small) vesicles, CURVATURE, Biochemistry, Genetics and Molecular Biology (miscellaneous), Biochemistry, ELASTIC PROPERTIES, 03 medical and health sciences, 0302 clinical medicine, SDG 3 - Good Health and Well-being, Molecular Biosciences, Technology and Code, atomic force microscopy (AFM), lcsh:QH301-705.5, Molecular Biology, ATOMIC-FORCE MICROSCOPY, EXOSOMES, Liposome, SPECTROSCOPY, bending modulus, Vesicle, Force spectroscopy, RIGIDITY, BENDING STIFFNESS, Nanoindentation, Microvesicles, Characterization (materials science), stomatognathic diseases, 030104 developmental biology, lcsh:Biology (General), MICA, 030220 oncology & carcinogenesis, Drug delivery, Biophysics, Nanomedicine, extracellular vesicles, MEMBRANE-VESICLES
الوصف: Both natural as well as artificial vesicles are of tremendous interest in biology and nanomedicine. Small vesicles (
وصف الملف: application/pdf
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::593dffd332f9c9f9dc8c430314db4863
https://doi.org/10.3389/fmolb.2020.00139 -
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المساهمون: Molecular Biophysics, Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
المصدر: Biophysical Journal, 118(3). CELL PRESS
مصطلحات موضوعية: Stromal cell, Chemistry, Biophysics, Adipose tissue, Extracellular vesicles, Cell biology
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المؤلفون: Margherita Marchetti, Emma Kerklingh, Wouter H. Roos, Raya Sorkin, Josep Rizo, Melissa C. Piontek, Emma Logtenberg, Gijs J.L. Wuite, Alexander J. Groffen, Rashmi Voleti, Guy Brand
مصطلحات موضوعية: 0303 health sciences, Chemistry, 010402 general chemistry, SYT1, 01 natural sciences, Synaptotagmin 1, 0104 chemical sciences, DOC2B, Coupling (electronics), Membrane bending, 03 medical and health sciences, Membrane, Cytoplasm, Biophysics, Secretion, 030304 developmental biology
الوصف: While the role of Synaptotagmin-1 in living cells has been described in detail, it remains a challenge to dissect the contribution of membrane remodelling by its two cytoplasmic C2 domains (C2AB) to the Ca2+-secretion coupling mechanism. Here, we study membrane remodeling using pairs of optically-trapped beads coated with SNARE-free synthetic membranes. We find that the soluble C2AB domain of Syt1 strongly affects the probability and strength of membrane-membrane interactions in a strictly Ca2+- and protein-dependent manner. A lipid mixing assay with confocal imaging reveals that at low Syt1 concentrations, no hemifusion is observed. Notably, for similar low concentrations of Doc2b hemifusion does occur. Consistently, both C2AB fragments cause a reduction in the membrane bending modulus, as measured by an AFM-based method. This lowering of the energy required for membrane deformation likely contributes to the overall Ca2+-secretion triggering mechanism by calcium sensor proteins. When comparing symmetrical (both sides) and asymmetrical (one side) presence of protein on the membranes, Syt1 favors an asymmetrical but Doc2b a symmetrical configuration, as inferred from higher tether probabilities and break forces. This provides support for the direct bridging hypothesis for Syt-1, while hinting to possible preference for protein-protein (and not protein-membrane) interactions for Doc2b. Overall, our study sheds new light on the mechanism of Ca2+ induced fusion triggering, which is essential for fundamental understanding of secretion of neurotransmitters and endocrine substances.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abd24d18c75c780c62b1e4b9db485073
https://doi.org/10.1101/538405 -
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المؤلفون: Melissa C, Piontek, Wouter H, Roos
المصدر: Methods in molecular biology (Clifton, N.J.). 1665
مصطلحات موضوعية: Nanotechnology, Proteins, Microscopy, Atomic Force
الوصف: Imaging of nano-sized particles and sample features is crucial in a variety of research fields. For instance in biological sciences, where it is paramount to investigate structures at the single particle level. Often two-dimensional images are not sufficient and further information such as topography and mechanical properties are required. Furthermore, to increase the biological relevance, it is desired to perform the imaging in close to physiological environments. Atomic force microscopy (AFM) meets these demands in an all-in-one instrument. It provides high-resolution images including surface height information leading to three-dimensional information on sample morphology. AFM can be operated both in air and in buffer solutions. Moreover, it has the capacity to determine protein and membrane material properties via the force spectroscopy mode. Here we discuss the principles of AFM operation and provide examples of how biomolecules can be studied. By including new approaches such as high-speed AFM (HS-AFM) we show how AFM can be used to study a variety of static and dynamic single biomolecules and biomolecular assemblies.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=pmid________::470a61799fbec311460d628c23a15d67
https://pubmed.ncbi.nlm.nih.gov/28940073
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